Introduction:Transplantation of mesenchymal stromal cells(MSCs)is a promising therapy for type 1 diabetes(T1D).However,whether the infused MSCs affect the endoplasmic reticulum stress or subsequent unfolded protein re...Introduction:Transplantation of mesenchymal stromal cells(MSCs)is a promising therapy for type 1 diabetes(T1D).However,whether the infused MSCs affect the endoplasmic reticulum stress or subsequent unfolded protein response inβcells remains unclear.Methods:To investigate this,we induced early-onset T1D in non-obese diabetic mice using streptozotocin.Subsequently,T1D mice were randomly assigned to receive either MSCs or phosphate-buffered saline.We observed the in vivo homing of MSCs and assessed their effectiveness by analyzing blood glucose levels,body weight,histopathology,pancreatic protein expression,and serum levels of cytokines,proinsulin,and C-peptide.Results:Infused MSCs were found in the lungs,liver,spleen,and pancreas of T1D mice.They exhibited various effects,including reducing blood glucose levels,regulating immunity,inhibiting inflammation,increasingβ-cell areas,and reducing the expression of key proteins in the unfolded protein response pathway.Fasting serum proinsulin and C-peptide levels were significantly higher in the MSCs treatment group than in the T1D model group.However,there was no significant difference in the biomarker ofβ-cell endoplasmic reticulum stress,the ratio of fasting serum proinsulin to C-peptide,between the two groups.Conclusion:Ourfindings reveal that MSCs infusion does not alleviate endoplasmic reticulum stress inβcells directly but modulates the unfolded protein response pathway to preserveβ-cell mass and function in T1D mice.展开更多
The authors regret that the last name and first name of all authors are reversed in the original article.The correct writing is as follows:Le Jiang,a,b#;Zhongqun Liu,a,b#;Zhaoyan Wang,d,e,f;Yijun Su,a,b;Yingjin Wang,a...The authors regret that the last name and first name of all authors are reversed in the original article.The correct writing is as follows:Le Jiang,a,b#;Zhongqun Liu,a,b#;Zhaoyan Wang,d,e,f;Yijun Su,a,b;Yingjin Wang,a,b;Yaojie Wei,a,b;Yanan Jiang,c;Zhanrong Jia,c;Chunyang Ma,a,b;Fangli Gang,a,b;Nan Xu,a,b;Lingyun Zhao,a,b;Xiumei Wang,a,b;Qiong Wu,d,e,f;Xiong Lu,c,*and Xiaodan Sun,a,b,*a.State Key Laboratory of New Ceramics and Fine Processing,School of Materials Science and Engineering,Tsinghua University,Beijing 100084,People’s Republic of China;b.Key Laboratory of Advanced Materials of Ministry of Education of China,School of Materials Science and Engineering,Tsinghua University,Beijing 100084,People’s Republic of China;c.Key Lab of Advanced Technologies of Materials of Ministry of Education,School of Materials Science and Engineering,Southwest Jiaotong University,Chengdu 610031,People’s Republic of China d.MOE Key Laboratory of Bioinformatics,Tsinghua University,Beijing,100084,People’s Republic of China e.Center for Synthetic and Systems Biology,Tsinghua University,Beijing,100084,People’s Republic of China f.School of Life Sciences,Tsinghua University,Beijing,100084,People’s Republic of China.展开更多
Soil loss, both from surface soil loss and subsurface soil leakage, in the karst regions of southwestern China is a serious environmental problem that threatens sustainability in that region. The surface soil loss has...Soil loss, both from surface soil loss and subsurface soil leakage, in the karst regions of southwestern China is a serious environmental problem that threatens sustainability in that region. The surface soil loss has been extensively studied, and many studies have been conducted to investigate the causes, impacts and mechanisms involved, but the study of subsurface soil leakage has received little attention due to the difficulties in studying the natural conditions. There is no consensus on the overall proportions between surface soil loss and subsurface soil leakage. To control soil loss, improve ecological restoration, and help locals out of poverty, the Chinese government carried out a series of ecological restoration projects in the karst regions of southwestern China starting in the 1980s. As a result, the intensity and areal extent of soil loss continues to decrease and the ecological situation is steadily improving. However, because of the fragile ecosystem in the karst regions, the soil loss control is a long-term task, and the soil loss in some karst regions continues to be a problem. Subsequently, we put forward some suggestions for the policy makers relative to conservation of soil loss and vegetation restoration. These suggestions include:(1) government, private organizations and individuals are encouraged to raise funds for soil loss control and vegetation restoration;(2) nature reserves should be established to increase biodiversity;(3) engineering projects such as small reservoirs, ponds, and flow diversion channels should be constructed in marginal karst regions.展开更多
An anomalous enhancement of fluorescence of carbon dots （CDs） was observed via lanthanum （La） doping. La-doped CDs （La-CDs） were prepared through microwave pyrolysis within 4 min. With La^3＋ doping, the emissio...An anomalous enhancement of fluorescence of carbon dots （CDs） was observed via lanthanum （La） doping. La-doped CDs （La-CDs） were prepared through microwave pyrolysis within 4 min. With La^3＋ doping, the emission band shifted from blue to green although LaB＋ is non-fluorescent. The quantum yield and fluorescence lifetime improved by about 20% and 35%, respectively. All experiment results indicate that La^3＋ doping is an effective way to tune fluorescence and improve the performance of CDs. Another unique attribute of La-CDs is high sensitivity to Fe^3＋. The La-CD-based fluorescence probe was established and used for sensitive and selective detection of Fe^3＋ with a limit of detection of 91 nmol/L. The proposed fluorescence probe also was successfully employed to visualize intracellular Fe^3＋ in live HeLa cells through cell imaging. It was also shown that yttrium exhibited the same fluorescence enhancement effect as La. The results may provide a new route for preparing CDs with special properties.展开更多
Sporadic vestibular schwannoma (acoustic neuroma) is a benign tumor arising from cochleovestibular nerve. Nowadays, various specialties and medical centers are treating this dis-ease, and the multidisciplinary collabo...Sporadic vestibular schwannoma (acoustic neuroma) is a benign tumor arising from cochleovestibular nerve. Nowadays, various specialties and medical centers are treating this dis-ease, and the multidisciplinary collaboration is the trend. In an effort to promote a uniform stan-dard for reporting clinical results, even for treatment indications, the mainly controversies were posed and discussed during the 7th International Conference on acoustic neuroma, and the agreement was summarized by the Committee of this conference. The main symptoms grading and tumor stage should note its name of classification for making them comparable. The goal of the modern managements for vestibular schwannoma is to improve the quality of life with lower mortality, lower morbidity and better neurological function preservation. The experience of surgical team and their preference might be a major factor for the outcome. Because of lack-ing of long-term follow-up large data after radiotherapy, and with the development of microsur-gery, radiotherapy is now less recommended except for recurrent cases or elderly patients.展开更多
文摘Introduction:Transplantation of mesenchymal stromal cells(MSCs)is a promising therapy for type 1 diabetes(T1D).However,whether the infused MSCs affect the endoplasmic reticulum stress or subsequent unfolded protein response inβcells remains unclear.Methods:To investigate this,we induced early-onset T1D in non-obese diabetic mice using streptozotocin.Subsequently,T1D mice were randomly assigned to receive either MSCs or phosphate-buffered saline.We observed the in vivo homing of MSCs and assessed their effectiveness by analyzing blood glucose levels,body weight,histopathology,pancreatic protein expression,and serum levels of cytokines,proinsulin,and C-peptide.Results:Infused MSCs were found in the lungs,liver,spleen,and pancreas of T1D mice.They exhibited various effects,including reducing blood glucose levels,regulating immunity,inhibiting inflammation,increasingβ-cell areas,and reducing the expression of key proteins in the unfolded protein response pathway.Fasting serum proinsulin and C-peptide levels were significantly higher in the MSCs treatment group than in the T1D model group.However,there was no significant difference in the biomarker ofβ-cell endoplasmic reticulum stress,the ratio of fasting serum proinsulin to C-peptide,between the two groups.Conclusion:Ourfindings reveal that MSCs infusion does not alleviate endoplasmic reticulum stress inβcells directly but modulates the unfolded protein response pathway to preserveβ-cell mass and function in T1D mice.
文摘The authors regret that the last name and first name of all authors are reversed in the original article.The correct writing is as follows:Le Jiang,a,b#;Zhongqun Liu,a,b#;Zhaoyan Wang,d,e,f;Yijun Su,a,b;Yingjin Wang,a,b;Yaojie Wei,a,b;Yanan Jiang,c;Zhanrong Jia,c;Chunyang Ma,a,b;Fangli Gang,a,b;Nan Xu,a,b;Lingyun Zhao,a,b;Xiumei Wang,a,b;Qiong Wu,d,e,f;Xiong Lu,c,*and Xiaodan Sun,a,b,*a.State Key Laboratory of New Ceramics and Fine Processing,School of Materials Science and Engineering,Tsinghua University,Beijing 100084,People’s Republic of China;b.Key Laboratory of Advanced Materials of Ministry of Education of China,School of Materials Science and Engineering,Tsinghua University,Beijing 100084,People’s Republic of China;c.Key Lab of Advanced Technologies of Materials of Ministry of Education,School of Materials Science and Engineering,Southwest Jiaotong University,Chengdu 610031,People’s Republic of China d.MOE Key Laboratory of Bioinformatics,Tsinghua University,Beijing,100084,People’s Republic of China e.Center for Synthetic and Systems Biology,Tsinghua University,Beijing,100084,People’s Republic of China f.School of Life Sciences,Tsinghua University,Beijing,100084,People’s Republic of China.
基金This research was funded by National key research and devel-opment plan project(2016YFC0502403)the Open Research Funds of State Key Laboratoryof Simulation and Regulation of Water Cycle in River Basin, China Institute of Water Resources and Hydro-powerResearch(SKL2018CG04)the National Natural Sci-ence Foundation of China(51879281)
文摘Soil loss, both from surface soil loss and subsurface soil leakage, in the karst regions of southwestern China is a serious environmental problem that threatens sustainability in that region. The surface soil loss has been extensively studied, and many studies have been conducted to investigate the causes, impacts and mechanisms involved, but the study of subsurface soil leakage has received little attention due to the difficulties in studying the natural conditions. There is no consensus on the overall proportions between surface soil loss and subsurface soil leakage. To control soil loss, improve ecological restoration, and help locals out of poverty, the Chinese government carried out a series of ecological restoration projects in the karst regions of southwestern China starting in the 1980s. As a result, the intensity and areal extent of soil loss continues to decrease and the ecological situation is steadily improving. However, because of the fragile ecosystem in the karst regions, the soil loss control is a long-term task, and the soil loss in some karst regions continues to be a problem. Subsequently, we put forward some suggestions for the policy makers relative to conservation of soil loss and vegetation restoration. These suggestions include:(1) government, private organizations and individuals are encouraged to raise funds for soil loss control and vegetation restoration;(2) nature reserves should be established to increase biodiversity;(3) engineering projects such as small reservoirs, ponds, and flow diversion channels should be constructed in marginal karst regions.
基金The authors gratefully acknowledge the financial support from the National Natural Science Foundation of China （Nos. 21375005, 21575054 and 21527808）.
文摘An anomalous enhancement of fluorescence of carbon dots （CDs） was observed via lanthanum （La） doping. La-doped CDs （La-CDs） were prepared through microwave pyrolysis within 4 min. With La^3＋ doping, the emission band shifted from blue to green although LaB＋ is non-fluorescent. The quantum yield and fluorescence lifetime improved by about 20% and 35%, respectively. All experiment results indicate that La^3＋ doping is an effective way to tune fluorescence and improve the performance of CDs. Another unique attribute of La-CDs is high sensitivity to Fe^3＋. The La-CD-based fluorescence probe was established and used for sensitive and selective detection of Fe^3＋ with a limit of detection of 91 nmol/L. The proposed fluorescence probe also was successfully employed to visualize intracellular Fe^3＋ in live HeLa cells through cell imaging. It was also shown that yttrium exhibited the same fluorescence enhancement effect as La. The results may provide a new route for preparing CDs with special properties.
文摘Sporadic vestibular schwannoma (acoustic neuroma) is a benign tumor arising from cochleovestibular nerve. Nowadays, various specialties and medical centers are treating this dis-ease, and the multidisciplinary collaboration is the trend. In an effort to promote a uniform stan-dard for reporting clinical results, even for treatment indications, the mainly controversies were posed and discussed during the 7th International Conference on acoustic neuroma, and the agreement was summarized by the Committee of this conference. The main symptoms grading and tumor stage should note its name of classification for making them comparable. The goal of the modern managements for vestibular schwannoma is to improve the quality of life with lower mortality, lower morbidity and better neurological function preservation. The experience of surgical team and their preference might be a major factor for the outcome. Because of lack-ing of long-term follow-up large data after radiotherapy, and with the development of microsur-gery, radiotherapy is now less recommended except for recurrent cases or elderly patients.
