The direct and sensitized photodegradations of imidacloprid, 1-(6-chloro-3-pyridinylmethyl)-N-nitro-2-imidazolidinimine, were investigated in aqueous solution and with and without various photo-sensitizers. Results of...The direct and sensitized photodegradations of imidacloprid, 1-(6-chloro-3-pyridinylmethyl)-N-nitro-2-imidazolidinimine, were investigated in aqueous solution and with and without various photo-sensitizers. Results of the study revealed that the intensity of lamp-house and irradiation wavelength had significant effects on the photolysis of imidacloprid. Complete degradation of 20 mg/L imidacloprid in aqueous phase was observed in 40 min under ultraviolet(UV) irradiation system, suggesting the ultraviolet ray played significant role in direct photolysis of imidacloprid. The additions of various photo-sensitizers lead to improve the degradation efficiency of imidacloprid under the irradiation of black light fluorescent lamp. TiO 2 was the most efficient in the photo-catalytic degradation of imidacloprid among other photo-sensitizers in used this study. However, addition of acetone inhibited the photolysis of imidacloprid under the irradiation of UV, indicating the occurrence of competition between acetone and imidacloprid for photos. Mineralization of the imidacloprid was examined to clarify the final photochemical degradation products of the insecticide which were CO 2, Cl- and NO- 3. Complete photo-oxidation of nitrogen to NO- 3 occurred very slowly via the intermediate formation of NH+ 4 and NO- 2.展开更多
This study aims to reveal the role of metal ion transporter DMT1 in neurotoxicity induced by Aβ142 oligomers.We exposed SH-SY5Y cells(S-DMT1)stably overexpressing the DMT1 gene and SH-SY5Y cells(SGFP)overexpressing e...This study aims to reveal the role of metal ion transporter DMT1 in neurotoxicity induced by Aβ142 oligomers.We exposed SH-SY5Y cells(S-DMT1)stably overexpressing the DMT1 gene and SH-SY5Y cells(SGFP)overexpressing empty green fluorescent protein to AB.42 oligomers.CCK8 and Hoechst 33258 were used to detect the cell death rate and apoptotic rate.W estern blotting was used to detect the levels of apoptotic proteins caspase-3,Bax and Bcl-2.The results of CCK8 experiments showed that compared with SGFP cells,the number of viable cells in S-DMT1 cells after Aβ42 oligomer treatment was significantly reduced.Heochst33258 staining results showed that the rate of nuclear shrinkage in S-DMT1 cells treated with Aβ142 oligomer was significantly higher than that in SGFP group.Moreover,the levels of caspase-3 and Bax proteins in S-DMT1 cells were higher than those in SGFP group,while BcI-2 decreased after Aβ42 oligomers.DMT1 overexpression increases the toxic effects of Aβ42 oligomers by promoting apoptosis.4.Regulation of"P35/P25-CDK5-Tau"signaling pathway in hippocampi and prefrontal cortex of AD rats by electroacupuncture.展开更多
本研究应用聚合酶链式反应(PCR)技术对龙眼黄烷酮-3-羟化酶基因(Dlf3h)进行克隆;运用实时荧光定量PCR (Quantitative real time PCR)技术对Dlf3h基因在龙眼根、茎、叶组织中的表达进行分析;采用生物信息学方法对Dlf3h蛋白理化性质、信...本研究应用聚合酶链式反应(PCR)技术对龙眼黄烷酮-3-羟化酶基因(Dlf3h)进行克隆;运用实时荧光定量PCR (Quantitative real time PCR)技术对Dlf3h基因在龙眼根、茎、叶组织中的表达进行分析;采用生物信息学方法对Dlf3h蛋白理化性质、信号肽、亚细胞定位、亲疏水性、二级结构、三级结构等进行预测。结果表明:成功克隆获得一条长度为1 098 bp的Dlf3h基因,由366个氨基酸组成;该基因在根中表达量最高,其次为叶,在茎中表达量最低;生物信息学分析表明Dlf3h没有信号肽和跨膜结构,是一种可溶性亲水蛋白,定位于细胞核内;二级结构元件主要由α-螺旋和无规则卷曲组成,具有卷曲螺旋;Ramachandran评估表明应用SWISS-MODEL构建的三级结构模型可靠,其配体结合位点为217 His和275 His。本研究结果为进一步研究龙眼类黄酮合成的分子调控机制提供依据。展开更多
文摘The direct and sensitized photodegradations of imidacloprid, 1-(6-chloro-3-pyridinylmethyl)-N-nitro-2-imidazolidinimine, were investigated in aqueous solution and with and without various photo-sensitizers. Results of the study revealed that the intensity of lamp-house and irradiation wavelength had significant effects on the photolysis of imidacloprid. Complete degradation of 20 mg/L imidacloprid in aqueous phase was observed in 40 min under ultraviolet(UV) irradiation system, suggesting the ultraviolet ray played significant role in direct photolysis of imidacloprid. The additions of various photo-sensitizers lead to improve the degradation efficiency of imidacloprid under the irradiation of black light fluorescent lamp. TiO 2 was the most efficient in the photo-catalytic degradation of imidacloprid among other photo-sensitizers in used this study. However, addition of acetone inhibited the photolysis of imidacloprid under the irradiation of UV, indicating the occurrence of competition between acetone and imidacloprid for photos. Mineralization of the imidacloprid was examined to clarify the final photochemical degradation products of the insecticide which were CO 2, Cl- and NO- 3. Complete photo-oxidation of nitrogen to NO- 3 occurred very slowly via the intermediate formation of NH+ 4 and NO- 2.
文摘This study aims to reveal the role of metal ion transporter DMT1 in neurotoxicity induced by Aβ142 oligomers.We exposed SH-SY5Y cells(S-DMT1)stably overexpressing the DMT1 gene and SH-SY5Y cells(SGFP)overexpressing empty green fluorescent protein to AB.42 oligomers.CCK8 and Hoechst 33258 were used to detect the cell death rate and apoptotic rate.W estern blotting was used to detect the levels of apoptotic proteins caspase-3,Bax and Bcl-2.The results of CCK8 experiments showed that compared with SGFP cells,the number of viable cells in S-DMT1 cells after Aβ42 oligomer treatment was significantly reduced.Heochst33258 staining results showed that the rate of nuclear shrinkage in S-DMT1 cells treated with Aβ142 oligomer was significantly higher than that in SGFP group.Moreover,the levels of caspase-3 and Bax proteins in S-DMT1 cells were higher than those in SGFP group,while BcI-2 decreased after Aβ42 oligomers.DMT1 overexpression increases the toxic effects of Aβ42 oligomers by promoting apoptosis.4.Regulation of"P35/P25-CDK5-Tau"signaling pathway in hippocampi and prefrontal cortex of AD rats by electroacupuncture.
文摘本研究应用聚合酶链式反应(PCR)技术对龙眼黄烷酮-3-羟化酶基因(Dlf3h)进行克隆;运用实时荧光定量PCR (Quantitative real time PCR)技术对Dlf3h基因在龙眼根、茎、叶组织中的表达进行分析;采用生物信息学方法对Dlf3h蛋白理化性质、信号肽、亚细胞定位、亲疏水性、二级结构、三级结构等进行预测。结果表明:成功克隆获得一条长度为1 098 bp的Dlf3h基因,由366个氨基酸组成;该基因在根中表达量最高,其次为叶,在茎中表达量最低;生物信息学分析表明Dlf3h没有信号肽和跨膜结构,是一种可溶性亲水蛋白,定位于细胞核内;二级结构元件主要由α-螺旋和无规则卷曲组成,具有卷曲螺旋;Ramachandran评估表明应用SWISS-MODEL构建的三级结构模型可靠,其配体结合位点为217 His和275 His。本研究结果为进一步研究龙眼类黄酮合成的分子调控机制提供依据。
