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槲皮素拮抗雷公藤甲素诱导的小鼠肝损伤的机制研究 被引量:10
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作者 魏彩冰 周莲娣 +2 位作者 张家维 张起辉 陶崑 《四川大学学报(医学版)》 CAS CSCD 北大核心 2019年第5期684-688,共5页
目的探究槲皮素(quercetin,QE)对雷公藤甲素(triptolide,TP)诱发肝损伤的拮抗机制。方法取40只C57BL/6小鼠,随机平均分为正常对照组、TP模型组、低剂量QE(20 mg/kg)组和高剂量QE(80 mg/kg)组。低剂量QE组和高剂量QE组进行预处理给药,按0... 目的探究槲皮素(quercetin,QE)对雷公藤甲素(triptolide,TP)诱发肝损伤的拮抗机制。方法取40只C57BL/6小鼠,随机平均分为正常对照组、TP模型组、低剂量QE(20 mg/kg)组和高剂量QE(80 mg/kg)组。低剂量QE组和高剂量QE组进行预处理给药,按0.2 mL/10 g量连续灌胃10 d,2次/d,其余各组灌胃等量生理盐水。末次给药4 h后,除正常对照组外,各组单次灌胃500μg/kg TP进行造模。造模22 h后取血,处死小鼠,肝切片HE染色观察肝组织病理学改变;检测小鼠血清中谷草转氨酶(AST)和谷丙转氨酶(ALT)活性;测定肝匀浆液中还原型谷胱甘肽(GSH)、超氧化物歧化酶(SOD)和丙二醛(MDA)水平;采用ELISA方法检测肝匀浆液中白细胞介素(IL)-17、IL-10和IL-6分泌情况;Western blot测定Toll样受体4(TLR4)蛋白表达水平。结果与正常对照组比较, TP模型组肝小叶结构萎缩甚至消失,肝细胞坏死明显,炎性细胞浸润;小鼠血清中ALT、AST和MDA水平异常升高,SOD和GSH水平降低,IL-6和IL-17分泌水平升高,IL-10分泌水平下降,TLR4蛋白水平升高(P<0.05)。与TP模型组相比, QE组肝组织损伤和炎症细胞浸润减轻,血清中ALT、AST、MDA、IL-6和IL-17水平均下降,TLR4表达水平下调(P<0.05),且高剂量QE组改变更为明显(P<0.05,与低剂量QE组比较)。结论槲皮素可通过减轻氧化损伤促进抗氧化,调控细胞因子的分泌,减轻TP诱导的肝损伤。 展开更多
关键词 雷公藤甲素 槲皮素 肝损伤 细胞因子 氧化应激
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DESIGN OF IMPLANTABLE AXIAL-FLOW BLOOD PUMP AND NUMERICAL STUDIES ON ITS PERFORMANCE 被引量:16
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作者 FAN Hui-min HONG Fang-wen +3 位作者 zhou lian-di CHEN Yin-sheng YE Liang LIU Zhong-min 《Journal of Hydrodynamics》 SCIE EI CSCD 2009年第4期445-452,共8页
This article presents the design of a new implantable axial-flow blood pump. The special feature of the flow channel inside the blood pump is that the blood is driven by a big-small tandem impeller installed in the in... This article presents the design of a new implantable axial-flow blood pump. The special feature of the flow channel inside the blood pump is that the blood is driven by a big-small tandem impeller installed in the inner hole of the cylinder magnet of a brushless direct current motor. The inner hole makes the main flow channel possible, while the gap between the inner end of the stator and the outer end of the cylinder magnet gives the shape of the tributary flow channel. There is no motor magnet inside the main flow channel, therefore, more blood can pass through it. The gap of the tributary flow channel is very small, but the blood flow in it is not blocked. Thus, the efficiency is increased and the volume and weight of blood pump can be reduced greatly. The outer diameter, length and weight of the manufactured implantable axial-flow blood pump are 29.6 mm, 76 mm and 158 g, respectively. The impeller spins at the speed of 9000 rpm and can generate a pressure head of 100 mmHg and a flow rate of 8 L/rain. In an animal experiment, the blood pump has been successfully applied as a Ventricular Assist Device (VAD) in the chest of a small cow. Besides a mathematical model is established to simulate the flow inside an axial-flow blood pump of implantable VAD. The numerical studies on the performance of the implantable axial-flow blood pump are carried out by combining this mathematical model and the Fluent software. The numerical results agree well with those of experiments, with the maximum error less than 10%. 展开更多
关键词 implantable axial-flow blood pump CFD numerical prediction PERFORMANCE
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EXPERIMENTAL STUDY OF UNSTEADY CHARACTERISTICS OF TURBULENT MIXING LAYER FLOW OVER AN OPEN STEP AND A SQUARE-EDGED RIB
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作者 KE Feng Chen Han-ping +5 位作者 Liu Ying-zheng He You-sheng Wu Ming-lan zhou lian-di Tao Ming-de Liu Yu-lu 《Journal of Hydrodynamics》 SCIE EI CSCD 2007年第6期786-786,共1页
Spatio-temporal characteristics of the wall-bounded turbulent mixing layer flow over an open step and a square-edged rib were extensively studied in a low-speed wind tunnel. Simultaneous measurements of wall-pressure ... Spatio-temporal characteristics of the wall-bounded turbulent mixing layer flow over an open step and a square-edged rib were extensively studied in a low-speed wind tunnel. Simultaneous measurements of wall-pressure fluctuations and velocity measurements were performed by using microphone array and X-wire/split fim, respectively. 展开更多
关键词 ENTRAINMENT turbulent flow UNSTEADY wall pressure fluctuations large-scale vortical structures
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AN INTEGRATEDC METHOD FOR COMPUTING COMPUTING THE INTERNAL AND EXTERNAL VISCOUS FLOW FIELD AROUND THE DUCTED PROPULSOR BEHIND AN AXISYMMETRIC BODY
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作者 zhou lian-di Zhao Feng(China Ship Scientific Research Center, P.O. Box 116, Wuxi 214082, P. R. China) 《Journal of Hydrodynamics》 SCIE EI CSCD 1995年第3期72-83,共12页
This paper presents an effective method for computing the internal and external viscous flow field around the ducted propulsor behind an axisymmetric they by using a new Navier-Stokes equations solver with primitive v... This paper presents an effective method for computing the internal and external viscous flow field around the ducted propulsor behind an axisymmetric they by using a new Navier-Stokes equations solver with primitive variable continuity equation formulation. In the present numerical method, the calculation equation for pressure with well-defined coefficient,which form is similar to the artificial compressibility method, is developed. A semi-staggered grid system is adopted. Not only the advantage of staggered grid system can be retained but the boundary conditions on the inner and outer surface of the duct can be also carried out easily. By using a special grid system and the programming technique for implementing the jump boundary condition on the duct surfaces, the internal and external viscous flow field around the ducted propulsor behind the axisymmetric they may be calculated integrally in an unified numbered grid system. Some configurations are calculated and compared with experimental date and numerical results of other methods. Illustrative calculations are also presented for a stern of axisymmetric body with the backstep fitted a duct to illustrate the capability of the present method. Beside that, the effect of axial distribution of they force is considered and discussed in order to extend the application range of the present method. 展开更多
关键词 ducted propulsor viscous flow semi-staggered grid
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