目的:提出一种肩峰撞击理论以及肩关节Rockwood位片的肩峰形态分型法,探讨该分型法在肩袖损伤诊治中的应用价值。方法:回顾性分析2017年1月至2017年12月因肩峰撞击症或肩袖撕裂行肩关节镜手术的101例患者,其中男34例,女67例;年龄34~76(5...目的:提出一种肩峰撞击理论以及肩关节Rockwood位片的肩峰形态分型法,探讨该分型法在肩袖损伤诊治中的应用价值。方法:回顾性分析2017年1月至2017年12月因肩峰撞击症或肩袖撕裂行肩关节镜手术的101例患者,其中男34例,女67例;年龄34~76(56.31±9.63)岁;病程2~12个月,平均6个月。术前常规拍摄肩关节“三位片”,包括肩关节前后位、Rockwood位以及冈上肌Outlet位。基于肩峰撞击理论以及Rockwood位片将肩峰形态分为3型:Ⅰ型(平坦型)、Ⅱ型(隆起型)、Ⅲ型(撞击型)。两位观察者分别对101例患者的肩关节Rockwood位片按照新分型法、对冈上肌Outlet位片按照传统肩峰形态分型法进行分类统计;冈上肌腱损伤依据关节镜下所见分为无撕裂、部分撕裂、全层撕裂。分别对新分型法、传统分型法进行一致性检验(Kappa值),采用秩和检验比较新肩峰分型法3种肩峰形态的平均肩肱距(acromiohumeral distance,AHD),采用Spearman秩相关性检验以及Gamma法分析比较新肩峰分型法与冈上肌腱撕裂程度之间的相关性。结果:观察者间一致性检验新肩峰分型法优于传统肩峰分型法(0.827vs 0.278),观察者内一致性检验新肩峰分型法均优于传统肩峰分型法(0.921 vs 0.448,0.890 vs 0.539)。新的肩峰形态分型法3种肩峰形态的AHD比较差异无统计学意义(H=2.186,P>0.05)。所有101例患者撞击型肩峰占比最高为45.5%,隆起型肩峰次之36.6%,平坦型肩峰占17.8%。其中撞击型肩峰患者冈上肌腱撕裂发生率为95.7%,新的肩峰分型法与冈上肌腱撕裂程度存在Spearman秩相关(rs=0.719,P<0.001)。结论:肩关节Rockwood位片可以很好的显示肩峰前外侧骨赘,基于Rockwood位片新肩峰分型法可信度高,可重复性良好,其中撞击型肩峰与冈上肌腱撕裂存在密切关联。新分型法相比传统分型以及AHD对肩袖损伤更具诊疗价值。展开更多
目的:探讨关节镜治疗髋关节周围钙化性肌腱炎的可行性以及临床疗效。方法:回顾分析2013年5月至2018年7月采用关节镜手术治疗髋关节周围钙化性肌腱炎16例患者的病例资料,男10例,女6例,年龄35~63(44.50±6.67)岁,左髋9例,右髋7例,病程...目的:探讨关节镜治疗髋关节周围钙化性肌腱炎的可行性以及临床疗效。方法:回顾分析2013年5月至2018年7月采用关节镜手术治疗髋关节周围钙化性肌腱炎16例患者的病例资料,男10例,女6例,年龄35~63(44.50±6.67)岁,左髋9例,右髋7例,病程1~8(3.18±1.97) d。术前、术后1 d及末次随访采用疼痛视觉模拟评分(visual analogue scale,VAS),改良髋关节Harris评分(Harris hip scores,HHS),髋关节非骨性关节炎评分(nonarthritic hip score,NAHS)以及影像学进行髋关节功能评估。结果:所有患者顺利完成关节镜下髋关节周围钙化灶清除术,手术时间0.5~1.2(0.75±0.21) h。患者术后切口均Ⅰ期愈合,无感染、血肿以及血管神经损伤等并发症。所有患者获得随访,时间6~12(9.6±2.3)个月。术前患者VAS评分(7.88±0.72)分,改良HHS评分(29.25±3.23)分,NAHS评分(27.42±3.08)分,术后第1天患者髋关节疼痛以及活动度均明显改善,VAS评分(2.19±0.66)分,改良HHS评分(82.56±5.64)分,NAHS评分(82.11±2.94)分,与术前比较差异均有统计学意义(P<0.05)。末次随访所有患者影像学检查显示钙化灶完全消失,未见髋关节钙化灶复发,仅1例患者诉髋关节稍有酸胀感,VAS评分(0.38±0.50)分,改良HHS评分(94.31±2.82)分,NAHS评分(94.84±2.85)分,与术前比较差异均有统计学意义(P<0.05)。结论:采用关节镜治疗髋关节周围钙化性肌腱炎创伤小,可快速缓解疼痛改善髋关节功能,疗效确切。展开更多
In vitro gynogenesis is an important tool used in haploid or homozygous double-haploid plant breeding.However,because of low repeatability,embryoid induction rate and quality,the molecular mechanisms remain poorly und...In vitro gynogenesis is an important tool used in haploid or homozygous double-haploid plant breeding.However,because of low repeatability,embryoid induction rate and quality,the molecular mechanisms remain poorly understood.Heat shock treatment can promote the transformation of the gametophytic pathway into the sporophyte pathway,which induces the occurrence of haploid.In this study,unfertilized ovaries were heat shocked for 0 h(A0)before flowering and for 0 h(A1),4 h(A3),8 h(A5),12 h(A7),and 24 h(A8),respectively,at 37℃at the first day of the flowering stage.The ovule enlargement rate was increased from 0%at 25℃to 96.8%at 37℃(24 h treatment).Thus,we aimed to investigate the gene expression patterns in unfertilized ovules of watermelon after different periods of heat shock by using RNA-Seq technology.The results showed that compared with A3,A5,A7,and A8,the biosynthesis of amino acid,glycine,serine and threonine metabolic pathways in A1 has changed significantly.This indicated that heat shock treatment affected the synthesis and transformation of amino acids during ovule expansion.The transcriptome data suggested gene expressions of ovule growth were significantly changed by heat-specific influences.The results provide new information on the complex relationship between in vitro gynogenesis and temperature.This provides a basis for further study of the mechanism of heat shock affecting the expansion of watermelon ovule.展开更多
Watermelon(Citrullus lanatus(Thunb.) Matsum. & Nakai) is an important cucurbit crop grown worldwide. Watermelon fruit quality, fertility, and seed-setting rate are closely related to male flower development. In th...Watermelon(Citrullus lanatus(Thunb.) Matsum. & Nakai) is an important cucurbit crop grown worldwide. Watermelon fruit quality, fertility, and seed-setting rate are closely related to male flower development. In this study, the different developmental stages of flower buds of the watermelon cultivar ’Xinteda Zhengkang 9’ were distinguished by cytological observation, and transcriptome sequencing analysis was performed subsequently. Acetocarmine staining of anthers was performed and the longitudinal and transverse diameters of the unopened male flower buds were measured. Cytological observations of anthers at different developmental stages showed that the anther grew from the tetrad to the mature stage, and the longitudinal and transverse diameters of the flower buds increased. The length of the male flower buds also changed significantly during development. Transcriptome sequencing analysis at four periods, the tetrad(A group), mononuclear(B group), dikaryophase(C group), and mature stages(D group). A total of 16 288 differentially expressed genes(DEGs) were detected in the four stages, with the prolongation of developmental stages, the number of DEGs increased gradually in the comparison groups, there was 2 014, 3 259, 4 628, 1 490, 3 495 and 1 132 DEGs revealed in six comparison groups(A-vs.-B, A-vs.-C, A-vs.-D, B-vs.-C, B-vs.-D, and C-vs.-D), respectively. Gene Ontology(GO) and KEGG enrichment analysis showed that the DEGs were mainly enriched in cellular component and starch and sucrose metabolism, phenylpropanoid biosynthesis and pentose sugar, etc. Finally, we completely screened 59 DEGs in the six comparison groups, interestingly, we found one pollen-specific protein(Cla001608) that was significantly down-regulated(the value of log2 Fold Change up to 17.32), which indicated that it may play an important role in the development of male flowers. This work provides insight into the molecular basis of the developmental stages of male flowers in watermelon and may aid in dominant cross breeding.展开更多
文摘目的:提出一种肩峰撞击理论以及肩关节Rockwood位片的肩峰形态分型法,探讨该分型法在肩袖损伤诊治中的应用价值。方法:回顾性分析2017年1月至2017年12月因肩峰撞击症或肩袖撕裂行肩关节镜手术的101例患者,其中男34例,女67例;年龄34~76(56.31±9.63)岁;病程2~12个月,平均6个月。术前常规拍摄肩关节“三位片”,包括肩关节前后位、Rockwood位以及冈上肌Outlet位。基于肩峰撞击理论以及Rockwood位片将肩峰形态分为3型:Ⅰ型(平坦型)、Ⅱ型(隆起型)、Ⅲ型(撞击型)。两位观察者分别对101例患者的肩关节Rockwood位片按照新分型法、对冈上肌Outlet位片按照传统肩峰形态分型法进行分类统计;冈上肌腱损伤依据关节镜下所见分为无撕裂、部分撕裂、全层撕裂。分别对新分型法、传统分型法进行一致性检验(Kappa值),采用秩和检验比较新肩峰分型法3种肩峰形态的平均肩肱距(acromiohumeral distance,AHD),采用Spearman秩相关性检验以及Gamma法分析比较新肩峰分型法与冈上肌腱撕裂程度之间的相关性。结果:观察者间一致性检验新肩峰分型法优于传统肩峰分型法(0.827vs 0.278),观察者内一致性检验新肩峰分型法均优于传统肩峰分型法(0.921 vs 0.448,0.890 vs 0.539)。新的肩峰形态分型法3种肩峰形态的AHD比较差异无统计学意义(H=2.186,P>0.05)。所有101例患者撞击型肩峰占比最高为45.5%,隆起型肩峰次之36.6%,平坦型肩峰占17.8%。其中撞击型肩峰患者冈上肌腱撕裂发生率为95.7%,新的肩峰分型法与冈上肌腱撕裂程度存在Spearman秩相关(rs=0.719,P<0.001)。结论:肩关节Rockwood位片可以很好的显示肩峰前外侧骨赘,基于Rockwood位片新肩峰分型法可信度高,可重复性良好,其中撞击型肩峰与冈上肌腱撕裂存在密切关联。新分型法相比传统分型以及AHD对肩袖损伤更具诊疗价值。
文摘目的:探讨关节镜治疗髋关节周围钙化性肌腱炎的可行性以及临床疗效。方法:回顾分析2013年5月至2018年7月采用关节镜手术治疗髋关节周围钙化性肌腱炎16例患者的病例资料,男10例,女6例,年龄35~63(44.50±6.67)岁,左髋9例,右髋7例,病程1~8(3.18±1.97) d。术前、术后1 d及末次随访采用疼痛视觉模拟评分(visual analogue scale,VAS),改良髋关节Harris评分(Harris hip scores,HHS),髋关节非骨性关节炎评分(nonarthritic hip score,NAHS)以及影像学进行髋关节功能评估。结果:所有患者顺利完成关节镜下髋关节周围钙化灶清除术,手术时间0.5~1.2(0.75±0.21) h。患者术后切口均Ⅰ期愈合,无感染、血肿以及血管神经损伤等并发症。所有患者获得随访,时间6~12(9.6±2.3)个月。术前患者VAS评分(7.88±0.72)分,改良HHS评分(29.25±3.23)分,NAHS评分(27.42±3.08)分,术后第1天患者髋关节疼痛以及活动度均明显改善,VAS评分(2.19±0.66)分,改良HHS评分(82.56±5.64)分,NAHS评分(82.11±2.94)分,与术前比较差异均有统计学意义(P<0.05)。末次随访所有患者影像学检查显示钙化灶完全消失,未见髋关节钙化灶复发,仅1例患者诉髋关节稍有酸胀感,VAS评分(0.38±0.50)分,改良HHS评分(94.31±2.82)分,NAHS评分(94.84±2.85)分,与术前比较差异均有统计学意义(P<0.05)。结论:采用关节镜治疗髋关节周围钙化性肌腱炎创伤小,可快速缓解疼痛改善髋关节功能,疗效确切。
基金supported by the earmarked fund for China Agriculture Research System (CARS-25)the Fundamental Research Funds of the Chinese Academy of Agricultural Sciences (Y2018YJ15 and Y2019XK16-03)+1 种基金the Agricultural Science and Technology Innovation Program, Chinese Academy of Agricultural Sciences (CAAS-ASTIP2018-ZFRI)the National Key R&D Program of China (2018YFD0201310)
文摘In vitro gynogenesis is an important tool used in haploid or homozygous double-haploid plant breeding.However,because of low repeatability,embryoid induction rate and quality,the molecular mechanisms remain poorly understood.Heat shock treatment can promote the transformation of the gametophytic pathway into the sporophyte pathway,which induces the occurrence of haploid.In this study,unfertilized ovaries were heat shocked for 0 h(A0)before flowering and for 0 h(A1),4 h(A3),8 h(A5),12 h(A7),and 24 h(A8),respectively,at 37℃at the first day of the flowering stage.The ovule enlargement rate was increased from 0%at 25℃to 96.8%at 37℃(24 h treatment).Thus,we aimed to investigate the gene expression patterns in unfertilized ovules of watermelon after different periods of heat shock by using RNA-Seq technology.The results showed that compared with A3,A5,A7,and A8,the biosynthesis of amino acid,glycine,serine and threonine metabolic pathways in A1 has changed significantly.This indicated that heat shock treatment affected the synthesis and transformation of amino acids during ovule expansion.The transcriptome data suggested gene expressions of ovule growth were significantly changed by heat-specific influences.The results provide new information on the complex relationship between in vitro gynogenesis and temperature.This provides a basis for further study of the mechanism of heat shock affecting the expansion of watermelon ovule.
基金supported by the China Agriculture Research System of MOF and MARA(CARS-25)the Central Public-interest Scientific Institution Basal Research Fund,China(Y2019XK16-03)+2 种基金the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(CAASASTIP-2020-ZFRI)the Key Scientific and Technological Project of Henan Province,China(202102110194)the Major Science and Technology Project in Zhengzhou,China(188PCXZX802)。
文摘Watermelon(Citrullus lanatus(Thunb.) Matsum. & Nakai) is an important cucurbit crop grown worldwide. Watermelon fruit quality, fertility, and seed-setting rate are closely related to male flower development. In this study, the different developmental stages of flower buds of the watermelon cultivar ’Xinteda Zhengkang 9’ were distinguished by cytological observation, and transcriptome sequencing analysis was performed subsequently. Acetocarmine staining of anthers was performed and the longitudinal and transverse diameters of the unopened male flower buds were measured. Cytological observations of anthers at different developmental stages showed that the anther grew from the tetrad to the mature stage, and the longitudinal and transverse diameters of the flower buds increased. The length of the male flower buds also changed significantly during development. Transcriptome sequencing analysis at four periods, the tetrad(A group), mononuclear(B group), dikaryophase(C group), and mature stages(D group). A total of 16 288 differentially expressed genes(DEGs) were detected in the four stages, with the prolongation of developmental stages, the number of DEGs increased gradually in the comparison groups, there was 2 014, 3 259, 4 628, 1 490, 3 495 and 1 132 DEGs revealed in six comparison groups(A-vs.-B, A-vs.-C, A-vs.-D, B-vs.-C, B-vs.-D, and C-vs.-D), respectively. Gene Ontology(GO) and KEGG enrichment analysis showed that the DEGs were mainly enriched in cellular component and starch and sucrose metabolism, phenylpropanoid biosynthesis and pentose sugar, etc. Finally, we completely screened 59 DEGs in the six comparison groups, interestingly, we found one pollen-specific protein(Cla001608) that was significantly down-regulated(the value of log2 Fold Change up to 17.32), which indicated that it may play an important role in the development of male flowers. This work provides insight into the molecular basis of the developmental stages of male flowers in watermelon and may aid in dominant cross breeding.
