The purpose of this study is to construct OPA1 overexpression plasmid and transfected into human neuroblastoma SH-SY5Y cells to test the overexpression efficiency.Human neuroblastoma SH SY5Y cells were transfected wit...The purpose of this study is to construct OPA1 overexpression plasmid and transfected into human neuroblastoma SH-SY5Y cells to test the overexpression efficiency.Human neuroblastoma SH SY5Y cells were transfected with the OPA1 overexpression plasmid by Lipo3000 transfection reagent in liposome transduction technology.A total of 3 groups were set up:the control group,negative control group,and OPA1 overexpression model group.RT-PCR technique was used to detect the expression of OPA1 at mRNA level.In addition,Western blot technique was used to detect the expression of OPA1 at protein level.We found that the mRNA and protein expression of OPA1 were significantly increased after transfection with OPA1 overexpression plasmid.These results highlight using liposome transduction technology,the OPA1 overexpression plasmid was successfully transfected into SH-SY5Y cells by Lipo3000 and the cell model of OPA1 gene overexpression was successully established.展开更多
Explore the effect of Timosaponin B-Ⅱ on morphology and function of mitochondria in Parkinson's disease(PD)cell model,and lay the foundation for screening and mechanism research of anti-Parkinson's disease dr...Explore the effect of Timosaponin B-Ⅱ on morphology and function of mitochondria in Parkinson's disease(PD)cell model,and lay the foundation for screening and mechanism research of anti-Parkinson's disease drugs.The PD cell model was constructed by applying MPP+to SH-SY5Y cells for 48 h,and they were treated with low,.medium and high concentrations of Timosaponin B-Ⅱ.展开更多
This study was performed to investigate the effect and mechanism of Buyang Huanwu Decoction(BYHWD)and its disassembled prescriptions on nerve function and angiogenesis in ischemic stroke rats with Qi deficiency and bl...This study was performed to investigate the effect and mechanism of Buyang Huanwu Decoction(BYHWD)and its disassembled prescriptions on nerve function and angiogenesis in ischemic stroke rats with Qi deficiency and blood stasis syndrome.Rats were randomly divided into six groups:Sham group,Model group,BYHWD group,Qi-tonifying group,Blood-activating group and Butyphthalide group.Rats were used to make permanent middle cerebral artery occlusion(MCAO)after 10 days of simulated intervention with compound factors of Qi deficiency and blood stasis syndrome,followed by the medicine group was administered intragastrically for a week.展开更多
The perineuronal nets(PNNs)are a kind of extracellular matrix structure which mainly surround parvalbumin-positive(PV^(+))GABAergic interneurons.The aim of this study is to investigate the role of PNNs in the medial p...The perineuronal nets(PNNs)are a kind of extracellular matrix structure which mainly surround parvalbumin-positive(PV^(+))GABAergic interneurons.The aim of this study is to investigate the role of PNNs in the medial prefrontal cortex(mPFC)in the antidepressant effect of electroacupuncture treatment.Adult rats underwent chronic unpredictable mild stress(CUMS)for 28 days,and electroacupuncture treatment of Baihui and Yintang acupoints was administrated from day 8 to day 28.Then we detected the depressive-and anxiety like behavior of rats,the density of PNNs,the percentage of PV^(+)neurons that surround PNNs,the expression levels of PNNs constituting proteins in the mPFC.展开更多
文摘The purpose of this study is to construct OPA1 overexpression plasmid and transfected into human neuroblastoma SH-SY5Y cells to test the overexpression efficiency.Human neuroblastoma SH SY5Y cells were transfected with the OPA1 overexpression plasmid by Lipo3000 transfection reagent in liposome transduction technology.A total of 3 groups were set up:the control group,negative control group,and OPA1 overexpression model group.RT-PCR technique was used to detect the expression of OPA1 at mRNA level.In addition,Western blot technique was used to detect the expression of OPA1 at protein level.We found that the mRNA and protein expression of OPA1 were significantly increased after transfection with OPA1 overexpression plasmid.These results highlight using liposome transduction technology,the OPA1 overexpression plasmid was successfully transfected into SH-SY5Y cells by Lipo3000 and the cell model of OPA1 gene overexpression was successully established.
文摘Explore the effect of Timosaponin B-Ⅱ on morphology and function of mitochondria in Parkinson's disease(PD)cell model,and lay the foundation for screening and mechanism research of anti-Parkinson's disease drugs.The PD cell model was constructed by applying MPP+to SH-SY5Y cells for 48 h,and they were treated with low,.medium and high concentrations of Timosaponin B-Ⅱ.
文摘This study was performed to investigate the effect and mechanism of Buyang Huanwu Decoction(BYHWD)and its disassembled prescriptions on nerve function and angiogenesis in ischemic stroke rats with Qi deficiency and blood stasis syndrome.Rats were randomly divided into six groups:Sham group,Model group,BYHWD group,Qi-tonifying group,Blood-activating group and Butyphthalide group.Rats were used to make permanent middle cerebral artery occlusion(MCAO)after 10 days of simulated intervention with compound factors of Qi deficiency and blood stasis syndrome,followed by the medicine group was administered intragastrically for a week.
文摘The perineuronal nets(PNNs)are a kind of extracellular matrix structure which mainly surround parvalbumin-positive(PV^(+))GABAergic interneurons.The aim of this study is to investigate the role of PNNs in the medial prefrontal cortex(mPFC)in the antidepressant effect of electroacupuncture treatment.Adult rats underwent chronic unpredictable mild stress(CUMS)for 28 days,and electroacupuncture treatment of Baihui and Yintang acupoints was administrated from day 8 to day 28.Then we detected the depressive-and anxiety like behavior of rats,the density of PNNs,the percentage of PV^(+)neurons that surround PNNs,the expression levels of PNNs constituting proteins in the mPFC.
