Objective: To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum pro-teomics technique, and to screen the different expression proteins related with psoriasis vulg...Objective: To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum pro-teomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods: Serum samples ofperipheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons.Serum albumin IgG was removed by filtering with ProteoExtract Albumin /IgG. After comparative proteomics analysis the dif-ferent protein spots were identified using 2-DE and MS. Results: Electrophoresis figures with high resolution and repro-ducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients,while nine other different proteins expressing from healthy volunteers. Conclusion: The protein expression was different in theserum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related topsoriasis vulgaris by using proteomics.展开更多
文摘Objective: To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum pro-teomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods: Serum samples ofperipheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons.Serum albumin IgG was removed by filtering with ProteoExtract Albumin /IgG. After comparative proteomics analysis the dif-ferent protein spots were identified using 2-DE and MS. Results: Electrophoresis figures with high resolution and repro-ducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients,while nine other different proteins expressing from healthy volunteers. Conclusion: The protein expression was different in theserum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related topsoriasis vulgaris by using proteomics.
