This study was conducted to explore the multiplication pattern of the recombinant strain Re-7 of avian influenza virus subtype H5 in Madin Darby Canine Kidney （MDCK） cells and to determine the optimal multiplicity o...This study was conducted to explore the multiplication pattern of the recombinant strain Re-7 of avian influenza virus subtype H5 in Madin Darby Canine Kidney （MDCK） cells and to determine the optimal multiplicity of infection （MOI） and the optimal time for virus harvest. The recombinant strain Re-7 was inoculated at different MOIs into MDCK cells grown in serum-free medium in 100 L bioreactors for replication. Then, the hemagglutination（HA） titer, 50% tissue culture infectious dose （TCID50） and 50% embryo infectious dose （EID50） of culture medium were measured once every 12 h from 24 h after virus inoculation to determine the optimal MOI. After that, virus was inoculated at the optimal MOI determined above into MDCK cells for large-scale virus replication to determine the optimal time for virus harvest. The results showed that the optimal MOI was 10 2, and the optimal time for virus harvest was 60 h after inoculation. Under these conditions, the HA titer, TCIDso per 1 mL and EIDso per 0.1 mL were increased to 1：102 4, 10^7.33 and 10^6.83, respectively. This study provides relatively stable parameters for large-scale production of the recombinant strain Re-7 of avian influenza virus subtype H5.展开更多
目的分析2012—2017年北京某综合医院人类免疫缺陷病毒(human immunodeficiency virus,HIV)抗体检测阳性者的流行病学特点及梅毒感染情况,为区域性HIV筛查及防治提供依据。方法对2012—2017年北京某综合医院门诊与住院患者中经HIV抗体...目的分析2012—2017年北京某综合医院人类免疫缺陷病毒(human immunodeficiency virus,HIV)抗体检测阳性者的流行病学特点及梅毒感染情况,为区域性HIV筛查及防治提供依据。方法对2012—2017年北京某综合医院门诊与住院患者中经HIV抗体初筛和确认的HIV感染者相关数据进行描述性流行病学分析。结果 2012—2017年共进行HIV筛查222 550人次,初筛阳性285例(0.13%),确证阳性246例(0.11%)。各年HIV阳性率较稳定,上升趋势不明显。HIV抗体确认阳性者以男性为主,占96.34%,男女比例26.33∶1,年龄上以20～39岁年龄段为主(82.52%),外省市户籍者(84.96%)居多,婚姻状况以已婚为主(56.50%),且已婚比例随时间有升高趋势(P<0.05);传播途径上均为性传播,以男男同性性传播为主(96.34%)。就诊科室主要分布在皮肤科(43.50%)、内科(16.67%)、外科(13.41%)和体检中心(11.79%),在整个内科来源的HIV阳性者中,消化内科占的比例为65.85%。HIV阳性者合并感染梅毒的几率很大(26.02%),且梅毒发作期患者比例高(20.33%)。结论需进一步加强对大型综合性医院就诊患者的HIV检测。性活跃期MSM(men who have sex with men)人群(尤其是双性性接触的MSM)需继续作为艾滋病防控宣传和咨询检测的重点人群,防止HIV感染由高危人群向一般人群传播。及时治疗梅毒感染,有效控制梅毒的发病率,对于控制艾滋病的流行有着重要意义。展开更多
文摘This study was conducted to explore the multiplication pattern of the recombinant strain Re-7 of avian influenza virus subtype H5 in Madin Darby Canine Kidney （MDCK） cells and to determine the optimal multiplicity of infection （MOI） and the optimal time for virus harvest. The recombinant strain Re-7 was inoculated at different MOIs into MDCK cells grown in serum-free medium in 100 L bioreactors for replication. Then, the hemagglutination（HA） titer, 50% tissue culture infectious dose （TCID50） and 50% embryo infectious dose （EID50） of culture medium were measured once every 12 h from 24 h after virus inoculation to determine the optimal MOI. After that, virus was inoculated at the optimal MOI determined above into MDCK cells for large-scale virus replication to determine the optimal time for virus harvest. The results showed that the optimal MOI was 10 2, and the optimal time for virus harvest was 60 h after inoculation. Under these conditions, the HA titer, TCIDso per 1 mL and EIDso per 0.1 mL were increased to 1：102 4, 10^7.33 and 10^6.83, respectively. This study provides relatively stable parameters for large-scale production of the recombinant strain Re-7 of avian influenza virus subtype H5.
文摘目的分析2012—2017年北京某综合医院人类免疫缺陷病毒(human immunodeficiency virus,HIV)抗体检测阳性者的流行病学特点及梅毒感染情况,为区域性HIV筛查及防治提供依据。方法对2012—2017年北京某综合医院门诊与住院患者中经HIV抗体初筛和确认的HIV感染者相关数据进行描述性流行病学分析。结果 2012—2017年共进行HIV筛查222 550人次,初筛阳性285例(0.13%),确证阳性246例(0.11%)。各年HIV阳性率较稳定,上升趋势不明显。HIV抗体确认阳性者以男性为主,占96.34%,男女比例26.33∶1,年龄上以20～39岁年龄段为主(82.52%),外省市户籍者(84.96%)居多,婚姻状况以已婚为主(56.50%),且已婚比例随时间有升高趋势(P<0.05);传播途径上均为性传播,以男男同性性传播为主(96.34%)。就诊科室主要分布在皮肤科(43.50%)、内科(16.67%)、外科(13.41%)和体检中心(11.79%),在整个内科来源的HIV阳性者中,消化内科占的比例为65.85%。HIV阳性者合并感染梅毒的几率很大(26.02%),且梅毒发作期患者比例高(20.33%)。结论需进一步加强对大型综合性医院就诊患者的HIV检测。性活跃期MSM(men who have sex with men)人群(尤其是双性性接触的MSM)需继续作为艾滋病防控宣传和咨询检测的重点人群,防止HIV感染由高危人群向一般人群传播。及时治疗梅毒感染,有效控制梅毒的发病率,对于控制艾滋病的流行有着重要意义。
