本研究通过观察2微米激光犬前列腺切除术（two-micronlaser resection of the prostate，TmLRP）后前列腺部尿道创伤修复病理组织学改变，探讨前列腺部尿道创面再上皮化方式并评估该再上皮化方式的效果。选取15只成年健康雄性中华田园...本研究通过观察2微米激光犬前列腺切除术（two-micronlaser resection of the prostate，TmLRP）后前列腺部尿道创伤修复病理组织学改变，探讨前列腺部尿道创面再上皮化方式并评估该再上皮化方式的效果。选取15只成年健康雄性中华田园犬，分别行2微米激光前列腺汽化切除术及部分膀胱颈黏膜切除术，于术后3天、1、2、3及4周处死动物，留取前列腺部尿道及膀胱颈标本，苏木素伊红染色（Hematoxylin eosin，HE）染色光镜下观察创面再上皮化病理组织学改变，免疫组织化学染色检测前列腺部尿道创面细胞角蛋白14（Cytokeratin，CK14）、CK5、 CK18 , 突触素（Synaptophysin，Syn）、嗜铬素（Chromogranin A , CgA）、尿斑蛋白（uroplakin）、转化生长因子β1 （Transforming growth factor-β1, TGF-β1）和转化生长因子βII受体（Transforming growth factor-β type II receptor,TGF-βRI I）的表达，实时定量PCR方法检测前列腺部尿道创面CK14、CK5、CK18和Syn mRNA表达，Van Gieson染色分别检测前列腺部尿道和膀胱颈创面胶原纤维表达量。结果显示，前列腺部尿道由创面下残余前列腺上皮细胞增殖、迁移再分化完成再上皮化过程；创面下增殖的前列腺上皮细胞表达CK14，CK5，不表达CK18、Sy n和CgA，再生上皮术后3周开始表达uroplakin。增殖的前列腺细胞和再生上皮高表达TGF-β1和TGF-β RII，表达强度与创面修复再上皮化时间密切相关。与膀胱颈创面相比，前列腺部尿道创面再上皮化完成快，创面下胶原纤维形成少。因此，源自创面下前列腺基底细胞的再上皮化方式可能是机体实现由解剖修复到功能修复的最佳修复方式。展开更多
We conducted the present study to assess the correlation of the prostatic anatomical parameters,especially the ratio of peripheral zone thickness and transitional zone thickness,with clinical and uroflowmetry characte...We conducted the present study to assess the correlation of the prostatic anatomical parameters,especially the ratio of peripheral zone thickness and transitional zone thickness,with clinical and uroflowmetry characteristics suggestive of benign prostate hyperplasia(BPH).A total of 468 consecutive patients with a detailed medical history were identified.All patients were evaluated by scoring subjective symptoms with the International Prostate Symptom Score(IPSS)and quality of life(QoL).The prostatic anatomical parameters were measured using transrectal ultrasonography,and postvoid residual urine and maximum flow rate(Q_(max))values were also determined.Pearsonfs correlation analysis revealed that both total prostate volume(TPV;r=0.160,P<0.001)and transitional zone volume(TZV;r=0.104,P=0.016)increased with patients7 age;however,no correlations were observed of TPV,TZV,transitional zone index(TZI),and transitional zone thickness(TZT)with IPSS or QoL(all P>0.05).Peripheral to transitional zone index(PTI)was found negatively correlated with total IPSS(r=-0.113,P=0.024),storage IPSS(r=-0.103,P=0.041),and voiding IPSS(r=-0.123,P-0.014).As regards the uroflowmetry characteristics,PTI(r=0.157,P=0.007)was indicated to be positively correlated with Q_(max)and negatively correlated with TZI(r=-0.119,P=0.042)and TZT(r=-0.118,P=0.045),but not correlated with TPV,TZV,or peripheral zone thickness(PZT)(all P>0.05).Postvoid residual urine(PVR)had not correlated with all the prostatic anatomical variables(all P>0.05).This is the first study that formally proposed the concept of PTI,which is an easy-to-measure prostate anatomical parameter which significantly correlates with total IPSS,storage IPSS,voiding IPSS,and Q_(max),suggesting that PTI would be useful in evaluating and managing men with lower urinary tract symptoms(LUTS)/BPH.However,well-designed studies are mandatory to verify the clinical utility of PTI.展开更多
基金ACKNOWLEDGMENTS This study was supported by the Natural Science Foundation of China （81260119） and the Guizhou Science and Technology Agency Fund （[201214013 SZ201213077]+1 种基金 SY2011 [3024] LS2011 [020]）.
基金This study was supported by grants from the research program of National Science Foundation of China(No.81860141)Health and Family Planning Commission of Guizhou Province Foundation(No.gzwjkj2017-1-032)Doctoral Foundation of Guizhou Provincial Peoples Hospital(No.GZSYBS[2016]11).
文摘We conducted the present study to assess the correlation of the prostatic anatomical parameters,especially the ratio of peripheral zone thickness and transitional zone thickness,with clinical and uroflowmetry characteristics suggestive of benign prostate hyperplasia(BPH).A total of 468 consecutive patients with a detailed medical history were identified.All patients were evaluated by scoring subjective symptoms with the International Prostate Symptom Score(IPSS)and quality of life(QoL).The prostatic anatomical parameters were measured using transrectal ultrasonography,and postvoid residual urine and maximum flow rate(Q_(max))values were also determined.Pearsonfs correlation analysis revealed that both total prostate volume(TPV;r=0.160,P<0.001)and transitional zone volume(TZV;r=0.104,P=0.016)increased with patients7 age;however,no correlations were observed of TPV,TZV,transitional zone index(TZI),and transitional zone thickness(TZT)with IPSS or QoL(all P>0.05).Peripheral to transitional zone index(PTI)was found negatively correlated with total IPSS(r=-0.113,P=0.024),storage IPSS(r=-0.103,P=0.041),and voiding IPSS(r=-0.123,P-0.014).As regards the uroflowmetry characteristics,PTI(r=0.157,P=0.007)was indicated to be positively correlated with Q_(max)and negatively correlated with TZI(r=-0.119,P=0.042)and TZT(r=-0.118,P=0.045),but not correlated with TPV,TZV,or peripheral zone thickness(PZT)(all P>0.05).Postvoid residual urine(PVR)had not correlated with all the prostatic anatomical variables(all P>0.05).This is the first study that formally proposed the concept of PTI,which is an easy-to-measure prostate anatomical parameter which significantly correlates with total IPSS,storage IPSS,voiding IPSS,and Q_(max),suggesting that PTI would be useful in evaluating and managing men with lower urinary tract symptoms(LUTS)/BPH.However,well-designed studies are mandatory to verify the clinical utility of PTI.
