The sustainable recovery and utilization of sludge bioenergy within a circular economy context has drawn increasing attention,but there is currently a shortage of reliable technology.This study presents an innovative ...The sustainable recovery and utilization of sludge bioenergy within a circular economy context has drawn increasing attention,but there is currently a shortage of reliable technology.This study presents an innovative biotechnology based on free nitrous acid(FNA)to realize sustainable organics recovery from waste activated sludge(WAS)in-situ,driving efficient nitrogen removal from ammonia rich mature landfill leachate by integrating partial nitrification,fermentation,and denitrification process(PN/DN-F/DN).First,ammonia((1708.5±142.9)mg·L^(-1))in mature landfill leachate is oxidized to nitrite in the aerobic stage of a partial nitrification coupling denitrification(PN/DN)sequencing batch reactor(SBR),with nitrite accumulation ratio of 95.4%±2.5%.Then,intermediate effluent(NO_(2)^(-)-N=(1196.9±184.2)mg·L^(-1))of the PN/DN-SBR,along with concentrated WAS(volatile solids(VSs)=(15119.8±2484.2)mg·L^(-1)),is fed into an anoxic reactor for fermentation coupling denitrification process(F/DN).FNA,the protonated form of nitrite,degrades organics in the WAS to the soluble fraction by the biocidal effect,and the released organics are utilized by denitrifiers to drive NOx-reduction.An ultra-fast sludge reduction rate of 4.89 kg·m^(-3)·d^(-1) and nitrogen removal rate of 0.46 kg·m^(-3)·d^(-1) were realized in the process.Finally,F/DN-SBR effluent containing organics is refluxed to PN/DN-SBR for secondary denitrification in the post anoxic stage.After 175 d operation,an average of 19350.6 mg chemical oxygen demand organics were recovered per operational cycle,with 95.2%nitrogen removal and 53.4%sludge reduction.PN/DN-F/DN is of great significance for promoting a paradigm transformation from energy consumption to energy neutral,specifically,the total benefit in equivalent terms of energy was 291.8 kW·h·t^(-1) total solid.展开更多
Peganum harmala L.is a medicinal plant,and its seeds have been used to treat gastrointestinal cancer and malaria for a long time in North-Western China.In the present study,we aimed to probe the potential molecular ta...Peganum harmala L.is a medicinal plant,and its seeds have been used to treat gastrointestinal cancer and malaria for a long time in North-Western China.In the present study,we aimed to probe the potential molecular targets and pharmacological mechanisms of Peganum harmala L.seeds(PHS)on hepatocellular carcinoma(HCC)using network analysis and molecular docking.First,the chemical ingredients of PHS were obtained from TCMID and BATMAN-TCM databases,and the effective ingredients were screened by SwissADME.Furthermore,the target information of the effective ingredients was acquired from PharmMapper and SwissTargetPrediction databases.Secondly,HCC-related targets were obtained from Liverome,DisGeNET,and GeneCards databases.The intersection with the PHS was obtained.The“compounds-targets”was drawn using Cytoscape software,and PPI network diagrams were drawn using their intersection targets.GO analysis and KEGG enrichment analysis were carried out using the DAVID database.Finally,molecular docking was conducted between protein receptors and the active components using AutoDockTools.Our results showed 105 intersection targets of PHS with HCC.Moreover,10 core targets included ALB,AKT1,EGFR,CASP3,SRC,ESR1,MAPK3,MMP9,ANXA5,and MAPK1.Besides,404 GO functional annotations were obtained,including 287 biological processes,37 cell compositions,and 80 molecular functions.In addition,110 signaling molecules and pathways,including chemical oncogene receptors,PI3K-Akt pathway,HCC,and hepatitis B,were identified.The molecular docking results showed that the binding energies of the 10 core targets and the 12 active components were all less than–5 kcal/mol.In conclusion,this study expounded on the“component-target-pathway”interaction mechanism of PHS for the treatment of HCC,and it also provided a scientific basis for the clinical application of PHS.展开更多
A local algorithm is proposed for unconstrained optimization problem. Compared with the traditional Newton method with Choleski factorization, this algorithm has the same quadratic convergence. But its computation cos...A local algorithm is proposed for unconstrained optimization problem. Compared with the traditional Newton method with Choleski factorization, this algorithm has the same quadratic convergence. But its computation cost per iteration in average is less when the dimension n≥55. The saving is estimated in the theoretical framework.展开更多
基金supported by the Beijing Natural Science Foundation (8222040)Key Program of National Natural Science Foundation of China (52131004)+4 种基金Young Elite Scientists Sponsorship Program by China association for science and technology (CAST,YESS20220508)Young Elite Scientists Sponsorship Program by Beijing Association for Science and Technology (BAST,BYESS2023183)Innovation and Entrepreneurship Leading Team Project in Guangzhou (CYLJTD-201607)Key Research and Developmental Program of Shandong Province (2020CXGC011404)Cultivating Fund of Faculty of Environment and Life,Beijing University of Technology (PY202302).
文摘The sustainable recovery and utilization of sludge bioenergy within a circular economy context has drawn increasing attention,but there is currently a shortage of reliable technology.This study presents an innovative biotechnology based on free nitrous acid(FNA)to realize sustainable organics recovery from waste activated sludge(WAS)in-situ,driving efficient nitrogen removal from ammonia rich mature landfill leachate by integrating partial nitrification,fermentation,and denitrification process(PN/DN-F/DN).First,ammonia((1708.5±142.9)mg·L^(-1))in mature landfill leachate is oxidized to nitrite in the aerobic stage of a partial nitrification coupling denitrification(PN/DN)sequencing batch reactor(SBR),with nitrite accumulation ratio of 95.4%±2.5%.Then,intermediate effluent(NO_(2)^(-)-N=(1196.9±184.2)mg·L^(-1))of the PN/DN-SBR,along with concentrated WAS(volatile solids(VSs)=(15119.8±2484.2)mg·L^(-1)),is fed into an anoxic reactor for fermentation coupling denitrification process(F/DN).FNA,the protonated form of nitrite,degrades organics in the WAS to the soluble fraction by the biocidal effect,and the released organics are utilized by denitrifiers to drive NOx-reduction.An ultra-fast sludge reduction rate of 4.89 kg·m^(-3)·d^(-1) and nitrogen removal rate of 0.46 kg·m^(-3)·d^(-1) were realized in the process.Finally,F/DN-SBR effluent containing organics is refluxed to PN/DN-SBR for secondary denitrification in the post anoxic stage.After 175 d operation,an average of 19350.6 mg chemical oxygen demand organics were recovered per operational cycle,with 95.2%nitrogen removal and 53.4%sludge reduction.PN/DN-F/DN is of great significance for promoting a paradigm transformation from energy consumption to energy neutral,specifically,the total benefit in equivalent terms of energy was 291.8 kW·h·t^(-1) total solid.
基金The National Natural Science Foundation of China(Grant No.81760637,81460539)the National Natural Science Foundation of Xinjiang(Grant No.2017D01C207)+1 种基金the Open Research Fund of Xinjiang Uygur Autonomous Region Key Laboratory(Grant No.XJDX1713)。
文摘Peganum harmala L.is a medicinal plant,and its seeds have been used to treat gastrointestinal cancer and malaria for a long time in North-Western China.In the present study,we aimed to probe the potential molecular targets and pharmacological mechanisms of Peganum harmala L.seeds(PHS)on hepatocellular carcinoma(HCC)using network analysis and molecular docking.First,the chemical ingredients of PHS were obtained from TCMID and BATMAN-TCM databases,and the effective ingredients were screened by SwissADME.Furthermore,the target information of the effective ingredients was acquired from PharmMapper and SwissTargetPrediction databases.Secondly,HCC-related targets were obtained from Liverome,DisGeNET,and GeneCards databases.The intersection with the PHS was obtained.The“compounds-targets”was drawn using Cytoscape software,and PPI network diagrams were drawn using their intersection targets.GO analysis and KEGG enrichment analysis were carried out using the DAVID database.Finally,molecular docking was conducted between protein receptors and the active components using AutoDockTools.Our results showed 105 intersection targets of PHS with HCC.Moreover,10 core targets included ALB,AKT1,EGFR,CASP3,SRC,ESR1,MAPK3,MMP9,ANXA5,and MAPK1.Besides,404 GO functional annotations were obtained,including 287 biological processes,37 cell compositions,and 80 molecular functions.In addition,110 signaling molecules and pathways,including chemical oncogene receptors,PI3K-Akt pathway,HCC,and hepatitis B,were identified.The molecular docking results showed that the binding energies of the 10 core targets and the 12 active components were all less than–5 kcal/mol.In conclusion,this study expounded on the“component-target-pathway”interaction mechanism of PHS for the treatment of HCC,and it also provided a scientific basis for the clinical application of PHS.
文摘A local algorithm is proposed for unconstrained optimization problem. Compared with the traditional Newton method with Choleski factorization, this algorithm has the same quadratic convergence. But its computation cost per iteration in average is less when the dimension n≥55. The saving is estimated in the theoretical framework.
