Objective： To analyze the cause and pathogenesis of nerve injury and find out the suitable acupuncture points. Methods： Acupuncture at the unilateral points of Hegu （LI4）, Waiguan （TB5）, Shousanli （LI10）, ...Objective： To analyze the cause and pathogenesis of nerve injury and find out the suitable acupuncture points. Methods： Acupuncture at the unilateral points of Hegu （LI4）, Waiguan （TB5）, Shousanli （LI10）, Quchi （LI11） and the bilateral points of Zusanli （ST36）, accompanied by method of electro-acupuncture. Results： After three courses of acupuncture treatment （30 minutes each time, 6 times each course）, the patient presented the symptoms of making a fist strongly, disappearance of wrist drop, and positive sign of lifting wrist assay in turn. Simutaneously, the grip strength of hand increased and the strength of muscle reach over level 4. Conclusion： With the help of electro-acupuncture, acupuncture therapy can relieve pain, improve the local blood supply of nerve injury and then repair the damage of the nerve.展开更多
An efficient catalytic asymmetric dearomatizing amination of 2-naphthols and phenols catalyzed by N,N′-dioxide-copper(I)complex as a chiral catalyst was presented.A variety of optically activeβ-naphthalenone compoun...An efficient catalytic asymmetric dearomatizing amination of 2-naphthols and phenols catalyzed by N,N′-dioxide-copper(I)complex as a chiral catalyst was presented.A variety of optically activeβ-naphthalenone compounds with a nitrogen-containing quaternary carbon stereocenter were obtained with high yield and enantioselectivity under mild reaction conditions.Mechanistic studies indicated that this Csp^(2)–N dearomatizing coupling proceeds via 1,3-reductive elimination of phenolate-Cu~Ⅲ-amino intermediate in five-membered ring transition states.The origin of enantioselectivity has also been elucidated based on density functional theory calculations.展开更多
Objective This study was performed to evaluate the efficacy of robot-assisted thoracoscopic surgery(RATS)in the treatment of pulmonary sequestration(PS)in children.Methods All video-assisted thoracoscopic surgery(VATS...Objective This study was performed to evaluate the efficacy of robot-assisted thoracoscopic surgery(RATS)in the treatment of pulmonary sequestration(PS)in children.Methods All video-assisted thoracoscopic surgery(VATS)and RAST performed on patients with PS at a single center from May 2019 to July 2023 were identified.The x2 and Wilcoxon tests were used to compare the perioperative outcomes between VATS and RATS groups.Results Ninety-three patients underwent RATS while 77 patients underwent VATS.In both two groups,one patient converted to thoracotomy and no surgical mortality case.The median operation time was longer for the RATS group compared with the VATS group(75 min vs.60 min,p<0.001).A lower ratio of chest tube indwelling(61.3%vs.90.9%,p<0.001),fewer drainage days(1.0 day vs.2.0 days,p<0.001),and a shorter postoperative length of stay(5.0 days vs.6.0 days,p<0.001)were found in the RATS group than that in the VATS group.No significant difference was found in the incidence of short-term postoperative complications(hydrothorax and pneumothorax)between two groups.Conclusions RATS was safe and effective in children with PS over 6 months old and more than 7 kg.Furthermore,RATS led to better short-time postoperative outcome than VATS.Multi-institutional studies are warranted to compare differences in long-term outcomes between RATS and VATS.展开更多
Calf thymus DNA was exposed to low-energy heavy ions (N+) and 60Co-γ-rays, and the dose-effect on DNA single-strand breaks (SSB) has been investigated. The results indicate that the dose-effect curve by N+ irradiatio...Calf thymus DNA was exposed to low-energy heavy ions (N+) and 60Co-γ-rays, and the dose-effect on DNA single-strand breaks (SSB) has been investigated. The results indicate that the dose-effect curve by N+ irradiation is different from that of conventional ionizing radiation. While the curve from γ-irradiation follows exponential type, the effect curve produced by N+ ion is of 'saddle type'. The yield of DNASSB per dose unit per DNA unit remained at a certain level under different doses of γ-rays. In contrast, the DNASSB at low dosage region of N+ showed an obvious peak before it decreased rapidly to a lower level.展开更多
African swine fever virus(ASFV)infects domestic pigs and European wild boars with strong,hemorrhagic and high mortality.The primary cellular targets of ASFV is the porcine macrophages.Up to now,no commercial vaccine o...African swine fever virus(ASFV)infects domestic pigs and European wild boars with strong,hemorrhagic and high mortality.The primary cellular targets of ASFV is the porcine macrophages.Up to now,no commercial vaccine or effective treatment available to control the disease.In this study,three recombinant Saccharomyces cerevisiae(S.cerevisiae)strains expressing fused ASFV proteins-porcine Ig heavy chains were constructed and the immunogenicity of the S.cerevisiae-vectored cocktail ASFV feeding vaccine was further evaluated.To be specific,the P30-Fcγand P54-Fcαfusion proteins displaying on surface of S.cerevisiae cells were produced by fusing the Fc fragment of porcine immunoglobulin IgG1 or IgA1 with p30 or p54 gene of ASFV respectively.The recombinant P30-Fcγand P54-Fcαfusion proteins expressed by S.cerevisiae were verified by Western blotting,flow cytometry and immunofluorescence assay.Porcine immunoglobulin Fc fragment fused P30/P54 proteins elicited P30/P54-specific antibody production and induced higher mucosal immunity in swine.The absorption and phagocytosis of recombinant S.cerevisiae strains in IPEC-J2 cells or porcine alveolar macrophage(PAM)cells were significantly enhanced,too.Here,we introduce a kind of cheap and safe oral S.cerevisiae-vectored vaccine,which could activate the specific mucosal immunity for controlling ASFV infection.展开更多
Mutations or inactivation of parkin, an E3 ubiquitin ligase, are associated with familial form or sporadic Parkinson＇s disease （PD）, respectively, which manifested with the selective vulnerability of neuronal ceils...Mutations or inactivation of parkin, an E3 ubiquitin ligase, are associated with familial form or sporadic Parkinson＇s disease （PD）, respectively, which manifested with the selective vulnerability of neuronal ceils in substantia nigra （SN） and striatum （STR） regions. However, the underlying molecular mechanism linking parkin with the etiology of PD remains elusive. Here we report that p62, a critical regulator for protein quality control, inclusion body formation, selective autophagy and diverse signaling pathways, is a new substrate of parkin. P62 levels were increased in the SN and STR regions, but not in other brain regions in parkin knockout mice. Parkin directly interacts with and ubiquitinates p62 at the K13 to promote proteasomal degradation of p62 even in the absence of ATG5. Pathogenic mutations, knockdown of parkin or mutation of p62 at K13 prevented the degradation of p62. We further showed that parkin deficiency mice have pronounced loss of tyrosine hydroxylase positive neurons and have worse performance in motor test when treated with 6-hydroxydopamine hydrochloride in aged mice. These results suggest that, in addition to their critical role in regulating autophagy, p62 are subjected to parkin mediated proteasomal degradation and implicate that the dysregulation of parkin/p62 axis may involve in the selective vulnerability of neuronal cells during the onset of PD pathogenesis.展开更多
High mobility group box 1(HMGB1)has been reported to play an important role in experimental autoimmune encephalomyelitis(EAE).Astrocytes are important components of neurovascular units and tightly appose the endotheli...High mobility group box 1(HMGB1)has been reported to play an important role in experimental autoimmune encephalomyelitis(EAE).Astrocytes are important components of neurovascular units and tightly appose the endothelial cells of microvessels by their perivascular endfeet and directly regulate the functions of the blood-brain barrier.Astrocytes express more HMGB1 during EAE while the exact roles of astrocytic HMGB1 in EAE have not been well elucidated.Here,using conditional-knockout mice,we found that astrocytic HMGB1 depletion decreased morbidity,delayed the onset time,and reduced the disease score and demyelination of EAE.Meanwhile,there were fewer immune cells,especially pathogenic T cells infiltration in the central nervous system of astrocytic HMGB1 conditional-knockout EAE mice,accompanied by up-regulated expression of the tight-junction protein Claudin5 and down-regulated expression of the cell adhesion molecules ICAM1 and VCAM1 in vivo.In vitro,HMGB1 released from astrocytes decreased Claudin5 while increased ICAM1 and VCAM1 expressed by brain microvascular endothelial cells(BMECs)through TLR4 or RAGE.Taken together,our results demonstrate that HMGB1 derived from astrocytes aggravates EAE by directly influencing the immune cell infiltration-associated functions of BMECs.展开更多
文摘Objective： To analyze the cause and pathogenesis of nerve injury and find out the suitable acupuncture points. Methods： Acupuncture at the unilateral points of Hegu （LI4）, Waiguan （TB5）, Shousanli （LI10）, Quchi （LI11） and the bilateral points of Zusanli （ST36）, accompanied by method of electro-acupuncture. Results： After three courses of acupuncture treatment （30 minutes each time, 6 times each course）, the patient presented the symptoms of making a fist strongly, disappearance of wrist drop, and positive sign of lifting wrist assay in turn. Simutaneously, the grip strength of hand increased and the strength of muscle reach over level 4. Conclusion： With the help of electro-acupuncture, acupuncture therapy can relieve pain, improve the local blood supply of nerve injury and then repair the damage of the nerve.
基金supported by the National Natural Science Foundation of China(22188101)Sichuan Science and Technology Program(2021YJ0561)。
文摘An efficient catalytic asymmetric dearomatizing amination of 2-naphthols and phenols catalyzed by N,N′-dioxide-copper(I)complex as a chiral catalyst was presented.A variety of optically activeβ-naphthalenone compounds with a nitrogen-containing quaternary carbon stereocenter were obtained with high yield and enantioselectivity under mild reaction conditions.Mechanistic studies indicated that this Csp^(2)–N dearomatizing coupling proceeds via 1,3-reductive elimination of phenolate-Cu~Ⅲ-amino intermediate in five-membered ring transition states.The origin of enantioselectivity has also been elucidated based on density functional theory calculations.
基金funded by the Central Guiding Fund for Local Science and Technology Development Projects(2023ZY1058).
文摘Objective This study was performed to evaluate the efficacy of robot-assisted thoracoscopic surgery(RATS)in the treatment of pulmonary sequestration(PS)in children.Methods All video-assisted thoracoscopic surgery(VATS)and RAST performed on patients with PS at a single center from May 2019 to July 2023 were identified.The x2 and Wilcoxon tests were used to compare the perioperative outcomes between VATS and RATS groups.Results Ninety-three patients underwent RATS while 77 patients underwent VATS.In both two groups,one patient converted to thoracotomy and no surgical mortality case.The median operation time was longer for the RATS group compared with the VATS group(75 min vs.60 min,p<0.001).A lower ratio of chest tube indwelling(61.3%vs.90.9%,p<0.001),fewer drainage days(1.0 day vs.2.0 days,p<0.001),and a shorter postoperative length of stay(5.0 days vs.6.0 days,p<0.001)were found in the RATS group than that in the VATS group.No significant difference was found in the incidence of short-term postoperative complications(hydrothorax and pneumothorax)between two groups.Conclusions RATS was safe and effective in children with PS over 6 months old and more than 7 kg.Furthermore,RATS led to better short-time postoperative outcome than VATS.Multi-institutional studies are warranted to compare differences in long-term outcomes between RATS and VATS.
文摘Calf thymus DNA was exposed to low-energy heavy ions (N+) and 60Co-γ-rays, and the dose-effect on DNA single-strand breaks (SSB) has been investigated. The results indicate that the dose-effect curve by N+ irradiation is different from that of conventional ionizing radiation. While the curve from γ-irradiation follows exponential type, the effect curve produced by N+ ion is of 'saddle type'. The yield of DNASSB per dose unit per DNA unit remained at a certain level under different doses of γ-rays. In contrast, the DNASSB at low dosage region of N+ showed an obvious peak before it decreased rapidly to a lower level.
基金supported by the National Key Research and Development Program of China (2018YFD0500500)。
文摘African swine fever virus(ASFV)infects domestic pigs and European wild boars with strong,hemorrhagic and high mortality.The primary cellular targets of ASFV is the porcine macrophages.Up to now,no commercial vaccine or effective treatment available to control the disease.In this study,three recombinant Saccharomyces cerevisiae(S.cerevisiae)strains expressing fused ASFV proteins-porcine Ig heavy chains were constructed and the immunogenicity of the S.cerevisiae-vectored cocktail ASFV feeding vaccine was further evaluated.To be specific,the P30-Fcγand P54-Fcαfusion proteins displaying on surface of S.cerevisiae cells were produced by fusing the Fc fragment of porcine immunoglobulin IgG1 or IgA1 with p30 or p54 gene of ASFV respectively.The recombinant P30-Fcγand P54-Fcαfusion proteins expressed by S.cerevisiae were verified by Western blotting,flow cytometry and immunofluorescence assay.Porcine immunoglobulin Fc fragment fused P30/P54 proteins elicited P30/P54-specific antibody production and induced higher mucosal immunity in swine.The absorption and phagocytosis of recombinant S.cerevisiae strains in IPEC-J2 cells or porcine alveolar macrophage(PAM)cells were significantly enhanced,too.Here,we introduce a kind of cheap and safe oral S.cerevisiae-vectored vaccine,which could activate the specific mucosal immunity for controlling ASFV infection.
基金We are grateful to Drs. Ted Dawson and Jian Feng for generously providing the plasmids. We are also grateful to Professor Mark Bartlam from Nankai University, Tianjin, China for a critical reading of the manuscript. The research was supported by the National Basic Research Program （973 Program） （No. 2011 CB910903） from MOST and project （Grant Nos. 81130045, 31471300, 31271529, 301520103904） from the National Natural Science Foundation of China.
文摘Mutations or inactivation of parkin, an E3 ubiquitin ligase, are associated with familial form or sporadic Parkinson＇s disease （PD）, respectively, which manifested with the selective vulnerability of neuronal ceils in substantia nigra （SN） and striatum （STR） regions. However, the underlying molecular mechanism linking parkin with the etiology of PD remains elusive. Here we report that p62, a critical regulator for protein quality control, inclusion body formation, selective autophagy and diverse signaling pathways, is a new substrate of parkin. P62 levels were increased in the SN and STR regions, but not in other brain regions in parkin knockout mice. Parkin directly interacts with and ubiquitinates p62 at the K13 to promote proteasomal degradation of p62 even in the absence of ATG5. Pathogenic mutations, knockdown of parkin or mutation of p62 at K13 prevented the degradation of p62. We further showed that parkin deficiency mice have pronounced loss of tyrosine hydroxylase positive neurons and have worse performance in motor test when treated with 6-hydroxydopamine hydrochloride in aged mice. These results suggest that, in addition to their critical role in regulating autophagy, p62 are subjected to parkin mediated proteasomal degradation and implicate that the dysregulation of parkin/p62 axis may involve in the selective vulnerability of neuronal cells during the onset of PD pathogenesis.
基金the National Natural Science Foundation of China(31670876 and 82171761).
文摘High mobility group box 1(HMGB1)has been reported to play an important role in experimental autoimmune encephalomyelitis(EAE).Astrocytes are important components of neurovascular units and tightly appose the endothelial cells of microvessels by their perivascular endfeet and directly regulate the functions of the blood-brain barrier.Astrocytes express more HMGB1 during EAE while the exact roles of astrocytic HMGB1 in EAE have not been well elucidated.Here,using conditional-knockout mice,we found that astrocytic HMGB1 depletion decreased morbidity,delayed the onset time,and reduced the disease score and demyelination of EAE.Meanwhile,there were fewer immune cells,especially pathogenic T cells infiltration in the central nervous system of astrocytic HMGB1 conditional-knockout EAE mice,accompanied by up-regulated expression of the tight-junction protein Claudin5 and down-regulated expression of the cell adhesion molecules ICAM1 and VCAM1 in vivo.In vitro,HMGB1 released from astrocytes decreased Claudin5 while increased ICAM1 and VCAM1 expressed by brain microvascular endothelial cells(BMECs)through TLR4 or RAGE.Taken together,our results demonstrate that HMGB1 derived from astrocytes aggravates EAE by directly influencing the immune cell infiltration-associated functions of BMECs.
