Peanut(Arachis hypogaea L.)is a globally important oil crop.Web blotch is one of the most important foliar diseases affecting peanut,which results in serious yield losses worldwide.Breeding web blotch-resistant peanut...Peanut(Arachis hypogaea L.)is a globally important oil crop.Web blotch is one of the most important foliar diseases affecting peanut,which results in serious yield losses worldwide.Breeding web blotch-resistant peanut varieties is the most effective and economically viable method for minimizing yield losses due to web blotch.In the current study,a bulked segregant analysis with next-generation sequencing was used to analyze an F2:3 segregating population and identify candidate loci related to web blotch resistance.Based on the fine-mapping of the candidate genomic interval using kompetitive allele-specific PCR(KASP)markers,we identified a novel web blotch resistance-related locus spanning approximately 169 kb on chromosome 16.This region included four annotated genes,of which only Arahy.35VVQ3 had a non-synonymous single nucleotide polymorphism in the coding region between the two parents.Two markers(Chr.16.12872635 and Chr.16.12966357)linked to this gene were shown to be co-segregated with the resistance of peanut web blotch by 72 randomly selected recombinant inbred lines(RIL),which could be used in marker-assisted breeding of resistant peanut varieties.展开更多
Recent submillimeter dust thermal emission observations have unveiled a significant number of inter-arm massive molecular clouds in M31.However,the effectiveness of this technique is limited to its sensitivity,making ...Recent submillimeter dust thermal emission observations have unveiled a significant number of inter-arm massive molecular clouds in M31.However,the effectiveness of this technique is limited to its sensitivity,making it challenging to study more distant galaxies.This study introduces an alternative approach,utilizing optical extinctions derived from space-based telescopes,with a focus on the forthcoming China Space Station Telescope(CSST).We first demonstrate the capability of this method by constructing dust extinction maps for 17 inter-arm massive molecular clouds in M31 using the Panchromatic Hubble Andromeda Treasury data.Our analysis reveals that inter-arm massive molecular clouds with an optical extinction(A_(V)) greater than 1.6 mag exhibit a notable A_(V) excess,facilitating their identification.The majority of these inter-arm massive molecular clouds show an A_(V) around 1 mag,aligning with measurements from our JCMT data.Further validation using a mock CSST RGB star catalog confirms the method's effectiveness.We show that the derived A_(V)values using CSST z and y photometries align more closely with the input values.Molecular clouds with A_(V)> 1.6 mag can also be identified using the CSST mock data.We thus claim that future CSST observation clouds provide an effective way for the detection of inter-arm massive molecular clouds with significant optical extinction in nearby galaxies.展开更多
基金supported by the Key Research Project of the Shennong Laboratory,China(SN01-2022-03)the Henan Provincial Science and Technology R&D Program Joint Fund(Superiority Discipline Cultivation)Project,China(222301420100)+4 种基金the Major Science and Technology Projects of Henan Province,China(221100110300)the China Agriculture Research System of MOF and MARA(CARS-13)the Henan Provincial Agriculture Research System,China(S2012-5)the Outstanding Young Scientists of Henan Academy of Agricultural Sciences,China(2022YQ16)the Independent Innovation Project of the Henan Academy of Agricultural Sciences,China(2023ZC093)。
文摘Peanut(Arachis hypogaea L.)is a globally important oil crop.Web blotch is one of the most important foliar diseases affecting peanut,which results in serious yield losses worldwide.Breeding web blotch-resistant peanut varieties is the most effective and economically viable method for minimizing yield losses due to web blotch.In the current study,a bulked segregant analysis with next-generation sequencing was used to analyze an F2:3 segregating population and identify candidate loci related to web blotch resistance.Based on the fine-mapping of the candidate genomic interval using kompetitive allele-specific PCR(KASP)markers,we identified a novel web blotch resistance-related locus spanning approximately 169 kb on chromosome 16.This region included four annotated genes,of which only Arahy.35VVQ3 had a non-synonymous single nucleotide polymorphism in the coding region between the two parents.Two markers(Chr.16.12872635 and Chr.16.12966357)linked to this gene were shown to be co-segregated with the resistance of peanut web blotch by 72 randomly selected recombinant inbred lines(RIL),which could be used in marker-assisted breeding of resistant peanut varieties.
基金supported by the National Natural Science Foundation of China Nos.11988101,12373012,and 12041302supported by CMS-CSST-2021A08 and CMS-CSST-2021-B02support from NSFC with grant No.12203064。
文摘Recent submillimeter dust thermal emission observations have unveiled a significant number of inter-arm massive molecular clouds in M31.However,the effectiveness of this technique is limited to its sensitivity,making it challenging to study more distant galaxies.This study introduces an alternative approach,utilizing optical extinctions derived from space-based telescopes,with a focus on the forthcoming China Space Station Telescope(CSST).We first demonstrate the capability of this method by constructing dust extinction maps for 17 inter-arm massive molecular clouds in M31 using the Panchromatic Hubble Andromeda Treasury data.Our analysis reveals that inter-arm massive molecular clouds with an optical extinction(A_(V)) greater than 1.6 mag exhibit a notable A_(V) excess,facilitating their identification.The majority of these inter-arm massive molecular clouds show an A_(V) around 1 mag,aligning with measurements from our JCMT data.Further validation using a mock CSST RGB star catalog confirms the method's effectiveness.We show that the derived A_(V)values using CSST z and y photometries align more closely with the input values.Molecular clouds with A_(V)> 1.6 mag can also be identified using the CSST mock data.We thus claim that future CSST observation clouds provide an effective way for the detection of inter-arm massive molecular clouds with significant optical extinction in nearby galaxies.
