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刍议质谱技术发现新型蛋白质翻译后修饰研究在化学与生物学教学中的应用
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作者 王志鹏 张璇 +2 位作者 车子良 蒋振雄 马新雨 《大学化学》 CAS 2021年第6期185-193,共9页
随着质谱技术的不断发展,质谱技术已经被广泛应用于化学、材料、生命科学等众多领域,特别是其在生物化学与化学生物学领域的应用极大扩展了研究人员对于蛋白质翻译后修饰的认知。本文从基本的质谱原理入手,着重探讨了质谱技术在以组蛋... 随着质谱技术的不断发展,质谱技术已经被广泛应用于化学、材料、生命科学等众多领域,特别是其在生物化学与化学生物学领域的应用极大扩展了研究人员对于蛋白质翻译后修饰的认知。本文从基本的质谱原理入手,着重探讨了质谱技术在以组蛋白为模式蛋白的赖氨酸翻译后修饰的发现过程,并展示如何将基础有机化学和生物化学知识融入前沿科学研究。本文通过梳理不同的研究逻辑下质谱技术在蛋白翻译后修饰的研究中发挥的作用,旨在引导学生建立起将基础教学内容与实际应用相联系的意识及思维模式。 展开更多
关键词 质谱技术 串联质谱 翻译后修饰 生物化学
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Monitoring and detection of steel bridge diseases:A review
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作者 Chuang Cui Qinghua Zhang +3 位作者 Dengke Zhang Wulve Lao Lemou Wu zhenxiong jiang 《Journal of Traffic and Transportation Engineering(English Edition)》 EI CSCD 2024年第2期188-208,共21页
Fatigue,corrosion,and bolt loosening are the main causes of structural performance degradation and collapse in steel bridges.Accurate monitoring of steel bridge diseases is a basic premise for ensuring high-quality op... Fatigue,corrosion,and bolt loosening are the main causes of structural performance degradation and collapse in steel bridges.Accurate monitoring of steel bridge diseases is a basic premise for ensuring high-quality operation and maintenance of steel bridges.In this regard,a summary and analysis were conducted on the classification of steel bridge diseases,monitoring and detection methods,application statuses,and major difficulties.The main causes,research status,and development trends of steel bridge diseases are discussed.The results showed that,for fatigue crack problems,fatigue crack initiation has a small scale,high difficulty in monitoring and detection,few methods,and low accuracy.As the cracks grow,the difficulty of monitoring and detection decreases,the number of methods increases,and the accuracy improves.Fatigue crack monitoring and detection are affected by the environmental and vehicular loads.Superficial corrosion features are evident in steel bridges,and corrosion identification methods and technologies are rapidly developing.Monitoring and detecting corrosion in concealed areas is difficult and requires further improvements in monitoring and detection technologies and their accuracy.Monitoring and detection methods and supporting equipment for bolt loosening in steel bridges are rapidly developing.The development of intelligent monitoring and detection technologies and supporting equipment is an important research topic that urgently needs to be addressed for the full-lifecycle operation and maintenance of steel bridges and the sustainable development of bridge engineering.Developing new intelligent sensing components based on high-performance materials and sensing element design theory to improve the monitoring and detection perception ability is an important development direction for steel bridge monitoring and detection.Research on intelligent monitoring and detection technologies,standardized indicators,and related topics based on intelligent operations and maintenance provide great support for the development of steel-bridge disease monitoring and detection. 展开更多
关键词 Steel bridge DISEASES Fatigue crack Corrosion Bolt loosening Monitoring and detection
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Optimization of confined aquifer dewatering for long-deep excavation using simulation–optimization method
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作者 Yanxiao Sun zhenxiong jiang +4 位作者 Liyuan Tong Jiawei Sun Jia Cui Xin Zhou Songyu Liu 《Underground Space》 SCIE EI CSCD 2024年第4期246-266,共21页
The confined aquifer dewatering for long-deep excavations usually encounters challenges due to complicated geotechnical conditions,large excavation sizes,and high hydraulic pressures.To propose the most efficient sche... The confined aquifer dewatering for long-deep excavations usually encounters challenges due to complicated geotechnical conditions,large excavation sizes,and high hydraulic pressures.To propose the most efficient scheme of confined aquifer dewatering for long-deep excavations,dewatering optimizations were performed using the simulation–optimization method.An open cut tunnel of the Jiangyin-Jingjiang Yangtze River Tunnel Project was taken as an example.The methods of finite element and linear programming(LP)were combined to optimize the dewatering process.A three-dimensional finite element model was developed.After simulating the pumping tests,hydraulic conductivity was inverted.Then,necessary parameters in the LP method were determined by simulating dewatering with each pumping well,and various LP models were developed based on some important influence factors such as dewatering sequence,considered pumping wells,and pumping rate limitation.Finally,the optimal pumping rates were solved and applied to the numerical model,with induced drawdown and ground settlement computed for comparison.The results indicate that the optimization can significantly reduce the required wells in the original design.Dewatering in the deepest zone exhibits the highest efficiency for long-deep excavations with gradually varying depths.For the dewatering sequence from the shallowest to the deepest zone,more pumping wells are required but less energy is consumed.Higher quantity and more advantageous locations of pumping wells in the LP model usually result in lower total pumping rate,drawdown,and ground settlement.If more pumping wells are considered in the deepest zone,pumping rate limitation of single well will only slightly increase the total pumping rate,number of required pumping wells,drawdown,and ground settlement. 展开更多
关键词 DEWATERING Confined aquifer Simulation-optimization Linear programming Open cut tunnel
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