AIM To identify circulating micro(mi)RNAs as biological markers for prediction of severe acute pancreatitis(SAP) with acute lung injury(ALI).METHODS Twenty-four serum samples were respectively collected and classified...AIM To identify circulating micro(mi)RNAs as biological markers for prediction of severe acute pancreatitis(SAP) with acute lung injury(ALI).METHODS Twenty-four serum samples were respectively collected and classified as SAP associated with ALI and SAP without ALI, and the mi RNA expression profiles were determined by microarray analysis. These mi RNAs were validated by quantitative reverse transcriptionpolymerase chain reaction, and their putative targets were predicted by the online software Target Scan, mi Randa and Pic Tar database. Gene ontology(GO) and Kyoto encyclopedia of genes and genomes(commonly known as KEGG) were used to predict their possible functions and pathways involved.RESULTS We investigated 287 mi RNAs based on microarray data analysis. Twelve mi RNAs were differentially expressed in the patients with SAP with ALI and those with SAP without ALI. Hsa-mi R-1260 b, 762, 22-3 p, 23 b and 23 a were differently up-regulated and hsa-mi R-550 a*, 324-5 p, 484, 331-3 p, 140-3 p, 342-3 p and 150 were differently down-regulated in patients with SAP with ALI compared to those with SAP without ALI. In addition, 85 putative target genes of the significantly dysregulated mi RNAs were found by Target Scan, mi Randa and Pic Tar. Finally, GO and pathway network analysis showed that they were mainly enriched in signal transduction, metabolic processes, cytoplasm and cell membranes.CONCLUSION This is the first study to identify 12 circulating mi RNAs in patients with SAP with ALI, which may be biomarkers for prediction of ALI after SAP.展开更多
The rapid formation of a glial/fibrotic scar is one of the main factors hampering axon growth after spinal cord injury. The bidirectional Eph B2/ephrin-B2 signaling of the fibroblast-astrocyte contact-dependent intera...The rapid formation of a glial/fibrotic scar is one of the main factors hampering axon growth after spinal cord injury. The bidirectional Eph B2/ephrin-B2 signaling of the fibroblast-astrocyte contact-dependent interaction is a trigger for glial/fibrotic scar formation. In the present study, a new in vitro model was produced by coculture of fibroblasts and astrocytes wounded by scratching to mimic glial/fibrotic scar-like structures using an improved slide system. After treatment with RNAi to downregulate Eph B2, changes in glial/fibrotic scar formation and the growth of VSC4.1 motoneuron axons were examined. Following RNAi treatment, fibroblasts and astrocytes dispersed without forming a glial/fibrotic scar-like structure. Furthermore, the expression levels of neurocan, NG2 and collagen I in the coculture were reduced, and the growth of VSC4.1 motoneuron axons was enhanced. These findings suggest that suppression of Eph B2 expression by RNAi attenuates the formation of a glial/fibrotic scar and promotes axon growth. This study was approved by the Laboratory Animal Ethics Committee of Jiangsu Province, China(approval No. 2019-0506-002) on May 6, 2019.展开更多
We study two-dimensional （2D） matter-wave solitons in the mean-field models formed by electric quadrupole particles with long-range quadrupoleluadrupole interaction （QQI） in 2D free space. The existence of 2D matt...We study two-dimensional （2D） matter-wave solitons in the mean-field models formed by electric quadrupole particles with long-range quadrupoleluadrupole interaction （QQI） in 2D free space. The existence of 2D matter-wave solitons in the free space was predicted using the 2D Gross Pitaevskii Equation （GPE）. We find that the QQI solitoms have a higher mass （smaller size and higher intensity） and stronger anisotropy than the dipol^dipole interaction （DDI） solitons under the same environmental parameters. Anisotropic solitomsoliton interaction between two identical QQI solitons in 2D free space is studied. Moreover, stable anisotropic dipole solitons are observed, to our knowledge, for the first time in 2D free space under anisotropic nonlocal cubic nonlinearity.展开更多
We study the spontaneous symmetry breaking of dipolar Bose-Einstein condensates trapped in stacks of two-well systems, which may be effectively built as one-dimensional trapping lattices sliced by a repelling laser sh...We study the spontaneous symmetry breaking of dipolar Bose-Einstein condensates trapped in stacks of two-well systems, which may be effectively built as one-dimensional trapping lattices sliced by a repelling laser sheet. If the potential wells are sufficiently deep, the system is modeled by coupled discrete Gross-Pitaevskii equations with nonlocal self- and cross-interaction terms representing dipole-dipole interactions. When the dipoles are not polarized perpendicular or parallel to the lattice, the cross- interaction is asymmetric, replacing the familiar symmetric two-component solitons with a new species of cross-symmetric or -asymmetric ones. The orientation of the dipole moments and the interwell hopping rate strongly affect the shapes of the discrete two-component solitons as well as the characteristics of the cross-symmetry breaking and the associated phase transition. The sub- and super-critical types of cross-symmetry breaking can be controlled by either the hopping rate between the components or the total norm of the solitons. The effect of the interplay between the contact nonlinearity and the dipole angle on the cross-symmetry breaking is also discussed.展开更多
基金Supported by the National Natural Science Foundation of China,No.30971626 and No.81473512
文摘AIM To identify circulating micro(mi)RNAs as biological markers for prediction of severe acute pancreatitis(SAP) with acute lung injury(ALI).METHODS Twenty-four serum samples were respectively collected and classified as SAP associated with ALI and SAP without ALI, and the mi RNA expression profiles were determined by microarray analysis. These mi RNAs were validated by quantitative reverse transcriptionpolymerase chain reaction, and their putative targets were predicted by the online software Target Scan, mi Randa and Pic Tar database. Gene ontology(GO) and Kyoto encyclopedia of genes and genomes(commonly known as KEGG) were used to predict their possible functions and pathways involved.RESULTS We investigated 287 mi RNAs based on microarray data analysis. Twelve mi RNAs were differentially expressed in the patients with SAP with ALI and those with SAP without ALI. Hsa-mi R-1260 b, 762, 22-3 p, 23 b and 23 a were differently up-regulated and hsa-mi R-550 a*, 324-5 p, 484, 331-3 p, 140-3 p, 342-3 p and 150 were differently down-regulated in patients with SAP with ALI compared to those with SAP without ALI. In addition, 85 putative target genes of the significantly dysregulated mi RNAs were found by Target Scan, mi Randa and Pic Tar. Finally, GO and pathway network analysis showed that they were mainly enriched in signal transduction, metabolic processes, cytoplasm and cell membranes.CONCLUSION This is the first study to identify 12 circulating mi RNAs in patients with SAP with ALI, which may be biomarkers for prediction of ALI after SAP.
基金supported by the Priority Academic Program Development of Jiangsu Higher Education Institutes of China(PAPD)the Science and Technology Plan Project of Nantong of China,No.JC2020026(to JW)the National Science Research of Jiangsu Higher Education Institutions of China,No.19KJB310012(to RYY)。
文摘The rapid formation of a glial/fibrotic scar is one of the main factors hampering axon growth after spinal cord injury. The bidirectional Eph B2/ephrin-B2 signaling of the fibroblast-astrocyte contact-dependent interaction is a trigger for glial/fibrotic scar formation. In the present study, a new in vitro model was produced by coculture of fibroblasts and astrocytes wounded by scratching to mimic glial/fibrotic scar-like structures using an improved slide system. After treatment with RNAi to downregulate Eph B2, changes in glial/fibrotic scar formation and the growth of VSC4.1 motoneuron axons were examined. Following RNAi treatment, fibroblasts and astrocytes dispersed without forming a glial/fibrotic scar-like structure. Furthermore, the expression levels of neurocan, NG2 and collagen I in the coculture were reduced, and the growth of VSC4.1 motoneuron axons was enhanced. These findings suggest that suppression of Eph B2 expression by RNAi attenuates the formation of a glial/fibrotic scar and promotes axon growth. This study was approved by the Laboratory Animal Ethics Committee of Jiangsu Province, China(approval No. 2019-0506-002) on May 6, 2019.
文摘We study two-dimensional （2D） matter-wave solitons in the mean-field models formed by electric quadrupole particles with long-range quadrupoleluadrupole interaction （QQI） in 2D free space. The existence of 2D matter-wave solitons in the free space was predicted using the 2D Gross Pitaevskii Equation （GPE）. We find that the QQI solitoms have a higher mass （smaller size and higher intensity） and stronger anisotropy than the dipol^dipole interaction （DDI） solitons under the same environmental parameters. Anisotropic solitomsoliton interaction between two identical QQI solitons in 2D free space is studied. Moreover, stable anisotropic dipole solitons are observed, to our knowledge, for the first time in 2D free space under anisotropic nonlocal cubic nonlinearity.
基金Acknowledgements Tile authors appreciate the very useful discussion with Prof. Boris A. Malomed. This work was supported by the National Natural Science Foundation of China under Grant Nos. 11575063, 61471123, and 61575041, and the Natural Science Foundation of Guangdong Province under Grant No. 2015A030313639.
文摘We study the spontaneous symmetry breaking of dipolar Bose-Einstein condensates trapped in stacks of two-well systems, which may be effectively built as one-dimensional trapping lattices sliced by a repelling laser sheet. If the potential wells are sufficiently deep, the system is modeled by coupled discrete Gross-Pitaevskii equations with nonlocal self- and cross-interaction terms representing dipole-dipole interactions. When the dipoles are not polarized perpendicular or parallel to the lattice, the cross- interaction is asymmetric, replacing the familiar symmetric two-component solitons with a new species of cross-symmetric or -asymmetric ones. The orientation of the dipole moments and the interwell hopping rate strongly affect the shapes of the discrete two-component solitons as well as the characteristics of the cross-symmetry breaking and the associated phase transition. The sub- and super-critical types of cross-symmetry breaking can be controlled by either the hopping rate between the components or the total norm of the solitons. The effect of the interplay between the contact nonlinearity and the dipole angle on the cross-symmetry breaking is also discussed.
