Dear Editor,Submacular hemorrhage(SMH)is the accumulation of blood in the macular region caused by changes in retinal or choroidal circulation[1].SMH may be caused by age-related macular degeneration(AMD),pathological...Dear Editor,Submacular hemorrhage(SMH)is the accumulation of blood in the macular region caused by changes in retinal or choroidal circulation[1].SMH may be caused by age-related macular degeneration(AMD),pathological myopia,polypoid choroidal angiopathy(PCV)or retinal macroaneurysm[2].展开更多
AIM:To report a simple approach to actively remove high viscosity silicone oil through a 23-gauge cannula via pars plana.METHODS:Forty-eight eyes of 48 patients underwent silicone oil(5700 centistokes) removal(SOR) we...AIM:To report a simple approach to actively remove high viscosity silicone oil through a 23-gauge cannula via pars plana.METHODS:Forty-eight eyes of 48 patients underwent silicone oil(5700 centistokes) removal(SOR) were enrolled.A section of blood transfusion set was prepared to connect a standard 23-gauge cannula and vitrectomy machine.Silicone oil was removed with suction of500-mm Hg vacuum through the cannula.Main outcome measures were SOR duration,number of sutured sites,intraocular pressure(IOP),best-corrected visual acuity(BCVA),and complications.RESULTS:Silicone oil was successfully removed in all cases.The mean SOR time was 5.70±0.85 min.Nine eyes(18.75%) needed suture partial sclerotomies.No intraoperative complications were noted.Transient hypotony(≤8 mm Hg) was seen in 3 eyes(6.25%) on postoperative day 1,but all resolved within 1wk.Retinal reattachment was achieved in all cases and no other postoperative complications were noted during 3-month following-up.BCVA at the final visit improved or stabilized in all patients comparing to the preoperative level.CONCLUSION:Active removal of high viscosity silicone oil through a 23-gauge instrument cannula jointed with blood transfusion set is a practical and reliable technique when considering two sides of efficacy and safety.展开更多
AIM: To report the results of rhegmatogenous retinal detachment(RRD) repair after pars plana vitrectomy(PPV) without operative use of heavy liquid, and utilizing air tamponade in selected cases.METHODS: RRD patients w...AIM: To report the results of rhegmatogenous retinal detachment(RRD) repair after pars plana vitrectomy(PPV) without operative use of heavy liquid, and utilizing air tamponade in selected cases.METHODS: RRD patients without severity of proliferative vitreoretinopathy C2 or more underwent PPV without operative use of heavy liquid, and utilizing air tamponade were consecutively enrolled. Alternative postoperative facedown position or lateral position was required for 3-5 d.RESULTS: Totally 36 eyes of 36 patients(24 males, 66.7%) aged 53.8±10.9 y underwent this modified surgery. The mean number of retinal break was 2.1±1.3. Most of the eyes(29, 80.6%) had retinal detachment involving more than one quadrant. Twenty-two(61.1%) eyes with cataract had combined phacoemulsification and intraocular lens implantation. The mean follow up time was 4.6±1.8 mo. Two eyes with retinal redetachment underwent a second retinal repair surgery with silicone oil tamponade, yielding the primary reattachment rate to 94.4%(34/36). Six(16.7%) eyes had intraocular pressure higher than 25 mm Hg. The visual acuity(logMAR) improved from 0.98±0.74 preoperatively to 0.52±0.31 postoperatively(P<0.001). CONCLUSION: The success rate of this modified retinal repair surgery is comparable with traditional surgery. This technique can be considered for certain retinal detachment patients, since its apparent advantages included lower surgical complications, reduced surgery expenditure, shorter time for postoperative facedown position, and avoiding silicone oil removal surgery.展开更多
AIM:To investigate the safety and efficacy of sticky silicone oil(SSO)removal using a 22-gauge vein detained needle and inner limiting membrane(ILM)wrap-and-peel technique.METHODS:This retrospective consecutive case s...AIM:To investigate the safety and efficacy of sticky silicone oil(SSO)removal using a 22-gauge vein detained needle and inner limiting membrane(ILM)wrap-and-peel technique.METHODS:This retrospective consecutive case series reviewed the records of patients with a history of retinal detachment who had received silicone oil and perfluorocarbon liquid(PFCL)as intraocular tamponades.Patients were included in the analysis if they exhibited SSO remnants during silicone oil removal.The aspiration of most of the SSO remnants was performed by a 22-gauge vein detained needle.The small amounts of droplets adhered to the macula and epi-macular membrane were subsequently removed by the ILM warp-and-peel technique.The anatomical and functional outcomes,and postoperative complications were recorded.In vitro experiments were performed to simulate the formation of SSO remnants in four groups.RESULTS:Of 711 patients who underwent silicone oil removal during the study period,9 patients exhibited SSO remnants and underwent follow-up for at least 3mo.Seven eyes(78%)underwent the ILM wrap-and-peel technique to completely remove small droplets of SSO that were glued to the macula and epi-macular membrane.No obvious complications occurred.Postoperative optical coherence tomography revealed normal retinal structure in all patients.In vitro analyses showed that balanced salt solution and prolonged vibration(for 1wk)had the strongest effects on silicone oil and PFCL compound opacities.CONCLUSION:SSO remnants could be removed in an intact manner and without complications,using a vein detained needle-assisted and ILM wrap-and-peel technique.The findings suggest that PFCL and infusion fluid should be completely removed before silicone oil injection to prevent SSO formation.展开更多
AIM:To determine the effects of epidermal growth factor(EGF)on the proliferation and migration of Müller cell line Moorfields/Institute of Ophthalmology-Müller 1(MIO-M1),and its related molecular mechanisms ...AIM:To determine the effects of epidermal growth factor(EGF)on the proliferation and migration of Müller cell line Moorfields/Institute of Ophthalmology-Müller 1(MIO-M1),and its related molecular mechanisms under normal and oxidative stress conditions.METHODS:Müller cells were cultured with different concentrations of EGF in the presence or absence of varied amounts of H2O2and glucose oxidase(GO)which induced oxidative stress.The proliferation and migration of Müller cells were examined by 5-Bromo-2-deoxy Uridine(BrdU),MTT assay,Transwell assay and scratch wound healing assays.The cell viability was determined with the MTT assay.The secretion of EGF by Müller cells was evaluated by ELISA.Western blot was performed to detect the activation of extracellular regulated protein kinases(ERK)1/2 and Akt signal pathways.RESULTS:EGF stimulated the proliferation and migration of Müller cells in a concentration-dependent manner in vitro.Under oxidative damage condition,2h of pretreatment with 10-100 ng/mL EGF can mostly inhibit 50% lethal dose of 0.08 mmol/L H2O2-induced cell damage.The Western blot results showed that after Müller cells were exposed to varying EGF for 24h,Akt and ERK1/2 were phosphorylated in a dose-dependent manner.In the presence of the LY294002,the potent PI3K inhibitor,the p-Akt was significantly attenuated.CONCLUSION:EGF may induce the proliferation and migration of human Müller cells through the Akt and the ERK1/2 signal pathways,and induce PI3K-mediated glioprotective effect under oxidative stress.展开更多
文摘Dear Editor,Submacular hemorrhage(SMH)is the accumulation of blood in the macular region caused by changes in retinal or choroidal circulation[1].SMH may be caused by age-related macular degeneration(AMD),pathological myopia,polypoid choroidal angiopathy(PCV)or retinal macroaneurysm[2].
基金Supported by the National Nature Science Foundation(No.81473295)Science Technology project of Zhejiang Province(No.2014C33260)Wenzhou Science and Technology Bureau(No.Y20140142)
文摘AIM:To report a simple approach to actively remove high viscosity silicone oil through a 23-gauge cannula via pars plana.METHODS:Forty-eight eyes of 48 patients underwent silicone oil(5700 centistokes) removal(SOR) were enrolled.A section of blood transfusion set was prepared to connect a standard 23-gauge cannula and vitrectomy machine.Silicone oil was removed with suction of500-mm Hg vacuum through the cannula.Main outcome measures were SOR duration,number of sutured sites,intraocular pressure(IOP),best-corrected visual acuity(BCVA),and complications.RESULTS:Silicone oil was successfully removed in all cases.The mean SOR time was 5.70±0.85 min.Nine eyes(18.75%) needed suture partial sclerotomies.No intraoperative complications were noted.Transient hypotony(≤8 mm Hg) was seen in 3 eyes(6.25%) on postoperative day 1,but all resolved within 1wk.Retinal reattachment was achieved in all cases and no other postoperative complications were noted during 3-month following-up.BCVA at the final visit improved or stabilized in all patients comparing to the preoperative level.CONCLUSION:Active removal of high viscosity silicone oil through a 23-gauge instrument cannula jointed with blood transfusion set is a practical and reliable technique when considering two sides of efficacy and safety.
基金Supported by the Innovation Research Project of the Eye Hospital of Wenzhou Medical University (No. YNCX201308)
文摘AIM: To report the results of rhegmatogenous retinal detachment(RRD) repair after pars plana vitrectomy(PPV) without operative use of heavy liquid, and utilizing air tamponade in selected cases.METHODS: RRD patients without severity of proliferative vitreoretinopathy C2 or more underwent PPV without operative use of heavy liquid, and utilizing air tamponade were consecutively enrolled. Alternative postoperative facedown position or lateral position was required for 3-5 d.RESULTS: Totally 36 eyes of 36 patients(24 males, 66.7%) aged 53.8±10.9 y underwent this modified surgery. The mean number of retinal break was 2.1±1.3. Most of the eyes(29, 80.6%) had retinal detachment involving more than one quadrant. Twenty-two(61.1%) eyes with cataract had combined phacoemulsification and intraocular lens implantation. The mean follow up time was 4.6±1.8 mo. Two eyes with retinal redetachment underwent a second retinal repair surgery with silicone oil tamponade, yielding the primary reattachment rate to 94.4%(34/36). Six(16.7%) eyes had intraocular pressure higher than 25 mm Hg. The visual acuity(logMAR) improved from 0.98±0.74 preoperatively to 0.52±0.31 postoperatively(P<0.001). CONCLUSION: The success rate of this modified retinal repair surgery is comparable with traditional surgery. This technique can be considered for certain retinal detachment patients, since its apparent advantages included lower surgical complications, reduced surgery expenditure, shorter time for postoperative facedown position, and avoiding silicone oil removal surgery.
基金the Wenzhou Basic ScientificResearch Program (No.20211003).
文摘AIM:To investigate the safety and efficacy of sticky silicone oil(SSO)removal using a 22-gauge vein detained needle and inner limiting membrane(ILM)wrap-and-peel technique.METHODS:This retrospective consecutive case series reviewed the records of patients with a history of retinal detachment who had received silicone oil and perfluorocarbon liquid(PFCL)as intraocular tamponades.Patients were included in the analysis if they exhibited SSO remnants during silicone oil removal.The aspiration of most of the SSO remnants was performed by a 22-gauge vein detained needle.The small amounts of droplets adhered to the macula and epi-macular membrane were subsequently removed by the ILM warp-and-peel technique.The anatomical and functional outcomes,and postoperative complications were recorded.In vitro experiments were performed to simulate the formation of SSO remnants in four groups.RESULTS:Of 711 patients who underwent silicone oil removal during the study period,9 patients exhibited SSO remnants and underwent follow-up for at least 3mo.Seven eyes(78%)underwent the ILM wrap-and-peel technique to completely remove small droplets of SSO that were glued to the macula and epi-macular membrane.No obvious complications occurred.Postoperative optical coherence tomography revealed normal retinal structure in all patients.In vitro analyses showed that balanced salt solution and prolonged vibration(for 1wk)had the strongest effects on silicone oil and PFCL compound opacities.CONCLUSION:SSO remnants could be removed in an intact manner and without complications,using a vein detained needle-assisted and ILM wrap-and-peel technique.The findings suggest that PFCL and infusion fluid should be completely removed before silicone oil injection to prevent SSO formation.
基金Supported by Natural Science Foundation of Zhejiang Province,China(LY13H120004)
文摘AIM:To determine the effects of epidermal growth factor(EGF)on the proliferation and migration of Müller cell line Moorfields/Institute of Ophthalmology-Müller 1(MIO-M1),and its related molecular mechanisms under normal and oxidative stress conditions.METHODS:Müller cells were cultured with different concentrations of EGF in the presence or absence of varied amounts of H2O2and glucose oxidase(GO)which induced oxidative stress.The proliferation and migration of Müller cells were examined by 5-Bromo-2-deoxy Uridine(BrdU),MTT assay,Transwell assay and scratch wound healing assays.The cell viability was determined with the MTT assay.The secretion of EGF by Müller cells was evaluated by ELISA.Western blot was performed to detect the activation of extracellular regulated protein kinases(ERK)1/2 and Akt signal pathways.RESULTS:EGF stimulated the proliferation and migration of Müller cells in a concentration-dependent manner in vitro.Under oxidative damage condition,2h of pretreatment with 10-100 ng/mL EGF can mostly inhibit 50% lethal dose of 0.08 mmol/L H2O2-induced cell damage.The Western blot results showed that after Müller cells were exposed to varying EGF for 24h,Akt and ERK1/2 were phosphorylated in a dose-dependent manner.In the presence of the LY294002,the potent PI3K inhibitor,the p-Akt was significantly attenuated.CONCLUSION:EGF may induce the proliferation and migration of human Müller cells through the Akt and the ERK1/2 signal pathways,and induce PI3K-mediated glioprotective effect under oxidative stress.
