AIM:To test the pathogenicity of pseudorabies virus(PRV)variant HN1201 and compare its pathogenicity with a classical PRV Fa strain.METHODS:The pathogenicity of the newly-emerging PRV variant HN1201 was evaluated by d...AIM:To test the pathogenicity of pseudorabies virus(PRV)variant HN1201 and compare its pathogenicity with a classical PRV Fa strain.METHODS:The pathogenicity of the newly-emerging PRV variant HN1201 was evaluated by different inoculating routes,virus loads,and ages of pigs.The classical PRV Fa strain was then used to compare with HN1201 to determine pathogenicity.Clinical symptoms after virus infection were recorded daily and average daily body weight was used to measure the growth performance of pigs.At necropsy,gross pathology and histopathology were used to evaluate the severity of tissue damage caused by virus infection.RESULTS:The results showed that the efficient infection method of RPV HN1201 was via intranasal inoculation at 107 TCID50,and that the virus has high pathogenicity to 35-to 127-d old pigs.Compared with Fa strain,pigs infected with HN1201 showed more severe clinical symptoms and pathological lesions.Immunochemistry results revealed HN1201 had more abundant antigen distribution in extensive organs.CONCLUSION:All of the above results suggest that PRV variant HN1201 was more pathogenic to pigs than the classical Fa strain.展开更多
Background:Accumulating evidence has revealed that circulating microRNAs(miRNAs)can serve as non-invasive biomarkers for cancer diagnosis.This study aimed to identify differentially expressed miRNAs in serum which mig...Background:Accumulating evidence has revealed that circulating microRNAs(miRNAs)can serve as non-invasive biomarkers for cancer diagnosis.This study aimed to identify differentially expressed miRNAs in serum which might become potential biomarkers for non-invasive diagnosis of papillary thyroid carcinoma(PTC).Methods:The experiment was carried out between 2015 and 2017.In the screening stage,the Exiqon miRNA quantitative real-time polymerase chain reaction(qPCR)panel was applied to select candidate miRNAs.In the following training,testing,and external validation stages,the serum samples of 100 patients and 96 healthy controls(HCs)were analyzed to compare the expression levels of the identified miRNAs.The areas under the receiver operating characteristic curves(AUCs)were calculated to assess the diagnostic value of the identified signature.Results:Three miRNAs(miR-25-3p,miR-296-5p,and miR-92a-3p)in serum were consistently up-regulated in PTC patients compared with HCs.A three-miRNA panel was constructed by logistic regression analysis and showed better diagnostic performance than a single miRNA for PTC detection.The AUCs of the panel were 0.727,0.771,and 0.862 for the training,testing,and external validation stage,respectively.Meanwhile,the panel showed stable capability in differentiating PTC patients from patients with benign goiters,with an AUC as high as 0.969.For further exploration,the three identified miRNAs were analyzed in tissue samples(23 PTC vs.23 HCs)and serum-derived exosomes samples(24 PTC vs.24 HCs),and the altered expression in the tumor also indicated their close relationship with PTC disease.Conclusion:We identify a three-miRNA panel in serum which might serve as a promising biomarker for PTC diagnosis.展开更多
基金Supported by Major Science and Technology Program in Henan Province,No.131100110200
文摘AIM:To test the pathogenicity of pseudorabies virus(PRV)variant HN1201 and compare its pathogenicity with a classical PRV Fa strain.METHODS:The pathogenicity of the newly-emerging PRV variant HN1201 was evaluated by different inoculating routes,virus loads,and ages of pigs.The classical PRV Fa strain was then used to compare with HN1201 to determine pathogenicity.Clinical symptoms after virus infection were recorded daily and average daily body weight was used to measure the growth performance of pigs.At necropsy,gross pathology and histopathology were used to evaluate the severity of tissue damage caused by virus infection.RESULTS:The results showed that the efficient infection method of RPV HN1201 was via intranasal inoculation at 107 TCID50,and that the virus has high pathogenicity to 35-to 127-d old pigs.Compared with Fa strain,pigs infected with HN1201 showed more severe clinical symptoms and pathological lesions.Immunochemistry results revealed HN1201 had more abundant antigen distribution in extensive organs.CONCLUSION:All of the above results suggest that PRV variant HN1201 was more pathogenic to pigs than the classical Fa strain.
基金grants from the National Natural Science Foundation of China (No. 81672400, and No. 81702364)the Natural Science Foundation of Jiangsu Provincial Department of Education (No. BK20171085)。
文摘Background:Accumulating evidence has revealed that circulating microRNAs(miRNAs)can serve as non-invasive biomarkers for cancer diagnosis.This study aimed to identify differentially expressed miRNAs in serum which might become potential biomarkers for non-invasive diagnosis of papillary thyroid carcinoma(PTC).Methods:The experiment was carried out between 2015 and 2017.In the screening stage,the Exiqon miRNA quantitative real-time polymerase chain reaction(qPCR)panel was applied to select candidate miRNAs.In the following training,testing,and external validation stages,the serum samples of 100 patients and 96 healthy controls(HCs)were analyzed to compare the expression levels of the identified miRNAs.The areas under the receiver operating characteristic curves(AUCs)were calculated to assess the diagnostic value of the identified signature.Results:Three miRNAs(miR-25-3p,miR-296-5p,and miR-92a-3p)in serum were consistently up-regulated in PTC patients compared with HCs.A three-miRNA panel was constructed by logistic regression analysis and showed better diagnostic performance than a single miRNA for PTC detection.The AUCs of the panel were 0.727,0.771,and 0.862 for the training,testing,and external validation stage,respectively.Meanwhile,the panel showed stable capability in differentiating PTC patients from patients with benign goiters,with an AUC as high as 0.969.For further exploration,the three identified miRNAs were analyzed in tissue samples(23 PTC vs.23 HCs)and serum-derived exosomes samples(24 PTC vs.24 HCs),and the altered expression in the tumor also indicated their close relationship with PTC disease.Conclusion:We identify a three-miRNA panel in serum which might serve as a promising biomarker for PTC diagnosis.
