BACKGROUND Prostate cancer(PCa)is a widespread malignancy,predominantly affecting elderly males,and current methods for diagnosis and treatment of this disease continue to fall short.The marker Ki-67(MKI67)has been pr...BACKGROUND Prostate cancer(PCa)is a widespread malignancy,predominantly affecting elderly males,and current methods for diagnosis and treatment of this disease continue to fall short.The marker Ki-67(MKI67)has been previously demonstrated to correlate with the proliferation and metastasis of various cancer cells,including those of PCa.Hence,verifying the association between MKI67 and the diagnosis and prognosis of PCa,using bioinformatics databases and clinical data analysis,carries significant clinical implications.AIM To explore the diagnostic and prognostic efficacy of antigens identified by MKI67 expression in PCa.METHODS For cohort 1,the efficacy of MKI67 diagnosis was evaluated using data from The Cancer Genome Atlas(TCGA)and Genotype-Tissue Expression(GTEx)databases.For cohort 2,the diagnostic and prognostic power of MKI67 expression was further validated using data from 271 patients with clinical PCa.RESULTS In cohort 1,MKI67 expression was correlated with prostate-specific antigen(PSA),Gleason Score,T stage,and N stage.The receiver operating characteristic(ROC)curve showed a strong diagnostic ability,and the Kaplan-Meier method demonstrated that MKI67 expression was negatively associated with the progression-free interval(PFI).The time-ROC curve displayed a weak prognostic capability for MKI67 expression in PCa.In cohort 2,MKI67 expression was significantly related to the Gleason Score,T stage,and N stage;however,it was negatively associated with the PFI.The time-ROC curve revealed the stronger prognostic capability of MKI67 in patients with PCa.Multivariate COX regression analysis was performed to select risk factors,including PSA level,N stage,and MKI67 expression.A nomogram was established to predict the 3-year PFI.CONCLUSION MKI67 expression was positively associated with the Gleason Score,T stage,and N stage and showed a strong diagnostic and prognostic ability in PCa.展开更多
Accurate flux density calibration is essential for precise analysis and interpretation of observations across different observation modes and instruments.In this research,we first introduce the flux calibration model ...Accurate flux density calibration is essential for precise analysis and interpretation of observations across different observation modes and instruments.In this research,we first introduce the flux calibration model that incorporated in Hi FAST pipeline,and designed for processing HⅠ21 cm spectra.Furthermore,we investigate different calibration techniques and assess the dependence of the gain parameter on the time and environmental factors.A comparison is carried out in various observation modes(e.g.,tracking and scanning modes)to determine the flux density gain(G),revealing insignificant discrepancies in G among different methods.Long-term monitoring data shows a linear correlation between G and atmospheric temperature.After subtracting the G-Temperature dependence,the dispersion of G is reduced to<3%over a one-year timescale.The stability of the receiver response of Five-hundred-meter Aperture Spherical radio Telescope(FAST)is considered sufficient to facilitate HⅠobservations that can accommodate a moderate error in flux calibration(e.g.,>~5%)when utilizing a constant G for calibration purposes.Our study will serve as a useful addition to the results provided by Jiang et al.Detailed measurement of G for the 19 beams of FAST,covering the frequency range 1000-1500 MHz,can be found on the Hi FAST homepage:https://hifast.readthedocs.io/fluxgain.展开更多
This is an erratum to the published paper titled“Marker Ki-67 is a potential biomarker for the diagnosis and prognosis of prostate cancer based on two cohorts”.We have discovered that Reference 4 was cited incorrect...This is an erratum to the published paper titled“Marker Ki-67 is a potential biomarker for the diagnosis and prognosis of prostate cancer based on two cohorts”.We have discovered that Reference 4 was cited incorrectly.We sincerely apologize for this inadvertent error.Please note that these corrections do not impact our results.展开更多
AIM: To investigate the mutually inductive interactions that occur between the lens and retinal tissue during the development of the vertebrate eye. METHODS: Cre-positive mice were mated with Cre-negative mice to gene...AIM: To investigate the mutually inductive interactions that occur between the lens and retinal tissue during the development of the vertebrate eye. METHODS: Cre-positive mice were mated with Cre-negative mice to generate 50% Cre-positive (conditional knockout, CKO) and 50% Cre-negative offspring (wild type, WT). The embryos were fixed in 4% paraformaldehyde, embedded in paraffin, and sectioned to a thickness of 4 mu m. The sections were processed for hematoxylin and eosin staining. The primary antibody used for immunofluorescence detection was sc-9305 bone morphogenetic proteins (bmp7) (Santa Cruz, US). The secondary antibody was IF-0314 aG0IgG/FITC (Santa Cruz) in combination with the primary antibody. Bright-field and fluorescent images were taken. RESULTS: Changes in the lens and retina were associated with specific alterations to the expression of type IA BMP receptor [BMPR-IA (ALK3)], which have already been implicated in eye growth. BMPR-IA was required for lens and retinal growth, but was not essential for the formation of lens. We observed that the expression of Bmp7 in the embryonic retina was reduced in the ALK3 lens of CKO mice. This phenomenon became increasingly visible in accordance with embryo development. This apparent alteration was present at stage E15.5. CONCLUSION: ALK3 is essential for lens and retinal growth. Mutually inductive interactions between the lens and retina are present in the developing mouse eye.展开更多
BACKGROUND Pneumonia of uncertain cause has been reported in Wuhan,China since the beginning of early December 2019.In early January 2020,a novel strain ofβ-coronavirus was identified by the Chinese Center for Diseas...BACKGROUND Pneumonia of uncertain cause has been reported in Wuhan,China since the beginning of early December 2019.In early January 2020,a novel strain ofβ-coronavirus was identified by the Chinese Center for Disease Control and Prevention from the pharyngeal swab specimens of patients,which was recently named severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).There is evidence of human-to-human transmission and familial cluster outbreak of SARSCoV-2 infection.The World Health Organization(WHO)recently declared the SARS-CoV-2 epidemic a global health emergency.As of February 17,2020,71329 laboratory-confirmed cases(in 25 countries,including the United States and Germany)have been reported globally.Other than its rapid transmission,the epidemiological and clinical characteristics of coronavirus disease 2019(COVID-19)remain unclear.In December 2019,coronavirus disease(named COVID-19 by the WHO)associated with the SARS-CoV-2 emerged in Wuhan,China and spread quickly across the country.AIM To analyze the epidemiological and clinical characteristics of confirmed cases of this disease in Liaoning province,a Chinese region about 1800 km north of Wuhan.METHODS The clinical data of 56 laboratory-confirmed COVID-19 cases due to 2019-nCoV infection were analyzed.The cases originated from eight cities in Liaoning province.RESULTS The median age of the patients was 45 years,and 57.1%of them were male.No patient had been in direct contact with wild animals.Among them,23 patients(41.1%)had resided in or traveled to Wuhan,27 cases(48.2%)had been in contact with confirmed COVID-19 patients,5 cases(8.9%)had been in contact with confirmed patients with a contact history to COVID-19 patients,and 1 case(1.8%)had no apparent history of exposure.Fever(75.0%)and cough(60.7%)were the most common symptoms.The typical manifestations in lung computed tomography(CT)included ground-glass opacity and patchy shadows,with 67.8%of them being bilateral.Among the patients in the cohort,78.6%showed reduction in their lymphocyte counts,57.1%showed increases in their C-reactive protein levels,and 50.0%showed decreases in their blood albumin levels.Eleven patients(19.6%)were admitted to intensive care unit,2 patients(3.5%)progressed to acute respiratory distress syndrome,4 patients(7.1%)were equipped with non-invasive mechanical ventilation,and 1 patient(1.8)received extracorporeal membrane oxygenation support.There were 5 mild cases(5/56,8.9%),40 moderate cases(40/56,71.4%),10 severe cases(10/56,17.9%),and 1 critical case(1/56,1.8%).No deaths were reported.CONCLUSION SARS-CoV-2 can be transmitted among humans.Most COVID-19 patients show symptoms of fever,cough,lymphocyte reduction,and typical lung CT manifestations.Most are moderate cases.The seriousness of the disease(as indicated by blood oxygen saturation,respiratory rate,oxygenation index,blood lymphocyte count,and lesions shown in lung CT)is related to history of living in or traveling to Wuhan,underlying diseases,admittance to intensive care unit,and mechanical ventilation.展开更多
We present high resolution （- 1″） H2CO maser and 5 GHz radio continuum observations toward nearby merging galaxy NGC 6240 made with the Very Large Array in an A configuration. Two concentrations of H2CO emission at...We present high resolution （- 1″） H2CO maser and 5 GHz radio continuum observations toward nearby merging galaxy NGC 6240 made with the Very Large Array in an A configuration. Two concentrations of H2CO emission at about a 6or level have been detected, one of which is associated with the strongest CO peak in the overlap region while the other is about 2″ southwest of the southem galaxy. Both H2CO concentrations are associated with near infrared H2 emission, which is thought to be from shocked molecular gas. The total H2CO line luminosity in NGC 6240 is about 60% of that in Arp 220. Based on the distribution of H2CO emission in NGC 6240, which has both active galactic nuclei and an extreme starburst, the H2CO megamaser is likely to be related to the effect of the starburst instead of nuclear activity. Radio continuum cannot be the inversion mechanism of H2CO megamasers, because the two H2CO concentrations in NGC 6240 are not associated with radio continuum emission. Instead, with the association of near infrared H2 emission, shock dynamics may produce the inverted population of H2CO needed to generate megamasers.展开更多
We present simultaneous observations of 12CO,13CO and C18O J=1-0 emission in 11 nearby（cz1000 km s-1） bright infrared galaxies.Both 12CO and 13CO are detected in the centers of all the galaxies,except for 13CO in NG...We present simultaneous observations of 12CO,13CO and C18O J=1-0 emission in 11 nearby（cz1000 km s-1） bright infrared galaxies.Both 12CO and 13CO are detected in the centers of all the galaxies,except for 13CO in NGC 3031. We have also detected C18O,CSJ=2-1 and HCO＋J=1-0 emission in the nuclear regions of M82 and M51.These are the first systematical extragalactic detections of 12CO and its isotopes from the PMO 14 m telescope.We have conducted half-beam-spaced mapping of M82 over an area of 4×2.5 and major axis mapping of NGC 3627,NGC 3628,NGC 4631 and M51.The radial distributions of 12CO and 13CO in NGC 3627,NGC 3628 and M51 can be well fitted by an exponential profile. The 12CO/13CO intensity ratio,R,decreases monotonically with the galactocentric radius in all mapped sources.The average R in the center and disk of the galaxies are 9.9±3.0 and 5.6±1.9,respectively,much lower than the peculiar R（～24） found in the center of M82.The intensity ratios of 13CO/C18O,13CO/HCO＋and 13CO/CS（either our or literature data） show little variation with galactocentric radius,in sharp contrast with the greatly varied R.This supports the notion that the observed gradient in R could be the result of the variations of the physical conditions across the disks.The H2 column density derived from C18O shows that the Galactic standard conversion factor（X-factor） overestimates the amount of the molecular gas in M82 by a factor of～2.5.These observations suggest that the X-factor in active star-forming regions（i.e.,nuclear regions） should be lower than that in normal star-forming disks and the gradient in R can be used to trace the variations of the X-factor.展开更多
Background： Najanalgesin, a toxin isolated from the venom ofNaja nqja atra, has been shown to exert significant analgesic effects in a neuropathic pain model in rats. However, the molecular mechanism underlying this ...Background： Najanalgesin, a toxin isolated from the venom ofNaja nqja atra, has been shown to exert significant analgesic effects in a neuropathic pain model in rats. However, the molecular mechanism underlying this protective effect ofnajanalgesin is poorly understood. The present study sought to evaluate the intracellular signaling pathways that are involved in the antinociceptive effect of najanalgesin on neuropathic pain. Methods： The antinociceptive properties of najanalgesin were tested in hind paw withdrawal thresholds in response to mechanical stimulation. We analyzed the participation of the mitogen-activated protein kinase p38, extracellular-regulated kinase （ERK）, and c-Jun N-terminal kinase （JNK） by western blot analysis. This inhibition of JNK was confirmed by immunohistochemistry. Results： The phosphorylation levels of INK （as well as its downstream molecule c-Jun）, p38, and ERK were significantly increased after injury. Najanalgesin only inhibited JNK and c-Jun phosphorylation but had no effect on either ERK or p38. This inhibition of JNK was confirmed by immunohistochemistry, which suggested that the antinociceptive effect of najanalgesin on spinal nerve ligation-induced neuropathic pain in rats is associated with JNK activation in the spinal cord. Conclusion： The antinociceptive effect of najanalgesin thnctions by inhibiting the JNK in a neuropathic pain model.展开更多
基金Supported by Suzhou Science and Technology Project,No.SYS2019053.
文摘BACKGROUND Prostate cancer(PCa)is a widespread malignancy,predominantly affecting elderly males,and current methods for diagnosis and treatment of this disease continue to fall short.The marker Ki-67(MKI67)has been previously demonstrated to correlate with the proliferation and metastasis of various cancer cells,including those of PCa.Hence,verifying the association between MKI67 and the diagnosis and prognosis of PCa,using bioinformatics databases and clinical data analysis,carries significant clinical implications.AIM To explore the diagnostic and prognostic efficacy of antigens identified by MKI67 expression in PCa.METHODS For cohort 1,the efficacy of MKI67 diagnosis was evaluated using data from The Cancer Genome Atlas(TCGA)and Genotype-Tissue Expression(GTEx)databases.For cohort 2,the diagnostic and prognostic power of MKI67 expression was further validated using data from 271 patients with clinical PCa.RESULTS In cohort 1,MKI67 expression was correlated with prostate-specific antigen(PSA),Gleason Score,T stage,and N stage.The receiver operating characteristic(ROC)curve showed a strong diagnostic ability,and the Kaplan-Meier method demonstrated that MKI67 expression was negatively associated with the progression-free interval(PFI).The time-ROC curve displayed a weak prognostic capability for MKI67 expression in PCa.In cohort 2,MKI67 expression was significantly related to the Gleason Score,T stage,and N stage;however,it was negatively associated with the PFI.The time-ROC curve revealed the stronger prognostic capability of MKI67 in patients with PCa.Multivariate COX regression analysis was performed to select risk factors,including PSA level,N stage,and MKI67 expression.A nomogram was established to predict the 3-year PFI.CONCLUSION MKI67 expression was positively associated with the Gleason Score,T stage,and N stage and showed a strong diagnostic and prognostic ability in PCa.
基金the support of the China National Key Program for Science and Technology Research and Development of China(2022YFA1602901,2023YFA1608204)the National Natural Science Foundation of China(Nos.11988101,11873051,12125302,12373011,12041305,12173016)the CAS Project for Young Scientists in Basic Research grant(No.YSBR-062)。
文摘Accurate flux density calibration is essential for precise analysis and interpretation of observations across different observation modes and instruments.In this research,we first introduce the flux calibration model that incorporated in Hi FAST pipeline,and designed for processing HⅠ21 cm spectra.Furthermore,we investigate different calibration techniques and assess the dependence of the gain parameter on the time and environmental factors.A comparison is carried out in various observation modes(e.g.,tracking and scanning modes)to determine the flux density gain(G),revealing insignificant discrepancies in G among different methods.Long-term monitoring data shows a linear correlation between G and atmospheric temperature.After subtracting the G-Temperature dependence,the dispersion of G is reduced to<3%over a one-year timescale.The stability of the receiver response of Five-hundred-meter Aperture Spherical radio Telescope(FAST)is considered sufficient to facilitate HⅠobservations that can accommodate a moderate error in flux calibration(e.g.,>~5%)when utilizing a constant G for calibration purposes.Our study will serve as a useful addition to the results provided by Jiang et al.Detailed measurement of G for the 19 beams of FAST,covering the frequency range 1000-1500 MHz,can be found on the Hi FAST homepage:https://hifast.readthedocs.io/fluxgain.
文摘This is an erratum to the published paper titled“Marker Ki-67 is a potential biomarker for the diagnosis and prognosis of prostate cancer based on two cohorts”.We have discovered that Reference 4 was cited incorrectly.We sincerely apologize for this inadvertent error.Please note that these corrections do not impact our results.
基金Supported by National Natural Science Foundation of China(No.30872836)Natural Science Foundation of Liaoning Province,China(No.201102054)
文摘AIM: To investigate the mutually inductive interactions that occur between the lens and retinal tissue during the development of the vertebrate eye. METHODS: Cre-positive mice were mated with Cre-negative mice to generate 50% Cre-positive (conditional knockout, CKO) and 50% Cre-negative offspring (wild type, WT). The embryos were fixed in 4% paraformaldehyde, embedded in paraffin, and sectioned to a thickness of 4 mu m. The sections were processed for hematoxylin and eosin staining. The primary antibody used for immunofluorescence detection was sc-9305 bone morphogenetic proteins (bmp7) (Santa Cruz, US). The secondary antibody was IF-0314 aG0IgG/FITC (Santa Cruz) in combination with the primary antibody. Bright-field and fluorescent images were taken. RESULTS: Changes in the lens and retina were associated with specific alterations to the expression of type IA BMP receptor [BMPR-IA (ALK3)], which have already been implicated in eye growth. BMPR-IA was required for lens and retinal growth, but was not essential for the formation of lens. We observed that the expression of Bmp7 in the embryonic retina was reduced in the ALK3 lens of CKO mice. This phenomenon became increasingly visible in accordance with embryo development. This apparent alteration was present at stage E15.5. CONCLUSION: ALK3 is essential for lens and retinal growth. Mutually inductive interactions between the lens and retina are present in the developing mouse eye.
文摘BACKGROUND Pneumonia of uncertain cause has been reported in Wuhan,China since the beginning of early December 2019.In early January 2020,a novel strain ofβ-coronavirus was identified by the Chinese Center for Disease Control and Prevention from the pharyngeal swab specimens of patients,which was recently named severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).There is evidence of human-to-human transmission and familial cluster outbreak of SARSCoV-2 infection.The World Health Organization(WHO)recently declared the SARS-CoV-2 epidemic a global health emergency.As of February 17,2020,71329 laboratory-confirmed cases(in 25 countries,including the United States and Germany)have been reported globally.Other than its rapid transmission,the epidemiological and clinical characteristics of coronavirus disease 2019(COVID-19)remain unclear.In December 2019,coronavirus disease(named COVID-19 by the WHO)associated with the SARS-CoV-2 emerged in Wuhan,China and spread quickly across the country.AIM To analyze the epidemiological and clinical characteristics of confirmed cases of this disease in Liaoning province,a Chinese region about 1800 km north of Wuhan.METHODS The clinical data of 56 laboratory-confirmed COVID-19 cases due to 2019-nCoV infection were analyzed.The cases originated from eight cities in Liaoning province.RESULTS The median age of the patients was 45 years,and 57.1%of them were male.No patient had been in direct contact with wild animals.Among them,23 patients(41.1%)had resided in or traveled to Wuhan,27 cases(48.2%)had been in contact with confirmed COVID-19 patients,5 cases(8.9%)had been in contact with confirmed patients with a contact history to COVID-19 patients,and 1 case(1.8%)had no apparent history of exposure.Fever(75.0%)and cough(60.7%)were the most common symptoms.The typical manifestations in lung computed tomography(CT)included ground-glass opacity and patchy shadows,with 67.8%of them being bilateral.Among the patients in the cohort,78.6%showed reduction in their lymphocyte counts,57.1%showed increases in their C-reactive protein levels,and 50.0%showed decreases in their blood albumin levels.Eleven patients(19.6%)were admitted to intensive care unit,2 patients(3.5%)progressed to acute respiratory distress syndrome,4 patients(7.1%)were equipped with non-invasive mechanical ventilation,and 1 patient(1.8)received extracorporeal membrane oxygenation support.There were 5 mild cases(5/56,8.9%),40 moderate cases(40/56,71.4%),10 severe cases(10/56,17.9%),and 1 critical case(1/56,1.8%).No deaths were reported.CONCLUSION SARS-CoV-2 can be transmitted among humans.Most COVID-19 patients show symptoms of fever,cough,lymphocyte reduction,and typical lung CT manifestations.Most are moderate cases.The seriousness of the disease(as indicated by blood oxygen saturation,respiratory rate,oxygenation index,blood lymphocyte count,and lesions shown in lung CT)is related to history of living in or traveling to Wuhan,underlying diseases,admittance to intensive care unit,and mechanical ventilation.
基金supported by the National Basic Research Program of China (973 Program, 2012CB821800)the Natural Science Foundation of China(Grant Nos. 10833006 and 11173013)
文摘We present high resolution （- 1″） H2CO maser and 5 GHz radio continuum observations toward nearby merging galaxy NGC 6240 made with the Very Large Array in an A configuration. Two concentrations of H2CO emission at about a 6or level have been detected, one of which is associated with the strongest CO peak in the overlap region while the other is about 2″ southwest of the southem galaxy. Both H2CO concentrations are associated with near infrared H2 emission, which is thought to be from shocked molecular gas. The total H2CO line luminosity in NGC 6240 is about 60% of that in Arp 220. Based on the distribution of H2CO emission in NGC 6240, which has both active galactic nuclei and an extreme starburst, the H2CO megamaser is likely to be related to the effect of the starburst instead of nuclear activity. Radio continuum cannot be the inversion mechanism of H2CO megamasers, because the two H2CO concentrations in NGC 6240 are not associated with radio continuum emission. Instead, with the association of near infrared H2 emission, shock dynamics may produce the inverted population of H2CO needed to generate megamasers.
基金Supported by the National Natural Science Foundation of Chinafunded by the National Science Foundation of China(Distinguished Young Scholars,Nos.10425313,10833006 and 10621303)the Hundred Talent Program of the Chinese Academy of Sciences
文摘We present simultaneous observations of 12CO,13CO and C18O J=1-0 emission in 11 nearby（cz1000 km s-1） bright infrared galaxies.Both 12CO and 13CO are detected in the centers of all the galaxies,except for 13CO in NGC 3031. We have also detected C18O,CSJ=2-1 and HCO＋J=1-0 emission in the nuclear regions of M82 and M51.These are the first systematical extragalactic detections of 12CO and its isotopes from the PMO 14 m telescope.We have conducted half-beam-spaced mapping of M82 over an area of 4×2.5 and major axis mapping of NGC 3627,NGC 3628,NGC 4631 and M51.The radial distributions of 12CO and 13CO in NGC 3627,NGC 3628 and M51 can be well fitted by an exponential profile. The 12CO/13CO intensity ratio,R,decreases monotonically with the galactocentric radius in all mapped sources.The average R in the center and disk of the galaxies are 9.9±3.0 and 5.6±1.9,respectively,much lower than the peculiar R（～24） found in the center of M82.The intensity ratios of 13CO/C18O,13CO/HCO＋and 13CO/CS（either our or literature data） show little variation with galactocentric radius,in sharp contrast with the greatly varied R.This supports the notion that the observed gradient in R could be the result of the variations of the physical conditions across the disks.The H2 column density derived from C18O shows that the Galactic standard conversion factor（X-factor） overestimates the amount of the molecular gas in M82 by a factor of～2.5.These observations suggest that the X-factor in active star-forming regions（i.e.,nuclear regions） should be lower than that in normal star-forming disks and the gradient in R can be used to trace the variations of the X-factor.
基金This study was supported by the grants from the National Natural Science Foundation of China，the Shandong Province National Natural Science Foundation，the project from Weifang Science and Technology Bureau，the Weifang Medical Technology Innovation Project
文摘Background： Najanalgesin, a toxin isolated from the venom ofNaja nqja atra, has been shown to exert significant analgesic effects in a neuropathic pain model in rats. However, the molecular mechanism underlying this protective effect ofnajanalgesin is poorly understood. The present study sought to evaluate the intracellular signaling pathways that are involved in the antinociceptive effect of najanalgesin on neuropathic pain. Methods： The antinociceptive properties of najanalgesin were tested in hind paw withdrawal thresholds in response to mechanical stimulation. We analyzed the participation of the mitogen-activated protein kinase p38, extracellular-regulated kinase （ERK）, and c-Jun N-terminal kinase （JNK） by western blot analysis. This inhibition of JNK was confirmed by immunohistochemistry. Results： The phosphorylation levels of INK （as well as its downstream molecule c-Jun）, p38, and ERK were significantly increased after injury. Najanalgesin only inhibited JNK and c-Jun phosphorylation but had no effect on either ERK or p38. This inhibition of JNK was confirmed by immunohistochemistry, which suggested that the antinociceptive effect of najanalgesin on spinal nerve ligation-induced neuropathic pain in rats is associated with JNK activation in the spinal cord. Conclusion： The antinociceptive effect of najanalgesin thnctions by inhibiting the JNK in a neuropathic pain model.
