Objective:The integration of training in theory and practice across the medical education spectrum is being encouraged to increase student understanding and skills in the sciences.This study aimed to determine the dec...Objective:The integration of training in theory and practice across the medical education spectrum is being encouraged to increase student understanding and skills in the sciences.This study aimed to determine the deciding factors that drive students'perceived advantages in class to improve precision education and the teaching model.Methods:A mixed strategy of an existing flipped classroom(FC)and a case-based learning(CBL)model was conducted in a medical morphology curriculum for 575 postgraduate students.The subjective learning evaluation of the individuals(learning time,engagement,study interest and concentration,and professional integration)was collected and analyzed after FC-CBL model learning.Results:The results from the general evaluation showed promising results of the medical morphology in the FC-CBL model.Students felt more engaged by instructors in person and benefited in terms of time-saving,flexible arrangements,and professional improvement.Our study contributed to the FC-CBL model in Research Design in postgraduate training in 4 categories:1)advancing a guideline of precision teaching according to individual characteristics;2)revealing whether a learning background is needed for a Research Design course to guide setting up a preliminary course;3)understanding the perceived advantages and their interfaces;and 4)barriers and/or improvement to implement the FC-CBL model in the Research Design class,such as a richer description of e-learning and hands-on practice.Conclusion:Undertaking a FC-CBL combined model could be a useful addition to pedagogy for medical morphology learning in postgraduate training.展开更多
In this paper,we investigate the equilibrium stability of a Filippov-type system having multiple stick regions based upon a smooth and discontinuous(SD) oscillator with dry friction.The sets of equilibrium states of t...In this paper,we investigate the equilibrium stability of a Filippov-type system having multiple stick regions based upon a smooth and discontinuous(SD) oscillator with dry friction.The sets of equilibrium states of the system are analyzed together with Coulomb friction conditions in both( f_n,f_s) and(x,˙x) planes.In the stability analysis,Lyapunov functions are constructed to derive the instability for the equilibrium set of the hyperbolic type and La Salle's invariance principle is employed to obtain the stability of the nonhyperbolic type.Analysis demonstrates the existence of a thick stable manifold and the interior stability of the hyperbolic equilibrium set due to the attractive sliding mode of the Filippov property,and also shows that the unstable manifolds of the hyperbolic-type are that of the endpoints with their saddle property.Numerical calculations show a good agreement with the theoretical analysis and an excellent efficien y of the approach for equilibrium states in this particular Filippov system.Furthermore,the equilibrium bifurcations are presented to demonstrate the transition between the smooth and the discontinuous regimes.展开更多
Stem cell-based therapy raises hopes for a better approach to promoting tissue repair and functional recovery.However,transplanted stem cells show a high death percentage,creating challenges to successful transplantat...Stem cell-based therapy raises hopes for a better approach to promoting tissue repair and functional recovery.However,transplanted stem cells show a high death percentage,creating challenges to successful transplantation and prognosis.Thus,it is necessary to investigate the mechanisms underlying stem cell death,such as apoptotic cascade activation,excessive autophagy,inflammatory response,reactive oxygen species,excitotoxicity,and ischemia/hypoxia.Targeting the molecular pathways involved may be an efficient strategy to enhance stem cell viability and maximize transplantation success.Notably,a more complex network of cell death receives more attention than one crucial pathway in determining stem cell fate,highlighting the challenges in exploring mechanisms and therapeutic targets.In this review,we focus on programmed cell death in transplanted stem cells.We also discuss some promising strategies and challenges in promoting survival for further study.展开更多
AIM: To assess the effect of notoginsenoside R1 on hepatic microcirculatory disturbance induced by gut ischemia/reperfusion (I/R) in mice.METHODS: The superior mesenteric artery (SMA) of C57/BL mice was ligated for 15...AIM: To assess the effect of notoginsenoside R1 on hepatic microcirculatory disturbance induced by gut ischemia/reperfusion (I/R) in mice.METHODS: The superior mesenteric artery (SMA) of C57/BL mice was ligated for 15 min to induce gut ischemia followed by 30-min reperfusion. In another set of experiments, R1 was continuously infused (10 mg/kg per hour) from 10 min before I/R until the end of the investigation to study the influence of R1 on hepatic microcirculatory disturbance induced by gut I/R. Hepatic microcirculation was observed by inverted microscopy, and the vascular diameter, red blood cell (RBC) velocity and sinusoid perfusion were estimated. Leukocyte rolling and adhesion were observed under a laser confocal microscope. Thirty and 60 min after reperfusion, lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate transaminase (AST) in peripheral blood were determined. The expression of adhesion molecules CD11b/CD18 in neutrophils and tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1) in plasma were evaluated by flow cytometry. E-selectin and intercellular adhesion molecule-1 (ICAM-1) in hepatic tissue were examined by immunofluorescence. RESULTS: After gut I/R, the diameters of terminal portal venules and central veins, RBC velocity and the number of perfused sinusoids were decreased, while the leukocyte rolling and adhesion, the expression of E-selectin in hepatic vessels and CD18 in neutrophils, IL-6, MCP-1, LDH, ALT and AST were increased. R1 treatment attenuated these alterations except for IL-6 and MCP-1.CONCLUSION: R1 prevents I/R-induced hepatic microcirculation disturbance and hepatocyte injury. The effect of R1 is related to its inhibition of leukocyte rolling and adhesion by inhibiting the expression of E-selectin in endothelium and CD18 in neutrophils.展开更多
In this paper, the periodic solutions of the smooth and discontinuous (SD) oscillator, which is a strongly irrational nonlinear system are discussed for the system having a viscous damping and an external harmonic exc...In this paper, the periodic solutions of the smooth and discontinuous (SD) oscillator, which is a strongly irrational nonlinear system are discussed for the system having a viscous damping and an external harmonic excitation. A four dimensional averaging method is employed by using the complete Jacobian elliptic integrals directly to obtain the perturbed primary responses which bifurcate from both the hyperbolic saddle and the non-hyperbolic centres of the unperturbed system. The stability of these periodic solutions is analysed by examining the four dimensional averaged equation using Lyapunov method. The results presented herein this paper are valid for both smooth ( α > 0) and discontinuous ( α = 0) stages providing the answer to the question why the averaging theorem spectacularly fails for the case of medium strength of external forcing in the Duffing system analysed by Holmes. Numerical calculations show a good agreement with the theoretical predictions and an excellent efficiency of the analysis for this particular system, which also suggests the analysis is applicable to strongly nonlinear systems.展开更多
Hyaluronidase(HAase)has been regarded as one of the potential cancer biomarkers.In this study,a novel enzyme-responded controlled release electrochemical biosensor has been constructed for HAase detection.Methylene bl...Hyaluronidase(HAase)has been regarded as one of the potential cancer biomarkers.In this study,a novel enzyme-responded controlled release electrochemical biosensor has been constructed for HAase detection.Methylene blue(MB)can be adsorbed on hyaluronic acid(HA)to form macromolecular HA–MB complex first through electrostatic interaction between anionic HA and positively charged MB.The prepared complex cannot be filtered out through centrifugal ultrafiltration,and small electrochemical signal can be recorded from the ultrafiltrate.With the help of HAase,HA–MB complex can be cleaved into small pieces through enzyme digestion.The small pieces of HA–MB complex can be easily released from the macromolecular HA–MB complex to the ultrafiltrate by centrifugal ultrafiltration.Then,the enhanced electrochemical signal from MB can be detected in the above-mentioned ultrafiltrate.The enhanced diff erential pulse voltammetric response of the system has a linear relationship with HAase concentration in the range of 4.0–60 U·mL^(-1),with the limit of detection of 0.28 U·mL^(-1).The developed strategy has been successfully applied to detect HAase activity in urine samples.展开更多
基金supported by grants from the Hunan Province Academic Degree and Graduate Education Reform Project(No.2020JGYB028)the National Natural Science Foundation of China(No.81971891,No.82172196,No.81772134)+1 种基金the Key Laboratory of Emergency and Trauma(Hainan Medical University)of the Ministry of Education(No.KLET-202108)the College Students'Innovation and Entrepreneurship Project(No.S20210026020013).
文摘Objective:The integration of training in theory and practice across the medical education spectrum is being encouraged to increase student understanding and skills in the sciences.This study aimed to determine the deciding factors that drive students'perceived advantages in class to improve precision education and the teaching model.Methods:A mixed strategy of an existing flipped classroom(FC)and a case-based learning(CBL)model was conducted in a medical morphology curriculum for 575 postgraduate students.The subjective learning evaluation of the individuals(learning time,engagement,study interest and concentration,and professional integration)was collected and analyzed after FC-CBL model learning.Results:The results from the general evaluation showed promising results of the medical morphology in the FC-CBL model.Students felt more engaged by instructors in person and benefited in terms of time-saving,flexible arrangements,and professional improvement.Our study contributed to the FC-CBL model in Research Design in postgraduate training in 4 categories:1)advancing a guideline of precision teaching according to individual characteristics;2)revealing whether a learning background is needed for a Research Design course to guide setting up a preliminary course;3)understanding the perceived advantages and their interfaces;and 4)barriers and/or improvement to implement the FC-CBL model in the Research Design class,such as a richer description of e-learning and hands-on practice.Conclusion:Undertaking a FC-CBL combined model could be a useful addition to pedagogy for medical morphology learning in postgraduate training.
基金supported by the National Natural Science Foundation of China(Grant 11372082)the National Basic Research Program of China(Grant 2015CB057405)
文摘In this paper,we investigate the equilibrium stability of a Filippov-type system having multiple stick regions based upon a smooth and discontinuous(SD) oscillator with dry friction.The sets of equilibrium states of the system are analyzed together with Coulomb friction conditions in both( f_n,f_s) and(x,˙x) planes.In the stability analysis,Lyapunov functions are constructed to derive the instability for the equilibrium set of the hyperbolic type and La Salle's invariance principle is employed to obtain the stability of the nonhyperbolic type.Analysis demonstrates the existence of a thick stable manifold and the interior stability of the hyperbolic equilibrium set due to the attractive sliding mode of the Filippov property,and also shows that the unstable manifolds of the hyperbolic-type are that of the endpoints with their saddle property.Numerical calculations show a good agreement with the theoretical analysis and an excellent efficien y of the approach for equilibrium states in this particular Filippov system.Furthermore,the equilibrium bifurcations are presented to demonstrate the transition between the smooth and the discontinuous regimes.
基金Supported by the National Natural Science Foundation of China,No.81772134,No.81971891,and No.81571939.
文摘Stem cell-based therapy raises hopes for a better approach to promoting tissue repair and functional recovery.However,transplanted stem cells show a high death percentage,creating challenges to successful transplantation and prognosis.Thus,it is necessary to investigate the mechanisms underlying stem cell death,such as apoptotic cascade activation,excessive autophagy,inflammatory response,reactive oxygen species,excitotoxicity,and ischemia/hypoxia.Targeting the molecular pathways involved may be an efficient strategy to enhance stem cell viability and maximize transplantation success.Notably,a more complex network of cell death receives more attention than one crucial pathway in determining stem cell fate,highlighting the challenges in exploring mechanisms and therapeutic targets.In this review,we focus on programmed cell death in transplanted stem cells.We also discuss some promising strategies and challenges in promoting survival for further study.
基金Supported by Tianjin Tasly Group, Tianjin, China
文摘AIM: To assess the effect of notoginsenoside R1 on hepatic microcirculatory disturbance induced by gut ischemia/reperfusion (I/R) in mice.METHODS: The superior mesenteric artery (SMA) of C57/BL mice was ligated for 15 min to induce gut ischemia followed by 30-min reperfusion. In another set of experiments, R1 was continuously infused (10 mg/kg per hour) from 10 min before I/R until the end of the investigation to study the influence of R1 on hepatic microcirculatory disturbance induced by gut I/R. Hepatic microcirculation was observed by inverted microscopy, and the vascular diameter, red blood cell (RBC) velocity and sinusoid perfusion were estimated. Leukocyte rolling and adhesion were observed under a laser confocal microscope. Thirty and 60 min after reperfusion, lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate transaminase (AST) in peripheral blood were determined. The expression of adhesion molecules CD11b/CD18 in neutrophils and tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1) in plasma were evaluated by flow cytometry. E-selectin and intercellular adhesion molecule-1 (ICAM-1) in hepatic tissue were examined by immunofluorescence. RESULTS: After gut I/R, the diameters of terminal portal venules and central veins, RBC velocity and the number of perfused sinusoids were decreased, while the leukocyte rolling and adhesion, the expression of E-selectin in hepatic vessels and CD18 in neutrophils, IL-6, MCP-1, LDH, ALT and AST were increased. R1 treatment attenuated these alterations except for IL-6 and MCP-1.CONCLUSION: R1 prevents I/R-induced hepatic microcirculation disturbance and hepatocyte injury. The effect of R1 is related to its inhibition of leukocyte rolling and adhesion by inhibiting the expression of E-selectin in endothelium and CD18 in neutrophils.
基金supported by the National Natural Science Foundation of China(11072065)
文摘In this paper, the periodic solutions of the smooth and discontinuous (SD) oscillator, which is a strongly irrational nonlinear system are discussed for the system having a viscous damping and an external harmonic excitation. A four dimensional averaging method is employed by using the complete Jacobian elliptic integrals directly to obtain the perturbed primary responses which bifurcate from both the hyperbolic saddle and the non-hyperbolic centres of the unperturbed system. The stability of these periodic solutions is analysed by examining the four dimensional averaged equation using Lyapunov method. The results presented herein this paper are valid for both smooth ( α > 0) and discontinuous ( α = 0) stages providing the answer to the question why the averaging theorem spectacularly fails for the case of medium strength of external forcing in the Duffing system analysed by Holmes. Numerical calculations show a good agreement with the theoretical predictions and an excellent efficiency of the analysis for this particular system, which also suggests the analysis is applicable to strongly nonlinear systems.
基金financially supported by National Sciences Foundation of China(21575025,21575027)the cooperative project of production and study in University of Fujian Province(2018Y4007)STS Key Project of Fujian Province(2017T3007)
文摘Hyaluronidase(HAase)has been regarded as one of the potential cancer biomarkers.In this study,a novel enzyme-responded controlled release electrochemical biosensor has been constructed for HAase detection.Methylene blue(MB)can be adsorbed on hyaluronic acid(HA)to form macromolecular HA–MB complex first through electrostatic interaction between anionic HA and positively charged MB.The prepared complex cannot be filtered out through centrifugal ultrafiltration,and small electrochemical signal can be recorded from the ultrafiltrate.With the help of HAase,HA–MB complex can be cleaved into small pieces through enzyme digestion.The small pieces of HA–MB complex can be easily released from the macromolecular HA–MB complex to the ultrafiltrate by centrifugal ultrafiltration.Then,the enhanced electrochemical signal from MB can be detected in the above-mentioned ultrafiltrate.The enhanced diff erential pulse voltammetric response of the system has a linear relationship with HAase concentration in the range of 4.0–60 U·mL^(-1),with the limit of detection of 0.28 U·mL^(-1).The developed strategy has been successfully applied to detect HAase activity in urine samples.
