Implantable hydrogel-based bioelectronics(IHB)can precisely monitor human health and diagnose diseases.However,achieving biodegradability,biocompatibility,and high conformality with soft tissues poses significant chal...Implantable hydrogel-based bioelectronics(IHB)can precisely monitor human health and diagnose diseases.However,achieving biodegradability,biocompatibility,and high conformality with soft tissues poses significant challenges for IHB.Gelatin is the most suitable candidate for IHB since it is a collagen hydrolysate and a substantial part of the extracellular matrix found naturally in most tissues.This study used 3D printing ultrafine fiber networks with metamaterial design to embed into ultra-low elastic modulus hydrogel to create a novel gelatin-based conductive film(GCF)with mechanical programmability.The regulation of GCF nearly covers soft tissue mechanics,an elastic modulus from 20 to 420 kPa,and a Poisson’s ratio from-0.25 to 0.52.The negative Poisson’s ratio promotes conformality with soft tissues to improve the efficiency of biological interfaces.The GCF can monitor heartbeat signals and respiratory rate by determining cardiac deformation due to its high conformability.Notably,the gelatin characteristics of the biodegradable GCF enable the sensor to monitor and support tissue restoration.The GCF metamaterial design offers a unique idea for bioelectronics to develop implantable sensors that integrate monitoring and tissue repair and a customized method for endowing implanted sensors to be highly conformal with soft tissues.展开更多
Large bone defect regeneration has always been recognized as a challenging clinical problem due to the difficulty of revascularization.Conventional treatments exhibit certain inherent disadvantages(e.g.,secondary inju...Large bone defect regeneration has always been recognized as a challenging clinical problem due to the difficulty of revascularization.Conventional treatments exhibit certain inherent disadvantages(e.g.,secondary injury,immunization,and potential infections).However,three-dimensional(3D)printing technology as an emerging field can serve as an effective approach to achieve satisfactory revascularization while making up for the above limitations.A wide variety of methods can be used to facilitate blood supply during the design of a 3D-printed scaffold.Importantly,the scaffold structure lays a foundation for the entire printing object;any method to promote angiogenesis can be effective only if it is based on well-designed scaffolds.In this review,different designs related to angiogenesis are summarized by collecting the literature from recent years.The 3D-printed scaffolds are classified into four major categories and discussed in detail,from elementary porous scaffolds to the most advanced bone-like scaffolds.Finally,structural design suggestions to achieve rapid angiogenesis are proposed by analyzing the above architectures.This review can provide a reference for organizations or individual academics to achieve improved bone defect repair and regeneration using 3D printing.展开更多
The crosstalk between the nerve and stomatognathic systems plays a more important role in organismal health than previously appreciated with the presence of emerging concept of the“brain-oral axis”.A deeper understa...The crosstalk between the nerve and stomatognathic systems plays a more important role in organismal health than previously appreciated with the presence of emerging concept of the“brain-oral axis”.A deeper understanding of the intricate interaction between the nervous system and the stomatognathic system is warranted,considering their significant developmental homology and anatomical proximity,and the more complex innervation of the jawbone compared to other skeletons.In this review,we provide an in-depth look at studies concerning neurodevelopment,craniofacial development,and congenital anomalies that occur when the two systems develop abnormally.It summarizes the cross-regulation between nerves and jawbones and the effects of various states of the jawbone on intrabony nerve distribution.Diseases closely related to both the nervous system and the stomatognathic system are divided into craniofacial diseases caused by neurological illnesses,and neurological diseases caused by an aberrant stomatognathic system.The two-way relationships between common diseases,such as periodontitis and neurodegenerative disorders,and depression and oral diseases were also discussed.This review provides valuable insights into novel strategies for neuro-skeletal tissue engineering and early prevention and treatment of orofacial and neurological diseases.展开更多
Chinese milk vetch( Astragalus sinicus L.) is a commonly used green manure crop in paddy fields in southern China and it has the effect of fostering fertility and soil. This paper firstly summarized advances in resear...Chinese milk vetch( Astragalus sinicus L.) is a commonly used green manure crop in paddy fields in southern China and it has the effect of fostering fertility and soil. This paper firstly summarized advances in research of new high quality and yield varieties of germplasm resources of Chinese milk vetch. Then,on the basis of current situation,it came up with recommendations for collection,storage and promotion,as well as application in the current ecological agriculture,including establishing germplasm resource bank and improving germplasm resource evaluation system.展开更多
Multiple signaling pathways are involved in the regulation of cell proliferation and differentiation in odontogenesis and dental tissue renewal,but the details of these mechanisms remain unknown.Here,we investigated t...Multiple signaling pathways are involved in the regulation of cell proliferation and differentiation in odontogenesis and dental tissue renewal,but the details of these mechanisms remain unknown.Here,we investigated the expression patterns of a transcription factor,Krüppel-like factor 6(KLF6),during the development of murine tooth germ and its function in odontoblastic differentiation.KLF6 was almost ubiquitously expressed in odontoblasts at various stages,and it was co-expressed with P21(to varying degrees)in mouse dental germ.To determine the function of Klf6,overexpression and knockdown experiments were performed in a mouse dental papilla cell line(iMDP-3).Klf6 functioned as a promoter of odontoblastic differentiation and inhibited the proliferation and cell cycle progression of i MDP-3 through p21 upregulation.Dual-luciferase reporter assay and chromatin immunoprecipitation showed that Klf6 directly activates p21 transcription.Additionally,the in vivo study showed that KLF6 and P21were also co-expressed in odontoblasts around the reparative dentin.In conclusion,Klf6 regulates the transcriptional activity of p21,thus promoting the cell proliferation to odontoblastic differentiation transition in vitro.This study provides a theoretical basis for odontoblast differentiation and the formation of reparative dentine regeneration.展开更多
Stem cell based transplants effectively regenerate tissues;however, limitations such as immune rejection and teratoma formation prevent their application. Extracellular vesicles (EVs)-mediated acellular tissue regener...Stem cell based transplants effectively regenerate tissues;however, limitations such as immune rejection and teratoma formation prevent their application. Extracellular vesicles (EVs)-mediated acellular tissue regeneration is a promising alternative to stem cell based transplants. Although neural EGFL-like 1 (Nell1) is known to contribute to the osteogenic differentiation of bone marrow stem cells (BMSCs), it remains unknown whether EVs are involved in this process. Here, we present that EVs derived from Nell1-modified BMSCs (Nell1/EVs) have a stronger ability to promote BMSC osteogenesis owing to miR-25–5p downregulation. MiR-25–5p inhibits osteogenesis by targeting Smad2 and suppressing the SMAD and extracellular signal-related kinase 1 and 2 (ERK1/2) pathway activation. In addition, we demonstrate that the 3D-Nell1/EV-hydrogel system is beneficial for bone regeneration in vivo, probably stemming from a slow, continuous release and high concentration of EVs in the bone defect area. Thus, our results have shown the potential of Nell1/EVs as a novel acellular bone regeneration strategy. Mechanistically, the identification of miR-25-5p-SMAD2 signaling axis expands the knowledge of Nell1/EVs induced osteogenesis.展开更多
Contact engineering is of critical importance for two-dimensional(2D)transition metal dichalcogenide(TMD)-based devices.However,there are only a few solutions to overcome this obstacle because of the complexity of the...Contact engineering is of critical importance for two-dimensional(2D)transition metal dichalcogenide(TMD)-based devices.However,there are only a few solutions to overcome this obstacle because of the complexity of the TMD-contact interface.In this work,we propose a novel method using a soft plasma treatment followed by the seamless deposition of a metal electrode to reduce the contact resistance of MoS_(2)field effect transistors(FETs).The treated FETs exhibit three times higher mobility than the control FETs without plasma treatment.The soft plasma treatment can remove the facial sulfur atoms and expose the middle Mo atoms so that they come into direct contact with the metal electrode,thus greatly improving the contact behavior.First-principles calculation is also performed to support the experimental results.Our potentially scalable strategy can be extended to the whole family of TMD based FETs to provide a possible route of device processsing technology for 2D device application.展开更多
基金This work was sponsored by the National Natural Science Foundation of China(No.52235007,52325504)the Science Fund for Creative Research Groups of the National Natural Science Foundation of China(No.T2121004).
文摘Implantable hydrogel-based bioelectronics(IHB)can precisely monitor human health and diagnose diseases.However,achieving biodegradability,biocompatibility,and high conformality with soft tissues poses significant challenges for IHB.Gelatin is the most suitable candidate for IHB since it is a collagen hydrolysate and a substantial part of the extracellular matrix found naturally in most tissues.This study used 3D printing ultrafine fiber networks with metamaterial design to embed into ultra-low elastic modulus hydrogel to create a novel gelatin-based conductive film(GCF)with mechanical programmability.The regulation of GCF nearly covers soft tissue mechanics,an elastic modulus from 20 to 420 kPa,and a Poisson’s ratio from-0.25 to 0.52.The negative Poisson’s ratio promotes conformality with soft tissues to improve the efficiency of biological interfaces.The GCF can monitor heartbeat signals and respiratory rate by determining cardiac deformation due to its high conformability.Notably,the gelatin characteristics of the biodegradable GCF enable the sensor to monitor and support tissue restoration.The GCF metamaterial design offers a unique idea for bioelectronics to develop implantable sensors that integrate monitoring and tissue repair and a customized method for endowing implanted sensors to be highly conformal with soft tissues.
基金supported by the Zhejiang Province Key Research and Development Program(No.2021C03059)。
文摘Large bone defect regeneration has always been recognized as a challenging clinical problem due to the difficulty of revascularization.Conventional treatments exhibit certain inherent disadvantages(e.g.,secondary injury,immunization,and potential infections).However,three-dimensional(3D)printing technology as an emerging field can serve as an effective approach to achieve satisfactory revascularization while making up for the above limitations.A wide variety of methods can be used to facilitate blood supply during the design of a 3D-printed scaffold.Importantly,the scaffold structure lays a foundation for the entire printing object;any method to promote angiogenesis can be effective only if it is based on well-designed scaffolds.In this review,different designs related to angiogenesis are summarized by collecting the literature from recent years.The 3D-printed scaffolds are classified into four major categories and discussed in detail,from elementary porous scaffolds to the most advanced bone-like scaffolds.Finally,structural design suggestions to achieve rapid angiogenesis are proposed by analyzing the above architectures.This review can provide a reference for organizations or individual academics to achieve improved bone defect repair and regeneration using 3D printing.
基金supported by the Key Research and Development Program in Zhejiang Province(No.2021C03059)the Funds of the Central Government Guiding Local Science and Technology Development(No.2023ZY1060)National Natural Science Foundation of China(No.81801011).
文摘The crosstalk between the nerve and stomatognathic systems plays a more important role in organismal health than previously appreciated with the presence of emerging concept of the“brain-oral axis”.A deeper understanding of the intricate interaction between the nervous system and the stomatognathic system is warranted,considering their significant developmental homology and anatomical proximity,and the more complex innervation of the jawbone compared to other skeletons.In this review,we provide an in-depth look at studies concerning neurodevelopment,craniofacial development,and congenital anomalies that occur when the two systems develop abnormally.It summarizes the cross-regulation between nerves and jawbones and the effects of various states of the jawbone on intrabony nerve distribution.Diseases closely related to both the nervous system and the stomatognathic system are divided into craniofacial diseases caused by neurological illnesses,and neurological diseases caused by an aberrant stomatognathic system.The two-way relationships between common diseases,such as periodontitis and neurodegenerative disorders,and depression and oral diseases were also discussed.This review provides valuable insights into novel strategies for neuro-skeletal tissue engineering and early prevention and treatment of orofacial and neurological diseases.
基金Supported by State Key Research and Development Program of Ministry of Science and Technology(2017YFD0200808)National Undergraduate Training Program for Innovation and Entrepreneurship(201510410011)
文摘Chinese milk vetch( Astragalus sinicus L.) is a commonly used green manure crop in paddy fields in southern China and it has the effect of fostering fertility and soil. This paper firstly summarized advances in research of new high quality and yield varieties of germplasm resources of Chinese milk vetch. Then,on the basis of current situation,it came up with recommendations for collection,storage and promotion,as well as application in the current ecological agriculture,including establishing germplasm resource bank and improving germplasm resource evaluation system.
基金supported by the National Key R&D Program of China(No.2018YFC1105103)the Natural Science Foundation of Zhejiang Province,China(No.LY19H140004)the National Natural Science Foundation of China(Grant No.81771118)。
文摘Multiple signaling pathways are involved in the regulation of cell proliferation and differentiation in odontogenesis and dental tissue renewal,but the details of these mechanisms remain unknown.Here,we investigated the expression patterns of a transcription factor,Krüppel-like factor 6(KLF6),during the development of murine tooth germ and its function in odontoblastic differentiation.KLF6 was almost ubiquitously expressed in odontoblasts at various stages,and it was co-expressed with P21(to varying degrees)in mouse dental germ.To determine the function of Klf6,overexpression and knockdown experiments were performed in a mouse dental papilla cell line(iMDP-3).Klf6 functioned as a promoter of odontoblastic differentiation and inhibited the proliferation and cell cycle progression of i MDP-3 through p21 upregulation.Dual-luciferase reporter assay and chromatin immunoprecipitation showed that Klf6 directly activates p21 transcription.Additionally,the in vivo study showed that KLF6 and P21were also co-expressed in odontoblasts around the reparative dentin.In conclusion,Klf6 regulates the transcriptional activity of p21,thus promoting the cell proliferation to odontoblastic differentiation transition in vitro.This study provides a theoretical basis for odontoblast differentiation and the formation of reparative dentine regeneration.
基金supported by the National Key R&D Program of China(2019YFA0308402 and 2018YFA0305604)the Innovation Program for Quantum Science and Technology(2021ZD0302403)+1 种基金the National Natural Science Foundation of China(11934001,92265106,11774010,and 11921005)Beijing Municipal Natural Science Foundation(JQ20002)。
基金This work was jointly supported by the National Natural Science Foundation of China,China(grant nos.81771118).
文摘Stem cell based transplants effectively regenerate tissues;however, limitations such as immune rejection and teratoma formation prevent their application. Extracellular vesicles (EVs)-mediated acellular tissue regeneration is a promising alternative to stem cell based transplants. Although neural EGFL-like 1 (Nell1) is known to contribute to the osteogenic differentiation of bone marrow stem cells (BMSCs), it remains unknown whether EVs are involved in this process. Here, we present that EVs derived from Nell1-modified BMSCs (Nell1/EVs) have a stronger ability to promote BMSC osteogenesis owing to miR-25–5p downregulation. MiR-25–5p inhibits osteogenesis by targeting Smad2 and suppressing the SMAD and extracellular signal-related kinase 1 and 2 (ERK1/2) pathway activation. In addition, we demonstrate that the 3D-Nell1/EV-hydrogel system is beneficial for bone regeneration in vivo, probably stemming from a slow, continuous release and high concentration of EVs in the bone defect area. Thus, our results have shown the potential of Nell1/EVs as a novel acellular bone regeneration strategy. Mechanistically, the identification of miR-25-5p-SMAD2 signaling axis expands the knowledge of Nell1/EVs induced osteogenesis.
基金the National Key Research and Development Program(No.2016YFA0203900)the Shanghai Municipal Science and Technology Commission(No.18JC1410300)+5 种基金the National Natural Science Foundation of China(Nos.61874154,61874060,61911530220,U1932159)financial support from the Fundamental Research Funds for the Central Universities of China(No.JUSRP51726B)the“111 Project”(No.B12018)the Postgraduate Research and Practice Innovation Program of Jiangsu Province(No.KYCX181860)the Jiangsu SpeciallyAppointed Professor Program,the Natural Science Foundation of Jiangsu Province(No.BK20181388)the Oversea Researcher Innovation Program of Nanjing,NUPTSF(No.NY217118)。
文摘Contact engineering is of critical importance for two-dimensional(2D)transition metal dichalcogenide(TMD)-based devices.However,there are only a few solutions to overcome this obstacle because of the complexity of the TMD-contact interface.In this work,we propose a novel method using a soft plasma treatment followed by the seamless deposition of a metal electrode to reduce the contact resistance of MoS_(2)field effect transistors(FETs).The treated FETs exhibit three times higher mobility than the control FETs without plasma treatment.The soft plasma treatment can remove the facial sulfur atoms and expose the middle Mo atoms so that they come into direct contact with the metal electrode,thus greatly improving the contact behavior.First-principles calculation is also performed to support the experimental results.Our potentially scalable strategy can be extended to the whole family of TMD based FETs to provide a possible route of device processsing technology for 2D device application.
