[Objectives]To clone the sucC gene of Vibrio alginolyticus strain HY9901 and conduct the bioinformatics analysis.[Methods]Based on the sucC gene of V.alginolyticus strain HY9901,specific primers were designed to ampli...[Objectives]To clone the sucC gene of Vibrio alginolyticus strain HY9901 and conduct the bioinformatics analysis.[Methods]Based on the sucC gene of V.alginolyticus strain HY9901,specific primers were designed to amplify the full length sequence by PCR and make further analysis.[Results]The theoretical molecular weight of SucC protein was about 41528.45 Da,and the full length was 1167 bp,encoding 388 amino acids.It has no signal peptide and transmembrane region,and has a variety of functional sites.It is predicted that it is mainly located in the cytoplasm,and the ubiquitin and lactate modification sites overlap,and it has high gene homology with Vibrio parahaemolyticus.Theα-helix,random coil and extended strand are the main secondary structures.The similarity between the constructed three-level structure model and the template is high.[Conclusions]This study reveals the structural characteristics and functional potential of SucC protein,and provides a theoretical basis for the study of drug resistance mechanism and prevention strategies.展开更多
[Objectives]This study was conducted to understand the structure and function of MsrA protein.[Methods]With Vibrio alginolyticus HY9901 as the object of study,primers were designed to amplify the full-length gene of m...[Objectives]This study was conducted to understand the structure and function of MsrA protein.[Methods]With Vibrio alginolyticus HY9901 as the object of study,primers were designed to amplify the full-length gene of msrA,and its bioinformatics analysis was carried out.[Results]The full length of msrA gene was 639 bp,encoding 212 amino acids,and its theoretical molecular weight was about 23729.60 Da.The protein had a stable structure,and it was hydrophobic overall.The structure of signal peptides at the N terminal of the amino acid sequence was predicted,and it was found that there was no signal peptide cleavage site and no transmembrane region.The amino acid sequence of MsrA contained multiple signal binding sites.Protein subcellular localization showed that MsrA protein was most likely located in the cytoplasm.Homology analysis showed that MsrA of V.alginolyticus had high homology with other Vibrio species,and the highest homology with V.alginolyticus.In the prediction of functional domains,MsrA had the function of methionine sulfoxide reduction.In secondary structure prediction,MsrA contained random coils at a proportion of 46.70%,which was the highest.The similarity between the tertiary structure model of MsrA and template Q87SW6.1.A was 89.15%.PTM analysis showed that MsrA protein had many PTM modification sites such as phosphorylation and glycosylation sites.[Conclusions]This study provides some reference value for further study on the role of MsrA in bacterial antioxidant stress.展开更多
Over the past decades, wireless technologies with reliable data transmissions and precise sensing ability in hostile environments are much less understood and significantly under-developed. The DD domain waveform, e.g...Over the past decades, wireless technologies with reliable data transmissions and precise sensing ability in hostile environments are much less understood and significantly under-developed. The DD domain waveform, e.g., orthogonal time frequency space(OTFS), has been recognized globally for its great potential to achieve high-reliable communications and robust sensing performance under various channel conditions in 6G era.展开更多
[Objectives]The paper was to clone and analyze bioinformatics of ndk gene from Vibrio alginolyticus.[Methods]A pair of specific primers was designed based on the ndk gene sequence of V.alginolyticus HY9901.The full le...[Objectives]The paper was to clone and analyze bioinformatics of ndk gene from Vibrio alginolyticus.[Methods]A pair of specific primers was designed based on the ndk gene sequence of V.alginolyticus HY9901.The full length of ndk gene was amplified by PCR and bioinformatics analysis was performed.MEGA 5.0 software was used to construct NDK phylogenetic tree by neighbor-joining method.SWISS-MODEL program was used to obtain the three-dimensional structural model of single subunit from NDK protein.[Results]The ndk gene,molecular structural formula C702H1094N192O214S7,was 426 bp in total,encoding 141 amino acids,with the theoretical molecular weight of 15.87199 kD and the theoretical pI value of 5.13.The prediction results of protein subcellular localization,SignalP 5.0,TMHMM Server 2.0 and SoftBerry-Psite showed that NDK mainly existed in the cytoplasm,and the protein was unstable and hydrophobic.There was neither signal peptide cleavage site,nor transmembrane region and KEGG metabolic pathway.The amino acid sequence had two protein kinase C phosphorylation sites,a casein kinaseⅡphosphorylation site,a N-myristoylation site,three microbody C-terminal target signal sites,and a nucleoside diphosphate kinase active site.Homology analysis showed that the NDK of V.alginolyticus had high homology with that of V.diabolicus,with a similarity of 98.58%.Analysis of the structural functional domain revealed that the protein had one NDK structural functional domain.The prediction results of secondary structure showed that theα-helix,random coil,β-sheet and extended strand accounted for 53.19%,28.37%,7.09%and 11.35%,respectively.Analysis of NDK protein via STRING database demonstrated that the proteins interacting with NDK protein were NrdA,NrdB,GmK,CmK,TmK,PyrG,PyrH,RelA,FolE and SpoT.[Conclusions]The study plays a positive role in the prevention and control of vibriosis and the improvement of the current aquaculture environment.展开更多
Joint radar and communication(JRC)technology has become important for civil and military applications for decades.This paper introduces the concepts,characteristics and advantages of JRC technology,presenting the typi...Joint radar and communication(JRC)technology has become important for civil and military applications for decades.This paper introduces the concepts,characteristics and advantages of JRC technology,presenting the typical applications that have benefited from JRC technology currently and in the future.This paper explores the state-of-the-art of JRC in the levels of coexistence,cooperation,co-design and collaboration.Compared to previous surveys,this paper reviews the entire trends that drive the development of radar sensing and wireless communication using JRC.Specifically,we explore an open research issue on radar and communication operating with mutual benefits based on collaboration,which represents the fourth stage of JRC evolution.This paper provides useful perspectives for future researches of JRC technology.展开更多
Although small cell offloading technology can alleviate the congestion in macrocell, aggressively offloading data traffic from macrocell to small cell can also degrade the performance of small cell due to the heavy lo...Although small cell offloading technology can alleviate the congestion in macrocell, aggressively offloading data traffic from macrocell to small cell can also degrade the performance of small cell due to the heavy load. Because of collision and backoff, the degradation is significant especially in network with contention-based channel access, and finally decreases throughput of the whole network. To find an optimal fraction of traffic to be offloaded in heterogeneous network, we combine Markov chain with the Poisson point process model to analyze contention-based throughput in irregularly deployment networks. Then we derive the close-form solution of the throughput and find that it is a function of the transmit power and density of base stations.Based on this, we propose the load-aware offloading strategies via power control and base station density adjustment. The numerical results verify our analysis and show a great performance gain compared with non-load-aware offloading.展开更多
[Objectives]The crp gene in Vibrio alginolyticus was studied.[Methods]The crp gene of V.alginolyticus HY9901 was cloned and analyzed by bioinformatics.[Results]The ORF of the crp gene is 633 bp long and the predicted ...[Objectives]The crp gene in Vibrio alginolyticus was studied.[Methods]The crp gene of V.alginolyticus HY9901 was cloned and analyzed by bioinformatics.[Results]The ORF of the crp gene is 633 bp long and the predicted amino acid sequence encompasses 210 amino acid residues.The physicochemical property analysis indicated that the chemical formula of CRP is C_(1051)H_(1704)N_(290)O_(308)S_(10) with a molecular weight of 23.6514 kDa,and its theoretical pI is 7.74.Besides,the protein is stable and hydrophilic.The protein had three protein kinase C phosphorylation site,three casein kinase II phosphorylation site,one N-terminal myristoylation site.The BLAST analysis on the sequence revealed high homology with CRPs in other Vibrio species,and particularly the sequence shares about a homology of 99.52%with the CRP in V.parahaemolyticus.The SWISS-MODEL software simulated the subunit tertiary structural model of the CRP,and the similarity with template 3hif.1.A was 95.71%.[Conclusions]This study provides a reference for the search for efficient protective antigens against vibrosis.展开更多
The integration of communications,sensing and computing(I-CSC)has significant applications in vehicular ad hoc networks(VANETs).A roadside unit(RSU)plays an important role in I-CSC by performing functions such as info...The integration of communications,sensing and computing(I-CSC)has significant applications in vehicular ad hoc networks(VANETs).A roadside unit(RSU)plays an important role in I-CSC by performing functions such as information transmission and edge computing in vehicular communication.Due to the constraints of limited resources,RSU cannot achieve full coverage and deploying RSUs at key cluster heads of hierarchical structures of road networks is an effective management method.However,direct extracting the hierarchical structures for the resource allocation in VANETs is an open issue.In this paper,we proposed a network-based renormalization method based on information flow and geographical location to hierarchically deploy the RSU on the road networks.The renormalization method is compared with two deployment schemes:genetic algorithm(GA)and memetic framework-based optimal RSU deployment(MFRD),to verify the improvement of communication performance.Our results show that the renormalization method is superior to other schemes in terms of RSU coverage and information reception rate.展开更多
Considering the dynamic changes and uncertainty features of the radio environment in cognitive wireless networks(CWNs),the environment cognition ability is critical for the performance evaluation of CWNs design and op...Considering the dynamic changes and uncertainty features of the radio environment in cognitive wireless networks(CWNs),the environment cognition ability is critical for the performance evaluation of CWNs design and optimization.However,there are no effective metrics to evaluate the ability and gain of information cognition in CWNs from an information theory perspective.Therefore,the novel cognitive information concept is proposed and defined as a metric to evaluate the uncertainty of both the internal and external environments of one system that can be removed by other systems or nodes using cognitive radio techniques.As an intelligent wireless communication system that is aware of its surrounding radio,network,and user multi-domains environment,the more cognitive information it achieves,the higher level cognitive capability it is.In this paper,we define and analyze the mathematical features of cognitive information.Results reveal that the increase of cognitive information can improve the spectrum efficiency and reduce the interference probability simultaneously in CWNs.Thus cognitive information can be regarded as a metric for CWNs optimization.Finally,we apply the theory of cognitive information in the parameters optimization in energy detection and cooperative spectrum sensing.展开更多
基金Supported by National Natural Science Foundation of China(32073015)Graduate Education Innovation Program of Guangdong Province(YJYH[2022]1)+1 种基金Undergraduate Innovation and Entrepreneurship Training Program of Guangdong Ocean University(CXXL2024007)Undergraduate Innovation Team of Guangdong Ocean University(CCTD201802).
文摘[Objectives]To clone the sucC gene of Vibrio alginolyticus strain HY9901 and conduct the bioinformatics analysis.[Methods]Based on the sucC gene of V.alginolyticus strain HY9901,specific primers were designed to amplify the full length sequence by PCR and make further analysis.[Results]The theoretical molecular weight of SucC protein was about 41528.45 Da,and the full length was 1167 bp,encoding 388 amino acids.It has no signal peptide and transmembrane region,and has a variety of functional sites.It is predicted that it is mainly located in the cytoplasm,and the ubiquitin and lactate modification sites overlap,and it has high gene homology with Vibrio parahaemolyticus.Theα-helix,random coil and extended strand are the main secondary structures.The similarity between the constructed three-level structure model and the template is high.[Conclusions]This study reveals the structural characteristics and functional potential of SucC protein,and provides a theoretical basis for the study of drug resistance mechanism and prevention strategies.
基金Supported National Natural Science Foundation of China(32073015)Undergraduate Training Program for Innovation and Entrepreneurship of Guangdong Ocean University(CXXL2024007)+2 种基金Undergraduate Innovation Team of Guangdong Ocean University(CCTD201802)Postgraduate Education Innovation Program of Guangdong Ocean University(No.202446)Postgraduate Education Innovation Program of Guangdong Province(YJYH[2022]1).
文摘[Objectives]This study was conducted to understand the structure and function of MsrA protein.[Methods]With Vibrio alginolyticus HY9901 as the object of study,primers were designed to amplify the full-length gene of msrA,and its bioinformatics analysis was carried out.[Results]The full length of msrA gene was 639 bp,encoding 212 amino acids,and its theoretical molecular weight was about 23729.60 Da.The protein had a stable structure,and it was hydrophobic overall.The structure of signal peptides at the N terminal of the amino acid sequence was predicted,and it was found that there was no signal peptide cleavage site and no transmembrane region.The amino acid sequence of MsrA contained multiple signal binding sites.Protein subcellular localization showed that MsrA protein was most likely located in the cytoplasm.Homology analysis showed that MsrA of V.alginolyticus had high homology with other Vibrio species,and the highest homology with V.alginolyticus.In the prediction of functional domains,MsrA had the function of methionine sulfoxide reduction.In secondary structure prediction,MsrA contained random coils at a proportion of 46.70%,which was the highest.The similarity between the tertiary structure model of MsrA and template Q87SW6.1.A was 89.15%.PTM analysis showed that MsrA protein had many PTM modification sites such as phosphorylation and glycosylation sites.[Conclusions]This study provides some reference value for further study on the role of MsrA in bacterial antioxidant stress.
文摘Over the past decades, wireless technologies with reliable data transmissions and precise sensing ability in hostile environments are much less understood and significantly under-developed. The DD domain waveform, e.g., orthogonal time frequency space(OTFS), has been recognized globally for its great potential to achieve high-reliable communications and robust sensing performance under various channel conditions in 6G era.
基金Supported by Outstanding Graduate Entering Laboratory Project of College of Fisheries,Guangdong Ocean UniversityNational Natural Science Foundationof China(32073015)+2 种基金Natural Science Foundation of Guangdong Province(2021A1515011078)Undergraduate Innovation and Entrepreneurship Train-ing Program of Guangdong Ocean University(CXXL2022005)UndergraduateInnovation Team of Guangdong Ocean University(CCTD201802)。
文摘[Objectives]The paper was to clone and analyze bioinformatics of ndk gene from Vibrio alginolyticus.[Methods]A pair of specific primers was designed based on the ndk gene sequence of V.alginolyticus HY9901.The full length of ndk gene was amplified by PCR and bioinformatics analysis was performed.MEGA 5.0 software was used to construct NDK phylogenetic tree by neighbor-joining method.SWISS-MODEL program was used to obtain the three-dimensional structural model of single subunit from NDK protein.[Results]The ndk gene,molecular structural formula C702H1094N192O214S7,was 426 bp in total,encoding 141 amino acids,with the theoretical molecular weight of 15.87199 kD and the theoretical pI value of 5.13.The prediction results of protein subcellular localization,SignalP 5.0,TMHMM Server 2.0 and SoftBerry-Psite showed that NDK mainly existed in the cytoplasm,and the protein was unstable and hydrophobic.There was neither signal peptide cleavage site,nor transmembrane region and KEGG metabolic pathway.The amino acid sequence had two protein kinase C phosphorylation sites,a casein kinaseⅡphosphorylation site,a N-myristoylation site,three microbody C-terminal target signal sites,and a nucleoside diphosphate kinase active site.Homology analysis showed that the NDK of V.alginolyticus had high homology with that of V.diabolicus,with a similarity of 98.58%.Analysis of the structural functional domain revealed that the protein had one NDK structural functional domain.The prediction results of secondary structure showed that theα-helix,random coil,β-sheet and extended strand accounted for 53.19%,28.37%,7.09%and 11.35%,respectively.Analysis of NDK protein via STRING database demonstrated that the proteins interacting with NDK protein were NrdA,NrdB,GmK,CmK,TmK,PyrG,PyrH,RelA,FolE and SpoT.[Conclusions]The study plays a positive role in the prevention and control of vibriosis and the improvement of the current aquaculture environment.
基金supported by the National Natural Science Foundation of China (No. 61631003, 61601055)the National Science Fund for Distinguished Young Scholars (No. 61525101)
文摘Joint radar and communication(JRC)technology has become important for civil and military applications for decades.This paper introduces the concepts,characteristics and advantages of JRC technology,presenting the typical applications that have benefited from JRC technology currently and in the future.This paper explores the state-of-the-art of JRC in the levels of coexistence,cooperation,co-design and collaboration.Compared to previous surveys,this paper reviews the entire trends that drive the development of radar sensing and wireless communication using JRC.Specifically,we explore an open research issue on radar and communication operating with mutual benefits based on collaboration,which represents the fourth stage of JRC evolution.This paper provides useful perspectives for future researches of JRC technology.
基金supported by the National High-Tech R&D Program (863 Program) under grant No. 2015AA01A705Beijing Municipal Science and Technology Commission research fund project under grant No. D151100000115002+1 种基金China Scholarship Council under grant No. 201406470038BUPT youth scientific research innovation program under grant No. 500401238
文摘Although small cell offloading technology can alleviate the congestion in macrocell, aggressively offloading data traffic from macrocell to small cell can also degrade the performance of small cell due to the heavy load. Because of collision and backoff, the degradation is significant especially in network with contention-based channel access, and finally decreases throughput of the whole network. To find an optimal fraction of traffic to be offloaded in heterogeneous network, we combine Markov chain with the Poisson point process model to analyze contention-based throughput in irregularly deployment networks. Then we derive the close-form solution of the throughput and find that it is a function of the transmit power and density of base stations.Based on this, we propose the load-aware offloading strategies via power control and base station density adjustment. The numerical results verify our analysis and show a great performance gain compared with non-load-aware offloading.
基金Supported by Project of Outstanding Undergraduates Entering Laboratory of Fisheries College,Guangdong Ocean UniversityNational Natural Science Foundation of China(32073015)+3 种基金Natural Science Foundation of Guangdong Province(2021A1515011078)Special Fund for Science and Technology Innovation Strategy in Guangdong Province(Undergraduates Science and Technology Innovation Cultivation)(pdjh2021b0239)Undergraduate Innovation and Enterpreneurship Training Program of Guangdong Ocean University(CXXL2021122)Undergraduate Innovation Team of Guangdong Ocean University(CCTD201802).
文摘[Objectives]The crp gene in Vibrio alginolyticus was studied.[Methods]The crp gene of V.alginolyticus HY9901 was cloned and analyzed by bioinformatics.[Results]The ORF of the crp gene is 633 bp long and the predicted amino acid sequence encompasses 210 amino acid residues.The physicochemical property analysis indicated that the chemical formula of CRP is C_(1051)H_(1704)N_(290)O_(308)S_(10) with a molecular weight of 23.6514 kDa,and its theoretical pI is 7.74.Besides,the protein is stable and hydrophilic.The protein had three protein kinase C phosphorylation site,three casein kinase II phosphorylation site,one N-terminal myristoylation site.The BLAST analysis on the sequence revealed high homology with CRPs in other Vibrio species,and particularly the sequence shares about a homology of 99.52%with the CRP in V.parahaemolyticus.The SWISS-MODEL software simulated the subunit tertiary structural model of the CRP,and the similarity with template 3hif.1.A was 95.71%.[Conclusions]This study provides a reference for the search for efficient protective antigens against vibrosis.
文摘The integration of communications,sensing and computing(I-CSC)has significant applications in vehicular ad hoc networks(VANETs).A roadside unit(RSU)plays an important role in I-CSC by performing functions such as information transmission and edge computing in vehicular communication.Due to the constraints of limited resources,RSU cannot achieve full coverage and deploying RSUs at key cluster heads of hierarchical structures of road networks is an effective management method.However,direct extracting the hierarchical structures for the resource allocation in VANETs is an open issue.In this paper,we proposed a network-based renormalization method based on information flow and geographical location to hierarchically deploy the RSU on the road networks.The renormalization method is compared with two deployment schemes:genetic algorithm(GA)and memetic framework-based optimal RSU deployment(MFRD),to verify the improvement of communication performance.Our results show that the renormalization method is superior to other schemes in terms of RSU coverage and information reception rate.
基金supported by the National Natural Science Foundation of China (61227801,61201152,61121001)the Major State Basic Research Development Program of China (973 Program,2009CB320400)+2 种基金the National Science and Technology Major Project (2012ZX03003006)the Program for New Century Excellent Talents in University (NCET-01-0259)the Fundamental Research Funds for the Central Universities (2013RC0106)
文摘Considering the dynamic changes and uncertainty features of the radio environment in cognitive wireless networks(CWNs),the environment cognition ability is critical for the performance evaluation of CWNs design and optimization.However,there are no effective metrics to evaluate the ability and gain of information cognition in CWNs from an information theory perspective.Therefore,the novel cognitive information concept is proposed and defined as a metric to evaluate the uncertainty of both the internal and external environments of one system that can be removed by other systems or nodes using cognitive radio techniques.As an intelligent wireless communication system that is aware of its surrounding radio,network,and user multi-domains environment,the more cognitive information it achieves,the higher level cognitive capability it is.In this paper,we define and analyze the mathematical features of cognitive information.Results reveal that the increase of cognitive information can improve the spectrum efficiency and reduce the interference probability simultaneously in CWNs.Thus cognitive information can be regarded as a metric for CWNs optimization.Finally,we apply the theory of cognitive information in the parameters optimization in energy detection and cooperative spectrum sensing.
