Herein,a novel interference-free surface-enhanced Raman spectroscopy(SERS)strategy based on magnetic nanoparticles(MNPs)and aptamer-driven assemblies was proposed for the ultrasensitive detection of histamine.A core-s...Herein,a novel interference-free surface-enhanced Raman spectroscopy(SERS)strategy based on magnetic nanoparticles(MNPs)and aptamer-driven assemblies was proposed for the ultrasensitive detection of histamine.A core-satellite SERS aptasensor was constructed by combining aptamer-decorated Fe_(3)O_(4)@Au MNPs(as the recognize probe for histamine)and complementary DNA-modified silver nanoparticles carrying 4-mercaptobenzonitrile(4-MBN)(Ag@4-MBN@Ag-c-DNA)as the SERS signal probe for the indirect detection of histamine.Under an applied magnetic field in the absence of histamine,the assembly gave an intense Raman signal at“Raman biological-silent”region due to 4-MBN.In the presence of histamine,the Ag@4-MBN@Ag-c-DNA SERS-tag was released from the Fe_(3)O_(4)@Au MNPs,thus decreasing the SERS signal.Under optimal conditions,an ultra-low limit of detection of 0.65×10^(-3)ng/mL and a linear range 10^(-2)-10^5 ng/mL on the SERS aptasensor were obtained.The histamine content in four food samples were analyzed using the SERS aptasensor,with the results consistent with those determined by high performance liquid chromatography.The present work highlights the merits of indirect strategies for the ultrasensitive and highly selective SERS detection of small biological molecules in complex matrices.展开更多
3-Epi-betulinic acid 3-O-β-D-glucopyranoside(eBAG)is a pentacyclic triterpene mainly distributed in food and medicinal plants,which exhibits various pharmacological properties.However,whether these functions are attr...3-Epi-betulinic acid 3-O-β-D-glucopyranoside(eBAG)is a pentacyclic triterpene mainly distributed in food and medicinal plants,which exhibits various pharmacological properties.However,whether these functions are attributed to eBAG or additional components in these plants remain unknown.Herein,we report that eBAG exerted an inhibitory activity against hepatocellular carcinoma and esophageal cancer cells.EBAG induced non-apoptotic cell death in hepatocellular carcinoma cells.The eBAG-induced cell death was inhibited by knock-down of autophagy related gene(ATG)5 and ATG7,by administration of 3-methyladenine,a selective autophagy inhibitor that suppresses phosphoinositide 3-kinase(PI3K),and by chloroquine,a classic autophagy flux inhibitor.We demonstrated that eBAG induced an autophagy-mediated cell death.Application of eBAG mimicked cellular bioenergetics depletion leading to the reduction of intracellular ATP,activation of AMP-activated protein kinase(AMPK),and inhibition of mTOR.Co-treatment with compound C,an AMPK inhibitor,abrogated cell death induced by eBAG.We further validated the anti-tumor effect of eBAG in the murine xenograft model of hepatocellular carcinoma and found that eBAG treatment promoted the induction of autophagy and reduction of tumor growth in mice.As a functional food ingredient,eBAG is a potential therapeutic agent for the treatment of hepatocellular carcinoma and esophageal cancer.展开更多
A new method of pretreatment of corn straw with Phanerochaete chrysosporium combined with pyrolysis was proposed to improve the quality of bio-oil.The characterization results demonstrated that microbial pretreatment ...A new method of pretreatment of corn straw with Phanerochaete chrysosporium combined with pyrolysis was proposed to improve the quality of bio-oil.The characterization results demonstrated that microbial pretreatment was an effective method to decrease the lignin,which can achieve a maximum removal rate of 44.19%.Due to the destruction of biomass structure,the content of alkali metal and alkaline earth metal is reduced.Meanwhile,the depolymerized biomass structure created better pyrolysis conditions to promote the pyrolysis efficiency,increase the average decomposition rate of pyrolysis and reduce the residue.In fast pyrolysis,because of the enrichment of cellulose and the removal of lignin,the contents of acids,linear carbonyls,furans,and sugars increased while the contents of phenols decreased.As for the catalytic pyrolysis,due to the hydrocarbon pool reaction and shape selection deoxidation of ZSM-5 catalyst,the total hydrocarbon and aromatics contents can significantly increase up to 34.37%and 30.59%,respectively,with 3 weeks of pretreatment.And the the low molecular content of bio-oil increased after pretreatment,which can significantly benefit the entry of primary pyrolysis steam into the catalyst pores to improve the catalytic efficiency and hydrocarbon contents.This method provides a new treatment idea for high-quality utilization of biomass.展开更多
Promoting the use of ethanol gasoline is an important approach for China to achieve sustainable and high quality development.Based on the daily air quality data of Tianjin,China,from 2013 to 2020,this paper investigat...Promoting the use of ethanol gasoline is an important approach for China to achieve sustainable and high quality development.Based on the daily air quality data of Tianjin,China,from 2013 to 2020,this paper investigates whether the implementation of the ethanol gasoline policy helped improve air quality using regression discontinuity analysis.We find that the implementation of the ethanol gasoline policy had a very limited positive effect on the overall air quality index(AQI)and no obvious effect on the reduction of particulate matter(PM_(2.5) and PM_(10)).However,the results reveal that the ethanol gasoline policy reduced SO_(2),NO_(2),and CO by about 36%,19%,and 20%,respectively.These findings provide policy insights for other cities in China and other rapidly developing cities in the world to promote the use of ethanol gasoline.展开更多
Dear Editor,Chikungunya virus(CHIKV)is a mosquito-borne virus belonging to the family Togaviridae,genus Alphavirus,and was first isolated in Tanzania in the 1950s(Silva and Dermody 2017;Weaver and Lecuit 2015).Human i...Dear Editor,Chikungunya virus(CHIKV)is a mosquito-borne virus belonging to the family Togaviridae,genus Alphavirus,and was first isolated in Tanzania in the 1950s(Silva and Dermody 2017;Weaver and Lecuit 2015).Human infections with CHIKV typically result in a rapid-onset febrile disease,with symptoms that include fever,headache,rash,severe joint and muscle pain,as well as prolonged periods of disability in some patients(Weaver and Lecuit 2015;Silva and Dermody 2017).展开更多
Summary What is already known about this topic?The World Health Organization consolidated guidelines on recommend care for tuberculosis(TB)and support for multidrug-resistant TB(MDR-TB)patients.But guidelines have not...Summary What is already known about this topic?The World Health Organization consolidated guidelines on recommend care for tuberculosis(TB)and support for multidrug-resistant TB(MDR-TB)patients.But guidelines have not provided detailed guidance or tools for health services providers to implement comprehensive patient care.展开更多
基金financially supported by the National Natural Science Foundation of China(31972149)funding support from the MacDiarmid Institute for Advanced Materials and Nanotechnologythe Dodd-Walls Centre for Photonic and Quantum Technologies。
文摘Herein,a novel interference-free surface-enhanced Raman spectroscopy(SERS)strategy based on magnetic nanoparticles(MNPs)and aptamer-driven assemblies was proposed for the ultrasensitive detection of histamine.A core-satellite SERS aptasensor was constructed by combining aptamer-decorated Fe_(3)O_(4)@Au MNPs(as the recognize probe for histamine)and complementary DNA-modified silver nanoparticles carrying 4-mercaptobenzonitrile(4-MBN)(Ag@4-MBN@Ag-c-DNA)as the SERS signal probe for the indirect detection of histamine.Under an applied magnetic field in the absence of histamine,the assembly gave an intense Raman signal at“Raman biological-silent”region due to 4-MBN.In the presence of histamine,the Ag@4-MBN@Ag-c-DNA SERS-tag was released from the Fe_(3)O_(4)@Au MNPs,thus decreasing the SERS signal.Under optimal conditions,an ultra-low limit of detection of 0.65×10^(-3)ng/mL and a linear range 10^(-2)-10^5 ng/mL on the SERS aptasensor were obtained.The histamine content in four food samples were analyzed using the SERS aptasensor,with the results consistent with those determined by high performance liquid chromatography.The present work highlights the merits of indirect strategies for the ultrasensitive and highly selective SERS detection of small biological molecules in complex matrices.
基金supported by Henan Provincial Science and Technology Research Project (212102310355)the National Natural Science Foundation of China (82020108024 and 32161143021)。
文摘3-Epi-betulinic acid 3-O-β-D-glucopyranoside(eBAG)is a pentacyclic triterpene mainly distributed in food and medicinal plants,which exhibits various pharmacological properties.However,whether these functions are attributed to eBAG or additional components in these plants remain unknown.Herein,we report that eBAG exerted an inhibitory activity against hepatocellular carcinoma and esophageal cancer cells.EBAG induced non-apoptotic cell death in hepatocellular carcinoma cells.The eBAG-induced cell death was inhibited by knock-down of autophagy related gene(ATG)5 and ATG7,by administration of 3-methyladenine,a selective autophagy inhibitor that suppresses phosphoinositide 3-kinase(PI3K),and by chloroquine,a classic autophagy flux inhibitor.We demonstrated that eBAG induced an autophagy-mediated cell death.Application of eBAG mimicked cellular bioenergetics depletion leading to the reduction of intracellular ATP,activation of AMP-activated protein kinase(AMPK),and inhibition of mTOR.Co-treatment with compound C,an AMPK inhibitor,abrogated cell death induced by eBAG.We further validated the anti-tumor effect of eBAG in the murine xenograft model of hepatocellular carcinoma and found that eBAG treatment promoted the induction of autophagy and reduction of tumor growth in mice.As a functional food ingredient,eBAG is a potential therapeutic agent for the treatment of hepatocellular carcinoma and esophageal cancer.
基金supported by the Natural Science Fund Program of China (No.52306043)the Science and Technology Project of Jiangsu Province (Nos.BE2022604&BE2021701)+2 种基金the Youth Talent Promotion Project of Jiangsu Provincial Association for Science and Technology (No.JSTJ-2022-046)the Inner Mongolia Major Science and Technology Project (No.2021ZD0022)the State Key Laboratory of High-Efficiency Utilization of Coal and Green Chemical Engineering (China) (Nos.2022-K25&2022-K37).
文摘A new method of pretreatment of corn straw with Phanerochaete chrysosporium combined with pyrolysis was proposed to improve the quality of bio-oil.The characterization results demonstrated that microbial pretreatment was an effective method to decrease the lignin,which can achieve a maximum removal rate of 44.19%.Due to the destruction of biomass structure,the content of alkali metal and alkaline earth metal is reduced.Meanwhile,the depolymerized biomass structure created better pyrolysis conditions to promote the pyrolysis efficiency,increase the average decomposition rate of pyrolysis and reduce the residue.In fast pyrolysis,because of the enrichment of cellulose and the removal of lignin,the contents of acids,linear carbonyls,furans,and sugars increased while the contents of phenols decreased.As for the catalytic pyrolysis,due to the hydrocarbon pool reaction and shape selection deoxidation of ZSM-5 catalyst,the total hydrocarbon and aromatics contents can significantly increase up to 34.37%and 30.59%,respectively,with 3 weeks of pretreatment.And the the low molecular content of bio-oil increased after pretreatment,which can significantly benefit the entry of primary pyrolysis steam into the catalyst pores to improve the catalytic efficiency and hydrocarbon contents.This method provides a new treatment idea for high-quality utilization of biomass.
文摘Promoting the use of ethanol gasoline is an important approach for China to achieve sustainable and high quality development.Based on the daily air quality data of Tianjin,China,from 2013 to 2020,this paper investigates whether the implementation of the ethanol gasoline policy helped improve air quality using regression discontinuity analysis.We find that the implementation of the ethanol gasoline policy had a very limited positive effect on the overall air quality index(AQI)and no obvious effect on the reduction of particulate matter(PM_(2.5) and PM_(10)).However,the results reveal that the ethanol gasoline policy reduced SO_(2),NO_(2),and CO by about 36%,19%,and 20%,respectively.These findings provide policy insights for other cities in China and other rapidly developing cities in the world to promote the use of ethanol gasoline.
基金the grants from the National Natural Science Foundation(NSFC)of China(81802004)the Shenzhen Science and Technology Research and Development Project(JCYJ20160427153238750)+2 种基金the National Science and Technology Major Project(2018ZX10711001,2017ZX10103011)Strategic Priority Research Program of the Chinese Academy of Sciences(CAS)(XDB29010102)the Sanming Project of Medicine in Shenzhen(SZSM201412003).Y.B.is supported by the NSFC Outstanding Young Scholars(31822055).
文摘Dear Editor,Chikungunya virus(CHIKV)is a mosquito-borne virus belonging to the family Togaviridae,genus Alphavirus,and was first isolated in Tanzania in the 1950s(Silva and Dermody 2017;Weaver and Lecuit 2015).Human infections with CHIKV typically result in a rapid-onset febrile disease,with symptoms that include fever,headache,rash,severe joint and muscle pain,as well as prolonged periods of disability in some patients(Weaver and Lecuit 2015;Silva and Dermody 2017).
文摘Summary What is already known about this topic?The World Health Organization consolidated guidelines on recommend care for tuberculosis(TB)and support for multidrug-resistant TB(MDR-TB)patients.But guidelines have not provided detailed guidance or tools for health services providers to implement comprehensive patient care.
