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Puccinia triticina effector protein Pt_21 interacts with wheat thaumatin-like protein TaTLP1 to inhibit its antifungal activity and suppress wheat apoplast immunity 被引量:2
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作者 Fei Wang Songsong Shen +8 位作者 zhongchi cui Shitao Yuan Ping Qu Hui Jia Linshuo Meng Xiaoyu Hao Daqun Liu Lisong Ma Haiyan Wang 《The Crop Journal》 SCIE CSCD 2023年第5期1431-1440,共10页
Puccinia triticina(Pt), as the causal agent of wheat leaf rust, employs a plethora of effector proteins to modulate wheat immunity for successful colonization. Understanding the molecular mechanisms underlying Pt effe... Puccinia triticina(Pt), as the causal agent of wheat leaf rust, employs a plethora of effector proteins to modulate wheat immunity for successful colonization. Understanding the molecular mechanisms underlying Pt effector-mediated wheat susceptibility remains largely unexplored. In this study, an effector Pt_21 was identified to interact with the apoplast-localized wheat thaumatin-like protein TaTLP1 using a yeast two-hybrid assay and the Pt_21-TaTLP1 interaction was characterized. The interaction between Pt_21 and TaTLP1 was validated by in vivo co-immunoprecipitation assay. A TaTLP1 variant,TaTLP1C71A, that was identified by the site-directed mutagenesis failed to interact with Pt_21. Pt_21was able to suppress Bax-mediated cell death in leaves of Nicotiana benthamiana and inhibit TaTLP1-mediated antifungal activity. Furthermore, infiltration of recombinant protein Pt_21 into leaves of transgenic wheat line overexpressing TaTLP1 enhanced the disease development of leaf rust compared to that in wild-type leaves. These findings demonstrate that Pt_21 suppresses host defense response by directly targeting wheat TaTLP1 and inhibiting its antifungal activity, which broadens our understanding of the molecular mechanisms underlying Pt effector-mediated susceptibility in wheat. 展开更多
关键词 WHEAT Puccinia triticina EFFECTOR Thaumatin-like protein Anitifungal activity
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Transgenic expression of TaTLP1, a thaumatin-like protein gene, reduces susceptibility to common root rot and leaf rust in wheat 被引量:3
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作者 zhongchi cui Fang Liang +3 位作者 Jiarui Zhang Fei Wang Daqun Liu Haiyan Wang 《The Crop Journal》 SCIE CSCD 2021年第5期1214-1218,共5页
Thaumatin-like protein (TLP) plays an important role in combating plant pathogen infection.Common root rot caused by Bipolaris sorokiniana and leaf rust caused by Puccinia triticina (Pt) are major fungal diseases in w... Thaumatin-like protein (TLP) plays an important role in combating plant pathogen infection.Common root rot caused by Bipolaris sorokiniana and leaf rust caused by Puccinia triticina (Pt) are major fungal diseases in wheat.The disease responses of TaTLP1-overexpressing transgenic lines (TaTLP1-OE) were evaluated after inoculation with each pathogen.The TaTLP1-OE lines had no apparent differences in tiller number and 1000-kernel weight from the wild type Jinan Wheat No.1 (JW1),whereas resistance to leaf rust and common root rot was improved,resulting from activated peroxidase and b-1,3-glucanase after B.sorokiniana infection,and reactive oxygen species-related genes were upregulated in Ta TLP1-OE lines after Pt infection.These results indicated that stable expression of TaTLP1 increased resistance against both diseases. 展开更多
关键词 Bipolaris sorokiniana Disease resistance Puccinia triticina Transgenic wheat
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