BACKGROUND： Nikethamide, a respiratory center stimulant, is widely used in China. However, its effects on the central nervous system and medullary respiratory center remain poorly understood. OBJECTIVE： To investiga...BACKGROUND： Nikethamide, a respiratory center stimulant, is widely used in China. However, its effects on the central nervous system and medullary respiratory center remain poorly understood. OBJECTIVE： To investigate the influence of nikethamide on inspiratory neuron discharge in the medial region of the nucleus retrofacialis in neonatal rats, based on the observations addressing rhythmic respiratory discharge generated by the basic medullary respiratory center and various respiration neuron discharges in brain slices. DESIGN, TIME AND SETTING： A controlled, observational study utilizing in vitro neuroelectrophysiology was performed at the Department of Physiology in Southern Medical University between September and December in 2007. MATERIALS： Nikethamide was purchased from Sigma, USA; BL-420E biological signal collection and manaclement system was provided by Chengdu TME Technology, China.METHODS： Isolated medulla-spinal cord preparations were collected from neonatal Sprague Dawley rats, aged 1-3 days. Tissues were divided to include the medial region of the nucleus retrofacialis, ventral respiratory, and dorsal respiratory groups. Subsequently, modified Kreb＇s solution and 5 μg/mL nikethamide-containing modified Kreb＇s solution were consecutively perfused into the medial region of the nucleus retrofacialis in neonatal rat brain slices. MAIN OUTCOME MEASURES： Hypoglossal nerve root respiratory-related rhythmic discharge activities and inspiratory neuron discharges were recorded with an adsorption electrode and microelectrode. RESULTS Nikethamide resulted in prolonged inspiratory neuron discharge time, shortened respiratory cycle and expiratory time. Nikethamide intervention resulted in enhanced integral amplitude of some inspiratory neurons with no changes in discharge frequency or increased discharge frequency in remaining inspiratory neurons with no changes in integral amplitude. CONCLUSION： Nikethamide excites inspiratory neurons in the basic rhythmic respiration and medullary respiratory center, in addition to increased inspiratory neuron and neural network excitability.展开更多
In order to improve the efficiency of elderly evaluation, an optimization method based on rough set is proposed. Compared with the traditional rough set attribute reduction, the redundant evaluation items are eliminat...In order to improve the efficiency of elderly evaluation, an optimization method based on rough set is proposed. Compared with the traditional rough set attribute reduction, the redundant evaluation items are eliminated by items’ correlation. It avoids a big overhead of calculating the core of rough sets that have many attributes. A novel rule reduction method is proposed based on reliability and coverage, in order to solve the problem of rarely appeared rules and conflict rules in traditional rough set. A sorting algorithm based on coverage is used to optimize the traditional flat evaluation questionnaire model with a hierarchical order. By these optimizations, the number of items that need to evaluate is greatly reduced. The proposed approach is deployed in an elderly service company named Lime family. Real-life result shows that the method can reduce more than 40% items with over 90% accuracy prediction rate. Compared with decision tree and the method based on expert knowledge in reduction rate and accuracy rate, the method has same performance in one index, and 20% improvement on average in the other one.展开更多
基金the National Natural Science Foundation of China,No.30570670the Natural Science Foundation of Guangdong Province,No.5004714
文摘BACKGROUND： Nikethamide, a respiratory center stimulant, is widely used in China. However, its effects on the central nervous system and medullary respiratory center remain poorly understood. OBJECTIVE： To investigate the influence of nikethamide on inspiratory neuron discharge in the medial region of the nucleus retrofacialis in neonatal rats, based on the observations addressing rhythmic respiratory discharge generated by the basic medullary respiratory center and various respiration neuron discharges in brain slices. DESIGN, TIME AND SETTING： A controlled, observational study utilizing in vitro neuroelectrophysiology was performed at the Department of Physiology in Southern Medical University between September and December in 2007. MATERIALS： Nikethamide was purchased from Sigma, USA; BL-420E biological signal collection and manaclement system was provided by Chengdu TME Technology, China.METHODS： Isolated medulla-spinal cord preparations were collected from neonatal Sprague Dawley rats, aged 1-3 days. Tissues were divided to include the medial region of the nucleus retrofacialis, ventral respiratory, and dorsal respiratory groups. Subsequently, modified Kreb＇s solution and 5 μg/mL nikethamide-containing modified Kreb＇s solution were consecutively perfused into the medial region of the nucleus retrofacialis in neonatal rat brain slices. MAIN OUTCOME MEASURES： Hypoglossal nerve root respiratory-related rhythmic discharge activities and inspiratory neuron discharges were recorded with an adsorption electrode and microelectrode. RESULTS Nikethamide resulted in prolonged inspiratory neuron discharge time, shortened respiratory cycle and expiratory time. Nikethamide intervention resulted in enhanced integral amplitude of some inspiratory neurons with no changes in discharge frequency or increased discharge frequency in remaining inspiratory neurons with no changes in integral amplitude. CONCLUSION： Nikethamide excites inspiratory neurons in the basic rhythmic respiration and medullary respiratory center, in addition to increased inspiratory neuron and neural network excitability.
文摘In order to improve the efficiency of elderly evaluation, an optimization method based on rough set is proposed. Compared with the traditional rough set attribute reduction, the redundant evaluation items are eliminated by items’ correlation. It avoids a big overhead of calculating the core of rough sets that have many attributes. A novel rule reduction method is proposed based on reliability and coverage, in order to solve the problem of rarely appeared rules and conflict rules in traditional rough set. A sorting algorithm based on coverage is used to optimize the traditional flat evaluation questionnaire model with a hierarchical order. By these optimizations, the number of items that need to evaluate is greatly reduced. The proposed approach is deployed in an elderly service company named Lime family. Real-life result shows that the method can reduce more than 40% items with over 90% accuracy prediction rate. Compared with decision tree and the method based on expert knowledge in reduction rate and accuracy rate, the method has same performance in one index, and 20% improvement on average in the other one.
