Objective: Exploring the therapeutic effects of acupuncture for male immune infertility using Meta-analysis. Methods: The literature related to clinical randomized controlled trials (RCTs) on acupuncture for male immu...Objective: Exploring the therapeutic effects of acupuncture for male immune infertility using Meta-analysis. Methods: The literature related to clinical randomized controlled trials (RCTs) on acupuncture for male immune infertility published from the establishment of the database (journal) to 2021 was searched for RR values or OR values and 95% CI as effect indicators. RevMan 5.3 software was applied for meta-analysis. Results: Acupuncture or combination of acupuncture and herbal medicine (hereafter referred to as acupuncture and medicine) or electro-acupuncture, the total effective rate was significantly better than the control group, and the difference was statistically significant [RR = 1.29, 95% CI (1.20, 1.38), p 0.00001];In addition, the efficiency of the combined acupuncture and medicine treatment was better than that of the herbal medicine group alone, and the difference was statistically significant [RR = 1.05, 95%, CI (0.94, 1.16), P = 0.42];The sperm viability in the combined acupuncture and medicine treatment group was significantly better than that in the herbal medicine treatment group alone, and the differences were all statistically significant [MD = 0.04, 95% CI (?0.20, 0.28), P0.74];Sperm forward motion was significantly better in the combination of acupuncture and medicine than in the herbal medicine alone group, and the differences were all statistically significant [MD = 0.66, 95% CI (?0.04, 1.36), P = 0.06];ACP indexes were significantly higher in the combination of acupuncture and medicine than in the herbal medicine alone group, with a statistically significant difference [MD = 20.47, 95% CI (?65.31, 106.25), P = 0.64];The AsAb content in the seminal plasma of either needle medicine or acupuncture was lower than in the homogeneous prednisone group, and the difference was statistically significant [MD = ?7.00, 95% CI (?11.19, ?2.81), P = 0.001];The index of AsAb content in the serum of either needle medicine or acupuncture was lower than that of prednisone group, and the difference was statistically significant [MD = ?5.00, 95% CI (?9.53, ?0.47), P = 0.03]. Conclusion: Based on current evidence, acupuncture is more effective than Western medicine (prednisone) alone in the treatment of male immune infertility, and is more effective when combined with Chinese medicine.展开更多
Intratumoral microbiota has become research hotspots,and emerges as a non-negligent new component of tumor microenvironments(TME),due to its powerful infuence on tumor initiation,metas-tasis,immunosurveillance and pro...Intratumoral microbiota has become research hotspots,and emerges as a non-negligent new component of tumor microenvironments(TME),due to its powerful infuence on tumor initiation,metas-tasis,immunosurveillance and prognosis despite in low-biomass.The accumulations of microbes,and their related components and metabolites within tumor tissues,endow TME with additional pluralistic features which are distinct from the conventional one.Therefore,it's definitely necessary to comprehen-sively delineate the sophisticated landscapes of tumor microbe microenvironment,as well as their func-tions and related underlying mechanisms.Herein,in this review,we focused on the fields of tumor microbe microenvironment,including the heterogeneity of intratumor microbiota in different types of tu-mors,the controversial roles of intratumoral microbiota,the basic features of tumor microbe microenvi-ronment(i.e.,pathogen-associated molecular patterns(PAMPs),typical microbial metabolites,autophagy,infammation,multi-faceted immunomodulation and chemoresistance),as well as the multi-disciplinary approach-based intervention of tumor microbiome for cancer therapy by applying wild-type or engineered live microbes,microbiota metabolites,antibiotics,synthetic biology and rationally de-signed biomaterials.We hope our work will provide valuable insight to deeply understand the interplay of cancer-immune-microbial,and facilitate the development of microbes-based tumor-specific treatments.展开更多
As a typical human pathogenic fungus,Cryptococcus neoformans is a life-threatening invasive fungal pathogen with a worldwide distribution causing w700,000 deaths annually.Cryptococcosis is not just an infection with m...As a typical human pathogenic fungus,Cryptococcus neoformans is a life-threatening invasive fungal pathogen with a worldwide distribution causing w700,000 deaths annually.Cryptococcosis is not just an infection with multi-organ involvement,intracellular survival and extracellular multiplication of the fungus also play important roles in the pathogenesis of C.neoformans infections.Because adequate accumulation of drugs at target organs and cells is still difficult to achieve,an effective delivery strategy is desperately required to treat these infections.Here,we report a bioresponsive micro-to-nano(MTN)system that effectively clears the C.neoformans in vivo.This strategy is based on our in-depth study of the overexpression of matrix metalloproteinase 3(MMP-3)in infectious microenvironments(IMEs)and secreted protein acidic and rich in cysteine(SPARC)in several associated target cells.In this MTN system,bovine serum albumin(BSA,a natural ligand of SPARC)was used for the preparation of nanoparticles(NPs),and then microspheres were constructed by conjugation with a special linker,which mainly consisted of a BSA-binding peptide and an MMP-3-responsive peptide.This MTN system was mechanically captured by the smallest capillaries of the lungs after intravenous injection,and then hydrolyzed into BSA NPs by MMP-3 in the IMEs.The NPs further targeted the lung tissue,brain and infected macrophages based on the overexpression of SPARC,reaching multiple targets and achieving efficient treatment.We have developed a size-tunable strategy where microspheres"shrink"to NPs in IMEs,which effectively combines active and passive targeting and may be especially powerful in the fight against complex fungal infections.展开更多
文摘Objective: Exploring the therapeutic effects of acupuncture for male immune infertility using Meta-analysis. Methods: The literature related to clinical randomized controlled trials (RCTs) on acupuncture for male immune infertility published from the establishment of the database (journal) to 2021 was searched for RR values or OR values and 95% CI as effect indicators. RevMan 5.3 software was applied for meta-analysis. Results: Acupuncture or combination of acupuncture and herbal medicine (hereafter referred to as acupuncture and medicine) or electro-acupuncture, the total effective rate was significantly better than the control group, and the difference was statistically significant [RR = 1.29, 95% CI (1.20, 1.38), p 0.00001];In addition, the efficiency of the combined acupuncture and medicine treatment was better than that of the herbal medicine group alone, and the difference was statistically significant [RR = 1.05, 95%, CI (0.94, 1.16), P = 0.42];The sperm viability in the combined acupuncture and medicine treatment group was significantly better than that in the herbal medicine treatment group alone, and the differences were all statistically significant [MD = 0.04, 95% CI (?0.20, 0.28), P0.74];Sperm forward motion was significantly better in the combination of acupuncture and medicine than in the herbal medicine alone group, and the differences were all statistically significant [MD = 0.66, 95% CI (?0.04, 1.36), P = 0.06];ACP indexes were significantly higher in the combination of acupuncture and medicine than in the herbal medicine alone group, with a statistically significant difference [MD = 20.47, 95% CI (?65.31, 106.25), P = 0.64];The AsAb content in the seminal plasma of either needle medicine or acupuncture was lower than in the homogeneous prednisone group, and the difference was statistically significant [MD = ?7.00, 95% CI (?11.19, ?2.81), P = 0.001];The index of AsAb content in the serum of either needle medicine or acupuncture was lower than that of prednisone group, and the difference was statistically significant [MD = ?5.00, 95% CI (?9.53, ?0.47), P = 0.03]. Conclusion: Based on current evidence, acupuncture is more effective than Western medicine (prednisone) alone in the treatment of male immune infertility, and is more effective when combined with Chinese medicine.
基金This work was supported by grants from National Natural Science Foundation of China(No.82204281)Natural Science Foundation of Chongqing,China(cstc2021jcyj-msxmX0544)+3 种基金Chongqing Science Fund for Distinguished Young Scholars(CSTB2023NSCQ-JQX0021,China)Key project for clinical innovation of Army Medical University(CX2019LC107,China)Innovative Research Group at Higher Educational Institutions in Chongqing(CXQT20006,China)Doctoral Through Train Scientific Research Project of Chongqing(CSTB2022BSXMJCX0004,China).
文摘Intratumoral microbiota has become research hotspots,and emerges as a non-negligent new component of tumor microenvironments(TME),due to its powerful infuence on tumor initiation,metas-tasis,immunosurveillance and prognosis despite in low-biomass.The accumulations of microbes,and their related components and metabolites within tumor tissues,endow TME with additional pluralistic features which are distinct from the conventional one.Therefore,it's definitely necessary to comprehen-sively delineate the sophisticated landscapes of tumor microbe microenvironment,as well as their func-tions and related underlying mechanisms.Herein,in this review,we focused on the fields of tumor microbe microenvironment,including the heterogeneity of intratumor microbiota in different types of tu-mors,the controversial roles of intratumoral microbiota,the basic features of tumor microbe microenvi-ronment(i.e.,pathogen-associated molecular patterns(PAMPs),typical microbial metabolites,autophagy,infammation,multi-faceted immunomodulation and chemoresistance),as well as the multi-disciplinary approach-based intervention of tumor microbiome for cancer therapy by applying wild-type or engineered live microbes,microbiota metabolites,antibiotics,synthetic biology and rationally de-signed biomaterials.We hope our work will provide valuable insight to deeply understand the interplay of cancer-immune-microbial,and facilitate the development of microbes-based tumor-specific treatments.
基金supported by the National Natural Science Foundation of China(No.82073789,and 81673376)the project for Innovative Research Group at Higher Educational Institutions in Chongqing(CXQT20006,China)+1 种基金Chongqing Postgraduate Research and Innovation Project(CYB20106,China)CAS Interdiscipliary Innovation Team(China)。
文摘As a typical human pathogenic fungus,Cryptococcus neoformans is a life-threatening invasive fungal pathogen with a worldwide distribution causing w700,000 deaths annually.Cryptococcosis is not just an infection with multi-organ involvement,intracellular survival and extracellular multiplication of the fungus also play important roles in the pathogenesis of C.neoformans infections.Because adequate accumulation of drugs at target organs and cells is still difficult to achieve,an effective delivery strategy is desperately required to treat these infections.Here,we report a bioresponsive micro-to-nano(MTN)system that effectively clears the C.neoformans in vivo.This strategy is based on our in-depth study of the overexpression of matrix metalloproteinase 3(MMP-3)in infectious microenvironments(IMEs)and secreted protein acidic and rich in cysteine(SPARC)in several associated target cells.In this MTN system,bovine serum albumin(BSA,a natural ligand of SPARC)was used for the preparation of nanoparticles(NPs),and then microspheres were constructed by conjugation with a special linker,which mainly consisted of a BSA-binding peptide and an MMP-3-responsive peptide.This MTN system was mechanically captured by the smallest capillaries of the lungs after intravenous injection,and then hydrolyzed into BSA NPs by MMP-3 in the IMEs.The NPs further targeted the lung tissue,brain and infected macrophages based on the overexpression of SPARC,reaching multiple targets and achieving efficient treatment.We have developed a size-tunable strategy where microspheres"shrink"to NPs in IMEs,which effectively combines active and passive targeting and may be especially powerful in the fight against complex fungal infections.
