Poor seedling emergence is a challenge for direct seeding of rice under deep-sowing field conditions.Here we reveal that UDP-glucosyltransferase OsUGT75A promotes rice seedling emergence under deepsowing conditions by...Poor seedling emergence is a challenge for direct seeding of rice under deep-sowing field conditions.Here we reveal that UDP-glucosyltransferase OsUGT75A promotes rice seedling emergence under deepsowing conditions by increasing shoot length.Expression of OsUGT75A was higher in the middle regions of the shoot and in shoots under deep-sowing conditions.Levels of free abscisic acid(ABA)and jasmonates(JA)were higher in shoots of OsUGT75A mutants than in those of wild-type plants,and OsUGT75A mutants were more sensitive to ABA and JA treatments.Reduced shoot length was attributed to higher ABA INSENSITIVE 3(OsABI3)expression and lower JASMONATE-ZIM domain protein(OsJAZ)expression in shoots.Shoot extension by OsUGT75A is achieved mainly by promotion of cell elongation.An elite haplotype of OsUGT75A associated with increased shoot length was identified among indica rice accessions.OsUGT75A acts to increase seedling emergence under deep-sowing conditions.展开更多
A study was conducted to determine the genetic diversity of 39 determinate and indeterminate tomato inbred lines collected from China,Japan,S. Korea,and USA. Using 35 SSR polymorphic markers,a total of 150 alleles wer...A study was conducted to determine the genetic diversity of 39 determinate and indeterminate tomato inbred lines collected from China,Japan,S. Korea,and USA. Using 35 SSR polymorphic markers,a total of 150 alleles were found with moderate levels of diversity,and a high number of unique alleles existing in these tomato lines. The mean number of alleles per locus was 4.3 and the average poly-morphism information content (PIC) was 0.31. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) clustering at genetic similarity value of 0.85 grouped the inbred lines into four groups,where one USA cultivar formed a separate and more distant cluster. The most similar inbred lines are from USA,both with determinate type,whereas the most different lines are from USA (Us-16) and Japan (Ja-2) with determinate and indeterminate growth habit,respectively. Clustering was consistent with the known information regarding geographical location and growth habit. The genetic distance information reported in this study might be used by breeders when planning future crosses among these inbred lines.展开更多
基金supported by the Key-Area Research and Development Program of Guangdong Province(2022B0202060006)the Natural Science Foundation of Guangdong Province(2023A1515012052,2023A1515012092)+2 种基金the Science and Technology Project of Guangzhou(2023A04J0749,2023A04J1452)the Special Fund for Student Cultivation of Scientific and Technological Innovation of Guangdong Province(pdjh2021b0084)the Double First-Class Discipline Promotion Project of South China Agricultural University(2021B10564001).
文摘Poor seedling emergence is a challenge for direct seeding of rice under deep-sowing field conditions.Here we reveal that UDP-glucosyltransferase OsUGT75A promotes rice seedling emergence under deepsowing conditions by increasing shoot length.Expression of OsUGT75A was higher in the middle regions of the shoot and in shoots under deep-sowing conditions.Levels of free abscisic acid(ABA)and jasmonates(JA)were higher in shoots of OsUGT75A mutants than in those of wild-type plants,and OsUGT75A mutants were more sensitive to ABA and JA treatments.Reduced shoot length was attributed to higher ABA INSENSITIVE 3(OsABI3)expression and lower JASMONATE-ZIM domain protein(OsJAZ)expression in shoots.Shoot extension by OsUGT75A is achieved mainly by promotion of cell elongation.An elite haplotype of OsUGT75A associated with increased shoot length was identified among indica rice accessions.OsUGT75A acts to increase seedling emergence under deep-sowing conditions.
文摘A study was conducted to determine the genetic diversity of 39 determinate and indeterminate tomato inbred lines collected from China,Japan,S. Korea,and USA. Using 35 SSR polymorphic markers,a total of 150 alleles were found with moderate levels of diversity,and a high number of unique alleles existing in these tomato lines. The mean number of alleles per locus was 4.3 and the average poly-morphism information content (PIC) was 0.31. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) clustering at genetic similarity value of 0.85 grouped the inbred lines into four groups,where one USA cultivar formed a separate and more distant cluster. The most similar inbred lines are from USA,both with determinate type,whereas the most different lines are from USA (Us-16) and Japan (Ja-2) with determinate and indeterminate growth habit,respectively. Clustering was consistent with the known information regarding geographical location and growth habit. The genetic distance information reported in this study might be used by breeders when planning future crosses among these inbred lines.
