目的·探究去唾液酸糖蛋白受体1(asialoglycoprotein receptor 1,ASGR1)在肝细胞癌(hepatocellular carcinoma,HCC)中的意义及潜在机制。方法·通过R语言分析癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库中ASGR1在肝癌...目的·探究去唾液酸糖蛋白受体1(asialoglycoprotein receptor 1,ASGR1)在肝细胞癌(hepatocellular carcinoma,HCC)中的意义及潜在机制。方法·通过R语言分析癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库中ASGR1在肝癌患者中的表达情况并绘制相关生存曲线。利用人类蛋白质图谱(The Human Protein Atlas,HPA)数据库获得人体正常肝组织和肝癌组织的免疫组织化学(immunohistochemistry,IHC)数据来分析ASGR1的蛋白表达情况。利用流体动力学尾静脉注射(hydrodynamic tail vein injection,HTVI)递送方法,在免疫完全的小鼠肝脏中敲除Asgr1探究其在体内的致瘤功能,并通过蛋白免疫印迹法(Western blotting,WB)验证基因敲除效率。利用R语言进行京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析及相关性分析,利用基因探针富集(Gene Set Enrichment Analysis,GSEA)软件进行GSEA hallmark相关通路分析,利用实时荧光定量PCR(quantitative real-time PCR,qPCR)在小鼠肝癌组织中验证糖酵解关键基因表达水平。结果·ASGR1在肝癌组织中显著低表达,在肝癌患者中ASGR1的低表达与患者较差的总体生存期(overall survival,OS)、无疾病间隔(disease free interval,DFI)、无进展间隔期(progression free interval,PFI)和疾病特异性生存期(disease specific survival,DSS)相关;肿瘤分级程度越高的肝癌患者ASGR1基因表达水平越低。人体正常肝组织ASGR1蛋白的表达显著高于肝癌组织。在免疫完全的肝细胞癌小鼠模型中,小鼠内源性Asgr1敲除可增加肝组织中肿瘤结节的大小和数量。TCGA数据库中ASGR1低表达组肝癌患者富集到多条癌症及代谢相关通路,ASGR1表达与部分糖酵解关键基因表达呈负相关,Asgr1敲除组的小鼠肝癌组织中糖酵解水平高于对照组,提示ASGR1低表达很可能促进肝癌的生长发展,加强代谢重编程促进肿瘤的合成代谢发展。结论·ASGR1在肝癌患者中表达显著降低,与患者的预后呈正相关;小鼠体内敲除Asgr1可促进肝细胞癌的发生发展;ASGR1可以作为肝癌预后不良的潜在生物标志物和潜在治疗新靶点。展开更多
The EU and China are jointly working for trade facilitation through a new agreement,according to the Delegation of the European Union to China.Thanks to the mutual recognition agreement,which was signed on May 16,the ...The EU and China are jointly working for trade facilitation through a new agreement,according to the Delegation of the European Union to China.Thanks to the mutual recognition agreement,which was signed on May 16,the EU and Chinese trusted traders will enjoy lower costs,simplified procedures and greater predictability in their activities.展开更多
The past three decades have witnessed the formation of a 15-billion lampholder market with industry value as trillions of dollars.China’s lighting industry total output has reached 480 billion yuan in 2013.With the i...The past three decades have witnessed the formation of a 15-billion lampholder market with industry value as trillions of dollars.China’s lighting industry total output has reached 480 billion yuan in 2013.With the increasingly important position of展开更多
Australian business investment is expected to be weak throughout 2015.The Westpac/Melbourne Institute Leading Index predicts the Australian economy is still"sluggish"and will grow at 2.75 percent in 2015.The...Australian business investment is expected to be weak throughout 2015.The Westpac/Melbourne Institute Leading Index predicts the Australian economy is still"sluggish"and will grow at 2.75 percent in 2015.The Index,which indicates the likely pace of economic activity over the next three to nine months,rose by 0.30percentage points in January.Being fiscally challenged,the current Australian Federal and State governments are fiscally展开更多
A new online tool has been unveiled by UK and China to deal with the challenges of climate change.DECC and its Chinese partner,the Energy Research Institute of National Development and Reform Commission,unveiled the&q...A new online tool has been unveiled by UK and China to deal with the challenges of climate change.DECC and its Chinese partner,the Energy Research Institute of National Development and Reform Commission,unveiled the"Global Calculator"at launch events simultaneously held in London and Beijing on January 28.The tool shows that cutting carbon emissions to limit global temperatures to a 2℃rise can be achieved while improving living standards.展开更多
China(Shanghai)Pilot Free Trade Zone(FTZ)will not be the only one of its kind in China.A year after the launching of the Shanghai FTZ,China is planning to set up three new free trade zones to give impetus to its falte...China(Shanghai)Pilot Free Trade Zone(FTZ)will not be the only one of its kind in China.A year after the launching of the Shanghai FTZ,China is planning to set up three new free trade zones to give impetus to its faltering economy.The three FTZs will be established in South China’s Guangdong province,East China’s Fujian province and North China’s Tianjin Municipality from March 1,2015,according to an展开更多
文摘目的·探究去唾液酸糖蛋白受体1(asialoglycoprotein receptor 1,ASGR1)在肝细胞癌(hepatocellular carcinoma,HCC)中的意义及潜在机制。方法·通过R语言分析癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库中ASGR1在肝癌患者中的表达情况并绘制相关生存曲线。利用人类蛋白质图谱(The Human Protein Atlas,HPA)数据库获得人体正常肝组织和肝癌组织的免疫组织化学(immunohistochemistry,IHC)数据来分析ASGR1的蛋白表达情况。利用流体动力学尾静脉注射(hydrodynamic tail vein injection,HTVI)递送方法,在免疫完全的小鼠肝脏中敲除Asgr1探究其在体内的致瘤功能,并通过蛋白免疫印迹法(Western blotting,WB)验证基因敲除效率。利用R语言进行京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析及相关性分析,利用基因探针富集(Gene Set Enrichment Analysis,GSEA)软件进行GSEA hallmark相关通路分析,利用实时荧光定量PCR(quantitative real-time PCR,qPCR)在小鼠肝癌组织中验证糖酵解关键基因表达水平。结果·ASGR1在肝癌组织中显著低表达,在肝癌患者中ASGR1的低表达与患者较差的总体生存期(overall survival,OS)、无疾病间隔(disease free interval,DFI)、无进展间隔期(progression free interval,PFI)和疾病特异性生存期(disease specific survival,DSS)相关;肿瘤分级程度越高的肝癌患者ASGR1基因表达水平越低。人体正常肝组织ASGR1蛋白的表达显著高于肝癌组织。在免疫完全的肝细胞癌小鼠模型中,小鼠内源性Asgr1敲除可增加肝组织中肿瘤结节的大小和数量。TCGA数据库中ASGR1低表达组肝癌患者富集到多条癌症及代谢相关通路,ASGR1表达与部分糖酵解关键基因表达呈负相关,Asgr1敲除组的小鼠肝癌组织中糖酵解水平高于对照组,提示ASGR1低表达很可能促进肝癌的生长发展,加强代谢重编程促进肿瘤的合成代谢发展。结论·ASGR1在肝癌患者中表达显著降低,与患者的预后呈正相关;小鼠体内敲除Asgr1可促进肝细胞癌的发生发展;ASGR1可以作为肝癌预后不良的潜在生物标志物和潜在治疗新靶点。
文摘The EU and China are jointly working for trade facilitation through a new agreement,according to the Delegation of the European Union to China.Thanks to the mutual recognition agreement,which was signed on May 16,the EU and Chinese trusted traders will enjoy lower costs,simplified procedures and greater predictability in their activities.
文摘The past three decades have witnessed the formation of a 15-billion lampholder market with industry value as trillions of dollars.China’s lighting industry total output has reached 480 billion yuan in 2013.With the increasingly important position of
文摘Australian business investment is expected to be weak throughout 2015.The Westpac/Melbourne Institute Leading Index predicts the Australian economy is still"sluggish"and will grow at 2.75 percent in 2015.The Index,which indicates the likely pace of economic activity over the next three to nine months,rose by 0.30percentage points in January.Being fiscally challenged,the current Australian Federal and State governments are fiscally
文摘A new online tool has been unveiled by UK and China to deal with the challenges of climate change.DECC and its Chinese partner,the Energy Research Institute of National Development and Reform Commission,unveiled the"Global Calculator"at launch events simultaneously held in London and Beijing on January 28.The tool shows that cutting carbon emissions to limit global temperatures to a 2℃rise can be achieved while improving living standards.
文摘China(Shanghai)Pilot Free Trade Zone(FTZ)will not be the only one of its kind in China.A year after the launching of the Shanghai FTZ,China is planning to set up three new free trade zones to give impetus to its faltering economy.The three FTZs will be established in South China’s Guangdong province,East China’s Fujian province and North China’s Tianjin Municipality from March 1,2015,according to an
