AIM： To study the effects of hepatic ischemia/ reperfusion （I/R） injury on store-operated calcium channel （SOC） currents （Isoc） in freshly isolated rat Kupffer cells, and the effects of Ca^2＋ channel blockers,...AIM： To study the effects of hepatic ischemia/ reperfusion （I/R） injury on store-operated calcium channel （SOC） currents （Isoc） in freshly isolated rat Kupffer cells, and the effects of Ca^2＋ channel blockers, 2-aminoethoxydiphenyl borate （2-APB）, SK＆F96365, econazole and miconazole, on Isoc in isolated rat Kupffer cells after hepatic I/R injury.METHODS： The model of rat hepatic I/R injury was established. Whole-cell patch-clamp techniques were performed to investigate the effects of 2-APB, SK＆F96365, econazole and miconazole on Isoc in isolated rat Kupffer cells after hepatic I/R injury.RESULTS： I/R injury significantly increased Isoc from -80.4±25.2pA to -159.5±34.5pA （^bp 〈 0.01, n = 30）. 2-APB （20, 40, 60, 80, 100 pmol/L）, SK＆F96365 （5, 10, 20, 40, 50 pmol/L）, econazole （0.1, 0.3, 1, 3, 10 μmol/L） and miconazole （0.1, 0.3, 1, 3, 10 μmol/L） inhibited Isoc in a concentration-dependent manner with IC50 of 37.41 μmol/L （n = 8）, 5.89 μmol/L （n = 11）, 0.21 μmol/L （n = 13）, and 0.28 μmol/L （n = 10）. The peak value of Isoc in the I-V relationship was decreased by the blockers in different concentrations, but the reverse potential of Isoc was not transformed. CONCLUSION： SOC is the main channel for the influx of Ca^2＋ during hepatic I/R injuries. Calcium channel blockers, 2-APB, SK＆F96365, econazole and miconazole,have obviously protective effects on I/R injury, probably by inhibiting Isoc in Kupffer cells and preventing the activation of Kupffer cells.展开更多
BACKGROUND Inflammatory bowel disease(IBD)is rare in patients with glycogen storage disease(GSD).In GSD patients,a decrease in the number of neutrophils leads to prolonged intestinal infection,leading to the formation...BACKGROUND Inflammatory bowel disease(IBD)is rare in patients with glycogen storage disease(GSD).In GSD patients,a decrease in the number of neutrophils leads to prolonged intestinal infection,leading to the formation of chronic inflammation and eventually the development of IBD.Minimally invasive surgery for patients with IBD has been proven to reduce inflammatory responses and postoperative risks and ultimately promote rapid recovery.Herein we discuss minimally invasive surgery and the perioperative management in a patient with GSD and IBD.CASE SUMMARY A 23-year-old male had GSD Ib associated with IBD-like disease for 10 years.Despite standard treatments,such as mesalazine,prednisone and adalimumab,the patient eventually developed colonic stenosis with incomplete ileus.After adequate assessment,the patient was treated with minimally invasive surgery and discharged in stable condition.CONCLUSION Minimally invasive surgery for patients with IBD and GSD is safe,feasible and effective.展开更多
Ball-milled Ti-B-doped sodium aluminum hydride was directly synthesized via mechanical ball-milling of a NaH/Al mixture. The mixture was completely hydrogenated to NaAlH4 after 70 h under hydrogen pressure of 1 MPa. A...Ball-milled Ti-B-doped sodium aluminum hydride was directly synthesized via mechanical ball-milling of a NaH/Al mixture. The mixture was completely hydrogenated to NaAlH4 after 70 h under hydrogen pressure of 1 MPa. And higher hydrogen pressure is beneficial for the conversion from NaH/Al mixture to NaAlH4. The dehydrogenation properties of the as-synthesized Ti-Bdoped sodium aluminum were systematically investigated.The result shows that ball-milled Ti-B has a remarkable catalytic effect on the enhanced dehydrogenation properties of NaAlH4. As-synthesized Ti-B-doped NaAlH_4 sample releases hydrogen at the temperature of about 100 ℃. Approximately 4.15 wt% H2 is released from ballmilled Ti-B-doped NaAlH_4 at 233.7 ℃. Even at 110 ℃, it also releases about 2.83 wt% hydrogen. The apparent activation energy（E_a） for the first step is estimated to be 83.97 k J·mol^-1 using Arrhenius equation. Thus, utilization of ball-milled Ti-B as catalyst would substantially enhance the practical applications of NaAlH_4 for hydrogen storage.展开更多
基金the National Natural Science Foundation of China,No.30270532 Trans-Century Training Programme Foundation for the Talents by the Ministry of Education of China, No. 2002-48Shuguang Program Project of Shanghai Educational Committee,No.02SG20
文摘AIM： To study the effects of hepatic ischemia/ reperfusion （I/R） injury on store-operated calcium channel （SOC） currents （Isoc） in freshly isolated rat Kupffer cells, and the effects of Ca^2＋ channel blockers, 2-aminoethoxydiphenyl borate （2-APB）, SK＆F96365, econazole and miconazole, on Isoc in isolated rat Kupffer cells after hepatic I/R injury.METHODS： The model of rat hepatic I/R injury was established. Whole-cell patch-clamp techniques were performed to investigate the effects of 2-APB, SK＆F96365, econazole and miconazole on Isoc in isolated rat Kupffer cells after hepatic I/R injury.RESULTS： I/R injury significantly increased Isoc from -80.4±25.2pA to -159.5±34.5pA （^bp 〈 0.01, n = 30）. 2-APB （20, 40, 60, 80, 100 pmol/L）, SK＆F96365 （5, 10, 20, 40, 50 pmol/L）, econazole （0.1, 0.3, 1, 3, 10 μmol/L） and miconazole （0.1, 0.3, 1, 3, 10 μmol/L） inhibited Isoc in a concentration-dependent manner with IC50 of 37.41 μmol/L （n = 8）, 5.89 μmol/L （n = 11）, 0.21 μmol/L （n = 13）, and 0.28 μmol/L （n = 10）. The peak value of Isoc in the I-V relationship was decreased by the blockers in different concentrations, but the reverse potential of Isoc was not transformed. CONCLUSION： SOC is the main channel for the influx of Ca^2＋ during hepatic I/R injuries. Calcium channel blockers, 2-APB, SK＆F96365, econazole and miconazole,have obviously protective effects on I/R injury, probably by inhibiting Isoc in Kupffer cells and preventing the activation of Kupffer cells.
文摘BACKGROUND Inflammatory bowel disease(IBD)is rare in patients with glycogen storage disease(GSD).In GSD patients,a decrease in the number of neutrophils leads to prolonged intestinal infection,leading to the formation of chronic inflammation and eventually the development of IBD.Minimally invasive surgery for patients with IBD has been proven to reduce inflammatory responses and postoperative risks and ultimately promote rapid recovery.Herein we discuss minimally invasive surgery and the perioperative management in a patient with GSD and IBD.CASE SUMMARY A 23-year-old male had GSD Ib associated with IBD-like disease for 10 years.Despite standard treatments,such as mesalazine,prednisone and adalimumab,the patient eventually developed colonic stenosis with incomplete ileus.After adequate assessment,the patient was treated with minimally invasive surgery and discharged in stable condition.CONCLUSION Minimally invasive surgery for patients with IBD and GSD is safe,feasible and effective.
基金financially supported by the National Natural Science Foundation of China (Nos. 51501072 and 51471089)the Excellent Young and Middle-aged Scientists of Shandong Province (No.BS2014CL026)+1 种基金the Doctoral Foundation of University of Jinan (No.XBS1448)the Key Laboratory of Advanced Energy Materials Chemistry (No.IRT-13R30)
文摘Ball-milled Ti-B-doped sodium aluminum hydride was directly synthesized via mechanical ball-milling of a NaH/Al mixture. The mixture was completely hydrogenated to NaAlH4 after 70 h under hydrogen pressure of 1 MPa. And higher hydrogen pressure is beneficial for the conversion from NaH/Al mixture to NaAlH4. The dehydrogenation properties of the as-synthesized Ti-Bdoped sodium aluminum were systematically investigated.The result shows that ball-milled Ti-B has a remarkable catalytic effect on the enhanced dehydrogenation properties of NaAlH4. As-synthesized Ti-B-doped NaAlH_4 sample releases hydrogen at the temperature of about 100 ℃. Approximately 4.15 wt% H2 is released from ballmilled Ti-B-doped NaAlH_4 at 233.7 ℃. Even at 110 ℃, it also releases about 2.83 wt% hydrogen. The apparent activation energy（E_a） for the first step is estimated to be 83.97 k J·mol^-1 using Arrhenius equation. Thus, utilization of ball-milled Ti-B as catalyst would substantially enhance the practical applications of NaAlH_4 for hydrogen storage.
