Objective:Discussion on the protectiion of electroacupuncture"Shenmen"on heart and brain injury induced by acute myocardial ischemia in rats from the perspective of the expression of cyclic adenosine monopho...Objective:Discussion on the protectiion of electroacupuncture"Shenmen"on heart and brain injury induced by acute myocardial ischemia in rats from the perspective of the expression of cyclic adenosine monophosphate(cAMP)and cyclic guanosine monophosphate(cGMP).Methods:Thirty male SD rats were randomly divided into normal group,model group,and electroacupuncture group.The electrocardiogram was recorded by the Powerlab 8-lead physiological recording system.The left anterior descending coronary artery was ligated to replicate the rat myocardial ischemia model.The acupuncture group was treated with electroacupuncture on the second day after the model was replicated.After the last electroacupuncture treatment,rat myocardium,hippocampus tissue and abdominal aortic blood were collected,and enzyme-linked immunosorbent assay was used to detect the levels of cAMP and cGMP in myocardium,hippocampus tissue and serum content.Results:Compared with the normal group,the cAMP content in the myocardial tissue of the model group was significantly increased,and the cAMP content in the hippocampus tissue and serum was significantly reduced;compared with the model group,the cAMP content in the myocardial tissue of the electroacupuncture group was decreased,and the hippocampus tissue Compared with the normal group,the content of cGMP in the myocardial tissue and serum of the model group increased,and the content of cGMP in the hippocampus decreased.Compared with the model group,the content of cGMP in the hippocampus of the electroacupuncture group was increased.The cGMP content increased,the serum cGMP content was significantly reduced,and the difference in the cGMP content in myocardial tissue was not statistically significant.Conclusion:Electroacupuncture at"Shenmen"acupoint can significantly improve the expression of cAMP,cGMP and myocardial cAMP in the serum and hippocampus of model rats with acute myocardial ischemia-induced heart and brain injury,but has a lower effect on myocardial cGMP content.展开更多
Objective:To systematically evaluate the efficacy of Moxibustion in the treatment of knee osteoarthritis, and to provide a reasonable supplementary scheme for clinical medical treatment by analyzing the results of Mox...Objective:To systematically evaluate the efficacy of Moxibustion in the treatment of knee osteoarthritis, and to provide a reasonable supplementary scheme for clinical medical treatment by analyzing the results of Moxibustion in the treatment of knee osteoarthritis and related experimental studies.Methods:Computerized retrieval of CNKI, Wanfang, PubMed and other databases from January 2009 to June 2019, published clinical controlled trials of Moxibustion in the treatment of knee osteoarthritis, and retrospective references of the required literature to improve the relevant literature retrieval, if there are documents that can not be downloaded, Manual search was carried out and the literature was included after quality evaluation. Meta-analysis was carried out with Review Manager 5.3 software.Results:A total of 1207 patients with knee osteoarthritis were included in 15pieces of literature. (1) In terms of total effective rate, moxibustion was superior to conventional Western drugs. Meta-analysis showed that RR=1.21,95%CI [1.14,1.28],Z=6.44,P < 0.00001;(2) In VAS score, moxibustion was superior to conventional Western drugs, meta-analysis of two groups: WMD=-2.71, 95% CI [-4.90, -0.52], Z=2.43,P < 0.05;(3) In WOMAC score, moxibustion was superior to conventional Western drugs, meta-analysis of two groups: WMD=-6.79,95%CI [-12.35,-1.23],Z=2.39,P < 0.05;(4) WOMAC pain. In terms of pain score, moxibustion was superior to conventional Western drugs. Meta-analysis showed that WMD=-1.34,95%CI [-2.50,-0.17],Z=2.25,P < 0.05;(5) knee joint function. On the contrary, moxibustion was superior to conventional Western drugs. Meta-analysis showed that WMD = 12.54, 95% CI [6.93, 18.15], Z = 4.28,P < 0.01;(6) In Lysholm index, moxibustion was superior to conventional Western drugs. Meta-analysis showed that WMD = 12.13, 95% CI [6.87, 17.39], Z = 4.52,P < 0.01;(7) In Lequesne index, moxibustion was superior to conventional Western drugs. Routine western drugs, meta-analysis: WMD =-4.22, 95% CI [-5.74, -2.70], Z = 5.45,P < 0.01.Conclusions: The efficacy of Moxibustion in the treatment of knee osteoarthritis is better than that of conventional western drug therapy, but a large sample size and high quality randomized controlled trials are still needed to verify and analyze.展开更多
基金National Nature Science Foundation of China(No.81574083)Key Project of Natural Science Research in Universities of Anhui Province(No.KJ2017A300)。
文摘Objective:Discussion on the protectiion of electroacupuncture"Shenmen"on heart and brain injury induced by acute myocardial ischemia in rats from the perspective of the expression of cyclic adenosine monophosphate(cAMP)and cyclic guanosine monophosphate(cGMP).Methods:Thirty male SD rats were randomly divided into normal group,model group,and electroacupuncture group.The electrocardiogram was recorded by the Powerlab 8-lead physiological recording system.The left anterior descending coronary artery was ligated to replicate the rat myocardial ischemia model.The acupuncture group was treated with electroacupuncture on the second day after the model was replicated.After the last electroacupuncture treatment,rat myocardium,hippocampus tissue and abdominal aortic blood were collected,and enzyme-linked immunosorbent assay was used to detect the levels of cAMP and cGMP in myocardium,hippocampus tissue and serum content.Results:Compared with the normal group,the cAMP content in the myocardial tissue of the model group was significantly increased,and the cAMP content in the hippocampus tissue and serum was significantly reduced;compared with the model group,the cAMP content in the myocardial tissue of the electroacupuncture group was decreased,and the hippocampus tissue Compared with the normal group,the content of cGMP in the myocardial tissue and serum of the model group increased,and the content of cGMP in the hippocampus decreased.Compared with the model group,the content of cGMP in the hippocampus of the electroacupuncture group was increased.The cGMP content increased,the serum cGMP content was significantly reduced,and the difference in the cGMP content in myocardial tissue was not statistically significant.Conclusion:Electroacupuncture at"Shenmen"acupoint can significantly improve the expression of cAMP,cGMP and myocardial cAMP in the serum and hippocampus of model rats with acute myocardial ischemia-induced heart and brain injury,but has a lower effect on myocardial cGMP content.
基金National "973 Plan": Research on moxibustion hot light smoke effect and its biological mechanism(2015CB554504)construction project of scientific research innovation platform of Anhui University: acupuncture theory, technology and application innovation team(2015TD033).
文摘Objective:To systematically evaluate the efficacy of Moxibustion in the treatment of knee osteoarthritis, and to provide a reasonable supplementary scheme for clinical medical treatment by analyzing the results of Moxibustion in the treatment of knee osteoarthritis and related experimental studies.Methods:Computerized retrieval of CNKI, Wanfang, PubMed and other databases from January 2009 to June 2019, published clinical controlled trials of Moxibustion in the treatment of knee osteoarthritis, and retrospective references of the required literature to improve the relevant literature retrieval, if there are documents that can not be downloaded, Manual search was carried out and the literature was included after quality evaluation. Meta-analysis was carried out with Review Manager 5.3 software.Results:A total of 1207 patients with knee osteoarthritis were included in 15pieces of literature. (1) In terms of total effective rate, moxibustion was superior to conventional Western drugs. Meta-analysis showed that RR=1.21,95%CI [1.14,1.28],Z=6.44,P < 0.00001;(2) In VAS score, moxibustion was superior to conventional Western drugs, meta-analysis of two groups: WMD=-2.71, 95% CI [-4.90, -0.52], Z=2.43,P < 0.05;(3) In WOMAC score, moxibustion was superior to conventional Western drugs, meta-analysis of two groups: WMD=-6.79,95%CI [-12.35,-1.23],Z=2.39,P < 0.05;(4) WOMAC pain. In terms of pain score, moxibustion was superior to conventional Western drugs. Meta-analysis showed that WMD=-1.34,95%CI [-2.50,-0.17],Z=2.25,P < 0.05;(5) knee joint function. On the contrary, moxibustion was superior to conventional Western drugs. Meta-analysis showed that WMD = 12.54, 95% CI [6.93, 18.15], Z = 4.28,P < 0.01;(6) In Lysholm index, moxibustion was superior to conventional Western drugs. Meta-analysis showed that WMD = 12.13, 95% CI [6.87, 17.39], Z = 4.52,P < 0.01;(7) In Lequesne index, moxibustion was superior to conventional Western drugs. Routine western drugs, meta-analysis: WMD =-4.22, 95% CI [-5.74, -2.70], Z = 5.45,P < 0.01.Conclusions: The efficacy of Moxibustion in the treatment of knee osteoarthritis is better than that of conventional western drug therapy, but a large sample size and high quality randomized controlled trials are still needed to verify and analyze.
