Objective: To examine whether lipoxin A4 （LXA4） has an inhibitory effect on tumor necrosis factor-α（TNF-α）-induced DNA synthesis of glomerular mesangial cells of rat, and explore the molecular mechanisms of LXA4...Objective: To examine whether lipoxin A4 （LXA4） has an inhibitory effect on tumor necrosis factor-α（TNF-α）-induced DNA synthesis of glomerular mesangial cells of rat, and explore the molecular mechanisms of LXA4 action. Methods: Glomerular mesangial cells of rat were cultured and preincubated with LXA4 at different concentrations, and then treated with TNF-α（10 ng/ml）. DNA synthesis was assessed by the incorporation of [ 3H]-thymidine in mesangial cells. Expression of cyclin E protein was determined by Western blotting analysis. Activities of signal transducers and activators of transcription-3 （STAT3） were analyzed by electrophoretic mobility shift assay （EMSA）. Results: TNF-α-stimulated DNA synthesis of mesangial cells, upregulation of cyclin E protein and STAT3 activities were inhibited by LXA4 in a dose-dependent manner. Conclusion: TNF-α-induced DNA synthesis of mesangial cells can be inhibited by TXA4 probably through the mechanism of Jak1/STAT3 pathway-dependent signal transduction.展开更多
Dear Editor,The activating receptor DNAX accessory molecule-1(DNAM-1)plays an important role in T and natural killer(NK)cell-mediated cytotoxicity via the interaction with its ligands poliovirus receptor(PVR,CD155)and...Dear Editor,The activating receptor DNAX accessory molecule-1(DNAM-1)plays an important role in T and natural killer(NK)cell-mediated cytotoxicity via the interaction with its ligands poliovirus receptor(PVR,CD155)and Nectin-2(CD112).Compared with peripheral blood NK cells,tumor-infiltrating NK cells show reduced expression of DNAM-1 across several solid tumors,including ovarian and breast cancer resulting in impaired NK cell function.Early studies reported an inverse correlation between DNAM-1 expression and its ligands in leukemic blasts and ovarian cancer[1].展开更多
In a recent study published in Nature,Seki et al.reported that cold-induced brown fat activation impedes glycolysis and reduces tumor growth in multiple cancers,1 deepening our understanding of the complex role of fat...In a recent study published in Nature,Seki et al.reported that cold-induced brown fat activation impedes glycolysis and reduces tumor growth in multiple cancers,1 deepening our understanding of the complex role of fat metabolism in cancer.展开更多
文摘Objective: To examine whether lipoxin A4 （LXA4） has an inhibitory effect on tumor necrosis factor-α（TNF-α）-induced DNA synthesis of glomerular mesangial cells of rat, and explore the molecular mechanisms of LXA4 action. Methods: Glomerular mesangial cells of rat were cultured and preincubated with LXA4 at different concentrations, and then treated with TNF-α（10 ng/ml）. DNA synthesis was assessed by the incorporation of [ 3H]-thymidine in mesangial cells. Expression of cyclin E protein was determined by Western blotting analysis. Activities of signal transducers and activators of transcription-3 （STAT3） were analyzed by electrophoretic mobility shift assay （EMSA）. Results: TNF-α-stimulated DNA synthesis of mesangial cells, upregulation of cyclin E protein and STAT3 activities were inhibited by LXA4 in a dose-dependent manner. Conclusion: TNF-α-induced DNA synthesis of mesangial cells can be inhibited by TXA4 probably through the mechanism of Jak1/STAT3 pathway-dependent signal transduction.
基金supported by grants from The Swedish Cancer Society(#CAN 2018/451 and#211524 Pj)The Cancer Research Funds of Radiumhemmet(#161192,#181183,and#211253)+1 种基金The Swedish Society for Medical Research(P17-0134)The Swedish Society of Medicine(SLS-960960).
文摘Dear Editor,The activating receptor DNAX accessory molecule-1(DNAM-1)plays an important role in T and natural killer(NK)cell-mediated cytotoxicity via the interaction with its ligands poliovirus receptor(PVR,CD155)and Nectin-2(CD112).Compared with peripheral blood NK cells,tumor-infiltrating NK cells show reduced expression of DNAM-1 across several solid tumors,including ovarian and breast cancer resulting in impaired NK cell function.Early studies reported an inverse correlation between DNAM-1 expression and its ligands in leukemic blasts and ovarian cancer[1].
基金The authors gratefully acknowledge the support of postdoctoral fellowship form NJCCR(no.COCR23PDF017)to Z.C.and Ludwig Institute for Cancer Research,the Brewster Foundation,and grants from the Breast Cancer Research Foundation,American Cancer Society,and Susan G.Komen Foundation to Y.K.
文摘In a recent study published in Nature,Seki et al.reported that cold-induced brown fat activation impedes glycolysis and reduces tumor growth in multiple cancers,1 deepening our understanding of the complex role of fat metabolism in cancer.
