Purpose:A text generation based multidisciplinary problem identification method is proposed,which does not rely on a large amount of data annotation.Design/methodology/approach:The proposed method first identifies the...Purpose:A text generation based multidisciplinary problem identification method is proposed,which does not rely on a large amount of data annotation.Design/methodology/approach:The proposed method first identifies the research objective types and disciplinary labels of papers using a text classification technique;second,it generates abstractive titles for each paper based on abstract and research objective types using a generative pre-trained language model;third,it extracts problem phrases from generated titles according to regular expression rules;fourth,it creates problem relation networks and identifies the same problems by exploiting a weighted community detection algorithm;finally,it identifies multidisciplinary problems based on the disciplinary labels of papers.Findings:Experiments in the“Carbon Peaking and Carbon Neutrality”field show that the proposed method can effectively identify multidisciplinary research problems.The disciplinary distribution of the identified problems is consistent with our understanding of multidisciplinary collaboration in the field.Research limitations:It is necessary to use the proposed method in other multidisciplinary fields to validate its effectiveness.Practical implications:Multidisciplinary problem identification helps to gather multidisciplinary forces to solve complex real-world problems for the governments,fund valuable multidisciplinary problems for research management authorities,and borrow ideas from other disciplines for researchers.Originality/value:This approach proposes a novel multidisciplinary problem identification method based on text generation,which identifies multidisciplinary problems based on generative abstractive titles of papers without data annotation required by standard sequence labeling techniques.展开更多
Only few glufosinate-tolerant genes,such as phosphinothricin acetyltransferase(PAT)and bialaphos resistance(bar)identified from Streptomyces,are currently available for developing genetically modified rice in agricult...Only few glufosinate-tolerant genes,such as phosphinothricin acetyltransferase(PAT)and bialaphos resistance(bar)identified from Streptomyces,are currently available for developing genetically modified rice in agricultural application.Following the rapid development of genome editing technology,generation of novel glufosinate-tolerant gene resources through artificial evolution of endogenous genes is more promising and highly desirable in rice molecular breeding program.In this study,the endogenous Glutamine synthetase1(OsGS1)was artificially evolved by base-editing-mediated gene evolution(BEMGE)in rice cells to create novel alleles conferring glufosinate tolerance in rice germplasms.Two novel glufosinate-tolerant OsGS1 alleles(OsGS1-AVPS and OsGS1-+AF)and one reported tolerant allele(OsGS1-SGTA)were successfully identified from approximately 4200 independent hygromycin-tolerant calli.Germination assays and spray tests revealed that these three OsGS1 alleles confer glufosinate tolerance in rice.Furthermore,OsGS1-AVPS and OsGS1-SGTA were quickly deployed into the elite rice cultivar Nangeng 46 through precise base editing.Overall,our results demonstrate the feasibility of developing glufosinate-tolerant rice by editing an endogenous rice gene in molecular breeding programs.展开更多
Increasing evidence demonstrates that mammals have different reactions to hypoxia with varied oxygen dynamic patterns.It takes~24 h for tri-gas incubator to achieve steady cell hypoxia,which fails to recapitulate ultr...Increasing evidence demonstrates that mammals have different reactions to hypoxia with varied oxygen dynamic patterns.It takes~24 h for tri-gas incubator to achieve steady cell hypoxia,which fails to recapitulate ultrafast oxygen dynamics of intestinal ischemia/reperfusion(IR)injury.Inspired from the structure of native intestinal villi,we engineered an intestinal organoid chip embedded with engineered artificial microvessels based on coaxial microfluidic technology by using pH-responsive ZIF-8/sodium alginate scaffold.The chip was featured on:(i)eight times the oxygen exchange efficiency compared with the conventional device,tri-gas incubator,(ii)implantation of intestinal organoid reproducing all types of intestinal epithelial cells,and(iii)bio-responsiveness to hypoxia and reoxygenation(HR)by presenting metabolism disorder,inflammatory reaction,and cell apoptosis.Strikingly,it was found for the first time that Olfactomedin 4(Olfm4)was the most significantly downregulated gene under a rapid HR condition by sequencing the RNA from the organoids.Mechanistically,OLFM4 played protective functions on HR-induced cell inflammation and tissue damage by inhibiting the NF-kappa B signaling activation,thus it could be used as a therapeutic target.Altogether,this study overcomes the issue of mismatched oxygen dynamics between in vitro and in vivo,and sets an example of next-generation multisysteminteractive organoid chip for finding precise therapeutic targets of IR injury.展开更多
Herein,we report the synthesis of a biomimic hydrogel adhesive that addresses the poor healing of surgical anastomosis.Dopamine-conjugated xanthan gum(Da-g-Xan)is fabricated using deep insights into the molecular simi...Herein,we report the synthesis of a biomimic hydrogel adhesive that addresses the poor healing of surgical anastomosis.Dopamine-conjugated xanthan gum(Da-g-Xan)is fabricated using deep insights into the molecular similarity between mussels'adhesive and dopamine as well as the structural similarity between barnacle cement proteins and xanthan gum.The hydrogel mimics marine animals’adherence to wet tissue surfaces.Upon applying this adhesive to colonic anastomosis in a rat model,protective effects were shown by significantly improving the bursting pressure.Mechanistically,the architecture of Da-g-Xan hydrogel is maintained by dynamic intermolecular hydrogen bonds that allow the quick release of Da-g-Xan.The free Da-g-Xan can regulate the inflammatory status and induce type 2 macrophage polarization(M2)by specifically interacting with mannose receptors(CD206)revealed by RNA-sequencing and molecular binding assays.Consequently,an appropriate microenvironment for tissue healing is created by the secretion of chemokines and growth factors from M2 macrophages,strengthening the fibroblast migration and proliferation,collagen synthesis and epithelial vascularization.Overall,this study demonstrates an unprecedented strategy for generating an adhesive by synergistic mimicry inspired by two marine animals,and the results show that the Da-g-Xan adhesive augments native tissue regenerative responses,thus enabling enhanced recovery following surgical anastomosis.展开更多
Streptococcus canis Cas9(ScCas9)is an RNAguided endonuclease with NNG protospacer adjacent motif(PAM)specificity whose genomeediting activity in rice is locus-dependent.Here we investigated the performance of a ScCas9...Streptococcus canis Cas9(ScCas9)is an RNAguided endonuclease with NNG protospacer adjacent motif(PAM)specificity whose genomeediting activity in rice is locus-dependent.Here we investigated the performance of a ScCas9 variant named Sc^(++)at different NNG PAM sites in the rice genome;Sc^(++)harbors a T1227K mutation and the substitution of a positively charged loop(residues 367-376).Sc^(++)nuclease achieved broader genome editing compared to the original ScCas9,and its nickase improved targeted base editing in transgenic rice plants.Using the high-efficiency adenine base editor rBE73b,we generated many new OsGS1 alleles suitable for screening of rice germplasm for potential herbicide resistance in the future.The CRISPR/Sc^(++)system expands the genomeediting toolkit for rice.展开更多
Industry integration is a significant trend in modern economic system and industrial development.It has been proved by both theory and practice that choosing the path of integrated development is the inevitable course...Industry integration is a significant trend in modern economic system and industrial development.It has been proved by both theory and practice that choosing the path of integrated development is the inevitable course for expanding the modern industrial system,extending the industry boundary and improving industry competitiveness.The modern service industry and advanced manufacturing industry with the core feature of innovation and development under the leadership of technology need to satisfy the high standard and requirement of development quality,comprehensive competitiveness,demand shift and green development.Under the development trend of“integration of two industries”,profound changes have taken place in the status,industrial form and agglomeration mode of producer services.New models are emerging in the integrated development of advanced manufacturing and modern service industries in the aspects of innovation,input,output,demand and region.To accelerate the in-depth development of“integration of two industries”,under the precondition of respecting the choice of market supply and demand and industrial development trends,the government needs to exert targeted force in supporting key integrated areas,organizing working mechanisms orderly and standardizing market access regulations,and fostering diversified integration development entities and establishing talent systems.展开更多
Tourism is increasingly prominent for economic and social development,both as a strategic pillar industry of national economy and as a modern service industry that satisfies people’s aspirations for a better life.Thi...Tourism is increasingly prominent for economic and social development,both as a strategic pillar industry of national economy and as a modern service industry that satisfies people’s aspirations for a better life.This paper probes into tourism development and opening up from the perspective of the new development pattern with dual circulation,which is of practical implications for the tourism development.Balancedly developing domestic and inbound and outbound tourism-accelerating domestic tourism,expanding inbound tourism,and regulating outbound tourism-is an important way for tourism integrating into the new development pattern.For this point,it is essential to fully play the decisive role of market mechanisms and better play the regulatory role of the government.Multiple measures should be taken for the rapid,high-quality and sustainable development of tourism and for the mutual promotion of domestic and international tourism,so as to lay the foundation for making China to be a world tourism power.展开更多
基金supported by the General Projects of ISTIC Innovation Foundation“Problem innovation solution mining based on text generation model”(MS2024-03).
文摘Purpose:A text generation based multidisciplinary problem identification method is proposed,which does not rely on a large amount of data annotation.Design/methodology/approach:The proposed method first identifies the research objective types and disciplinary labels of papers using a text classification technique;second,it generates abstractive titles for each paper based on abstract and research objective types using a generative pre-trained language model;third,it extracts problem phrases from generated titles according to regular expression rules;fourth,it creates problem relation networks and identifies the same problems by exploiting a weighted community detection algorithm;finally,it identifies multidisciplinary problems based on the disciplinary labels of papers.Findings:Experiments in the“Carbon Peaking and Carbon Neutrality”field show that the proposed method can effectively identify multidisciplinary research problems.The disciplinary distribution of the identified problems is consistent with our understanding of multidisciplinary collaboration in the field.Research limitations:It is necessary to use the proposed method in other multidisciplinary fields to validate its effectiveness.Practical implications:Multidisciplinary problem identification helps to gather multidisciplinary forces to solve complex real-world problems for the governments,fund valuable multidisciplinary problems for research management authorities,and borrow ideas from other disciplines for researchers.Originality/value:This approach proposes a novel multidisciplinary problem identification method based on text generation,which identifies multidisciplinary problems based on generative abstractive titles of papers without data annotation required by standard sequence labeling techniques.
基金supported by grants from the Shenzhen Science and Technology Program(KQTD20180411143628272)Special Funds for Science Technology Innovation and Industrial Development of Shenzhen Dapeng New District(PT202101-02)+3 种基金the Hainan Yazhou Bay Seed Lab(B21HJ0215),the National Natural Science Foundation of China(32102294)the China National Postdoctoral Program for Innovative Talents(BX2020378)the China Postdoctoral Science Foundation(2020M672902)the Central Publicinterest Scientific Institution Basal Research Fund(Y2022PT24).
文摘Only few glufosinate-tolerant genes,such as phosphinothricin acetyltransferase(PAT)and bialaphos resistance(bar)identified from Streptomyces,are currently available for developing genetically modified rice in agricultural application.Following the rapid development of genome editing technology,generation of novel glufosinate-tolerant gene resources through artificial evolution of endogenous genes is more promising and highly desirable in rice molecular breeding program.In this study,the endogenous Glutamine synthetase1(OsGS1)was artificially evolved by base-editing-mediated gene evolution(BEMGE)in rice cells to create novel alleles conferring glufosinate tolerance in rice germplasms.Two novel glufosinate-tolerant OsGS1 alleles(OsGS1-AVPS and OsGS1-+AF)and one reported tolerant allele(OsGS1-SGTA)were successfully identified from approximately 4200 independent hygromycin-tolerant calli.Germination assays and spray tests revealed that these three OsGS1 alleles confer glufosinate tolerance in rice.Furthermore,OsGS1-AVPS and OsGS1-SGTA were quickly deployed into the elite rice cultivar Nangeng 46 through precise base editing.Overall,our results demonstrate the feasibility of developing glufosinate-tolerant rice by editing an endogenous rice gene in molecular breeding programs.
基金the National Natural Science Foundation of China(82270595,82272237,82072223,32171402)the China Postdoctoral Science Foundation(BX20220393,2022M723891)+2 种基金the General Program of Medical Research from the Jiangsu Commission of Health(M2020052)the Jiangsu Key Research and Development Plan(BE2021727)Jiangsu Provincial Medical Innovation Center(CXZX202217).
文摘Increasing evidence demonstrates that mammals have different reactions to hypoxia with varied oxygen dynamic patterns.It takes~24 h for tri-gas incubator to achieve steady cell hypoxia,which fails to recapitulate ultrafast oxygen dynamics of intestinal ischemia/reperfusion(IR)injury.Inspired from the structure of native intestinal villi,we engineered an intestinal organoid chip embedded with engineered artificial microvessels based on coaxial microfluidic technology by using pH-responsive ZIF-8/sodium alginate scaffold.The chip was featured on:(i)eight times the oxygen exchange efficiency compared with the conventional device,tri-gas incubator,(ii)implantation of intestinal organoid reproducing all types of intestinal epithelial cells,and(iii)bio-responsiveness to hypoxia and reoxygenation(HR)by presenting metabolism disorder,inflammatory reaction,and cell apoptosis.Strikingly,it was found for the first time that Olfactomedin 4(Olfm4)was the most significantly downregulated gene under a rapid HR condition by sequencing the RNA from the organoids.Mechanistically,OLFM4 played protective functions on HR-induced cell inflammation and tissue damage by inhibiting the NF-kappa B signaling activation,thus it could be used as a therapeutic target.Altogether,this study overcomes the issue of mismatched oxygen dynamics between in vitro and in vivo,and sets an example of next-generation multisysteminteractive organoid chip for finding precise therapeutic targets of IR injury.
基金funded by the National Major Scientific and Technological Special Project for“Significant New Drugs Development”(2018ZX09J18111-04)General Project of Military Logistics Research(CLB19J025)+1 种基金Innovation Project of Military Medicine(16CXZ007)Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX20_0150).
文摘Herein,we report the synthesis of a biomimic hydrogel adhesive that addresses the poor healing of surgical anastomosis.Dopamine-conjugated xanthan gum(Da-g-Xan)is fabricated using deep insights into the molecular similarity between mussels'adhesive and dopamine as well as the structural similarity between barnacle cement proteins and xanthan gum.The hydrogel mimics marine animals’adherence to wet tissue surfaces.Upon applying this adhesive to colonic anastomosis in a rat model,protective effects were shown by significantly improving the bursting pressure.Mechanistically,the architecture of Da-g-Xan hydrogel is maintained by dynamic intermolecular hydrogen bonds that allow the quick release of Da-g-Xan.The free Da-g-Xan can regulate the inflammatory status and induce type 2 macrophage polarization(M2)by specifically interacting with mannose receptors(CD206)revealed by RNA-sequencing and molecular binding assays.Consequently,an appropriate microenvironment for tissue healing is created by the secretion of chemokines and growth factors from M2 macrophages,strengthening the fibroblast migration and proliferation,collagen synthesis and epithelial vascularization.Overall,this study demonstrates an unprecedented strategy for generating an adhesive by synergistic mimicry inspired by two marine animals,and the results show that the Da-g-Xan adhesive augments native tissue regenerative responses,thus enabling enhanced recovery following surgical anastomosis.
基金supported by grants from the National Natural Science Foundation of China(31871948)the Central Public-interest Scientific Institution Basal Research Fund(Y2020PT26)to H.Z。
文摘Streptococcus canis Cas9(ScCas9)is an RNAguided endonuclease with NNG protospacer adjacent motif(PAM)specificity whose genomeediting activity in rice is locus-dependent.Here we investigated the performance of a ScCas9 variant named Sc^(++)at different NNG PAM sites in the rice genome;Sc^(++)harbors a T1227K mutation and the substitution of a positively charged loop(residues 367-376).Sc^(++)nuclease achieved broader genome editing compared to the original ScCas9,and its nickase improved targeted base editing in transgenic rice plants.Using the high-efficiency adenine base editor rBE73b,we generated many new OsGS1 alleles suitable for screening of rice germplasm for potential herbicide resistance in the future.The CRISPR/Sc^(++)system expands the genomeediting toolkit for rice.
基金supported by the Youth Program of National Natural Science Foundation of China under the theme of“Research on Infl uencing Factors,Mechanism and Stage Optimization of Enterprise Technological Innovation from the Perspective of Transnational Production System”(71903009).
文摘Industry integration is a significant trend in modern economic system and industrial development.It has been proved by both theory and practice that choosing the path of integrated development is the inevitable course for expanding the modern industrial system,extending the industry boundary and improving industry competitiveness.The modern service industry and advanced manufacturing industry with the core feature of innovation and development under the leadership of technology need to satisfy the high standard and requirement of development quality,comprehensive competitiveness,demand shift and green development.Under the development trend of“integration of two industries”,profound changes have taken place in the status,industrial form and agglomeration mode of producer services.New models are emerging in the integrated development of advanced manufacturing and modern service industries in the aspects of innovation,input,output,demand and region.To accelerate the in-depth development of“integration of two industries”,under the precondition of respecting the choice of market supply and demand and industrial development trends,the government needs to exert targeted force in supporting key integrated areas,organizing working mechanisms orderly and standardizing market access regulations,and fostering diversified integration development entities and establishing talent systems.
基金CASS Innovation Project“China’s Service Industry Upgrading Strategy and Major Policy Options in the 14th Five-Year Period”(2020CJY01-B004).
文摘Tourism is increasingly prominent for economic and social development,both as a strategic pillar industry of national economy and as a modern service industry that satisfies people’s aspirations for a better life.This paper probes into tourism development and opening up from the perspective of the new development pattern with dual circulation,which is of practical implications for the tourism development.Balancedly developing domestic and inbound and outbound tourism-accelerating domestic tourism,expanding inbound tourism,and regulating outbound tourism-is an important way for tourism integrating into the new development pattern.For this point,it is essential to fully play the decisive role of market mechanisms and better play the regulatory role of the government.Multiple measures should be taken for the rapid,high-quality and sustainable development of tourism and for the mutual promotion of domestic and international tourism,so as to lay the foundation for making China to be a world tourism power.
