DHA-enriched eggs have gained popularity due to their unique nutritional value,but their flavor can be challenging for some consumers to accept.The study analyzed the correlation of lipids and flavors in DHA-enriched ...DHA-enriched eggs have gained popularity due to their unique nutritional value,but their flavor can be challenging for some consumers to accept.The study analyzed the correlation of lipids and flavors in DHA-enriched egg yolks using comprehensive lipidomics and volatile compound analysis.The results showed that 411 lipids were detected in two egg yolk samples.Among them,148 lipid species,including 48 DHA-containing lipids,were significantly higher in DHA-enriched egg yolks than in Common ones(P<0.05).Furthermore,of the 24 volatile compounds detected,the contents of benzaldehyde,heptanal,hexanal,decanal and 2-nonanone in DHA-enriched egg yolks were significantly higher than in Common egg yolks(P<0.05).The“fishy”smell characteristic of DHAenriched egg yolks was mainly caused by volatile aldehydes,which may be produced through the hydrolysis of lipids in the egg yolk to free fatty acids and further oxidation.Analysis of the correlation network diagram revealed that phospholipids containing docosahexaenoic acid(DHA),linoleic acid,or oleic acid chains were the main contributors to the characteristic flavor of DHA-enriched egg yolks.Overall,this study explored the effect of different lipids on the flavor of DHA-enriched egg yolks and provided a theoretical basis for the production and improvement of DHA-enriched eggs.展开更多
Rapeseed is widely grown in China as an important oil crop. In this study, Shotgun lipidomic methodology based on tandem mass spectrometry but without liquid chromatography was established for simple lipids analysis s...Rapeseed is widely grown in China as an important oil crop. In this study, Shotgun lipidomic methodology based on tandem mass spectrometry but without liquid chromatography was established for simple lipids analysis such as mono-, di-and triacylglycerols(MAGs, DAGs and TAGs) and the derivatives such as free fatty acids(FFAs). Six varieties of rapeseeds belonging to 3 types(B. juncea, B. campestris and B. oleracea) from different areas of China were investigated, with 59 TAG species, 10 DAG species, 5 MAG species and 6 FFA species identified. It was found that erucic acid was mainly present in the form of TAG 62:4(22:1/18:2/22:1), TAG 62:3(22:1/18:1/22:1), and TAG 62:5(22:1/18:3/22:1) in high erucic acid rapeseed oils, while oleic acid was mainly present in the form of TAG 54:2(18:0/18:1/18:1), and TAG 54:4(18:1/18:2/18:1) in high oleic acid rapeseed oils. It was difficult for DAG, MAG and FFA quantification due to their low content with high background content of TAGs. Results showed that the main differences in lipids were not derived from the types of rapeseed, but directly related to varieties.展开更多
As a current analytical instrument for lipidomics profiling with high sensitivity, selectivity, and throughput, mass spectrometry (MS) has been widely used in the field of clinical medicine, nutrition, bromatology, bo...As a current analytical instrument for lipidomics profiling with high sensitivity, selectivity, and throughput, mass spectrometry (MS) has been widely used in the field of clinical medicine, nutrition, bromatology, botany and etc. During the routine use of shotgun- MS and high performance liquid chromatography-MS (HPLC-MS) in lipid analysis, an important problem to be solved is the defective repeatability and stability resulting from signal attenuation or drifts in LC-peak shape and retention time of MS platform. To remove or minimize the influence, extensively accepted and applied QC (quality control) samples must be performed. In this review, we emphasize the importance of the application of QC samples, which could 1) provide the ability to equilibrate the analytical platform at the beginning; 2) act as a quality assurance (QA) procedure during the analytical process; 3) perform signal correction in the data handling with the purpose of reducing analytical variation and improving the precision of quantitative analysis; 4) the data between different laboratories can be compared through the incorporation of particular QC samples-Standard Reference Material (SRM) 1950. In addition, we display 4 types and requirements of QC samples during quality control in lipidomics profiling. Lipidomics profiling based on MS strongly requires commonly accepted guidelines for quality control to perform routine, large-scale researches, so as to integrate data from different scientists for more information.展开更多
The effect of plant (rapeseed and grape seed) polyphenols on lipid metabolism of rat fed with high fat diet was studied. 30 rats were divided into 5 groups including group Z (basal diet), C (high fat diet without plan...The effect of plant (rapeseed and grape seed) polyphenols on lipid metabolism of rat fed with high fat diet was studied. 30 rats were divided into 5 groups including group Z (basal diet), C (high fat diet without plant polyphenol), S (high fat diet with rapeseed polyphenols), R (high fat diet with grape seed polyphenols) and F (combined with rapeseed and grape seed polyphenols). Levels of lipids including TAGs and PLs in rats were investigated by shotgun-electrospray ionization tandem mass spectrometry (shotgun-ESI-MS). Results showed that high fat diet would give rise to the total TAGs and decreased total phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylserine (PS) (p < 0.05) in group C, S, R and F, compared with group Z. Results also showed that supplementary with rapeseed polyphenols or grape seed polyphenols reduced total TAGs and increased total PC, PE and PS (p < 0.05) in group S, R and F, compared with group C. Grape seed polyphenols and rapeseed polyphenols had certain regulatory effect on metabolism diseases such as fatty liver.展开更多
基金supported by the Opening Project of Hubei Key Laboratory of Lipid Chemistry and Nutrition(202106).
文摘DHA-enriched eggs have gained popularity due to their unique nutritional value,but their flavor can be challenging for some consumers to accept.The study analyzed the correlation of lipids and flavors in DHA-enriched egg yolks using comprehensive lipidomics and volatile compound analysis.The results showed that 411 lipids were detected in two egg yolk samples.Among them,148 lipid species,including 48 DHA-containing lipids,were significantly higher in DHA-enriched egg yolks than in Common ones(P<0.05).Furthermore,of the 24 volatile compounds detected,the contents of benzaldehyde,heptanal,hexanal,decanal and 2-nonanone in DHA-enriched egg yolks were significantly higher than in Common egg yolks(P<0.05).The“fishy”smell characteristic of DHAenriched egg yolks was mainly caused by volatile aldehydes,which may be produced through the hydrolysis of lipids in the egg yolk to free fatty acids and further oxidation.Analysis of the correlation network diagram revealed that phospholipids containing docosahexaenoic acid(DHA),linoleic acid,or oleic acid chains were the main contributors to the characteristic flavor of DHA-enriched egg yolks.Overall,this study explored the effect of different lipids on the flavor of DHA-enriched egg yolks and provided a theoretical basis for the production and improvement of DHA-enriched eggs.
基金supported by National Natural Science Foundation of China(Grant No 21105119)Research Foundation of the Director General of Oil Crops Research Institute,Chinese Academy of Agricultural Sciences
文摘Rapeseed is widely grown in China as an important oil crop. In this study, Shotgun lipidomic methodology based on tandem mass spectrometry but without liquid chromatography was established for simple lipids analysis such as mono-, di-and triacylglycerols(MAGs, DAGs and TAGs) and the derivatives such as free fatty acids(FFAs). Six varieties of rapeseeds belonging to 3 types(B. juncea, B. campestris and B. oleracea) from different areas of China were investigated, with 59 TAG species, 10 DAG species, 5 MAG species and 6 FFA species identified. It was found that erucic acid was mainly present in the form of TAG 62:4(22:1/18:2/22:1), TAG 62:3(22:1/18:1/22:1), and TAG 62:5(22:1/18:3/22:1) in high erucic acid rapeseed oils, while oleic acid was mainly present in the form of TAG 54:2(18:0/18:1/18:1), and TAG 54:4(18:1/18:2/18:1) in high oleic acid rapeseed oils. It was difficult for DAG, MAG and FFA quantification due to their low content with high background content of TAGs. Results showed that the main differences in lipids were not derived from the types of rapeseed, but directly related to varieties.
文摘As a current analytical instrument for lipidomics profiling with high sensitivity, selectivity, and throughput, mass spectrometry (MS) has been widely used in the field of clinical medicine, nutrition, bromatology, botany and etc. During the routine use of shotgun- MS and high performance liquid chromatography-MS (HPLC-MS) in lipid analysis, an important problem to be solved is the defective repeatability and stability resulting from signal attenuation or drifts in LC-peak shape and retention time of MS platform. To remove or minimize the influence, extensively accepted and applied QC (quality control) samples must be performed. In this review, we emphasize the importance of the application of QC samples, which could 1) provide the ability to equilibrate the analytical platform at the beginning; 2) act as a quality assurance (QA) procedure during the analytical process; 3) perform signal correction in the data handling with the purpose of reducing analytical variation and improving the precision of quantitative analysis; 4) the data between different laboratories can be compared through the incorporation of particular QC samples-Standard Reference Material (SRM) 1950. In addition, we display 4 types and requirements of QC samples during quality control in lipidomics profiling. Lipidomics profiling based on MS strongly requires commonly accepted guidelines for quality control to perform routine, large-scale researches, so as to integrate data from different scientists for more information.
文摘The effect of plant (rapeseed and grape seed) polyphenols on lipid metabolism of rat fed with high fat diet was studied. 30 rats were divided into 5 groups including group Z (basal diet), C (high fat diet without plant polyphenol), S (high fat diet with rapeseed polyphenols), R (high fat diet with grape seed polyphenols) and F (combined with rapeseed and grape seed polyphenols). Levels of lipids including TAGs and PLs in rats were investigated by shotgun-electrospray ionization tandem mass spectrometry (shotgun-ESI-MS). Results showed that high fat diet would give rise to the total TAGs and decreased total phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylserine (PS) (p < 0.05) in group C, S, R and F, compared with group Z. Results also showed that supplementary with rapeseed polyphenols or grape seed polyphenols reduced total TAGs and increased total PC, PE and PS (p < 0.05) in group S, R and F, compared with group C. Grape seed polyphenols and rapeseed polyphenols had certain regulatory effect on metabolism diseases such as fatty liver.
