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Essential Oil from Carpesium abrotanoides L.Induces Apoptosis via Activating Mitochondrial Pathway in Hepatocellular Carcinoma Cells 被引量:3
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作者 Qiang WANG li-hong pan +6 位作者 Li LIN Ren ZHANG Yu-chao DU Hao CHEN Mi HUANG Kai-wen GUO Xin-zhou YANG 《Current Medical Science》 SCIE CAS 2018年第6期1045-1053,共9页
The effects of essential oil from Carpesium abrotanoides L.(CAEO)on the proliferation and apoptosis of human hepatic cancer cells were investigated in this study.MTT assays indicated that CAEO inhibited the proliferat... The effects of essential oil from Carpesium abrotanoides L.(CAEO)on the proliferation and apoptosis of human hepatic cancer cells were investigated in this study.MTT assays indicated that CAEO inhibited the proliferation of HCC cells with the IC50 values ranging from 41.28±3.06 to 130.36±20.79μg/mL.Moreover,many obviously nuclear morphological changes of apoptotic cells in CAEO-treated HepG2 cells were detected by Hoechst 33258staining and fluorescence microscopy.Flow cytometry was used to detect cell apoptosis and cell cycle,and noticeable findings showed that CAEO arrested cell-cycle at S and G2/M phases.The decreased Bcl-2/Bax protein ratio and the activation of caspase-3,caspase-9 were also detected by Western blotting.All results suggested that CAEO is a potential agent to fight against liver cancer,and the mitochondria-mediated intrinsic apoptotic pathway could be involved in CAEO-mediated apoptosis of human liver carcinoma cells. 展开更多
关键词 Carpesium abrotanoides L ESSENTIAL oil HEPG2 APOPTOSIS MITOCHONDRIAL PATHWAY
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Protective effect of icaritin on focal cerebral ischemic–reperfusion mice 被引量:1
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作者 cheng-hong sun li-hong pan +5 位作者 jian yang jing-chun yao bing-bing li yu-jun tan gui-min zhang ying sun 《Chinese Herbal Medicines》 CAS 2018年第1期40-45,共6页
Objective: To investigate the protective effects of icaritin (ICT), one of the active ingredients in Epimedii Folium, on mouse model of cerebral ischemia-reperfusion (I/R) in vivo. Methods: ICR mice were subject... Objective: To investigate the protective effects of icaritin (ICT), one of the active ingredients in Epimedii Folium, on mouse model of cerebral ischemia-reperfusion (I/R) in vivo. Methods: ICR mice were subjected to an I h transient middle cerebral artery occlusion (MCAO) and fol- lowed by 24 h of reperfusion. Neurological deficits, infarct volume, brain edema and survive rate were measured, respectively. The levels of brain IL-1β, TNF-a, ROS and DNA-binding activity of NF-KB p65 were measured by ELISA kits. The levels of malondialdehyde (MDA) and activities of superoxide dismu- tase (SOD) were detected by spectrophotometry, and the release of nitric oxide (NO) were detected by Griess kit. Results: ICT markedly reduced the neurological deficit scores, brain edema, infarct volume and increased the survival rate of the cerebral I/R mice. The expression of IL-Iβ, TNF-α, NO, MDA and DNA-binding activity of NF-KB p65 were significantly inhibited by ICT, while the activity of SOD were up-regulated at the same time. Conclusion: ICT possessed significant neuroprotective effects in cerebral I/R mice, which might be related to prevent neuroinflammatory and oxidative damage. 展开更多
关键词 brain edema brain infarct ICARITIN focal cerebral ischemic-reperfusion NEUROINFLAMMATION oxidative damage
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