虾青素具有极强的抗氧化、抗癌变和增强免疫力等功能,但由于虾青素水溶解性(分散性)的限制致使其生物利用度和应用范围有很大的局限性。本文以丁二酸酐和合成虾青素为原料(摩尔比为10∶1),以三乙胺为催化剂(与合成虾青素的摩尔比为3∶1)...虾青素具有极强的抗氧化、抗癌变和增强免疫力等功能,但由于虾青素水溶解性(分散性)的限制致使其生物利用度和应用范围有很大的局限性。本文以丁二酸酐和合成虾青素为原料(摩尔比为10∶1),以三乙胺为催化剂(与合成虾青素的摩尔比为3∶1),合成虾青素琥珀酸二酯;经硅胶柱层析,用氯仿-甲醇(90∶10,v/v)混合液进行洗脱纯化;用高效液相色谱对合成的虾青素琥珀酸二酯进行分析,测得其纯度达95%;经紫外光谱扫描,测得其最大吸收波长为487nm,与虾青素的最大吸收波长接近。薄层色谱结果显示,虾青素琥珀酸二酯的极性增大,LC-(APCI)MS/MS分析得到其分子量为796.5,从其裂解碎片中可得到虾青素的碎片离子m/z561.5和m/z579.5。通过1 H NMR和DEPTQ谱进行验证,合成产物为虾青素琥珀酸二酯。展开更多
对石墨炉原子吸收测定 Se 及其价态的条件进行了探讨.为防止 Se 的逸失对不同基体改进剂进行了试验.此外,对环境水样中 Se 及其价态,以及血清和发样中 Se 含量进行了分析.方法的特征含量为1.06×10^(-10)g,水样(n=10)及生物样品(n=4...对石墨炉原子吸收测定 Se 及其价态的条件进行了探讨.为防止 Se 的逸失对不同基体改进剂进行了试验.此外,对环境水样中 Se 及其价态,以及血清和发样中 Se 含量进行了分析.方法的特征含量为1.06×10^(-10)g,水样(n=10)及生物样品(n=4)的相对标准偏差(D_(RS))分别为1.9%及5%,回收率95%～114%.展开更多
It was observed that the atomic fluorescence emission due to signal during the determination of As（Ⅲ） in the mixture of As（Ⅲ） and As（V） could has a 10% to 40% of fluorescence emission As（V）. Besides, interfe...It was observed that the atomic fluorescence emission due to signal during the determination of As（Ⅲ） in the mixture of As（Ⅲ） and As（V） could has a 10% to 40% of fluorescence emission As（V）. Besides, interferes from heavy metals such as Pb（Ⅱ）, Cu（Ⅱ） can cause severe increase of the signals as compared to the insignificant effects caused by Cd（Ⅱ）, Zn（Ⅱ）, Mn（Ⅱ） and Fe（Ⅲ）. On the basis of further studies, the masking agent of 8-hydroxyquinoline was used as an efficient agent to eliminate interference of As（V） emission and the heavy metal of Cu^2＋ and Pb^2＋ in the measurements of arsenic species, After a series standard additions and CRM researches, a sensitive and interference-free analytical procedure was developed for the speciation of arsenic in samples of porewaters and sediments in Poyang Lake, China.展开更多
文摘虾青素具有极强的抗氧化、抗癌变和增强免疫力等功能,但由于虾青素水溶解性(分散性)的限制致使其生物利用度和应用范围有很大的局限性。本文以丁二酸酐和合成虾青素为原料(摩尔比为10∶1),以三乙胺为催化剂(与合成虾青素的摩尔比为3∶1),合成虾青素琥珀酸二酯;经硅胶柱层析,用氯仿-甲醇(90∶10,v/v)混合液进行洗脱纯化;用高效液相色谱对合成的虾青素琥珀酸二酯进行分析,测得其纯度达95%;经紫外光谱扫描,测得其最大吸收波长为487nm,与虾青素的最大吸收波长接近。薄层色谱结果显示,虾青素琥珀酸二酯的极性增大,LC-(APCI)MS/MS分析得到其分子量为796.5,从其裂解碎片中可得到虾青素的碎片离子m/z561.5和m/z579.5。通过1 H NMR和DEPTQ谱进行验证,合成产物为虾青素琥珀酸二酯。
文摘对石墨炉原子吸收测定 Se 及其价态的条件进行了探讨.为防止 Se 的逸失对不同基体改进剂进行了试验.此外,对环境水样中 Se 及其价态,以及血清和发样中 Se 含量进行了分析.方法的特征含量为1.06×10^(-10)g,水样(n=10)及生物样品(n=4)的相对标准偏差(D_(RS))分别为1.9%及5%,回收率95%～114%.
基金The National Natural Science Foundation of China (No. 40103009 40573044) the Hundred-Talent Projects Program by ChineseAcademy of Sciences (No. 0560051057) and the Scientific Foundation of the Returned Overseas Chinese Scholars, State Education Ministry of China(No. 2004527)
文摘It was observed that the atomic fluorescence emission due to signal during the determination of As（Ⅲ） in the mixture of As（Ⅲ） and As（V） could has a 10% to 40% of fluorescence emission As（V）. Besides, interferes from heavy metals such as Pb（Ⅱ）, Cu（Ⅱ） can cause severe increase of the signals as compared to the insignificant effects caused by Cd（Ⅱ）, Zn（Ⅱ）, Mn（Ⅱ） and Fe（Ⅲ）. On the basis of further studies, the masking agent of 8-hydroxyquinoline was used as an efficient agent to eliminate interference of As（V） emission and the heavy metal of Cu^2＋ and Pb^2＋ in the measurements of arsenic species, After a series standard additions and CRM researches, a sensitive and interference-free analytical procedure was developed for the speciation of arsenic in samples of porewaters and sediments in Poyang Lake, China.
