Mammalian olfactory systems have extraordinary ability to sense and identify various trace odorants.Taking advantages of cell culture and micro-fabrication technologies,olfactory cell-or tissue-based biosensor represe...Mammalian olfactory systems have extraordinary ability to sense and identify various trace odorants.Taking advantages of cell culture and micro-fabrication technologies,olfactory cell-or tissue-based biosensor represent a promising platform for in vitro odorant detection.However,in vitro conditions lead to shortened cell/tissue survivals,and the working life of neuron chips is short.The purpose of this study is to develop an in vivo recording and analyzing method for long-term and repeatable detection of odor stimulation.In this study,we implanted penetrating micro-wire array electrode into the olfactory bulb of conscious rats to obtain odor-evoked electrophysiological activities.Then,we investigated the response of ensembles of mitral/tufted cells to stimulation with carvone at a number of concentrations in time and frequency domains.The stable,repeatable odorant responses from up to 16 neural regions could be obtained for at least 3 weeks.Further,we explored the concentration detection sensitivity limitation of developed method,and found the detection low limit of carvone was below10-10mol/L.The result demonstrates that the concentration range of in vivo odorant detection method is much wider than in vitro method.展开更多
In recent years the evolution of olfactory bulb periglomerular cells,as well as the function of periglomerular cells in olfactory encoding,has attracted increasing attention.Studies of neural information encoding base...In recent years the evolution of olfactory bulb periglomerular cells,as well as the function of periglomerular cells in olfactory encoding,has attracted increasing attention.Studies of neural information encoding based on the analysis of simulation and modeling have given rise to electrophysiological models of periglomerular cells,which have an important role in the understanding of the biology of these cells.In this review we provide a brief introduction to the anatomy of the olfactory system and the cell types in the olfactory bulb.We elaborate on the latest progress in the study of the heterogeneity of periglomerular cells based on different classification criteria,such as molecular markers,structure,ion channels and action potentials.Then,we discuss the several existing electrophysiological models of periglomerular cells,and we highlight the problems and defects of these models.Finally,considering our present work,we propose a future direction for electrophysiological investigations of periglomerular cells and for the modeling of periglomerular cells and olfactory information encoding.展开更多
基金supported by the National Key Basic Research Program of China(2009CB320303)the National Natural Science Foundation of China(81027003,60725102)
文摘Mammalian olfactory systems have extraordinary ability to sense and identify various trace odorants.Taking advantages of cell culture and micro-fabrication technologies,olfactory cell-or tissue-based biosensor represent a promising platform for in vitro odorant detection.However,in vitro conditions lead to shortened cell/tissue survivals,and the working life of neuron chips is short.The purpose of this study is to develop an in vivo recording and analyzing method for long-term and repeatable detection of odor stimulation.In this study,we implanted penetrating micro-wire array electrode into the olfactory bulb of conscious rats to obtain odor-evoked electrophysiological activities.Then,we investigated the response of ensembles of mitral/tufted cells to stimulation with carvone at a number of concentrations in time and frequency domains.The stable,repeatable odorant responses from up to 16 neural regions could be obtained for at least 3 weeks.Further,we explored the concentration detection sensitivity limitation of developed method,and found the detection low limit of carvone was below10-10mol/L.The result demonstrates that the concentration range of in vivo odorant detection method is much wider than in vitro method.
基金supported by the National Natural Science Foundation of China (Grant Nos. 30300083,30800248,60875078,and 81171416)the Natural Science Foundation of Zhejiang Province (Grant No.Z1080300)
文摘In recent years the evolution of olfactory bulb periglomerular cells,as well as the function of periglomerular cells in olfactory encoding,has attracted increasing attention.Studies of neural information encoding based on the analysis of simulation and modeling have given rise to electrophysiological models of periglomerular cells,which have an important role in the understanding of the biology of these cells.In this review we provide a brief introduction to the anatomy of the olfactory system and the cell types in the olfactory bulb.We elaborate on the latest progress in the study of the heterogeneity of periglomerular cells based on different classification criteria,such as molecular markers,structure,ion channels and action potentials.Then,we discuss the several existing electrophysiological models of periglomerular cells,and we highlight the problems and defects of these models.Finally,considering our present work,we propose a future direction for electrophysiological investigations of periglomerular cells and for the modeling of periglomerular cells and olfactory information encoding.
