含Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶ADAMTSs(a disintegrin and metallo-proteinase with thrombospondin motifs)是一类新的Zn2+依赖的金属蛋白酶家族,广泛存在于哺乳动物和无脊椎动物体内。从1997年发现第一个ADAMTSs家...含Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶ADAMTSs(a disintegrin and metallo-proteinase with thrombospondin motifs)是一类新的Zn2+依赖的金属蛋白酶家族,广泛存在于哺乳动物和无脊椎动物体内。从1997年发现第一个ADAMTSs家族成员以来,迄今共有19个成员被发现,在保持凝血系统的稳态、器官生成、炎症、生育等方面有重要作用。尽管其中大部分酶的功能尚不清楚,但已有研究显示该家族成员与多种疾病密切相关。ADAMTSs与基质金属蛋白酶MMPs、解聚蛋白样金属蛋白酶ADAMs同属金属蛋白酶家族,但在结构组成、组织细胞分布、底物作用的特异性、酶活性的调节等方面有明显差别。本文综述了其在结构功能及与疾病关系的研究进展。展开更多
Penicillin hydrolysis by penicillin acylase was carried out in reversed micelle with [C14mim]Cl ionic liquids. The experi ment indicated that this catalytic reaction showed better performance in enzymat ic solubility,...Penicillin hydrolysis by penicillin acylase was carried out in reversed micelle with [C14mim]Cl ionic liquids. The experi ment indicated that this catalytic reaction showed better performance in enzymat ic solubility, activity and stability than the reaction in reversed micelle with cetyl trimethyl ammonium bromide （CTAB）. For example, the maximum solubility of penicillin acylase in ionic liquids system was twice more than that in CTAB, an d the maximal enzymatic activity in ionic liquids was 1.3 times than that in CT AB.The outstanding properties of ionic liquids, including low charge density and irregular sharpness of head ring,might be attributable to its distinct head gro up. Ionic liquids made it possible to optimize the enzymatic catalysis in rever sed micelle through altering its interfacial structure effectively.展开更多
文摘含Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶ADAMTSs(a disintegrin and metallo-proteinase with thrombospondin motifs)是一类新的Zn2+依赖的金属蛋白酶家族,广泛存在于哺乳动物和无脊椎动物体内。从1997年发现第一个ADAMTSs家族成员以来,迄今共有19个成员被发现,在保持凝血系统的稳态、器官生成、炎症、生育等方面有重要作用。尽管其中大部分酶的功能尚不清楚,但已有研究显示该家族成员与多种疾病密切相关。ADAMTSs与基质金属蛋白酶MMPs、解聚蛋白样金属蛋白酶ADAMs同属金属蛋白酶家族,但在结构组成、组织细胞分布、底物作用的特异性、酶活性的调节等方面有明显差别。本文综述了其在结构功能及与疾病关系的研究进展。
文摘Penicillin hydrolysis by penicillin acylase was carried out in reversed micelle with [C14mim]Cl ionic liquids. The experi ment indicated that this catalytic reaction showed better performance in enzymat ic solubility, activity and stability than the reaction in reversed micelle with cetyl trimethyl ammonium bromide （CTAB）. For example, the maximum solubility of penicillin acylase in ionic liquids system was twice more than that in CTAB, an d the maximal enzymatic activity in ionic liquids was 1.3 times than that in CT AB.The outstanding properties of ionic liquids, including low charge density and irregular sharpness of head ring,might be attributable to its distinct head gro up. Ionic liquids made it possible to optimize the enzymatic catalysis in rever sed micelle through altering its interfacial structure effectively.
