Objective To investigate the effect of mucin 1(MUC1)on the proliferation and apoptosis of nasopharyngeal carcinoma(NPC)and its regulatory mechanism.Methods The 60 NPC and paired para-cancer normal tissues were collect...Objective To investigate the effect of mucin 1(MUC1)on the proliferation and apoptosis of nasopharyngeal carcinoma(NPC)and its regulatory mechanism.Methods The 60 NPC and paired para-cancer normal tissues were collected from October 2020 to July 2021 in Quanzhou First Hospital.The expression of MUC1 was measured by real-time quantitative PCR(qPCR)in the patients with PNC.The 5-8F and HNE1 cells were transfected with siRNA control(si-control)or siRNA targeting MUC1(si-MUC1).Cell proliferation was analyzed by cell counting kit-8 and colony formation assay,and apoptosis was analyzed by flow cytometry analysis in the 5-8F and HNE1 cells.The qPCR and ELISA were executed to analyze the levels of TNF-αand IL-6.Western blot was performed to measure the expression of MUC1,NFкB and apoptosis-related proteins(Bax and Bcl-2).Results The expression of MUC1 was up-regulated in the NPC tissues,and NPC patients with the high MUC1 expression were inclined to EBV infection,growth and metastasis of NPC.Loss of MUC1 restrained malignant features,including the proliferation and apoptosis,downregulated the expression of p-IкB、p-P65 and Bcl-2 and upregulated the expression of Bax in the NPC cells.Conclusion Downregulation of MUC1 restrained biological characteristics of malignancy,including cell proliferation and apoptosis,by inactivating NF-κB signaling pathway in NPC.展开更多
本文基于荧光共振能量转移(fluorescence resonance energy transfer,FRET)技术设计构建纳米磷光探针。设计构建一种用于活细胞蛋白酶体实时活性检测的探针。主要设计思路为利用纳米金和铱金属配位化合物之间产生的FRET现象,通过目标蛋...本文基于荧光共振能量转移(fluorescence resonance energy transfer,FRET)技术设计构建纳米磷光探针。设计构建一种用于活细胞蛋白酶体实时活性检测的探针。主要设计思路为利用纳米金和铱金属配位化合物之间产生的FRET现象,通过目标蛋白酶的底物多肽连接二者。纳米磷光探针中的上述底物多肽被相应的蛋白酶体特异性识别和切割。FRET供体(铱)配合物与FRET受体(金纳米颗粒)分离,导致FRET现象消失,呈现铱配位配合物的绿色磷光信号。本研究所设计纳米探针针对蛋白酶体(目标蛋白)产生的磷光信号与其酶活性呈正相关,可用于试管和活细胞水平的蛋白酶体活性检测。此外,本研究中所述的酶活检测探针,针对不同的蛋白酶活性检测具有灵活的转变能力,通过多肽序列的替换,可获得目的蛋白酶活性检测磷光探针,大大拓展了其应用。展开更多
文摘Objective To investigate the effect of mucin 1(MUC1)on the proliferation and apoptosis of nasopharyngeal carcinoma(NPC)and its regulatory mechanism.Methods The 60 NPC and paired para-cancer normal tissues were collected from October 2020 to July 2021 in Quanzhou First Hospital.The expression of MUC1 was measured by real-time quantitative PCR(qPCR)in the patients with PNC.The 5-8F and HNE1 cells were transfected with siRNA control(si-control)or siRNA targeting MUC1(si-MUC1).Cell proliferation was analyzed by cell counting kit-8 and colony formation assay,and apoptosis was analyzed by flow cytometry analysis in the 5-8F and HNE1 cells.The qPCR and ELISA were executed to analyze the levels of TNF-αand IL-6.Western blot was performed to measure the expression of MUC1,NFкB and apoptosis-related proteins(Bax and Bcl-2).Results The expression of MUC1 was up-regulated in the NPC tissues,and NPC patients with the high MUC1 expression were inclined to EBV infection,growth and metastasis of NPC.Loss of MUC1 restrained malignant features,including the proliferation and apoptosis,downregulated the expression of p-IкB、p-P65 and Bcl-2 and upregulated the expression of Bax in the NPC cells.Conclusion Downregulation of MUC1 restrained biological characteristics of malignancy,including cell proliferation and apoptosis,by inactivating NF-κB signaling pathway in NPC.
文摘本文基于荧光共振能量转移(fluorescence resonance energy transfer,FRET)技术设计构建纳米磷光探针。设计构建一种用于活细胞蛋白酶体实时活性检测的探针。主要设计思路为利用纳米金和铱金属配位化合物之间产生的FRET现象,通过目标蛋白酶的底物多肽连接二者。纳米磷光探针中的上述底物多肽被相应的蛋白酶体特异性识别和切割。FRET供体(铱)配合物与FRET受体(金纳米颗粒)分离,导致FRET现象消失,呈现铱配位配合物的绿色磷光信号。本研究所设计纳米探针针对蛋白酶体(目标蛋白)产生的磷光信号与其酶活性呈正相关,可用于试管和活细胞水平的蛋白酶体活性检测。此外,本研究中所述的酶活检测探针,针对不同的蛋白酶活性检测具有灵活的转变能力,通过多肽序列的替换,可获得目的蛋白酶活性检测磷光探针,大大拓展了其应用。
