In this study, a rat vascular dementia model was established by permanent bilateral common carotid arterial occlusion. Rats were intraperitoneally injected with puerarin 3 days before modeling, for 45 successive days....In this study, a rat vascular dementia model was established by permanent bilateral common carotid arterial occlusion. Rats were intraperitoneally injected with puerarin 3 days before modeling, for 45 successive days. Results demonstrated that in treated animals hippocampal structures were clear, nerve cells arranged neatly, and cytoplasm was rich in Nissl bodies. The number of cells positive for hypoxia inducible factor-1 alpha, erythropoietin and endothelial nitric oxide synthase was reduced; and the learning and memory abilities of rats were significantly improved. Our experimental findings indicate that puerarin can significantly improve learning and memory in a vascular dementia model, and that the underlying mechanism may be associated with the regulation of the expression of hypoxia inducible factor-1 alpha.展开更多
This study investigated the mechanism underlying electroacupuncture therapy for vascular dementia through electroacupuncture at the acupoints of Baihui (DU20), Dazhui (DU14), and bilateral Shenshu (BL23) in a rat mode...This study investigated the mechanism underlying electroacupuncture therapy for vascular dementia through electroacupuncture at the acupoints of Baihui (DU20), Dazhui (DU14), and bilateral Shenshu (BL23) in a rat model of vascular dementia produced by bilateral middle cerebral artery occlusion. Morris water maze test showed that electroacupuncture improved the learning ability of vascular dementia rats. Western blot assay revealed that the expression of p70 ribosomal protein S6 kinase and ribosomal protein S6 in vascular dementia rats was significantly increased after electroacupuncture, compared with the model group that was not treated with acupuncture. The average escape latency was also shortened after electroacupuncture, and escape strategies in the spatial probe test improved from edge and random searches, to linear and trending swim pathways. The experimental findings indicate that electroacupuncture improves learning and memory ability by up-regulating expression of p70 ribosomal protein S6 kinase and ribosomal protein S6 in the hippocampus of vascular dementia rats.展开更多
OBJECTIVE:To evaluate clinical acupuncture treatment studies for vascular dementia,as well as to collect high-quality evidence related to clinical acupuncture treatment for clinical diagnosis.DATA SOURCES:PubMed datab...OBJECTIVE:To evaluate clinical acupuncture treatment studies for vascular dementia,as well as to collect high-quality evidence related to clinical acupuncture treatment for clinical diagnosis.DATA SOURCES:PubMed database(1966-2010),Embase database(1986-2010),Cochrane Library(Issue 1,2010),Chinese Biomedical Literature Database(1979-2010),China HowNet database(1979-2010),VIP Journals Database(1989-2010),and Wanfang database(1998-2010) were analyzed by computer.DATA SELECTION:Any form of acupuncture(needles,electro-acupuncture,laser acupuncture,pointer,or ear acupuncture),as well as blank,placebo,Western medicine,acupuncture therapy combined with other therapies,and randomized or quasi-randomized controlled studies were included.The priority was given to high-quality randomized,controlled trials.Data were independently extracted from two reviewers and cross-checked in accordance with high to low standards utilizing evidence-based medicine to answer five relevant clinical questions.Statistical outcome indicators were measured using RevMan 5.0.20 software,such as overall function,Hasegawa Dementia Scale score,Functional Activities Questionnaire score,Mini Mental State Examination,and efficacy of daily living activities.MAIN OUTCOME MEASURES:The present study assessed standards of efficacy,including Hasegawa Dementia Scale score,Functional Activities Questionnaire score,Mini Mental State Examination,and efficacy of daily living activities.RESULTS:A total of 11 articles were included,including one with systematic reviews/meta-analysis,one with Cochrane A level,eight with Cochrane B level,and one with Cochrane C level evidence.Acupuncture resulting in improved overall function and cognitive function was superior than Western medicine.A system evaluation showed the following:weighted mean difference(WMD) = 5.64,95% confidence interval(CI):2.87-11.09,P < 0.01;WMD = 6.07,95% CI:3.76-8.38,P < 0.01.Two articles with Class B evidences revealed efficacy at different aspects of acupuncture and moxibustion,which was superior to the conventional body acupuncture group(risk ratio(RR) = 0.03,95% CI:0.01-0.18,P < 0.01;RR = 0.05,95% CI:0.07-0.13,P < 0.05).One A-level evidence study demonstrated that acupuncture improved mental state,daily living activities,and efficacy and effect of cognitive aspects,which was superior to the drug group [cognitive effects(RR = 5.92,95% CI:2.66-13.16,P < 0.01);activities of daily living efficacy(RR = 3.00,95% CI:1.57-5.75,P < 0.01)].The Mini Mental State Examination score resulted in the following:WMD = 3.85,95% CI:1.63-6.07,P < 0.01.One B-grade evidence article revealed that laser irradiation combined with electro-acupuncture was more effective than simple drug treatment(RR = 10.43,95% CI:1.22-89.46,P < 0.05).CONCLUSION:The inclusion of limited clinical studies has verified the efficacy of acupuncture in vascular dementia treatment.Acupuncture or its combination with Chinese herbs provides certain advantages,but most clinical studies have a small sample size.Large sample size,randomized,controlled trials are needed in the future for more definitive results.展开更多
The current study examined a rat model of vascular dementia.The model rats exhibited obvious morphological and ultrastructural changes in neurons in the brain,and significantly reduced dynamin 1 expression in hippocam...The current study examined a rat model of vascular dementia.The model rats exhibited obvious morphological and ultrastructural changes in neurons in the brain,and significantly reduced dynamin 1 expression in hippocampal CA1 region along with decreased learning and memory performance.Following atorvastatin treatment,the morphology and ultrastructure of cells in the model rat brain were significantly improved,dynamin 1 expression in hippocampal CA1 region was significantly enhanced,and learning and memory ability was significantly improved.The results demonstrated that impaired learning and memory abilities in vascular dementia model rats were closely correlated with decreased dynamin 1 expression.These findings indicate that atorvastatin can protect model rats against cognitive impairment by increasing dynamin 1 expression.展开更多
Vascular dementia produced by permanent ligation of bilateral common carotid arteries involves progressive deterioration of intellectual and cognitive function in rats,which are closely associated with the hippocampus...Vascular dementia produced by permanent ligation of bilateral common carotid arteries involves progressive deterioration of intellectual and cognitive function in rats,which are closely associated with the hippocampus.This study used immunohistochemical analysis to detect the expression of glial fibrillary acidic protein and nestin in the hippocampus in a vascular dementia model.The results revealed that both glial fibrillary acidic protein and nestin expression were increased 1 day after permanent ligation of the bilateral common carotid arteries,compared with a sham-operated group.The expression of glial fibrillary acidic protein peaked at 7 days post-surgery.The expression of nestin was a little weaker than that of glial fibrillary acidic protein,and peaked at 14 days(P < 0.01).The expression of both proteins slightly decreased at 21 and 28 days,accompanied by recovery of cerebral blood flow.In conclusion,this study demonstrated that glial fibrillary acidic protein and nestin exhibited dynamic expression in the rat hippocampus after permanent ligation of bilateral common carotid arteries.This finding suggests that dynamic alterations in protein expres-sion play an important role in the pathogenesis of vascular dementia.展开更多
文摘In this study, a rat vascular dementia model was established by permanent bilateral common carotid arterial occlusion. Rats were intraperitoneally injected with puerarin 3 days before modeling, for 45 successive days. Results demonstrated that in treated animals hippocampal structures were clear, nerve cells arranged neatly, and cytoplasm was rich in Nissl bodies. The number of cells positive for hypoxia inducible factor-1 alpha, erythropoietin and endothelial nitric oxide synthase was reduced; and the learning and memory abilities of rats were significantly improved. Our experimental findings indicate that puerarin can significantly improve learning and memory in a vascular dementia model, and that the underlying mechanism may be associated with the regulation of the expression of hypoxia inducible factor-1 alpha.
基金supported by the National Natural Science Foundation of China, No. 81001541
文摘This study investigated the mechanism underlying electroacupuncture therapy for vascular dementia through electroacupuncture at the acupoints of Baihui (DU20), Dazhui (DU14), and bilateral Shenshu (BL23) in a rat model of vascular dementia produced by bilateral middle cerebral artery occlusion. Morris water maze test showed that electroacupuncture improved the learning ability of vascular dementia rats. Western blot assay revealed that the expression of p70 ribosomal protein S6 kinase and ribosomal protein S6 in vascular dementia rats was significantly increased after electroacupuncture, compared with the model group that was not treated with acupuncture. The average escape latency was also shortened after electroacupuncture, and escape strategies in the spatial probe test improved from edge and random searches, to linear and trending swim pathways. The experimental findings indicate that electroacupuncture improves learning and memory ability by up-regulating expression of p70 ribosomal protein S6 kinase and ribosomal protein S6 in the hippocampus of vascular dementia rats.
基金the National Natural Science Foundation of China,No. 30772838the Education Funded Projects of High-Tech Japanese Ministry,No. 343 50537030the Scientific and Technological Support Issue of National "Eleventh Five-Year" Plan Period,No. 2006BAI12B01
文摘OBJECTIVE:To evaluate clinical acupuncture treatment studies for vascular dementia,as well as to collect high-quality evidence related to clinical acupuncture treatment for clinical diagnosis.DATA SOURCES:PubMed database(1966-2010),Embase database(1986-2010),Cochrane Library(Issue 1,2010),Chinese Biomedical Literature Database(1979-2010),China HowNet database(1979-2010),VIP Journals Database(1989-2010),and Wanfang database(1998-2010) were analyzed by computer.DATA SELECTION:Any form of acupuncture(needles,electro-acupuncture,laser acupuncture,pointer,or ear acupuncture),as well as blank,placebo,Western medicine,acupuncture therapy combined with other therapies,and randomized or quasi-randomized controlled studies were included.The priority was given to high-quality randomized,controlled trials.Data were independently extracted from two reviewers and cross-checked in accordance with high to low standards utilizing evidence-based medicine to answer five relevant clinical questions.Statistical outcome indicators were measured using RevMan 5.0.20 software,such as overall function,Hasegawa Dementia Scale score,Functional Activities Questionnaire score,Mini Mental State Examination,and efficacy of daily living activities.MAIN OUTCOME MEASURES:The present study assessed standards of efficacy,including Hasegawa Dementia Scale score,Functional Activities Questionnaire score,Mini Mental State Examination,and efficacy of daily living activities.RESULTS:A total of 11 articles were included,including one with systematic reviews/meta-analysis,one with Cochrane A level,eight with Cochrane B level,and one with Cochrane C level evidence.Acupuncture resulting in improved overall function and cognitive function was superior than Western medicine.A system evaluation showed the following:weighted mean difference(WMD) = 5.64,95% confidence interval(CI):2.87-11.09,P < 0.01;WMD = 6.07,95% CI:3.76-8.38,P < 0.01.Two articles with Class B evidences revealed efficacy at different aspects of acupuncture and moxibustion,which was superior to the conventional body acupuncture group(risk ratio(RR) = 0.03,95% CI:0.01-0.18,P < 0.01;RR = 0.05,95% CI:0.07-0.13,P < 0.05).One A-level evidence study demonstrated that acupuncture improved mental state,daily living activities,and efficacy and effect of cognitive aspects,which was superior to the drug group [cognitive effects(RR = 5.92,95% CI:2.66-13.16,P < 0.01);activities of daily living efficacy(RR = 3.00,95% CI:1.57-5.75,P < 0.01)].The Mini Mental State Examination score resulted in the following:WMD = 3.85,95% CI:1.63-6.07,P < 0.01.One B-grade evidence article revealed that laser irradiation combined with electro-acupuncture was more effective than simple drug treatment(RR = 10.43,95% CI:1.22-89.46,P < 0.05).CONCLUSION:The inclusion of limited clinical studies has verified the efficacy of acupuncture in vascular dementia treatment.Acupuncture or its combination with Chinese herbs provides certain advantages,but most clinical studies have a small sample size.Large sample size,randomized,controlled trials are needed in the future for more definitive results.
基金the Science and Technology Development Program of Hunan Province,No.2008FJ3195
文摘The current study examined a rat model of vascular dementia.The model rats exhibited obvious morphological and ultrastructural changes in neurons in the brain,and significantly reduced dynamin 1 expression in hippocampal CA1 region along with decreased learning and memory performance.Following atorvastatin treatment,the morphology and ultrastructure of cells in the model rat brain were significantly improved,dynamin 1 expression in hippocampal CA1 region was significantly enhanced,and learning and memory ability was significantly improved.The results demonstrated that impaired learning and memory abilities in vascular dementia model rats were closely correlated with decreased dynamin 1 expression.These findings indicate that atorvastatin can protect model rats against cognitive impairment by increasing dynamin 1 expression.
基金the National Natural Science Foundation of China, No. 30973154Chongqing Science and Technology Commission Foundation, No. 2009BB5270Chongqing Municipal Education Commission Foundation, No. KJ090301
文摘Vascular dementia produced by permanent ligation of bilateral common carotid arteries involves progressive deterioration of intellectual and cognitive function in rats,which are closely associated with the hippocampus.This study used immunohistochemical analysis to detect the expression of glial fibrillary acidic protein and nestin in the hippocampus in a vascular dementia model.The results revealed that both glial fibrillary acidic protein and nestin expression were increased 1 day after permanent ligation of the bilateral common carotid arteries,compared with a sham-operated group.The expression of glial fibrillary acidic protein peaked at 7 days post-surgery.The expression of nestin was a little weaker than that of glial fibrillary acidic protein,and peaked at 14 days(P < 0.01).The expression of both proteins slightly decreased at 21 and 28 days,accompanied by recovery of cerebral blood flow.In conclusion,this study demonstrated that glial fibrillary acidic protein and nestin exhibited dynamic expression in the rat hippocampus after permanent ligation of bilateral common carotid arteries.This finding suggests that dynamic alterations in protein expres-sion play an important role in the pathogenesis of vascular dementia.
