对初始孵化到孵化后26d（变态前）的圆斑星鲽（Verasper vazriegatus Temminck et Schlegel）仔鱼的消化系统发生进行形态学和组织学研究。研究表明，在水温为12．5～15．5℃时，初始孵化仔鱼消化道细而直，两端封闭，位于卵黄囊背方。...对初始孵化到孵化后26d（变态前）的圆斑星鲽（Verasper vazriegatus Temminck et Schlegel）仔鱼的消化系统发生进行形态学和组织学研究。研究表明，在水温为12．5～15．5℃时，初始孵化仔鱼消化道细而直，两端封闭，位于卵黄囊背方。随着仔鱼发育，消化道延长，肝胰脏出现。孵化后6d，仔鱼开口，消化道贯通，开始摄食。孵化后10d，消化道明显分为口咽腔、食道、胃、小肠、直肠，黏膜上皮褶皱增多。孵化后14d，卵黄囊消失，仔鱼完全依靠外源性营养。孵化后26d，胃壁较厚处出现胃腺，但不发达，幽门盲囊尚未出现。由结果可见，孵化后6d仔鱼开口，可开始投喂轮虫；孵化后26d，消化系统的结构和功能逐渐完善，消化吸收能力增强，但由于胃的结构和功能尚不完善，此阶段还应以活体饵料为主，一方面可逐渐加大轮虫的投喂量，另一方面可适当投喂较大的卤虫无节幼体等。本研究旨为圆斑星鲽人工育苗中饵料种类及投喂时间的选择提供理论依据。展开更多
Objective To investigate the cell proliferation and differentiation in the developing brain of mouse. Methods C57/BL6 mice were divided into 3 groups at random. Bromodeoxyuridine （BrdU） was injected into the brains ...Objective To investigate the cell proliferation and differentiation in the developing brain of mouse. Methods C57/BL6 mice were divided into 3 groups at random. Bromodeoxyuridine （BrdU） was injected into the brains in different development periods once a day for 7 d. The brains were retrieved 4 weeks after the last BrdU injection. Immunohistochemical and immunofluorescent studies were carried out for detecting cell proliferation （BrdU） and cell differentiation （NeuN, APC, lbal, and S 100β）, respectively. Results The number of BrdU labeled cells decreased significantly with the development of the brain. Cell proliferation was prominent in the cortex and striatum. A small portion of BrdU and NeuN double labeled cells could be detected in the cortex at the early stage of development, and in the striatum and CA of the hippocampus in all groups. The majority of BrdU labeled cells were neuroglia, and the number of neuroglia cells decreased dramatically with brain maturation. Neurogenesis is the major cytogenesis in the dentate gyrus. Conclusion These results demonstrated that cell proliferation, differentiation and survival were age and brain region related.展开更多
文摘对初始孵化到孵化后26d（变态前）的圆斑星鲽（Verasper vazriegatus Temminck et Schlegel）仔鱼的消化系统发生进行形态学和组织学研究。研究表明，在水温为12．5～15．5℃时，初始孵化仔鱼消化道细而直，两端封闭，位于卵黄囊背方。随着仔鱼发育，消化道延长，肝胰脏出现。孵化后6d，仔鱼开口，消化道贯通，开始摄食。孵化后10d，消化道明显分为口咽腔、食道、胃、小肠、直肠，黏膜上皮褶皱增多。孵化后14d，卵黄囊消失，仔鱼完全依靠外源性营养。孵化后26d，胃壁较厚处出现胃腺，但不发达，幽门盲囊尚未出现。由结果可见，孵化后6d仔鱼开口，可开始投喂轮虫；孵化后26d，消化系统的结构和功能逐渐完善，消化吸收能力增强，但由于胃的结构和功能尚不完善，此阶段还应以活体饵料为主，一方面可逐渐加大轮虫的投喂量，另一方面可适当投喂较大的卤虫无节幼体等。本研究旨为圆斑星鲽人工育苗中饵料种类及投喂时间的选择提供理论依据。
基金This work was supported by the grant of National Natural Science Foundation of China （No. 30470598）.
文摘Objective To investigate the cell proliferation and differentiation in the developing brain of mouse. Methods C57/BL6 mice were divided into 3 groups at random. Bromodeoxyuridine （BrdU） was injected into the brains in different development periods once a day for 7 d. The brains were retrieved 4 weeks after the last BrdU injection. Immunohistochemical and immunofluorescent studies were carried out for detecting cell proliferation （BrdU） and cell differentiation （NeuN, APC, lbal, and S 100β）, respectively. Results The number of BrdU labeled cells decreased significantly with the development of the brain. Cell proliferation was prominent in the cortex and striatum. A small portion of BrdU and NeuN double labeled cells could be detected in the cortex at the early stage of development, and in the striatum and CA of the hippocampus in all groups. The majority of BrdU labeled cells were neuroglia, and the number of neuroglia cells decreased dramatically with brain maturation. Neurogenesis is the major cytogenesis in the dentate gyrus. Conclusion These results demonstrated that cell proliferation, differentiation and survival were age and brain region related.
