为探究急性温度胁迫对红耳龟Trachemys scripta elegans消化和免疫功能的影响及饲料添加维生素E(VE)的干预效应。选取健康2龄红耳龟112只,分成未添加VE组和添加VE组(250 mg·kg^(−1)),每组设室温(30℃)、低温(17℃)和高温(35℃),于...为探究急性温度胁迫对红耳龟Trachemys scripta elegans消化和免疫功能的影响及饲料添加维生素E(VE)的干预效应。选取健康2龄红耳龟112只,分成未添加VE组和添加VE组(250 mg·kg^(−1)),每组设室温(30℃)、低温(17℃)和高温(35℃),于胁迫0 h、6 h、12 h取血液和肠道组织,检测血清抗氧化酶和肠道消化酶活性。结果表明,未添加VE组低温下淀粉酶和脂肪酶的活性显著上升,谷胱甘肽(GSH)含量显著降低,高温下胃蛋白酶活性极显著升高,超氧化物歧化酶(SOD)活性显著升高,GSH含量显著下降;添加VE组急性温度胁迫下的SOD活性升幅减小,GSH含量升高,低温下胃蛋白酶活性显著提高,脂肪酶活性显著降低;高温下胃蛋白酶活性极显著降低,淀粉酶活性显著升高。本研究初步阐释了急性温度胁迫对红耳龟的损伤机制,同时发现饲料添加VE具有一定程度的缓解作用,为生产中应对急性温度应激探索了一条绿色可行途径。展开更多
A new keelback snake of the genus Hebius,H.jingdongensis sp.nov.,is reported from Jingdong in Yunnan,China based on a combination of molecular and morphological evidence.Although closest to H.khasiensis and H.boulenge...A new keelback snake of the genus Hebius,H.jingdongensis sp.nov.,is reported from Jingdong in Yunnan,China based on a combination of molecular and morphological evidence.Although closest to H.khasiensis and H.boulengeri phylogenetically,this new species is genetically distinct amongst congeners with the mitochondrial Cyt b uncorrected p-distance ranging from 9.5%(vs.H.octolineatus)to 14.8%(vs.H.andreae).In addition,this new species can be distinguished from its congeners by a combination of the following morphological characters:(1)body cylindrical,tail relatively short,TaL/TL 0.235–0.243;(2)dorsal scale rows 19-19-17;(3)ventrals 163–166,cloacal plate divided,subcaudals 71–74 pairs;(4)supralabials 8,the first two contact with the nasals;(5)infralabials 9 or 10,preocular 1,postoculars 3;(6)body dorsum sandy beige with the sides yellowish-brown decorated with two brick-red stripes on the dorsolateral scale rows in life;(7)sutures between the labials brick-red in life and taupe in preservative;(8)ventral light yellow with the outer part of each scale brick-red in life;(9)ventrolateral stripes present.展开更多
基金supported by the Second Tibetan Plateau Scientific Expedition and Research Program (STEP, 2019QZKK05010503)the China Biodiversity Observation Networks (Sino BON)the support of CIB Herpetological Museum
文摘A new keelback snake of the genus Hebius,H.jingdongensis sp.nov.,is reported from Jingdong in Yunnan,China based on a combination of molecular and morphological evidence.Although closest to H.khasiensis and H.boulengeri phylogenetically,this new species is genetically distinct amongst congeners with the mitochondrial Cyt b uncorrected p-distance ranging from 9.5%(vs.H.octolineatus)to 14.8%(vs.H.andreae).In addition,this new species can be distinguished from its congeners by a combination of the following morphological characters:(1)body cylindrical,tail relatively short,TaL/TL 0.235–0.243;(2)dorsal scale rows 19-19-17;(3)ventrals 163–166,cloacal plate divided,subcaudals 71–74 pairs;(4)supralabials 8,the first two contact with the nasals;(5)infralabials 9 or 10,preocular 1,postoculars 3;(6)body dorsum sandy beige with the sides yellowish-brown decorated with two brick-red stripes on the dorsolateral scale rows in life;(7)sutures between the labials brick-red in life and taupe in preservative;(8)ventral light yellow with the outer part of each scale brick-red in life;(9)ventrolateral stripes present.
