Objective The purpose of this study was to investigate the bacterial communities of biting midges and ticks collected from three sites in the Poyang Lake area,namely,Qunlu Practice Base,Peach Blossom Garden,and Huangt...Objective The purpose of this study was to investigate the bacterial communities of biting midges and ticks collected from three sites in the Poyang Lake area,namely,Qunlu Practice Base,Peach Blossom Garden,and Huangtong Animal Husbandry,and whether vectors carry any bacterial pathogens that may cause diseases to humans,to provide scientific basis for prospective pathogen discovery and disease prevention and control.Methods Using a metataxonomics approach in concert with full-length 16S rRNA gene sequencing and operational phylogenetic unit(OPU)analysis,we characterized the species-level microbial community structure of two important vector species,biting midges and ticks,including 33 arthropod samples comprising 3,885 individuals,collected around Poyang Lake.Results A total of 662 OPUs were classified in biting midges,including 195 known species and 373 potentially new species,and 618 OPUs were classified in ticks,including 217 known species and 326 potentially new species.Surprisingly,OPUs with potentially pathogenicity were detected in both arthropod vectors,with 66 known species of biting midges reported to carry potential pathogens,including Asaia lannensis and Rickettsia bellii,compared to 50 in ticks,such as Acinetobacter lwoffii and Staphylococcus sciuri.We found that Proteobacteria was the most dominant group in both midges and ticks.Furthermore,the outcomes demonstrated that the microbiota of midges and ticks tend to be governed by a few highly abundant bacteria.Pantoea sp7 was predominant in biting midges,while Coxiella sp1 was enriched in ticks.Meanwhile,Coxiella spp.,which may be essential for the survival of Haemaphysalis longicornis Neumann,were detected in all tick samples.The identification of dominant species and pathogens of biting midges and ticks in this study serves to broaden our knowledge associated to microbes of arthropod vectors.Conclusion Biting midges and ticks carry large numbers of known and potentially novel bacteria,and carry a wide range of potentially pathogenic bacteria,which may pose a risk of infection to humans and animals.The microbial communities of midges and ticks tend to be dominated by a few highly abundant bacteria.展开更多
目的助力云南省消除疟疾后防止输入再传播工作。为选择或更换适宜的杀虫剂,控制当地媒介按蚊提供科学依据。方法在中缅边境地区云南省沧源县开展传疟媒介中华按蚊对常用杀虫剂抗药性和抗性基因突变调查,选定沧源县勐董镇勐董社区芒勐寨...目的助力云南省消除疟疾后防止输入再传播工作。为选择或更换适宜的杀虫剂,控制当地媒介按蚊提供科学依据。方法在中缅边境地区云南省沧源县开展传疟媒介中华按蚊对常用杀虫剂抗药性和抗性基因突变调查,选定沧源县勐董镇勐董社区芒勐寨旁永和下7组为监测点,使用吸蚊管于试验前夜在现场采集牛圈内的中华按蚊成蚊,带回实验室饲以10%葡萄糖水供试验用。参照《蚊虫抗药性检测方法生物测定法》(GB/T26347-2010),采用成蚊接触筒法开展中华按蚊对常用杀虫剂的抗药性监测调查。测定结束后分别收集死亡(敏感表型)和存活(抗性表型)个体,用无水乙醇保存带回,提取蚊DNA后,开展抗药性靶标kdr(knockdown resistance)、ace-1基因突变检测。结果接触0.05%高效氯氰菊酯、0.05%高效氯氟氰菊酯、0.05%溴氰菊酯、0.1%残杀威、5%马拉硫磷和1%杀螟硫磷后,中华按蚊首只被击倒的时间分别为5 min 34 s、5 min 11 s、6 min 46 s、4 min 30 s、5 min 13 s和3 min 36 s;击倒率分别为17.49%、17.32%、15.44%、29.72%、57.39%和46.82%。其中,中华按蚊对溴氰菊酯首只击倒时间最长,击倒率最低,表明对溴氰菊酯的抗击倒力最强,其次为高效氯氟氰菊酯和高效氯氰菊酯。中华按蚊对马拉硫磷区分剂量死亡率是98.33%,为敏感群体(S),对杀螟硫磷区分剂量死亡率是90%,为初步抗性群体(M),对高效氯氰菊酯、高效氯氟氰菊酯、溴氰菊酯和残杀威区分剂量死亡率均小于80%,为抗性群体(R)。检测接触拟除虫菊酯类杀虫剂的中华按蚊共386只,kdr基因的1014位点全部为L1014野生型,未检测到突变;检测接触有机磷和氨基甲酸酯的中华按蚊113只,仅存在G119S型突变。中华按蚊ace-1的119位点突变与氨基甲酸酯类杀虫剂抗性相关。结论当地传疟媒介中华按蚊对高效氯氰菊酯等拟除虫菊酯类杀虫剂和残杀威已产生较大抗药性,为防止输入性疟疾引起再传播,今后宜选用马拉硫磷等有机磷类或不同类别杀虫剂联合制剂开展媒介控制工作。展开更多
基金supported by grants from National Key R&D Program of China(2019YFC1200501)Research Units of Discovery of Unknown Bacteria and Function(2018RU010)Capacity Enhancement Project supported by National Institute for Communicable Disease Control and Prevention(China CDC).
文摘Objective The purpose of this study was to investigate the bacterial communities of biting midges and ticks collected from three sites in the Poyang Lake area,namely,Qunlu Practice Base,Peach Blossom Garden,and Huangtong Animal Husbandry,and whether vectors carry any bacterial pathogens that may cause diseases to humans,to provide scientific basis for prospective pathogen discovery and disease prevention and control.Methods Using a metataxonomics approach in concert with full-length 16S rRNA gene sequencing and operational phylogenetic unit(OPU)analysis,we characterized the species-level microbial community structure of two important vector species,biting midges and ticks,including 33 arthropod samples comprising 3,885 individuals,collected around Poyang Lake.Results A total of 662 OPUs were classified in biting midges,including 195 known species and 373 potentially new species,and 618 OPUs were classified in ticks,including 217 known species and 326 potentially new species.Surprisingly,OPUs with potentially pathogenicity were detected in both arthropod vectors,with 66 known species of biting midges reported to carry potential pathogens,including Asaia lannensis and Rickettsia bellii,compared to 50 in ticks,such as Acinetobacter lwoffii and Staphylococcus sciuri.We found that Proteobacteria was the most dominant group in both midges and ticks.Furthermore,the outcomes demonstrated that the microbiota of midges and ticks tend to be governed by a few highly abundant bacteria.Pantoea sp7 was predominant in biting midges,while Coxiella sp1 was enriched in ticks.Meanwhile,Coxiella spp.,which may be essential for the survival of Haemaphysalis longicornis Neumann,were detected in all tick samples.The identification of dominant species and pathogens of biting midges and ticks in this study serves to broaden our knowledge associated to microbes of arthropod vectors.Conclusion Biting midges and ticks carry large numbers of known and potentially novel bacteria,and carry a wide range of potentially pathogenic bacteria,which may pose a risk of infection to humans and animals.The microbial communities of midges and ticks tend to be dominated by a few highly abundant bacteria.
文摘目的助力云南省消除疟疾后防止输入再传播工作。为选择或更换适宜的杀虫剂,控制当地媒介按蚊提供科学依据。方法在中缅边境地区云南省沧源县开展传疟媒介中华按蚊对常用杀虫剂抗药性和抗性基因突变调查,选定沧源县勐董镇勐董社区芒勐寨旁永和下7组为监测点,使用吸蚊管于试验前夜在现场采集牛圈内的中华按蚊成蚊,带回实验室饲以10%葡萄糖水供试验用。参照《蚊虫抗药性检测方法生物测定法》(GB/T26347-2010),采用成蚊接触筒法开展中华按蚊对常用杀虫剂的抗药性监测调查。测定结束后分别收集死亡(敏感表型)和存活(抗性表型)个体,用无水乙醇保存带回,提取蚊DNA后,开展抗药性靶标kdr(knockdown resistance)、ace-1基因突变检测。结果接触0.05%高效氯氰菊酯、0.05%高效氯氟氰菊酯、0.05%溴氰菊酯、0.1%残杀威、5%马拉硫磷和1%杀螟硫磷后,中华按蚊首只被击倒的时间分别为5 min 34 s、5 min 11 s、6 min 46 s、4 min 30 s、5 min 13 s和3 min 36 s;击倒率分别为17.49%、17.32%、15.44%、29.72%、57.39%和46.82%。其中,中华按蚊对溴氰菊酯首只击倒时间最长,击倒率最低,表明对溴氰菊酯的抗击倒力最强,其次为高效氯氟氰菊酯和高效氯氰菊酯。中华按蚊对马拉硫磷区分剂量死亡率是98.33%,为敏感群体(S),对杀螟硫磷区分剂量死亡率是90%,为初步抗性群体(M),对高效氯氰菊酯、高效氯氟氰菊酯、溴氰菊酯和残杀威区分剂量死亡率均小于80%,为抗性群体(R)。检测接触拟除虫菊酯类杀虫剂的中华按蚊共386只,kdr基因的1014位点全部为L1014野生型,未检测到突变;检测接触有机磷和氨基甲酸酯的中华按蚊113只,仅存在G119S型突变。中华按蚊ace-1的119位点突变与氨基甲酸酯类杀虫剂抗性相关。结论当地传疟媒介中华按蚊对高效氯氰菊酯等拟除虫菊酯类杀虫剂和残杀威已产生较大抗药性,为防止输入性疟疾引起再传播,今后宜选用马拉硫磷等有机磷类或不同类别杀虫剂联合制剂开展媒介控制工作。
