[Objectives]To investigate the chemical composition,antioxidant property,and nematicidal activity of Cucumis metuliferus and establish a theoretical foundation for the development of botanical nematicides.[Methods]The...[Objectives]To investigate the chemical composition,antioxidant property,and nematicidal activity of Cucumis metuliferus and establish a theoretical foundation for the development of botanical nematicides.[Methods]The compounds were isolated and purified using silica gel,reversed-phase medium,low-pressure gel Sephadex LH-20 column chromatography,and preparative high-performance liquid chromatography.Their identification was based on physicochemical properties,various spectral analyses,and data reported in the literature.Subsequently,the nematicidal and antioxidant activities of the isolated compounds were evaluated.[Results]A total of four compounds were isolated from C.metuliferus,which were identified as isovitexin-2″-O-glucoside(compound 1),9E,11E-(13-oxo)tridecadienoic acid(compound 2),2E,4E-tridecadienedioic acid(compound 3),and 9E,11E-(13-hydroxy)tridecadienoic acid(compound 4).Compound 1 demonstrated a notable degree of antioxidant activity,achieving a DPPH scavenging rate of 49.40%at a mass concentration of 1 mg/mL.Additionally,compounds 1,3,and 4 exhibited varying levels of nematicidal activity.Specifically,compound 1 displayed strong nematicidal activity at a concentration of 2.0 mg/mL,resulting in a mortality rate of 90.5%against nematodes.Furthermore,compounds 3 and 4 achieved a mortality rate of 100%against nematodes at a concentration of 0.25 mg/mL.[Conclusions]This study reports the first isolation of four compounds:isovitexin-2″-O-glucoside,9E,11E-(13-oxo)tridecadienoic acid,2E,4E-tridecadienedioic acid,and 9E,11E-(13-hydroxy)tridecadienoic acid from C.metuliferus.Notably,2E,4E-tridecadienedioic acid and 9E,11E-(13-oxo)tridecadienoic acid are identified as novel natural products.Among these compounds,isovitexin-2″-O-glucoside,9E,11E-(13-oxo)tridecadienoic acid,and 9E,11E-(13-hydroxy)tridecadienoic acid exhibit nematicidal activity,whereas isovitexin-2″-O-glucoside demonstrates antioxidant properties.展开更多
为研究不同地区的松球壳孢菌株(Sphaeropsis sapinea(Fr.)Pyk et sutton)H6和B2的生物学特性及致病力差异,以形态不同的松球壳孢菌为试验材料,通过致病性测定、生物学特性、生理指标测定、对常见农药敏感性试验进行研究。结果表明:病原...为研究不同地区的松球壳孢菌株(Sphaeropsis sapinea(Fr.)Pyk et sutton)H6和B2的生物学特性及致病力差异,以形态不同的松球壳孢菌为试验材料,通过致病性测定、生物学特性、生理指标测定、对常见农药敏感性试验进行研究。结果表明:病原菌H6和B2侵染樟子松幼苗后,3 d后均开始发病,但菌株B2发病率和致死率显著高于H6;松球壳孢菌H6和B2最适生长条件存在差异,松球壳孢菌H6最适碳源为麦芽糖,最适氮源为蛋白胨,最适光照条件为24 h全光照,最适pH为7,最适培养温度为25℃;菌株B2最适碳源为蔗糖,最适氮源为酵母粉,最适光照条件为24 h全光照,最适pH为8,最适培养温度为30℃。两株病原菌在H_(2)O_(2)胁迫时,除超氧化物歧化酶活性表现平稳外,过氧化物酶活性、过氧化氢酶活性、多酚氧化酶活性、丙二醛活性均呈现先上升后下降的变化趋势,且B2对H_(2)O_(2)反应更为激烈。菌株H6和B2对百菌清敏感性均下降,但菌株H6较菌株B2更为敏感;菌株H6对戊唑醇敏感性下降,菌株B2对戊唑醇较敏感。两株松球壳孢菌在生理特性方面存在差异,最适生长条件不同,并且生长速度存在差异;两株病原菌对农药敏感性不同,且致病力存在差异,经H_(2)O_(2)胁迫处理后,菌株B2酶活反应更为明显。展开更多
基金Supported by Natural Science Foundation Project of Xinjiang Uygur Autonomous Region(2021D01B59)Youth Science and Technology Backbone Project of Xinjiang Academy of Agricultural Sciences(XJNKQ-2021011)+2 种基金General Program of Hainan Natural Science Foundation(319MS109)Major Science and Technology Program of Hainan Province(ZDKJ2021005)Specific Research Fund of the Innovation Platform for Academicians of Hainan Province(YSPTZX202141).
文摘[Objectives]To investigate the chemical composition,antioxidant property,and nematicidal activity of Cucumis metuliferus and establish a theoretical foundation for the development of botanical nematicides.[Methods]The compounds were isolated and purified using silica gel,reversed-phase medium,low-pressure gel Sephadex LH-20 column chromatography,and preparative high-performance liquid chromatography.Their identification was based on physicochemical properties,various spectral analyses,and data reported in the literature.Subsequently,the nematicidal and antioxidant activities of the isolated compounds were evaluated.[Results]A total of four compounds were isolated from C.metuliferus,which were identified as isovitexin-2″-O-glucoside(compound 1),9E,11E-(13-oxo)tridecadienoic acid(compound 2),2E,4E-tridecadienedioic acid(compound 3),and 9E,11E-(13-hydroxy)tridecadienoic acid(compound 4).Compound 1 demonstrated a notable degree of antioxidant activity,achieving a DPPH scavenging rate of 49.40%at a mass concentration of 1 mg/mL.Additionally,compounds 1,3,and 4 exhibited varying levels of nematicidal activity.Specifically,compound 1 displayed strong nematicidal activity at a concentration of 2.0 mg/mL,resulting in a mortality rate of 90.5%against nematodes.Furthermore,compounds 3 and 4 achieved a mortality rate of 100%against nematodes at a concentration of 0.25 mg/mL.[Conclusions]This study reports the first isolation of four compounds:isovitexin-2″-O-glucoside,9E,11E-(13-oxo)tridecadienoic acid,2E,4E-tridecadienedioic acid,and 9E,11E-(13-hydroxy)tridecadienoic acid from C.metuliferus.Notably,2E,4E-tridecadienedioic acid and 9E,11E-(13-oxo)tridecadienoic acid are identified as novel natural products.Among these compounds,isovitexin-2″-O-glucoside,9E,11E-(13-oxo)tridecadienoic acid,and 9E,11E-(13-hydroxy)tridecadienoic acid exhibit nematicidal activity,whereas isovitexin-2″-O-glucoside demonstrates antioxidant properties.
