Pigeons and certain other avian species produce a milklike secretion in their crop sacs to nourish offspring,yet the detailed processes involved are not fully elucidated.This study investigated the crop sacs of 225-da...Pigeons and certain other avian species produce a milklike secretion in their crop sacs to nourish offspring,yet the detailed processes involved are not fully elucidated.This study investigated the crop sacs of 225-day-old unpaired non-lactating male pigeons(MN)and males initiating lactation on the first day after incubation(ML).Using RNA sequencing,ribosomeprofiling,andsingle-cell transcriptome sequencing(scRNA-seq),we identified a significant up-regulation of genes associated with ribosome assembly and protein synthesis in ML compared to MN.Results from scRNA-seq analysis identified 12distinct cell types and 22 clusters,with secretory epithelial cells(SECs)exhibiting marked expression of plasma cell markers,including IGLL1 and MZB1.RNA fluorescence in situ hybridization(RNA FISH)and IgY quantification confirmed the critical role of SECs in producing endogenous IgY during lactation.We propose that fibroblast-derived BAFF signals activate SECs,mimicking B cell transformation and enhancing protein production through the unfolded protein response(UPR).These findings shed light on the cellular dynamics of pigeon milk production and contribute to a broader understanding of avian biology.展开更多
试验旨在探究“2+2”生产模式下不同粗蛋白质水平对白王种鸽繁殖性能和乳鸽生长性能的影响。试验选取产蛋性能相近的300日龄健康种鸽168对,随机分为4组,每组7个重复,每个重复6对种鸽,每对种鸽哺育2只乳鸽。试验Ⅰ组、Ⅱ组、Ⅲ组和Ⅳ组...试验旨在探究“2+2”生产模式下不同粗蛋白质水平对白王种鸽繁殖性能和乳鸽生长性能的影响。试验选取产蛋性能相近的300日龄健康种鸽168对,随机分为4组,每组7个重复,每个重复6对种鸽,每对种鸽哺育2只乳鸽。试验Ⅰ组、Ⅱ组、Ⅲ组和Ⅳ组分别饲喂粗蛋白质水平为13.43%、15.04%、16.64%和18.25%的饲粮。试验期46 d,其中孵化期18 d (1~18 d),哺乳期28 d (19~46 d)。结果显示,试验Ⅱ组、Ⅲ组产蛋间隔显著低于试验Ⅰ组(P<0.05),试验Ⅲ组种蛋受精率显著高于试验Ⅰ组、Ⅳ组(P<0.05),试验Ⅲ组入孵蛋孵化率极显著高于试验Ⅰ组、Ⅱ组、Ⅳ组(P<0.01)。试验Ⅲ组乳鸽28日龄体重显著高于试验Ⅰ组(P<0.05),乳鸽屠宰率极显著高于试验Ⅰ组(P<0.01),乳鸽胸肌率显著高于试验Ⅰ组、Ⅱ组、Ⅳ组(P<0.05)。试验Ⅱ组、Ⅲ组、Ⅳ组窝料重比1极显著低于试验Ⅰ组(P<0.01)。研究表明,不同粗蛋白质水平影响了种鸽的繁殖性能、乳鸽的生长性能以及窝料重比,在“2+2”生产模式下,建议白王种鸽饲粮适宜粗蛋白质水平为16.64%。展开更多
基金supported by the Department of Agriculture and Rural Affairs of Jiangxi Province,China (JXARS-09)Science and Technology Program of Guangdong Province,China (2020B1212060060)。
文摘Pigeons and certain other avian species produce a milklike secretion in their crop sacs to nourish offspring,yet the detailed processes involved are not fully elucidated.This study investigated the crop sacs of 225-day-old unpaired non-lactating male pigeons(MN)and males initiating lactation on the first day after incubation(ML).Using RNA sequencing,ribosomeprofiling,andsingle-cell transcriptome sequencing(scRNA-seq),we identified a significant up-regulation of genes associated with ribosome assembly and protein synthesis in ML compared to MN.Results from scRNA-seq analysis identified 12distinct cell types and 22 clusters,with secretory epithelial cells(SECs)exhibiting marked expression of plasma cell markers,including IGLL1 and MZB1.RNA fluorescence in situ hybridization(RNA FISH)and IgY quantification confirmed the critical role of SECs in producing endogenous IgY during lactation.We propose that fibroblast-derived BAFF signals activate SECs,mimicking B cell transformation and enhancing protein production through the unfolded protein response(UPR).These findings shed light on the cellular dynamics of pigeon milk production and contribute to a broader understanding of avian biology.
文摘试验旨在探究“2+2”生产模式下不同粗蛋白质水平对白王种鸽繁殖性能和乳鸽生长性能的影响。试验选取产蛋性能相近的300日龄健康种鸽168对,随机分为4组,每组7个重复,每个重复6对种鸽,每对种鸽哺育2只乳鸽。试验Ⅰ组、Ⅱ组、Ⅲ组和Ⅳ组分别饲喂粗蛋白质水平为13.43%、15.04%、16.64%和18.25%的饲粮。试验期46 d,其中孵化期18 d (1~18 d),哺乳期28 d (19~46 d)。结果显示,试验Ⅱ组、Ⅲ组产蛋间隔显著低于试验Ⅰ组(P<0.05),试验Ⅲ组种蛋受精率显著高于试验Ⅰ组、Ⅳ组(P<0.05),试验Ⅲ组入孵蛋孵化率极显著高于试验Ⅰ组、Ⅱ组、Ⅳ组(P<0.01)。试验Ⅲ组乳鸽28日龄体重显著高于试验Ⅰ组(P<0.05),乳鸽屠宰率极显著高于试验Ⅰ组(P<0.01),乳鸽胸肌率显著高于试验Ⅰ组、Ⅱ组、Ⅳ组(P<0.05)。试验Ⅱ组、Ⅲ组、Ⅳ组窝料重比1极显著低于试验Ⅰ组(P<0.01)。研究表明,不同粗蛋白质水平影响了种鸽的繁殖性能、乳鸽的生长性能以及窝料重比,在“2+2”生产模式下,建议白王种鸽饲粮适宜粗蛋白质水平为16.64%。
