Point-of-care testing(POCT)is the practice of diagnosing and monitoring diseases where the patient is located,as opposed to traditional treatment conducted solely in a medical laboratory or other clinical setting.POCT...Point-of-care testing(POCT)is the practice of diagnosing and monitoring diseases where the patient is located,as opposed to traditional treatment conducted solely in a medical laboratory or other clinical setting.POCT has been less common in the recent past due to a lack of portable medical devices capable of facilitating effective medical testing.However,recent growth has occurred in this field due to advances in diagnostic technologies,device miniaturization,and progress in wearable electronics.Among these developments,electrochemical sensors have attracted interest in the POCT field due to their high sensitivity,compact size,and affordability.They are used in various applications,from disease diagnosis to health status monitoring.In this paper we explore recent advancements in electrochemical sensors,the methods of fabricating them,and the various types of sensing mechanisms that can be used.Furthermore,we delve into methods for immobilizing specific biorecognition elements,including enzymes,antibodies,and aptamers,onto electrode surfaces and how these sensors are used in real-world POCT settings.展开更多
该文构建一种基于靶标诱导滚环扩增(rolling circle amplification,RCA)的无标记适配体快速检测赭曲霉毒素A(ochratoxin A,OTA)生物传感器。该生物传感器探针由RCA引物与OTA适配体两部分组成,在OTA存在的环境中,OTA适配体特异性识别靶标...该文构建一种基于靶标诱导滚环扩增(rolling circle amplification,RCA)的无标记适配体快速检测赭曲霉毒素A(ochratoxin A,OTA)生物传感器。该生物传感器探针由RCA引物与OTA适配体两部分组成,在OTA存在的环境中,OTA适配体特异性识别靶标,探针结构被打开,RCA引物与环状DNA模板(circular DNA template,CT)结合开启RCA反应,加入核酸染料SYBR Gold产生荧光信号。此生物传感器具有较高的特异性,检测限为6.6×10^(-2)nmol/L,线性检测范围为6.6×10^(-2)~660 nmol/L,可用于具体的分析检测。此生物传感器无需复杂化学修饰且操作简单,在食品安全检测中具有良好的应用前景。展开更多
基金supported by the National Research Foundation of Korea(No.2021R1A2B5B03001691).
文摘Point-of-care testing(POCT)is the practice of diagnosing and monitoring diseases where the patient is located,as opposed to traditional treatment conducted solely in a medical laboratory or other clinical setting.POCT has been less common in the recent past due to a lack of portable medical devices capable of facilitating effective medical testing.However,recent growth has occurred in this field due to advances in diagnostic technologies,device miniaturization,and progress in wearable electronics.Among these developments,electrochemical sensors have attracted interest in the POCT field due to their high sensitivity,compact size,and affordability.They are used in various applications,from disease diagnosis to health status monitoring.In this paper we explore recent advancements in electrochemical sensors,the methods of fabricating them,and the various types of sensing mechanisms that can be used.Furthermore,we delve into methods for immobilizing specific biorecognition elements,including enzymes,antibodies,and aptamers,onto electrode surfaces and how these sensors are used in real-world POCT settings.
文摘该文构建一种基于靶标诱导滚环扩增(rolling circle amplification,RCA)的无标记适配体快速检测赭曲霉毒素A(ochratoxin A,OTA)生物传感器。该生物传感器探针由RCA引物与OTA适配体两部分组成,在OTA存在的环境中,OTA适配体特异性识别靶标,探针结构被打开,RCA引物与环状DNA模板(circular DNA template,CT)结合开启RCA反应,加入核酸染料SYBR Gold产生荧光信号。此生物传感器具有较高的特异性,检测限为6.6×10^(-2)nmol/L,线性检测范围为6.6×10^(-2)~660 nmol/L,可用于具体的分析检测。此生物传感器无需复杂化学修饰且操作简单,在食品安全检测中具有良好的应用前景。
