Deltamethrin(DEL),a commonly used pyrethroid pesticide,results in higher reactive oxygen species(ROS)levels in aquatic animals,which consequently unbalance the redox state.Phlorizin(PHL)is a flavonoid and a natural pr...Deltamethrin(DEL),a commonly used pyrethroid pesticide,results in higher reactive oxygen species(ROS)levels in aquatic animals,which consequently unbalance the redox state.Phlorizin(PHL)is a flavonoid and a natural product promising to prevent or reduce pesticide-induced oxidative stress.Artemia is a micro-crustacean widely used in marine hatcheries and an experimental aquatic organism for environmental toxicology research.This research aimed to evaluate the toxicity of DEL on Artemia and the antioxidative effect of PHL against the toxicity.Results show that 0.08-mg/mL PHL exerted its antioxidative effects on hatching percentage of the cysts in 24-h incubation and on body length and survival rate of Artemia in 12-d culture.After 12-d culture,12-,24-,and 36-h DEL exposure showed significant drops in SOD,CAT,and GSH-Px enzyme activities,and significant increases in ROS and malondialdehyde(MDA)levels in Artemia(P<0.05).On the contrary,0.08-mg/mL PHL application improved the enzyme activities and decreased the ROS and MDA levels(P<0.05).Moreover,0.08-mg/mL PHL significantly increased mRNA expression levels of Cu/Zn SOD,CAT,GST,HO-1,NQO1,and Nrf2,and decreased mRNA expression level of Keap1 in the DEL-exposed Artemia(P<0.05).Therefore,DEL is toxic to Artemia,while PHL alleviates DEL-induced oxidative damage by possibly regulating the Nrf2signaling pathway.This study provided a theoretical basis for PHL to reduce pesticide-induced toxicity in aquatic animals.展开更多
萘普生的广泛使用导致其在环境中持续输入与积累,对生态环境造成潜在的危害。金属离子Cu^(2+)虽然在自然界中普遍存在,但是萘普生和Cu^(2+)的联合毒性效应目前尚不清楚。开展了毒性预实验,根据Cu^(2+)和萘普生96 h LC 50值(毒性单位比4...萘普生的广泛使用导致其在环境中持续输入与积累,对生态环境造成潜在的危害。金属离子Cu^(2+)虽然在自然界中普遍存在,但是萘普生和Cu^(2+)的联合毒性效应目前尚不清楚。开展了毒性预实验,根据Cu^(2+)和萘普生96 h LC 50值(毒性单位比4∶1,2∶1,1∶1,1∶2,1∶4)对斑马鱼胚胎进行混合暴露,采用相加指数(AI)法评价联合毒性影响。实验结果表明:萘普生和Cu^(2+)单独暴露对斑马鱼胚胎产生发育毒性,萘普生和Cu^(2+)所有毒性比例的联合暴露实验都对斑马鱼胚胎致死效应表现为拮抗作用。红外光谱结果对比表明:萘普生和Cu^(2+)之间能发生配合反应,所产生的配合物使二元体系联合毒性降低。展开更多
基金the Key Laboratory of Marine Resource Chemistry and Food Technology(TUST)the Ministry of Education(No.EMTUST-21-08)the Guilin Science and Technology Project(No.20210225-4)。
文摘Deltamethrin(DEL),a commonly used pyrethroid pesticide,results in higher reactive oxygen species(ROS)levels in aquatic animals,which consequently unbalance the redox state.Phlorizin(PHL)is a flavonoid and a natural product promising to prevent or reduce pesticide-induced oxidative stress.Artemia is a micro-crustacean widely used in marine hatcheries and an experimental aquatic organism for environmental toxicology research.This research aimed to evaluate the toxicity of DEL on Artemia and the antioxidative effect of PHL against the toxicity.Results show that 0.08-mg/mL PHL exerted its antioxidative effects on hatching percentage of the cysts in 24-h incubation and on body length and survival rate of Artemia in 12-d culture.After 12-d culture,12-,24-,and 36-h DEL exposure showed significant drops in SOD,CAT,and GSH-Px enzyme activities,and significant increases in ROS and malondialdehyde(MDA)levels in Artemia(P<0.05).On the contrary,0.08-mg/mL PHL application improved the enzyme activities and decreased the ROS and MDA levels(P<0.05).Moreover,0.08-mg/mL PHL significantly increased mRNA expression levels of Cu/Zn SOD,CAT,GST,HO-1,NQO1,and Nrf2,and decreased mRNA expression level of Keap1 in the DEL-exposed Artemia(P<0.05).Therefore,DEL is toxic to Artemia,while PHL alleviates DEL-induced oxidative damage by possibly regulating the Nrf2signaling pathway.This study provided a theoretical basis for PHL to reduce pesticide-induced toxicity in aquatic animals.
文摘萘普生的广泛使用导致其在环境中持续输入与积累,对生态环境造成潜在的危害。金属离子Cu^(2+)虽然在自然界中普遍存在,但是萘普生和Cu^(2+)的联合毒性效应目前尚不清楚。开展了毒性预实验,根据Cu^(2+)和萘普生96 h LC 50值(毒性单位比4∶1,2∶1,1∶1,1∶2,1∶4)对斑马鱼胚胎进行混合暴露,采用相加指数(AI)法评价联合毒性影响。实验结果表明:萘普生和Cu^(2+)单独暴露对斑马鱼胚胎产生发育毒性,萘普生和Cu^(2+)所有毒性比例的联合暴露实验都对斑马鱼胚胎致死效应表现为拮抗作用。红外光谱结果对比表明:萘普生和Cu^(2+)之间能发生配合反应,所产生的配合物使二元体系联合毒性降低。
