Aim To study the relationship between the changes of ecology and environments and the transmission of Schistosomiasis japanica after the construction of the three gorges reservoir.Methods Based on predictive data supp...Aim To study the relationship between the changes of ecology and environments and the transmission of Schistosomiasis japanica after the construction of the three gorges reservoir.Methods Based on predictive data supplied by the Yangtze River Committee on Water Conservancy,of the changes of the water level and sediment in the middle and lower reaches of the Yangtze river after the dam construction, corresponding data were collected and mathematics model was extablished by field pilot studies. In order to understand the effects of changes of water level and sediment on the growth 4 distribution of Oncomelania hupensis which is the intermediate host of Schistosoma japonicum, field surveys and ecologically intimated experiments were done.The prevalence status and factors related to transmission were investigated by methods of epidemiology, geographical medicine and social medicine.Results 1 The Three Gorges Project is an integrated project in a large scale to exploit and improve the environments of the Yangtze River. It will have a profound effect on improving and accelerating China’s social and economic development. 2 Positive impact on control of Schistosomiasis after the construction of The Three Gorges Reservoir:After the dam construction, the decrease in the frequency of flooding in Yangtze River will be occurred which cease the diffusion of snails and decline the chances ofinfectionofhumanandlivestock. Snails habitats will be reduced in the flushed areas along the Yangtze River. Exploitation of beaches and delimination of snails will be benefited from the beach’s uplift in the flushed areas. And the floods that will be ahead of time in autumn will interfere the development of snails.3 Negative impact on control of Schistosomiasis after the construction of The Three Gorges Reservoir:The flushed beaches and migrator’s settlements in certain altitude may become snail habitats.The reservoir areas will become the potential transmission areas of Schistosomiasis due to diffusion of infectious resources and Oncomelania snails. The uprising of the water body level may cause the expending of the snails habitats in some place of Jianghan plain in between spring and summer.The perish of Dongting lake will be put off by the delayed flush, which causes the beach in a situation of growing reed-grass or grass for a long time that benefited to snails’ reproduction. And the chances for human and livestock being infected were increased because of the ahead of time of water regression in Dongting beach in autumn.The distribution of snails will not be affected by the changes of water level in the beaches of the downstream of Yangtze River after the dam construction, but the flushed section in Anhui in recent 80 years are suitable for the snails development.4 The environmental changes due to dam construction will not obviously affect the development of snails and the transmission of Schistosomiasis in the Poyang Lake.5 This report is an elementary study and further researches are needed.Conclusion The changes of ecology and environment after dam construction will have positive and negative impact on the transmission of Schistosomiasis in the Three Gorges area and the middle and lower reaches of the Yangtze River.We should consider the negative influences and more studies are needed in making strategies for controlling the transmission of Schistosomiasis. [展开更多
目的构建亚洲带绦虫成虫全长cDNA质粒文库,为获取亚洲绦虫成虫的基因信息,建立基因表达谱,并为筛选疫苗基因和诊断抗原基因奠定基础。方法提取亚洲带绦虫成虫mRNA,构建pBluescript II SK全长cDNA质粒文库,测定扩增文库的滴度;用载体克...目的构建亚洲带绦虫成虫全长cDNA质粒文库,为获取亚洲绦虫成虫的基因信息,建立基因表达谱,并为筛选疫苗基因和诊断抗原基因奠定基础。方法提取亚洲带绦虫成虫mRNA,构建pBluescript II SK全长cDNA质粒文库,测定扩增文库的滴度;用载体克隆位点两端的引物进行PCR扩增,以检测所构建文库的质量。随机挑选质粒文库转化的阳性重组克隆,进行较大规模的5′端测序,归并unigene。结果测定文库的滴度为1011pfu/μl,插入片段的大小主要在1000bp以上。共测1495个克隆,获得有效EST序列1126个,归并为643条unigene。结论已成功获得一高质量的亚洲带绦虫成虫全长cDNA表达文库,并获得了较丰富的成虫表达基因数据。展开更多
文摘Aim To study the relationship between the changes of ecology and environments and the transmission of Schistosomiasis japanica after the construction of the three gorges reservoir.Methods Based on predictive data supplied by the Yangtze River Committee on Water Conservancy,of the changes of the water level and sediment in the middle and lower reaches of the Yangtze river after the dam construction, corresponding data were collected and mathematics model was extablished by field pilot studies. In order to understand the effects of changes of water level and sediment on the growth 4 distribution of Oncomelania hupensis which is the intermediate host of Schistosoma japonicum, field surveys and ecologically intimated experiments were done.The prevalence status and factors related to transmission were investigated by methods of epidemiology, geographical medicine and social medicine.Results 1 The Three Gorges Project is an integrated project in a large scale to exploit and improve the environments of the Yangtze River. It will have a profound effect on improving and accelerating China’s social and economic development. 2 Positive impact on control of Schistosomiasis after the construction of The Three Gorges Reservoir:After the dam construction, the decrease in the frequency of flooding in Yangtze River will be occurred which cease the diffusion of snails and decline the chances ofinfectionofhumanandlivestock. Snails habitats will be reduced in the flushed areas along the Yangtze River. Exploitation of beaches and delimination of snails will be benefited from the beach’s uplift in the flushed areas. And the floods that will be ahead of time in autumn will interfere the development of snails.3 Negative impact on control of Schistosomiasis after the construction of The Three Gorges Reservoir:The flushed beaches and migrator’s settlements in certain altitude may become snail habitats.The reservoir areas will become the potential transmission areas of Schistosomiasis due to diffusion of infectious resources and Oncomelania snails. The uprising of the water body level may cause the expending of the snails habitats in some place of Jianghan plain in between spring and summer.The perish of Dongting lake will be put off by the delayed flush, which causes the beach in a situation of growing reed-grass or grass for a long time that benefited to snails’ reproduction. And the chances for human and livestock being infected were increased because of the ahead of time of water regression in Dongting beach in autumn.The distribution of snails will not be affected by the changes of water level in the beaches of the downstream of Yangtze River after the dam construction, but the flushed section in Anhui in recent 80 years are suitable for the snails development.4 The environmental changes due to dam construction will not obviously affect the development of snails and the transmission of Schistosomiasis in the Poyang Lake.5 This report is an elementary study and further researches are needed.Conclusion The changes of ecology and environment after dam construction will have positive and negative impact on the transmission of Schistosomiasis in the Three Gorges area and the middle and lower reaches of the Yangtze River.We should consider the negative influences and more studies are needed in making strategies for controlling the transmission of Schistosomiasis. [
文摘目的构建亚洲带绦虫成虫全长cDNA质粒文库,为获取亚洲绦虫成虫的基因信息,建立基因表达谱,并为筛选疫苗基因和诊断抗原基因奠定基础。方法提取亚洲带绦虫成虫mRNA,构建pBluescript II SK全长cDNA质粒文库,测定扩增文库的滴度;用载体克隆位点两端的引物进行PCR扩增,以检测所构建文库的质量。随机挑选质粒文库转化的阳性重组克隆,进行较大规模的5′端测序,归并unigene。结果测定文库的滴度为1011pfu/μl,插入片段的大小主要在1000bp以上。共测1495个克隆,获得有效EST序列1126个,归并为643条unigene。结论已成功获得一高质量的亚洲带绦虫成虫全长cDNA表达文库,并获得了较丰富的成虫表达基因数据。
