Background and objectives: Diabetes is a chronic multifactorial disease which requires a variety of strategies to reduce its epidemic. Type 2 diabetes (T2MD) is the most common form of diabetes which results from inef...Background and objectives: Diabetes is a chronic multifactorial disease which requires a variety of strategies to reduce its epidemic. Type 2 diabetes (T2MD) is the most common form of diabetes which results from inefficiency of insulin secretion or resistance to insulin action, both of which lead to chronic hyperglycemia. Lipid peroxidation is an oxidative stress process that involve in T2DM complications. This study aimed to 1) determine plasma malondialdehyde (MDA) levels as a biomarker for lipid peroxidation in Sudanese patients with T2DM, and 2) assess the associations between MDA and diabetes-related variables. Material and Methods: This case-control study was conducted from November 2022 to June 2023 at the National University, Sudan. It included 100 participants, of whom 50 were T2DM patients and 50 were healthy controls. Data on demographics, clinical characteristics, and biochemical markers (FBS, HbA1c, and MDA) were collected. The NycoCard HbA1c method and the GOD-POD Method were used for HbA1c and glucose measurement, respectively. MDA was determined by the thiobarbituric acid reactive substances method. The data were analyzed using SPSS Version. Results: Significant differences were observed between T2DM patients and healthy controls in FBS (P = 0.000), HbA1c (P = 0.000), and MDA (P = 0.010), with T2DM patients exhibiting higher levels. The study revealed a strong positive correlation between MDA levels and the duration of diabetes (r = 0.69, P = 0.00), while other variables, including age, BMI, and glucose control, did not significantly correlate with MDA levels. Conclusion: Findings revealed elevated MDA levels in Sudanese T2DM patients, with a positive correlation between MDA and diabetes duration. These findings emphasize the importance of oxidative stress in T2DM pathogenesis and call for the need for targeted strategies to mitigate oxidative damage and improve diabetes care.展开更多
The paper was to explore the effect of rice blast resistance induced by Ag-antibiotic 702 on antioxidant enzyme activities and malondialdehyde( MDA)content. At three-leaf and one-heart stage of Luliangyou 996,15 μg/m...The paper was to explore the effect of rice blast resistance induced by Ag-antibiotic 702 on antioxidant enzyme activities and malondialdehyde( MDA)content. At three-leaf and one-heart stage of Luliangyou 996,15 μg/mL Ag-antibiotic 702 was sprayed,while Validamycin and distilled water were sprayed as positive control and negative control,respectively. The antioxidant enzyme( SOD,POD,PPO,CAT and PAL) activities and malondialdehyde( MDA) content in rice leaves were determined every 24 h within 168 h post spraying. Compared with CK,the MDA content and activities of the five antioxidant enzymes increased in Agantibiotic 702 treatment,Validamycin treatment and M. grisea inoculation treatment. The MDA content and SOD,CAT and POD activities in Ag-antibiotic 702 treatment were significantly higher than those in M. grisea inoculation treatment in early stage,and the APX and LOX activities increased more remarkably and rapidly. The induction effect of Ag-antibiotic 702 could reach or exceed that of Validamycin treatment. Ag-antibiotic 702 enhanced the resistance to rice blast because MDA content and antioxidant enzymes activities were improved in rice leaves. Therefore,the increase of rice blast resistance induced by Ag-antibiotic 702 in rice was positively correlated with the increase of MDA content and antioxidant enzymes activities in rice leaves.展开更多
Objective:To investigate effects of in ovo ghrelin administration on serum malondialdehyde(MDA)level in newly-hatched chickens.Methods:Fertilized eggs were divided into 7 groups:group Tl as control(without injection),...Objective:To investigate effects of in ovo ghrelin administration on serum malondialdehyde(MDA)level in newly-hatched chickens.Methods:Fertilized eggs were divided into 7 groups:group Tl as control(without injection),group T2(in ovo injected with 50 ng/egg ghrelin on day 5),group T3(in ovo injected with 100 ng/egg ghrelin on day 5),group T4(in ovo injected with 50 ng/egg ghrelin on day 10),group T5(in ovo injected with 100 ng/egg ghrelin on day 10),group T6(in ovo injected with solvent:1%acetic acid,without ghrelin on day 5)and group T7(in ovo injected with solvent without ghrelin on day 10).After hatching,serum MDA concentrations were determined.Results:Chrelin administrated groups(T2,T3,T4 and T5)had lower serum MDA level in comparison with control group(T1)or solvent injected groups(T6 and T7).T2 and T3(ghrelin injection on day 5)had significantly lower MDA concentrations(4.10 and 4.60 nmol/mL,respectively)in comparison with other groups.In T4 and T5,MDA levels were lower than T1,T6 and T7(non-ghrelin administrated groups)(9.53 and 9.50 in comparison with 10.73,10.03 and 10.13 nmol/mL)and were higher than T2and T3.Conclusions:It can be concluded that in ovo administration of ghrelin can have antioxidative protection and reduce serum MDA level.Ghrelin administration on day 5 of incubation is more efficient.展开更多
AIM: To evaluate αB-crystallin malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GPx) changes in X-ray irradiated rat lens. METHODS: Eight-week-old Sprague-Dawley male rats received X-ray ...AIM: To evaluate αB-crystallin malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GPx) changes in X-ray irradiated rat lens. METHODS: Eight-week-old Sprague-Dawley male rats received X-ray irradiation to the head with rest of the body protected. The exposure dose ranged from 2 to 25 Grays (Gy). The cataract status were examined by slit lamp and rated with "four-grade systems" post-irradiation. The lens MDA level, and the activities of SOD and GPx were measured in a short-term experiment post-irradiation, and αB-crystallin protein levels were quantified. RESULTS: The lenses of normal control and the X-ray irradiated groups with the dose up to 10 Gy remained transparent throughout the experiment. The lens first appeared tiny scatters, and even lamellar opacities in the posterior capsule 45 days post-irradiation with the dose of 15 Gy, and progressed slowly to the advance stage of cataract; while, for the higher dose (25 Gy), the opacity of lens appeared much earlier, and progressed more rapidly to mature stage of cataract within 1 month. At the end of the observation (90 days post-irradiation), almost all lenses became complete opacity with the higher dose (25 Gy). The degree of lens opacity was rated accordingly. The lens MDA level was increased, and SOD and GPx activities were decreased with a dose-dependent manner post-irradiation. The αB-crystallin protein level was decreased dose-dependently at the end point of observation. CONCLUSION: Oxidative events and αB-crystallin may play important roles in the pathogenesis of cataract in X-ray irradiated rat lens.展开更多
Objective:To evaluate the ameliorative effect of melatonin on sub-chronic chlorpyrifos(CPF)and cypermethrin(CYP)-evoked cognitive changes in male Wistar rats.Methods:Fifty adult male Wistar rats,divided into five grou...Objective:To evaluate the ameliorative effect of melatonin on sub-chronic chlorpyrifos(CPF)and cypermethrin(CYP)-evoked cognitive changes in male Wistar rats.Methods:Fifty adult male Wistar rats,divided into five groups of ten rats each,were used for the study.GroupsⅠandⅡwere given distilled water and soya oil(2 ml/kg)respectively.GroupⅢwas administered with melatonin at 0.5 mg/kg only.GroupⅣwas administered with CPF[7.96 mg/kg(1/10th LD_(50))]and CYP[29.6 mg/kg(1/10th LD_(50))].and Group V was administered with CPF[7.96 mg/kg(1/10th LD_(50)]and CYP[29.6 mg/kg(1/10th LD_(50)]30 min after melatonin(0.5 mg/kg).The regimens were administered by gavage once daily for 12 weeks.Thereafter,cognitive performances were determined and the brain was evaluated for malonaldehyde concentration.Results:CPF and CYP induced cognitive deficits and increased brain malonaldehyde concentration,which were all ameliorated by melatonin.Conclusion:Cognitive deficits elicited by CPF and CYP was mitigated by melatonin due to its antioxidant property.展开更多
Objective:To determine the level of oxygen-nitrogen stress parameters in the pathogenesis of amebiasis.Methods:Twenty-four acute intestinal amebiasis patients and 20 healthy controls were enrolled in the present study...Objective:To determine the level of oxygen-nitrogen stress parameters in the pathogenesis of amebiasis.Methods:Twenty-four acute intestinal amebiasis patients and 20 healthy controls were enrolled in the present study.Serum malondialdehyde and nitric oxide levels were determined spectrophotometrically.Results:Serum raalondialdehyde and nitric oxide levels were significantly higher in acute intestinal amebiasis patients than healthy controls(P<0.00l). Conclusions:These results suggest that oxidative and nitrosative stress may play a major role in tissue damage in acute intestinal amebiasis patients.Also these parameters can be used to supplement the conventional microscopic method for reliable diagnosis of intestinal amebiasis.展开更多
Here are reported the changes of superoxide dismutase(SOD)activity andmalondialdehyde(MDA)in the smooth muscle cells of human fetal aorta cultured in vitro with lowdensity lipoprotein(LDL)conditional medium.The result...Here are reported the changes of superoxide dismutase(SOD)activity andmalondialdehyde(MDA)in the smooth muscle cells of human fetal aorta cultured in vitro with lowdensity lipoprotein(LDL)conditional medium.The results showed that a single concentration of hu-man LDL(50μg/ml)stimulated proliferation of smooth muscle cells,and the SOD activityof the cells in the experimental group was higher,from the first to the fifth cultured day whenthe cells had a active proliferation,than that of the control cells.This suggests that LDL might in-duce the increase of SOD activity.At the seventh day,as the cells were in inactive proliferation,SOD activity was low and the difference was significant as compared with that at the fifth day ofthe same group.This also indicates that the SOD activity may be related to the cell proliferation.MDA level within the cells of the esperimental group was lowered with the cell active proliferationand the increase of SOD activity,but when the cells were in inactive proliferation and the SOD ac-tivity decreased,it will remained low.展开更多
Background: Malondialdehyde(MDA) is a candidate general marker of oxidative stress(OS). We sought to assess the relation of MDA to Gulf War illness(GWI) and to a variety of exposures.Methods: This is an observational ...Background: Malondialdehyde(MDA) is a candidate general marker of oxidative stress(OS). We sought to assess the relation of MDA to Gulf War illness(GWI) and to a variety of exposures.Methods: This is an observational study involving subjects from Southern California recruited from October 2011 to May 2014. MDA was assessed in 81 participants(41 GWI-cases, 40 controls). General and Gulf-specific exposures were elicited. MDA case–control comparison was restricted to 40 matched pairs. The potential association between MDA and exposures was assessed using regression analyses. Gulf-specific exposures were incorporated into a case-specific model.Results: Plasma MDA was significantly lower in GWI-cases than controls. Composite pesticide and fuel-solvent exposures negatively predicted MDA in the total sample, as well as in the analyses that included either GWI-cases or controls only. Self-reported exposure to organophosphate(OP) nerve gas was a strong predictor for lower MDA level in veterans with GWI.Conclusions: Past pesticide exposures predicted lower MDA in both veterans with GWI and in healthy controls.展开更多
To observe changes in activity of superoxide dismutase (SOD) and content of malondialdehyde (MDA) in rats with monocrotaline-induced pulmonary hypertension. Methods: Adult ma1e Sprague-Dawley rats were given a single ...To observe changes in activity of superoxide dismutase (SOD) and content of malondialdehyde (MDA) in rats with monocrotaline-induced pulmonary hypertension. Methods: Adult ma1e Sprague-Dawley rats were given a single subcutaneous injection of monocrotaline (MCT, 60 mg/kg) for modeling PH. Activities of SOD and contents of MDA in plasma and pulmonary homogenate were measured by colorimetric analysis. The thickness of the media of pulmonary arterioles (external diameter <100μm) was measured using colour image analysis system. Results: Four weeks after injection of MCT, activities of SOD in venous plasma and pulmonary homogenate for MCT group were 106±45 NU/ml (P<0.05) and 317±59 NU/ml (P<0.01) respectively, whileactivities of SOD for control group were 159±28 NU/ml (P<0.05) and 505±47 NU/ml (P<0.01) respectively.COntents of MDA in venous plasma and pulmonary homogenate for MCT group were 15±5 and 59±14 μmol/L,while contents of MDA for control group were 5. 3±2. 8 and 32±19 ±mol/L. The thickness of the media of pulmonary arterioles increased significantly. Conclusion: The primary cause of PH is the injury of pulmonary vascular endothelial cells by MCT, which decreases the O2 removing ability of the lungs but increases lipid peroxidation,thus inducing PH.展开更多
After Modification of low density lipoprotein(LDL) by either malondialdehyde(MDA) or Cu2+,its thiobarbituric acid reactive substances(TBARS),lipid conjugated dienes,and relative electrophoretic mobility(REM) levels,an...After Modification of low density lipoprotein(LDL) by either malondialdehyde(MDA) or Cu2+,its thiobarbituric acid reactive substances(TBARS),lipid conjugated dienes,and relative electrophoretic mobility(REM) levels,and the fluorescence emission spectra we展开更多
Introduction: Repeated blood transfusions with increased intestinal iron absorption in thalassemia causes iron overload, catalyzed production of various reactive oxygen species subsequently trigger oxidative stress. E...Introduction: Repeated blood transfusions with increased intestinal iron absorption in thalassemia causes iron overload, catalyzed production of various reactive oxygen species subsequently trigger oxidative stress. Endothelial as the first organ exposed to reactive oxygen species would suffer a marked increase in adhesion molecule due to endothelial dysfunction that will trigger a hypercoagulation. Objective: To investigate the correlation between oxidative stress status (malondialdehyde, MDA) and endothelial dysfunction (serum Vascular Cell Adhesion Molecule-1, sVCAM) in iron overload pediatric thalassemic patients with hypercoagulation. Methods: A cross-sectional study was conducted on thalassemic subjects with inclusion criteria: aged 5 - 14 years with iron overload who had hypercoagulation based on TEG (Throm-boelastography) examination. The determination of thalassemia degree was based on a Novel scoring system. The correlation between serum MDA and sVCAM-1 was analyzed using Rank Spearman, with a significance value of 5% and 95% confidence intervals. Results: Nineteen male and twelve female children were included in the study. All subjects had pretransfusion Hb p = 0.02, r = 0.416). The results also showed that there was a significant difference between serum MDA and thalassemia degree (p = 0.029, p = 0.026). Conclusions: Serum MDA level as stress oxidative status marker has moderately positive correlation with sVCAM-1 as endothelial dysfunction marker in iron overloaded pediatric thalassemic patients with hypercoagulation.展开更多
Malondialdehyde (MDA) is the final product of lipid peroxidation, and MDA content can reflect the stress tolerance of plants. To map QTLs conditioning the MDA content in rice leaves, a recombinant inbred line (RIL) po...Malondialdehyde (MDA) is the final product of lipid peroxidation, and MDA content can reflect the stress tolerance of plants. To map QTLs conditioning the MDA content in rice leaves, a recombinant inbred line (RIL) population with 247 lines derived from an indica-indica cross Zhenshan 97B×Milyang 46, and a linkage map consisting of 207 DNA markers were used. The RIL population showed a transgressive segregation in the MDA content of rice leaves. Two QTLs for the MDA content in rice leaves were detected in the intervals RG532-RG811 and RG381-RG236 on chromosome 1, with the additive effects from maternal and paternal parents, accounting for 4.33% and 4.62% of phenotype variations, respectively.展开更多
AIM: To unravel the possible association of malon-dialdehyde (MDA) and fructosamine in anemic H pylori infected patients and to observe the alteration in MDA and fructosamine levels in these patients after treatment f...AIM: To unravel the possible association of malon-dialdehyde (MDA) and fructosamine in anemic H pylori infected patients and to observe the alteration in MDA and fructosamine levels in these patients after treatment for one month.METHODS: Fructosamine, MDA and glucose were estimated in 22 anemic H pylori infected patients and 16 healthy controls. Hematological parameters were also evaluated in both the groups using Sysmex-K-100 automated cell counter. The H pylori infected patients were randomly divided into two groups. H pylori infected patients in GroupⅠreceived both iron supplementation and anti-H pylori therapy, while patients in Group Ⅱ received only iron supplementation. All the biochemical and hematological parameters were estimated after one month of treatment. RESULTS: In anemic H pylori infected patients, while MDA (5.41 ± 2.16 vs 2.26 ± 0.50; P < 0.05) and fructosamine (2.64 ± 0.93 vs 1.60 ± 0.35; P < 0.05) were significantly increased, iron (32.72 ± 14.93 vs 110.25 ± 26.58; P < 0.05), hemoglobin (6.9 ± 2.6 vs 12.66 ± 0.74; P < 0.05) and ferritin (28.82 ± 16.27 vs 140.43 ± 30.72; P < 0.05) levels were significantly decreased compared with the controls. With partial correlation analysis, fructosamine was found to have a significant positive correlation with MDA. In GroupⅠ, while MDA level decreased significantly (3.11 ± 1.73 vs 5.50 ± 2.46; P < 0.05), there was a significant increase in iron (84.09 ± 29.51 vs 36.09 ± 17.81; P < 0.05), hemoglobin (10.40 ± 1.11 vs 7.42 ± 1.90; P < 0.05) and ferritin (116.91 ± 63.34 vs 30.46 ± 17.81; P < 0.05) levels after one month. There was no significant change in the levels of fructosamine in groupⅠafter treatment. Similarly, no significant alterations were noted in the levels of MDA, fructosamine, hemoglobin or ferritin in Group Ⅱ patients after one month of treatment.CONCLUSION: An increased level of fructosamine and MDA was found in anemic H pylori infected patients. Present data supports the premise that lipid peroxides per se do play a role in the glycation of plasma proteins. Furthermore, the findings from this study indicate that treatment for both anemia and H pylori infections is required for lowering the levels of lipid peroxides in these patients.展开更多
Under global cerebral ischemia, the effect of different brain temperature on cerebral ischemic injury was studied. Male Sprague-Dawley rats were divided into normothermic(37–38°C) ischemia, mild hypothermic(31–...Under global cerebral ischemia, the effect of different brain temperature on cerebral ischemic injury was studied. Male Sprague-Dawley rats were divided into normothermic(37–38°C) ischemia, mild hypothermic(31–32°C) ischemia, hyperthermic(41–42°C) ischemia and sham-operated groups. Global cerebral ischemia was established using the Pulsinelli four-vessel occlusion model and brain temperature was maintained at defined level for 60 min after 20-min ischemia. The expression of c-fos protein and the levels of malondialdehyde(MDA) and lactate in brain regions were detected by immunochemistry and spectrophotometrical methods, respectively. C-fos positive neurons were found in the hippocampus and cerebral cortex after cerebral ischemia reperfusion. Mild hypothermia increased the expression of c-fos protein in both areas, whereas hyperthermia decreased the expression of c-fos protein in the hippocampus at 24 h reperfusion, and the cerebral cortex at 48 h reperfusion when compared to normothermic conditions. In normothermic, mild hypothermic and hyperthermic ischemia groups, the levels of MDA and lactate in brain tissue were increased at 24, 48 and 72 h reperfusion following 20-min ischemia as compared with the sham-operated group(P<0.01). The levels of MDA and lactate in mild hypothermic group were significantly lower than those in normothermic group(P<0.01). It is suggested that brain temperature influences the translation of the immunoreactive protein product of c-fos after global cerebral ischemia, and MDA and lactate are also affected by hypothermia and hyperthermia.展开更多
Effect of Siqi decoction on myocardial ischemia is to prevent cardiac myocyte membrane from damage associated with oxygen free radicals related to NO. To research the regulatoin of the content of malondialdehyde by Si...Effect of Siqi decoction on myocardial ischemia is to prevent cardiac myocyte membrane from damage associated with oxygen free radicals related to NO. To research the regulatoin of the content of malondialdehyde by Siqi decoction, an index of lipid peroxidation, via increasing activity of superoxide dismutase in blood serum of rats with Myocardial Ischemia, the model of myocardium ischemia was made in Wistar rats with posterior pituitary injection through vein in tail. Siqi decoction, Diaoxinxuekang(DK) and Fufangdanshenpian(FD), the latter two drugs of which are effective TCM drugs of anti-myocardial ischemia at present, were administrated to the rats with myocar-dium ischemia for 5 days to compare the effect of them on myocardium ischemia as reference drugs via measuring the changes of the content of malondialdehyde and the activity of superoxide dismutase in the rat blood serum with myocardial ischemia. There were a remarkable increase in the activity of superoxide dismutase and a decrease in the content of malondialdehyde in the serum of the rats administered Siqi decoction compared with those of the rats in control group, p<0.05. The contents of MDA in the serum of the prevention group rats in the experiments are lower than those of the cure group rats. Anti-Myocardium Ischemia mechanism of Siqi decoction is the regulation of the content of malondialdehyde via increasing activity of superoxide dismutase in the serum of Rats with myocardial ischemia and stimulating the activity of NOS in serum so as to increase NO concentration.展开更多
The antioxidant enzyme activity and malondialdehyde(MDA) content of Cephalothrix hongkongiensis were studied to assess variations in the biochemical/physiological parameters of nemerteans under heavy metal stress.Worm...The antioxidant enzyme activity and malondialdehyde(MDA) content of Cephalothrix hongkongiensis were studied to assess variations in the biochemical/physiological parameters of nemerteans under heavy metal stress.Worms were exposed to copper,zinc and cadmium solutions at different concentrations,and the activity of three antioxidant enzymes,catalase(CAT),superoxide dismutase(SOD),and glutathione peroxidase(GPX),and MDA content were measured.The results show that the activity of each enzyme changed immediately after exposure to heavy metals.CAT was invariably inhibited throughout the experimental period,while the SOD activity was significantly elevated by exposure to Cu^(2+) for 48h,but then decreased.SOD was inhibited by Zn^(2+) during the first 12h of exposure,but activated when exposed for longer periods.Under Cd^(2+) stress,SOD activity decreased within 72h.GPX activity varied greatly,being significantly increased by both Cu^(2+) and Zn^(2+),but significantly inhibited by Cd^(2+) in the first 12-24h after exposure.MDA content increased on Cu^(2+) exposure,but normally decreased on Zn^(2+) exposure.MDA content followed an increase-decrease-increase pattern under Cd^(2+) stress.In conclusion,the antioxidant system of this nemertean is sensitive to heavy metals,and its CAT activity may be a potential biomarker for monitoring heavy metal levels in the environment.展开更多
AIM: To study the protective effect of a natural antioxi- dant, melatonin, against multistress condition induced lipid peroxidation via determination of gastric damage and plasma malondialdehyde (MDA) level by high pe...AIM: To study the protective effect of a natural antioxi- dant, melatonin, against multistress condition induced lipid peroxidation via determination of gastric damage and plasma malondialdehyde (MDA) level by high perfor- mance liquid chromatography in rats. METHODS: We compared indomethacin-induced gas- tric damage and MDA plasma level in three groups of rats: unoperated, bile duct ligated and sham-operated and evaluated the role of the melatonin on gastric dam- age and plasma MDA level. Indomethacin and melatonin were injected intraperitoneally in doses of 50 mg/kg and 20 mg/kg, respectively. Animals were killed 4 h after in- domethacin injection. RESULTS: Indomethacin induced more severe gastric damage and plasma MDA level in bile duct ligated ani- mals was significantly higher (3.1 ± 0.04 μmol/L) than sham (2.8 ± 0.04 μmol/L) and unoperated animals (1.4 ± 0.08 μmol/L). Pretreatment with melatonin reduced indomethacin-induced gastric damage and plasma MDA level. CONCLUSION: Considering the results of this study, we suggest that in multistress conditions the intensity of gastric damage and the plasma MDA level are great and melatonin reduces the negative effect of lipid peroxida- tion and cell damage by oxidative stress in multistress conditions due to its antioxidizing activity.展开更多
[Objective] To investigate the relationship between perinatal diseases and contents of superoxide dismutase (SOD) and malondialdehyde (MDA) in serum of dairy cows. [Method] A total of 38 dairy cows including 22 health...[Objective] To investigate the relationship between perinatal diseases and contents of superoxide dismutase (SOD) and malondialdehyde (MDA) in serum of dairy cows. [Method] A total of 38 dairy cows including 22 healthy cows, five cows with ketosis, four cows with fatty liver and seven cows with retained fatal placenta in perinatal period were selected. The contents of SOD and MDA in serum were respectively determined at different times ante partum and post partum. [Result] In the dairy cows with perinatal diseases, the SOD activity is lower and the MDA content was higher than that in the healthy dairy cows during perinatal period. [Conclusion] The antioxidant activity of dairy cows with perinatal diseases is lower than that of healthy dairy cows during perinatal period.展开更多
Objective: To determine the concentration of malondialdehyde (MDA), product of lipid peroxidation, in seminal plasma and evalu ate its significance. Methods: Ninety-three cases of infertile pa tients were divided into...Objective: To determine the concentration of malondialdehyde (MDA), product of lipid peroxidation, in seminal plasma and evalu ate its significance. Methods: Ninety-three cases of infertile pa tients were divided into the obstructive azoospermic (12 cases), the non-obstructive azoospermic (15 cases), the oligozoospermic (21 cases), the asthenozoospermic (19 cases), the oligoastheno zoospermic (16 cases) and the oligoasthenoteratozoospermic (10) groups. Eighteen fertile males served as the controls. MDA con centration was assessed by high-performance liquid chromatogra phy (HPLC). Results: With the exception of the obstructive azoospermic group, the MDA concentration in the seminal plasma was significantly different between the control group and the infer tile groups (P<0.01) as well as between the different infertile groups. Conclusion: Determination of MDA concentration in the seminal plasma is helpful to the diagnosis of male infertility induced by overproduction of reactive oxygen species.展开更多
文摘Background and objectives: Diabetes is a chronic multifactorial disease which requires a variety of strategies to reduce its epidemic. Type 2 diabetes (T2MD) is the most common form of diabetes which results from inefficiency of insulin secretion or resistance to insulin action, both of which lead to chronic hyperglycemia. Lipid peroxidation is an oxidative stress process that involve in T2DM complications. This study aimed to 1) determine plasma malondialdehyde (MDA) levels as a biomarker for lipid peroxidation in Sudanese patients with T2DM, and 2) assess the associations between MDA and diabetes-related variables. Material and Methods: This case-control study was conducted from November 2022 to June 2023 at the National University, Sudan. It included 100 participants, of whom 50 were T2DM patients and 50 were healthy controls. Data on demographics, clinical characteristics, and biochemical markers (FBS, HbA1c, and MDA) were collected. The NycoCard HbA1c method and the GOD-POD Method were used for HbA1c and glucose measurement, respectively. MDA was determined by the thiobarbituric acid reactive substances method. The data were analyzed using SPSS Version. Results: Significant differences were observed between T2DM patients and healthy controls in FBS (P = 0.000), HbA1c (P = 0.000), and MDA (P = 0.010), with T2DM patients exhibiting higher levels. The study revealed a strong positive correlation between MDA levels and the duration of diabetes (r = 0.69, P = 0.00), while other variables, including age, BMI, and glucose control, did not significantly correlate with MDA levels. Conclusion: Findings revealed elevated MDA levels in Sudanese T2DM patients, with a positive correlation between MDA and diabetes duration. These findings emphasize the importance of oxidative stress in T2DM pathogenesis and call for the need for targeted strategies to mitigate oxidative damage and improve diabetes care.
基金Supported by National Natural Science Foundation of China(31360450)
文摘The paper was to explore the effect of rice blast resistance induced by Ag-antibiotic 702 on antioxidant enzyme activities and malondialdehyde( MDA)content. At three-leaf and one-heart stage of Luliangyou 996,15 μg/mL Ag-antibiotic 702 was sprayed,while Validamycin and distilled water were sprayed as positive control and negative control,respectively. The antioxidant enzyme( SOD,POD,PPO,CAT and PAL) activities and malondialdehyde( MDA) content in rice leaves were determined every 24 h within 168 h post spraying. Compared with CK,the MDA content and activities of the five antioxidant enzymes increased in Agantibiotic 702 treatment,Validamycin treatment and M. grisea inoculation treatment. The MDA content and SOD,CAT and POD activities in Ag-antibiotic 702 treatment were significantly higher than those in M. grisea inoculation treatment in early stage,and the APX and LOX activities increased more remarkably and rapidly. The induction effect of Ag-antibiotic 702 could reach or exceed that of Validamycin treatment. Ag-antibiotic 702 enhanced the resistance to rice blast because MDA content and antioxidant enzymes activities were improved in rice leaves. Therefore,the increase of rice blast resistance induced by Ag-antibiotic 702 in rice was positively correlated with the increase of MDA content and antioxidant enzymes activities in rice leaves.
基金Supported by Shabcstar branch,Islamic Azad University,Iran
文摘Objective:To investigate effects of in ovo ghrelin administration on serum malondialdehyde(MDA)level in newly-hatched chickens.Methods:Fertilized eggs were divided into 7 groups:group Tl as control(without injection),group T2(in ovo injected with 50 ng/egg ghrelin on day 5),group T3(in ovo injected with 100 ng/egg ghrelin on day 5),group T4(in ovo injected with 50 ng/egg ghrelin on day 10),group T5(in ovo injected with 100 ng/egg ghrelin on day 10),group T6(in ovo injected with solvent:1%acetic acid,without ghrelin on day 5)and group T7(in ovo injected with solvent without ghrelin on day 10).After hatching,serum MDA concentrations were determined.Results:Chrelin administrated groups(T2,T3,T4 and T5)had lower serum MDA level in comparison with control group(T1)or solvent injected groups(T6 and T7).T2 and T3(ghrelin injection on day 5)had significantly lower MDA concentrations(4.10 and 4.60 nmol/mL,respectively)in comparison with other groups.In T4 and T5,MDA levels were lower than T1,T6 and T7(non-ghrelin administrated groups)(9.53 and 9.50 in comparison with 10.73,10.03 and 10.13 nmol/mL)and were higher than T2and T3.Conclusions:It can be concluded that in ovo administration of ghrelin can have antioxidative protection and reduce serum MDA level.Ghrelin administration on day 5 of incubation is more efficient.
基金Scientific Research Foundation for Returned Scholars, the Second Hospital Affiliated to Soochow University (No.SDFEY-2007-10)National Natural Science Foundation of China (No.81000383)+2 种基金Research Fund for the Doctoral Program of Higher Education of China (No.20100072120051)Program of Tongji University (No.1500219024 No.2010QH04 and No. 2010YF02)
文摘AIM: To evaluate αB-crystallin malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GPx) changes in X-ray irradiated rat lens. METHODS: Eight-week-old Sprague-Dawley male rats received X-ray irradiation to the head with rest of the body protected. The exposure dose ranged from 2 to 25 Grays (Gy). The cataract status were examined by slit lamp and rated with "four-grade systems" post-irradiation. The lens MDA level, and the activities of SOD and GPx were measured in a short-term experiment post-irradiation, and αB-crystallin protein levels were quantified. RESULTS: The lenses of normal control and the X-ray irradiated groups with the dose up to 10 Gy remained transparent throughout the experiment. The lens first appeared tiny scatters, and even lamellar opacities in the posterior capsule 45 days post-irradiation with the dose of 15 Gy, and progressed slowly to the advance stage of cataract; while, for the higher dose (25 Gy), the opacity of lens appeared much earlier, and progressed more rapidly to mature stage of cataract within 1 month. At the end of the observation (90 days post-irradiation), almost all lenses became complete opacity with the higher dose (25 Gy). The degree of lens opacity was rated accordingly. The lens MDA level was increased, and SOD and GPx activities were decreased with a dose-dependent manner post-irradiation. The αB-crystallin protein level was decreased dose-dependently at the end point of observation. CONCLUSION: Oxidative events and αB-crystallin may play important roles in the pathogenesis of cataract in X-ray irradiated rat lens.
基金Supported by Direct Teaching and Laboratory Grant 560599 to the Department of Veterinary Physiology and Pharmacology,Ahmadu Bello University,Zaria,Nigeria
文摘Objective:To evaluate the ameliorative effect of melatonin on sub-chronic chlorpyrifos(CPF)and cypermethrin(CYP)-evoked cognitive changes in male Wistar rats.Methods:Fifty adult male Wistar rats,divided into five groups of ten rats each,were used for the study.GroupsⅠandⅡwere given distilled water and soya oil(2 ml/kg)respectively.GroupⅢwas administered with melatonin at 0.5 mg/kg only.GroupⅣwas administered with CPF[7.96 mg/kg(1/10th LD_(50))]and CYP[29.6 mg/kg(1/10th LD_(50))].and Group V was administered with CPF[7.96 mg/kg(1/10th LD_(50)]and CYP[29.6 mg/kg(1/10th LD_(50)]30 min after melatonin(0.5 mg/kg).The regimens were administered by gavage once daily for 12 weeks.Thereafter,cognitive performances were determined and the brain was evaluated for malonaldehyde concentration.Results:CPF and CYP induced cognitive deficits and increased brain malonaldehyde concentration,which were all ameliorated by melatonin.Conclusion:Cognitive deficits elicited by CPF and CYP was mitigated by melatonin due to its antioxidant property.
基金Supported by Scientific Research Projects Governing Unit ofGaziantep University(No.TF:00 04)
文摘Objective:To determine the level of oxygen-nitrogen stress parameters in the pathogenesis of amebiasis.Methods:Twenty-four acute intestinal amebiasis patients and 20 healthy controls were enrolled in the present study.Serum malondialdehyde and nitric oxide levels were determined spectrophotometrically.Results:Serum raalondialdehyde and nitric oxide levels were significantly higher in acute intestinal amebiasis patients than healthy controls(P<0.00l). Conclusions:These results suggest that oxidative and nitrosative stress may play a major role in tissue damage in acute intestinal amebiasis patients.Also these parameters can be used to supplement the conventional microscopic method for reliable diagnosis of intestinal amebiasis.
文摘Here are reported the changes of superoxide dismutase(SOD)activity andmalondialdehyde(MDA)in the smooth muscle cells of human fetal aorta cultured in vitro with lowdensity lipoprotein(LDL)conditional medium.The results showed that a single concentration of hu-man LDL(50μg/ml)stimulated proliferation of smooth muscle cells,and the SOD activityof the cells in the experimental group was higher,from the first to the fifth cultured day whenthe cells had a active proliferation,than that of the control cells.This suggests that LDL might in-duce the increase of SOD activity.At the seventh day,as the cells were in inactive proliferation,SOD activity was low and the difference was significant as compared with that at the fifth day ofthe same group.This also indicates that the SOD activity may be related to the cell proliferation.MDA level within the cells of the esperimental group was lowered with the cell active proliferationand the increase of SOD activity,but when the cells were in inactive proliferation and the SOD ac-tivity decreased,it will remained low.
基金funded by the Depar tment of Defense Congressionally Directed Medical Research Program (GW093063)。
文摘Background: Malondialdehyde(MDA) is a candidate general marker of oxidative stress(OS). We sought to assess the relation of MDA to Gulf War illness(GWI) and to a variety of exposures.Methods: This is an observational study involving subjects from Southern California recruited from October 2011 to May 2014. MDA was assessed in 81 participants(41 GWI-cases, 40 controls). General and Gulf-specific exposures were elicited. MDA case–control comparison was restricted to 40 matched pairs. The potential association between MDA and exposures was assessed using regression analyses. Gulf-specific exposures were incorporated into a case-specific model.Results: Plasma MDA was significantly lower in GWI-cases than controls. Composite pesticide and fuel-solvent exposures negatively predicted MDA in the total sample, as well as in the analyses that included either GWI-cases or controls only. Self-reported exposure to organophosphate(OP) nerve gas was a strong predictor for lower MDA level in veterans with GWI.Conclusions: Past pesticide exposures predicted lower MDA in both veterans with GWI and in healthy controls.
文摘To observe changes in activity of superoxide dismutase (SOD) and content of malondialdehyde (MDA) in rats with monocrotaline-induced pulmonary hypertension. Methods: Adult ma1e Sprague-Dawley rats were given a single subcutaneous injection of monocrotaline (MCT, 60 mg/kg) for modeling PH. Activities of SOD and contents of MDA in plasma and pulmonary homogenate were measured by colorimetric analysis. The thickness of the media of pulmonary arterioles (external diameter <100μm) was measured using colour image analysis system. Results: Four weeks after injection of MCT, activities of SOD in venous plasma and pulmonary homogenate for MCT group were 106±45 NU/ml (P<0.05) and 317±59 NU/ml (P<0.01) respectively, whileactivities of SOD for control group were 159±28 NU/ml (P<0.05) and 505±47 NU/ml (P<0.01) respectively.COntents of MDA in venous plasma and pulmonary homogenate for MCT group were 15±5 and 59±14 μmol/L,while contents of MDA for control group were 5. 3±2. 8 and 32±19 ±mol/L. The thickness of the media of pulmonary arterioles increased significantly. Conclusion: The primary cause of PH is the injury of pulmonary vascular endothelial cells by MCT, which decreases the O2 removing ability of the lungs but increases lipid peroxidation,thus inducing PH.
文摘After Modification of low density lipoprotein(LDL) by either malondialdehyde(MDA) or Cu2+,its thiobarbituric acid reactive substances(TBARS),lipid conjugated dienes,and relative electrophoretic mobility(REM) levels,and the fluorescence emission spectra we
文摘Introduction: Repeated blood transfusions with increased intestinal iron absorption in thalassemia causes iron overload, catalyzed production of various reactive oxygen species subsequently trigger oxidative stress. Endothelial as the first organ exposed to reactive oxygen species would suffer a marked increase in adhesion molecule due to endothelial dysfunction that will trigger a hypercoagulation. Objective: To investigate the correlation between oxidative stress status (malondialdehyde, MDA) and endothelial dysfunction (serum Vascular Cell Adhesion Molecule-1, sVCAM) in iron overload pediatric thalassemic patients with hypercoagulation. Methods: A cross-sectional study was conducted on thalassemic subjects with inclusion criteria: aged 5 - 14 years with iron overload who had hypercoagulation based on TEG (Throm-boelastography) examination. The determination of thalassemia degree was based on a Novel scoring system. The correlation between serum MDA and sVCAM-1 was analyzed using Rank Spearman, with a significance value of 5% and 95% confidence intervals. Results: Nineteen male and twelve female children were included in the study. All subjects had pretransfusion Hb p = 0.02, r = 0.416). The results also showed that there was a significant difference between serum MDA and thalassemia degree (p = 0.029, p = 0.026). Conclusions: Serum MDA level as stress oxidative status marker has moderately positive correlation with sVCAM-1 as endothelial dysfunction marker in iron overloaded pediatric thalassemic patients with hypercoagulation.
基金supported by the Super Rice Program of Ministry of Agriculture of China (Grant No. 200606)the Key Program of Zhejiang Province, China (Grant No. 2003G10028)the China Postdoctoral Science Foundation (Grant No. 2003034232)
文摘Malondialdehyde (MDA) is the final product of lipid peroxidation, and MDA content can reflect the stress tolerance of plants. To map QTLs conditioning the MDA content in rice leaves, a recombinant inbred line (RIL) population with 247 lines derived from an indica-indica cross Zhenshan 97B×Milyang 46, and a linkage map consisting of 207 DNA markers were used. The RIL population showed a transgressive segregation in the MDA content of rice leaves. Two QTLs for the MDA content in rice leaves were detected in the intervals RG532-RG811 and RG381-RG236 on chromosome 1, with the additive effects from maternal and paternal parents, accounting for 4.33% and 4.62% of phenotype variations, respectively.
文摘AIM: To unravel the possible association of malon-dialdehyde (MDA) and fructosamine in anemic H pylori infected patients and to observe the alteration in MDA and fructosamine levels in these patients after treatment for one month.METHODS: Fructosamine, MDA and glucose were estimated in 22 anemic H pylori infected patients and 16 healthy controls. Hematological parameters were also evaluated in both the groups using Sysmex-K-100 automated cell counter. The H pylori infected patients were randomly divided into two groups. H pylori infected patients in GroupⅠreceived both iron supplementation and anti-H pylori therapy, while patients in Group Ⅱ received only iron supplementation. All the biochemical and hematological parameters were estimated after one month of treatment. RESULTS: In anemic H pylori infected patients, while MDA (5.41 ± 2.16 vs 2.26 ± 0.50; P < 0.05) and fructosamine (2.64 ± 0.93 vs 1.60 ± 0.35; P < 0.05) were significantly increased, iron (32.72 ± 14.93 vs 110.25 ± 26.58; P < 0.05), hemoglobin (6.9 ± 2.6 vs 12.66 ± 0.74; P < 0.05) and ferritin (28.82 ± 16.27 vs 140.43 ± 30.72; P < 0.05) levels were significantly decreased compared with the controls. With partial correlation analysis, fructosamine was found to have a significant positive correlation with MDA. In GroupⅠ, while MDA level decreased significantly (3.11 ± 1.73 vs 5.50 ± 2.46; P < 0.05), there was a significant increase in iron (84.09 ± 29.51 vs 36.09 ± 17.81; P < 0.05), hemoglobin (10.40 ± 1.11 vs 7.42 ± 1.90; P < 0.05) and ferritin (116.91 ± 63.34 vs 30.46 ± 17.81; P < 0.05) levels after one month. There was no significant change in the levels of fructosamine in groupⅠafter treatment. Similarly, no significant alterations were noted in the levels of MDA, fructosamine, hemoglobin or ferritin in Group Ⅱ patients after one month of treatment.CONCLUSION: An increased level of fructosamine and MDA was found in anemic H pylori infected patients. Present data supports the premise that lipid peroxides per se do play a role in the glycation of plasma proteins. Furthermore, the findings from this study indicate that treatment for both anemia and H pylori infections is required for lowering the levels of lipid peroxides in these patients.
基金supported by the Key Projects of Scientific Research Funds from Health Department of Hubei Province(No.JX5A04),China
文摘Under global cerebral ischemia, the effect of different brain temperature on cerebral ischemic injury was studied. Male Sprague-Dawley rats were divided into normothermic(37–38°C) ischemia, mild hypothermic(31–32°C) ischemia, hyperthermic(41–42°C) ischemia and sham-operated groups. Global cerebral ischemia was established using the Pulsinelli four-vessel occlusion model and brain temperature was maintained at defined level for 60 min after 20-min ischemia. The expression of c-fos protein and the levels of malondialdehyde(MDA) and lactate in brain regions were detected by immunochemistry and spectrophotometrical methods, respectively. C-fos positive neurons were found in the hippocampus and cerebral cortex after cerebral ischemia reperfusion. Mild hypothermia increased the expression of c-fos protein in both areas, whereas hyperthermia decreased the expression of c-fos protein in the hippocampus at 24 h reperfusion, and the cerebral cortex at 48 h reperfusion when compared to normothermic conditions. In normothermic, mild hypothermic and hyperthermic ischemia groups, the levels of MDA and lactate in brain tissue were increased at 24, 48 and 72 h reperfusion following 20-min ischemia as compared with the sham-operated group(P<0.01). The levels of MDA and lactate in mild hypothermic group were significantly lower than those in normothermic group(P<0.01). It is suggested that brain temperature influences the translation of the immunoreactive protein product of c-fos after global cerebral ischemia, and MDA and lactate are also affected by hypothermia and hyperthermia.
基金Supported by the National Natural Science Foundation of China(Nos.30570404 and 30670458)the Natural Science Foundation of Jilin Province,China(No.20050561)
文摘Effect of Siqi decoction on myocardial ischemia is to prevent cardiac myocyte membrane from damage associated with oxygen free radicals related to NO. To research the regulatoin of the content of malondialdehyde by Siqi decoction, an index of lipid peroxidation, via increasing activity of superoxide dismutase in blood serum of rats with Myocardial Ischemia, the model of myocardium ischemia was made in Wistar rats with posterior pituitary injection through vein in tail. Siqi decoction, Diaoxinxuekang(DK) and Fufangdanshenpian(FD), the latter two drugs of which are effective TCM drugs of anti-myocardial ischemia at present, were administrated to the rats with myocar-dium ischemia for 5 days to compare the effect of them on myocardium ischemia as reference drugs via measuring the changes of the content of malondialdehyde and the activity of superoxide dismutase in the rat blood serum with myocardial ischemia. There were a remarkable increase in the activity of superoxide dismutase and a decrease in the content of malondialdehyde in the serum of the rats administered Siqi decoction compared with those of the rats in control group, p<0.05. The contents of MDA in the serum of the prevention group rats in the experiments are lower than those of the cure group rats. Anti-Myocardium Ischemia mechanism of Siqi decoction is the regulation of the content of malondialdehyde via increasing activity of superoxide dismutase in the serum of Rats with myocardial ischemia and stimulating the activity of NOS in serum so as to increase NO concentration.
基金Supported by the National Natural Science Foundation of China(No.30270235)
文摘The antioxidant enzyme activity and malondialdehyde(MDA) content of Cephalothrix hongkongiensis were studied to assess variations in the biochemical/physiological parameters of nemerteans under heavy metal stress.Worms were exposed to copper,zinc and cadmium solutions at different concentrations,and the activity of three antioxidant enzymes,catalase(CAT),superoxide dismutase(SOD),and glutathione peroxidase(GPX),and MDA content were measured.The results show that the activity of each enzyme changed immediately after exposure to heavy metals.CAT was invariably inhibited throughout the experimental period,while the SOD activity was significantly elevated by exposure to Cu^(2+) for 48h,but then decreased.SOD was inhibited by Zn^(2+) during the first 12h of exposure,but activated when exposed for longer periods.Under Cd^(2+) stress,SOD activity decreased within 72h.GPX activity varied greatly,being significantly increased by both Cu^(2+) and Zn^(2+),but significantly inhibited by Cd^(2+) in the first 12-24h after exposure.MDA content increased on Cu^(2+) exposure,but normally decreased on Zn^(2+) exposure.MDA content followed an increase-decrease-increase pattern under Cd^(2+) stress.In conclusion,the antioxidant system of this nemertean is sensitive to heavy metals,and its CAT activity may be a potential biomarker for monitoring heavy metal levels in the environment.
文摘AIM: To study the protective effect of a natural antioxi- dant, melatonin, against multistress condition induced lipid peroxidation via determination of gastric damage and plasma malondialdehyde (MDA) level by high perfor- mance liquid chromatography in rats. METHODS: We compared indomethacin-induced gas- tric damage and MDA plasma level in three groups of rats: unoperated, bile duct ligated and sham-operated and evaluated the role of the melatonin on gastric dam- age and plasma MDA level. Indomethacin and melatonin were injected intraperitoneally in doses of 50 mg/kg and 20 mg/kg, respectively. Animals were killed 4 h after in- domethacin injection. RESULTS: Indomethacin induced more severe gastric damage and plasma MDA level in bile duct ligated ani- mals was significantly higher (3.1 ± 0.04 μmol/L) than sham (2.8 ± 0.04 μmol/L) and unoperated animals (1.4 ± 0.08 μmol/L). Pretreatment with melatonin reduced indomethacin-induced gastric damage and plasma MDA level. CONCLUSION: Considering the results of this study, we suggest that in multistress conditions the intensity of gastric damage and the plasma MDA level are great and melatonin reduces the negative effect of lipid peroxida- tion and cell damage by oxidative stress in multistress conditions due to its antioxidizing activity.
文摘[Objective] To investigate the relationship between perinatal diseases and contents of superoxide dismutase (SOD) and malondialdehyde (MDA) in serum of dairy cows. [Method] A total of 38 dairy cows including 22 healthy cows, five cows with ketosis, four cows with fatty liver and seven cows with retained fatal placenta in perinatal period were selected. The contents of SOD and MDA in serum were respectively determined at different times ante partum and post partum. [Result] In the dairy cows with perinatal diseases, the SOD activity is lower and the MDA content was higher than that in the healthy dairy cows during perinatal period. [Conclusion] The antioxidant activity of dairy cows with perinatal diseases is lower than that of healthy dairy cows during perinatal period.
文摘Objective: To determine the concentration of malondialdehyde (MDA), product of lipid peroxidation, in seminal plasma and evalu ate its significance. Methods: Ninety-three cases of infertile pa tients were divided into the obstructive azoospermic (12 cases), the non-obstructive azoospermic (15 cases), the oligozoospermic (21 cases), the asthenozoospermic (19 cases), the oligoastheno zoospermic (16 cases) and the oligoasthenoteratozoospermic (10) groups. Eighteen fertile males served as the controls. MDA con centration was assessed by high-performance liquid chromatogra phy (HPLC). Results: With the exception of the obstructive azoospermic group, the MDA concentration in the seminal plasma was significantly different between the control group and the infer tile groups (P<0.01) as well as between the different infertile groups. Conclusion: Determination of MDA concentration in the seminal plasma is helpful to the diagnosis of male infertility induced by overproduction of reactive oxygen species.
