The open reading frame(ORF)of hAPOA1 was inserted into the prokaryotic expression vector pGEX-4T-1to construct the recombinant plasmid pGEX-4T-1-hAPOA1,which was then transformed into Escherichiacolistrain BL21.The ex...The open reading frame(ORF)of hAPOA1 was inserted into the prokaryotic expression vector pGEX-4T-1to construct the recombinant plasmid pGEX-4T-1-hAPOA1,which was then transformed into Escherichiacolistrain BL21.The expression of target fusion protein was induced with isopropylβ-D-1-thiogalactopyranoside(IPTG).The purified fusion protein in inclusion bodies was used to immunize New Zealand white rabbits to prepare hAPOA1 antiserum and the antibody titer was detected with indirect enzyme-linked immunosorbent assay(ID-ELISA).ID-ELISA and Western Blot proved that rabbit polyclonal antibody with a high titer of 1∶40 000 was produced,which may bring considerable economic benefits.展开更多
Objective:To investigate the morphological changes of the neuronal neurites in diabetic rabbit brain. Methods: Twenty- four New Zealand White rabbits were divided into 2 groups: control group and type Ⅱ diabetic grou...Objective:To investigate the morphological changes of the neuronal neurites in diabetic rabbit brain. Methods: Twenty- four New Zealand White rabbits were divided into 2 groups: control group and type Ⅱ diabetic group induced by high - carbohydrate and high- fat diet. The levels of blood sugar and insulin were detected at week 0(w0), w4, w8, w13, w18, w23 and w28. Brain tissue was stained by Nissl staining and immunolistochemistry with a specific antibody to neurofilament proteins. Result: In diabetic rabbits, the amount of large pyramidal neuron was significantly reduced, and neuronal neurites became swollen, whorled, disrupted and changed in caliber. In hippocampus CA1 region neurofilament staining was very weak. Conclusion: Neurotoxicity of chronic hyperglycemia might be relevant to vascular chronic complications, which affected the expression of NF and led to neurophysiological and structural changes in the brain of rabbits with type Ⅱ diabetes.展开更多
基金Supported by the Agricultural Science Independent Innovation Fund Jiangsu Province[CX(16)1326]
文摘The open reading frame(ORF)of hAPOA1 was inserted into the prokaryotic expression vector pGEX-4T-1to construct the recombinant plasmid pGEX-4T-1-hAPOA1,which was then transformed into Escherichiacolistrain BL21.The expression of target fusion protein was induced with isopropylβ-D-1-thiogalactopyranoside(IPTG).The purified fusion protein in inclusion bodies was used to immunize New Zealand white rabbits to prepare hAPOA1 antiserum and the antibody titer was detected with indirect enzyme-linked immunosorbent assay(ID-ELISA).ID-ELISA and Western Blot proved that rabbit polyclonal antibody with a high titer of 1∶40 000 was produced,which may bring considerable economic benefits.
基金These authors contribute equally to this work.Supported by the Hunan science fourndation(JJY2069)
文摘Objective:To investigate the morphological changes of the neuronal neurites in diabetic rabbit brain. Methods: Twenty- four New Zealand White rabbits were divided into 2 groups: control group and type Ⅱ diabetic group induced by high - carbohydrate and high- fat diet. The levels of blood sugar and insulin were detected at week 0(w0), w4, w8, w13, w18, w23 and w28. Brain tissue was stained by Nissl staining and immunolistochemistry with a specific antibody to neurofilament proteins. Result: In diabetic rabbits, the amount of large pyramidal neuron was significantly reduced, and neuronal neurites became swollen, whorled, disrupted and changed in caliber. In hippocampus CA1 region neurofilament staining was very weak. Conclusion: Neurotoxicity of chronic hyperglycemia might be relevant to vascular chronic complications, which affected the expression of NF and led to neurophysiological and structural changes in the brain of rabbits with type Ⅱ diabetes.
