Acellular matrix(ACM)has been widely used as a biomaterial.As the main component of ACM,collagen type and content show influence on the material properties.In this research,the collagen in ACM from different tissues o...Acellular matrix(ACM)has been widely used as a biomaterial.As the main component of ACM,collagen type and content show influence on the material properties.In this research,the collagen in ACM from different tissues of pig were determined by detection of marker peptides.The marker peptides of Type Ⅰ and Ⅲ collagen were identified from the digested collagen standards using ions trap mass spectrometry(LCQ).The relationship between the abundance of marker peptide and collagen concentration was established using triple quadrupole mass spectrometer(TSQ).The contents of Type Ⅰ and Ⅲ collagen in ACM from different tissues were determined.The method was further verified by hydroxyproline determination.The results showed that,the sum of Type Ⅰ and Ⅲ collagen contents in the ACM from small intestinal submucosa,dermis and Achilles tendon of pig were about 87.59,81.41 and 61.13%,respectively,which were close to the total collagen contents in these tissues.The results proved that this method could quantitatively detect the collagen with different types in the ACM of various tissues.展开更多
基金supported by the National Key Technology R&D Programs of China(2018YFA0108200 and 2018YFC1106400)Beijing Nova Program(Z181100006218013,XX2018008)from the Beijing Municipal Science and Technology Commission+1 种基金Key scientific research project plan of colleges and universities in Henan Province(17A530002)the Guangzhou People’s Livelihood Science and Technology Project of China(201803010086).
文摘Acellular matrix(ACM)has been widely used as a biomaterial.As the main component of ACM,collagen type and content show influence on the material properties.In this research,the collagen in ACM from different tissues of pig were determined by detection of marker peptides.The marker peptides of Type Ⅰ and Ⅲ collagen were identified from the digested collagen standards using ions trap mass spectrometry(LCQ).The relationship between the abundance of marker peptide and collagen concentration was established using triple quadrupole mass spectrometer(TSQ).The contents of Type Ⅰ and Ⅲ collagen in ACM from different tissues were determined.The method was further verified by hydroxyproline determination.The results showed that,the sum of Type Ⅰ and Ⅲ collagen contents in the ACM from small intestinal submucosa,dermis and Achilles tendon of pig were about 87.59,81.41 and 61.13%,respectively,which were close to the total collagen contents in these tissues.The results proved that this method could quantitatively detect the collagen with different types in the ACM of various tissues.
