目的探讨单核细胞单层实验(monocyte monolayer assay,MMA)是否能够用于IgG抗-M相关胎儿新生儿溶血病(hemolytic disease of fetus and newborn,HDFN)的预测。方法选取8例含有IgG抗-M的孕妇并采集血浆标本,其中有胎儿水肿等严重临床症...目的探讨单核细胞单层实验(monocyte monolayer assay,MMA)是否能够用于IgG抗-M相关胎儿新生儿溶血病(hemolytic disease of fetus and newborn,HDFN)的预测。方法选取8例含有IgG抗-M的孕妇并采集血浆标本,其中有胎儿水肿等严重临床症状及无明显临床症状的各4例;8份血浆用二硫苏糖醇(dithiothreitol,DTT)灭活,与M抗原阳性红细胞孵育致敏后,将致敏红细胞与单核细胞混合进行吞噬试验,同时设立阳性及阴性对照,并计算吞噬率;采用t检验对2组吞噬率进行比较。结果4例发生严重抗-M相关HDFN的孕妇的MMA吞噬率分别为15.37%、13.05%、9.17%和24.50%,均值为15.52%;检出IgG抗-M但未发生HDFN的孕妇,其MMA吞噬率分别为8.74%、11.07%、5.12%和6.23%,均值为7.79%,2组吞噬率无差异(P>0.05)。2组吞噬率分别与阴性对照比较均无差异(P>0.05),但均明显低于阳性对照(P<0.05)。结论IgG抗-M介导单核细胞吞噬的能力较低,提示抗-M导致胎儿水肿的机制可能并非红细胞被吞噬破坏而发生的溶血,因此体外单核细胞单层实验可能不适用于IgG抗-M相关HDFN的预测。对于检出IgG抗-M的孕妇,现仍需通过定期监测胎儿大脑中动脉血流,来判断胎儿宫内贫血情况。展开更多
This article presents a 2017 LiDAR-DEM guided 1-m resolution examination of field-surveyed elevation and soil property variations (5 × 5 m spacings) conducted in 1977 across a hummocky New Brunswick field used fo...This article presents a 2017 LiDAR-DEM guided 1-m resolution examination of field-surveyed elevation and soil property variations (5 × 5 m spacings) conducted in 1977 across a hummocky New Brunswick field used for potato production. This examination revealed that the field incurred minor elevation differences likely due to upslope erosion as revealed through increasing Sand % and CF % with increasing elevation, and increasing Silt % along low-lying areas. Soil moisture, field capacity, permanent wilting and nitrate nitrogen (NO<sub>3</sub>-N) also increased at downslope locations. Directly as well as indirectly, soil pH, ammonium nitrogen (NH<sub>4</sub>-N), Caesium<sup>137</sup> (Cs<sup>137</sup>) and Mehlich-3 extracted Ca, Mg, K, Fe, Mn, Cu, and Zn were likewise affected by topographic location. Factor analyzing these variables led to: 1) a Soil Loss Factor that captured 24% of the textural variations;2) a Soil-Cropping Factor accounting for 16% of the N, P, K, Ca, Mg, Mn variations;3) a Soil Organic Matter (SOM) Factor relating 9% of the in-field variations for SOM, Fe, Zn, Cu to via organo-metal complexation and low NO<sub>3</sub>-N retention. Many of the topographic variations increased or decreased with the metric DEM-projected depth-to-water index (DTW) index. This index was set to 0 along DEM-derived flow channels with minimum upslope flow-accumulation areas of 0.1, 0.25, 0.5, 1 or 4 ha. Among these, the DTW > 4 ha threshold was useful for reproducing the textural variations, while the DTW > 0.25 ha threshold assisted in capturing trends pertaining to moisture retention and elemental concentrations.展开更多
母婴血型不合新生儿溶血病（maternal-fetal blood group incompatibility hemolytic disease of the new born，HDN）是由于母婴血型不合导致的胎儿或新生儿免疫性溶血性疾病。临床主要分为ABO血型不合和Rh血型不合，而MN系统HDN非常...母婴血型不合新生儿溶血病（maternal-fetal blood group incompatibility hemolytic disease of the new born，HDN）是由于母婴血型不合导致的胎儿或新生儿免疫性溶血性疾病。临床主要分为ABO血型不合和Rh血型不合，而MN系统HDN非常罕见。血清学检查包括血生化检测和新生儿溶血3项试验（抗球蛋白试验、游离抗体试验和抗体释放试验）。在某些特殊情况下，如患儿重度贫血、抗体效价较低、抗体被消耗、标本严重溶血等，用患儿血标本的结果不足以诊断HDN时，可以通过其父母的血标本进一步检测来辅助诊断。近日我院收治1名重度贫血的新生儿，通过其父母标本确认为抗-M引起的HDN，现报道如下。展开更多
目的母婴血型不合导致的胎儿及新生儿溶血病(hemolytic disease of the fetus and newborn,HDFN)多见于ABO及Rh系统,其他系统引起的少见。文中介绍了1例罕见的由抗-M抗体引起的严重胎儿溶血导致多次宫内死胎的案例,旨在加强对IgG型抗-M...目的母婴血型不合导致的胎儿及新生儿溶血病(hemolytic disease of the fetus and newborn,HDFN)多见于ABO及Rh系统,其他系统引起的少见。文中介绍了1例罕见的由抗-M抗体引起的严重胎儿溶血导致多次宫内死胎的案例,旨在加强对IgG型抗-M抗体临床重要性的认识。方法复习该经产妇的5次妊娠史及胎儿情况,对其及其丈夫血型进行血清学及基因分型,并对其血型抗体进行筛查及效价检测。结果该经产妇为NN血型,其丈夫为MM血型;其血清中检测出抗-M抗体,多次抗体效价测定值波动范围为1∶8至1∶64。结论通过对本例血型基因的分析,确诊为抗-M抗体引起的HDFN,提示对于原因不明的多次死胎、流产等,需要考虑到抗-M抗体或其他血型抗体引起的可能性。展开更多
文摘目的探讨单核细胞单层实验(monocyte monolayer assay,MMA)是否能够用于IgG抗-M相关胎儿新生儿溶血病(hemolytic disease of fetus and newborn,HDFN)的预测。方法选取8例含有IgG抗-M的孕妇并采集血浆标本,其中有胎儿水肿等严重临床症状及无明显临床症状的各4例;8份血浆用二硫苏糖醇(dithiothreitol,DTT)灭活,与M抗原阳性红细胞孵育致敏后,将致敏红细胞与单核细胞混合进行吞噬试验,同时设立阳性及阴性对照,并计算吞噬率;采用t检验对2组吞噬率进行比较。结果4例发生严重抗-M相关HDFN的孕妇的MMA吞噬率分别为15.37%、13.05%、9.17%和24.50%,均值为15.52%;检出IgG抗-M但未发生HDFN的孕妇,其MMA吞噬率分别为8.74%、11.07%、5.12%和6.23%,均值为7.79%,2组吞噬率无差异(P>0.05)。2组吞噬率分别与阴性对照比较均无差异(P>0.05),但均明显低于阳性对照(P<0.05)。结论IgG抗-M介导单核细胞吞噬的能力较低,提示抗-M导致胎儿水肿的机制可能并非红细胞被吞噬破坏而发生的溶血,因此体外单核细胞单层实验可能不适用于IgG抗-M相关HDFN的预测。对于检出IgG抗-M的孕妇,现仍需通过定期监测胎儿大脑中动脉血流,来判断胎儿宫内贫血情况。
文摘This article presents a 2017 LiDAR-DEM guided 1-m resolution examination of field-surveyed elevation and soil property variations (5 × 5 m spacings) conducted in 1977 across a hummocky New Brunswick field used for potato production. This examination revealed that the field incurred minor elevation differences likely due to upslope erosion as revealed through increasing Sand % and CF % with increasing elevation, and increasing Silt % along low-lying areas. Soil moisture, field capacity, permanent wilting and nitrate nitrogen (NO<sub>3</sub>-N) also increased at downslope locations. Directly as well as indirectly, soil pH, ammonium nitrogen (NH<sub>4</sub>-N), Caesium<sup>137</sup> (Cs<sup>137</sup>) and Mehlich-3 extracted Ca, Mg, K, Fe, Mn, Cu, and Zn were likewise affected by topographic location. Factor analyzing these variables led to: 1) a Soil Loss Factor that captured 24% of the textural variations;2) a Soil-Cropping Factor accounting for 16% of the N, P, K, Ca, Mg, Mn variations;3) a Soil Organic Matter (SOM) Factor relating 9% of the in-field variations for SOM, Fe, Zn, Cu to via organo-metal complexation and low NO<sub>3</sub>-N retention. Many of the topographic variations increased or decreased with the metric DEM-projected depth-to-water index (DTW) index. This index was set to 0 along DEM-derived flow channels with minimum upslope flow-accumulation areas of 0.1, 0.25, 0.5, 1 or 4 ha. Among these, the DTW > 4 ha threshold was useful for reproducing the textural variations, while the DTW > 0.25 ha threshold assisted in capturing trends pertaining to moisture retention and elemental concentrations.
文摘母婴血型不合新生儿溶血病（maternal-fetal blood group incompatibility hemolytic disease of the new born，HDN）是由于母婴血型不合导致的胎儿或新生儿免疫性溶血性疾病。临床主要分为ABO血型不合和Rh血型不合，而MN系统HDN非常罕见。血清学检查包括血生化检测和新生儿溶血3项试验（抗球蛋白试验、游离抗体试验和抗体释放试验）。在某些特殊情况下，如患儿重度贫血、抗体效价较低、抗体被消耗、标本严重溶血等，用患儿血标本的结果不足以诊断HDN时，可以通过其父母的血标本进一步检测来辅助诊断。近日我院收治1名重度贫血的新生儿，通过其父母标本确认为抗-M引起的HDN，现报道如下。
文摘目的母婴血型不合导致的胎儿及新生儿溶血病(hemolytic disease of the fetus and newborn,HDFN)多见于ABO及Rh系统,其他系统引起的少见。文中介绍了1例罕见的由抗-M抗体引起的严重胎儿溶血导致多次宫内死胎的案例,旨在加强对IgG型抗-M抗体临床重要性的认识。方法复习该经产妇的5次妊娠史及胎儿情况,对其及其丈夫血型进行血清学及基因分型,并对其血型抗体进行筛查及效价检测。结果该经产妇为NN血型,其丈夫为MM血型;其血清中检测出抗-M抗体,多次抗体效价测定值波动范围为1∶8至1∶64。结论通过对本例血型基因的分析,确诊为抗-M抗体引起的HDFN,提示对于原因不明的多次死胎、流产等,需要考虑到抗-M抗体或其他血型抗体引起的可能性。
