试验考察宰前添加天冬氨酸镁(MgAsp)与维生素D3(VD3)对试猪血液理化指标、猪肉品质以及μ-Calpain和Calpastatin基因mRNA含量的影响。选体重约90 kg DLY杂交猪48头,随机分到4个处理:对照、加MgAsp、加VD3和加MgAsp与VD3。试验期为9 d,...试验考察宰前添加天冬氨酸镁(MgAsp)与维生素D3(VD3)对试猪血液理化指标、猪肉品质以及μ-Calpain和Calpastatin基因mRNA含量的影响。选体重约90 kg DLY杂交猪48头,随机分到4个处理:对照、加MgAsp、加VD3和加MgAsp与VD3。试验期为9 d,试验结束后选取24头进行屠宰。结果表明:宰前短期添加MgAsp和VD3对育肥猪胴体品质无影响;与对照相比,添加MgAsp提高血清镁含量(P<0.05),添加VD3提高血清磷(P<0.05)和钙的含量,MgAsp和VD3联合添加可进一步提高血清镁含量(P<0.01);添加MgAsp降低臀肌L值(P<0.01),并有提高肌肉pH值和降低滴水损失的趋势,提高了μ-Calpain与Calpastatin mRNA的比值,改善了嫩度;添加VD3降低了臀肌的L24h值(P<0.05),有提高pH和嫩度的趋势;MgAsp和VD3联合添加除对剪切力略有改善而外,对其他肉质指标并没有进一步改善。展开更多
The neuroprotective property of quercetin is well reported against hypoxia and ischemia in past studies.This property of quercetin lies in its antioxidant property with blood-brain barrier permeability and anti-inflam...The neuroprotective property of quercetin is well reported against hypoxia and ischemia in past studies.This property of quercetin lies in its antioxidant property with blood-brain barrier permeability and anti-inflammatory capabilities.μ-Calpain,a calcium ion activated intracellular cysteine protease causes serious cellular insult,leading to cell death in various pathological conditions including hypoxia and ischemic stroke.Hence,it may be considered as a potential drug target for the treatment of hypoxia induced neuronal injury.As the inhibitory property of μ-calpain is yet to be explored in details,hence,in the present study,we investigated the interaction of quercetin with μ-calpain through a molecular dynamics simulation study as a tool through clarifying the molecular mechanism of such inhibition and determining the probable sites and modes of quercetin interaction with the μ-calpain catalytic domain.In addition,we also investigated the structure-activity relationship of quercetin with μ-calpain.Affinity binding of quercetin with μ-calpain had a value of –28.73 k J/mol and a Ki value of 35.87 μM that may be a probable reason to lead to altered functioning of μ-calpain.Hence,quercetin was found to be an inhibitor of μ-calpain which might have a possible therapeutic role in hypoxic injury.展开更多
【目的】研究蕨麻正丁醇部位对大鼠心肌细胞缺氧所致钙超载和细胞凋亡的抑制作用,并通过研究钙蛋白酶μ-calpain表达的变化,探讨其可能的机制。【方法】建立原代心肌细胞缺氧模型,设正常对照组、缺氧损伤组、维拉帕米组(5μmol/L)及蕨...【目的】研究蕨麻正丁醇部位对大鼠心肌细胞缺氧所致钙超载和细胞凋亡的抑制作用,并通过研究钙蛋白酶μ-calpain表达的变化,探讨其可能的机制。【方法】建立原代心肌细胞缺氧模型,设正常对照组、缺氧损伤组、维拉帕米组(5μmol/L)及蕨麻正丁醇部位高、中、低浓度组(250.0、62.5、15.6μg/ml)。采用荧光分光光度法检测心肌细胞内钙离子变化,Hoechst3342/PI染色检测心肌细胞凋亡,RT-PCR法检测μ-calpain m RNA水平,Western blotting方法和免疫组织化学方法检测μ-calpain蛋白。【结果】缺氧损伤组心肌细胞内游离钙离子的浓度较正常对照组明显增加(P<0.01),细胞凋亡严重,μ-calpain m RNA及蛋白表达显著上调(P<0.01),蕨麻正丁醇部位250.0、62.5μg/ml组心肌细胞内游离钙离子的浓度较缺氧损伤组明显降低,μ-calpain m RNA及蛋白表达均显著下调(P<0.01),细胞凋亡程度明显减轻。【结论】蕨麻正丁醇部位可明显抑制缺氧所致心肌细胞游离钙离子浓度增加及μ-calpain的上调,减少心肌细胞晚期凋亡和死亡,提示蕨麻正丁醇部位对心肌细胞缺氧损伤的保护作用可能与其抑制μ-calpain的表达有关。展开更多
文摘试验考察宰前添加天冬氨酸镁(MgAsp)与维生素D3(VD3)对试猪血液理化指标、猪肉品质以及μ-Calpain和Calpastatin基因mRNA含量的影响。选体重约90 kg DLY杂交猪48头,随机分到4个处理:对照、加MgAsp、加VD3和加MgAsp与VD3。试验期为9 d,试验结束后选取24头进行屠宰。结果表明:宰前短期添加MgAsp和VD3对育肥猪胴体品质无影响;与对照相比,添加MgAsp提高血清镁含量(P<0.05),添加VD3提高血清磷(P<0.05)和钙的含量,MgAsp和VD3联合添加可进一步提高血清镁含量(P<0.01);添加MgAsp降低臀肌L值(P<0.01),并有提高肌肉pH值和降低滴水损失的趋势,提高了μ-Calpain与Calpastatin mRNA的比值,改善了嫩度;添加VD3降低了臀肌的L24h值(P<0.05),有提高pH和嫩度的趋势;MgAsp和VD3联合添加除对剪切力略有改善而外,对其他肉质指标并没有进一步改善。
文摘The neuroprotective property of quercetin is well reported against hypoxia and ischemia in past studies.This property of quercetin lies in its antioxidant property with blood-brain barrier permeability and anti-inflammatory capabilities.μ-Calpain,a calcium ion activated intracellular cysteine protease causes serious cellular insult,leading to cell death in various pathological conditions including hypoxia and ischemic stroke.Hence,it may be considered as a potential drug target for the treatment of hypoxia induced neuronal injury.As the inhibitory property of μ-calpain is yet to be explored in details,hence,in the present study,we investigated the interaction of quercetin with μ-calpain through a molecular dynamics simulation study as a tool through clarifying the molecular mechanism of such inhibition and determining the probable sites and modes of quercetin interaction with the μ-calpain catalytic domain.In addition,we also investigated the structure-activity relationship of quercetin with μ-calpain.Affinity binding of quercetin with μ-calpain had a value of –28.73 k J/mol and a Ki value of 35.87 μM that may be a probable reason to lead to altered functioning of μ-calpain.Hence,quercetin was found to be an inhibitor of μ-calpain which might have a possible therapeutic role in hypoxic injury.
文摘【目的】研究蕨麻正丁醇部位对大鼠心肌细胞缺氧所致钙超载和细胞凋亡的抑制作用,并通过研究钙蛋白酶μ-calpain表达的变化,探讨其可能的机制。【方法】建立原代心肌细胞缺氧模型,设正常对照组、缺氧损伤组、维拉帕米组(5μmol/L)及蕨麻正丁醇部位高、中、低浓度组(250.0、62.5、15.6μg/ml)。采用荧光分光光度法检测心肌细胞内钙离子变化,Hoechst3342/PI染色检测心肌细胞凋亡,RT-PCR法检测μ-calpain m RNA水平,Western blotting方法和免疫组织化学方法检测μ-calpain蛋白。【结果】缺氧损伤组心肌细胞内游离钙离子的浓度较正常对照组明显增加(P<0.01),细胞凋亡严重,μ-calpain m RNA及蛋白表达显著上调(P<0.01),蕨麻正丁醇部位250.0、62.5μg/ml组心肌细胞内游离钙离子的浓度较缺氧损伤组明显降低,μ-calpain m RNA及蛋白表达均显著下调(P<0.01),细胞凋亡程度明显减轻。【结论】蕨麻正丁醇部位可明显抑制缺氧所致心肌细胞游离钙离子浓度增加及μ-calpain的上调,减少心肌细胞晚期凋亡和死亡,提示蕨麻正丁醇部位对心肌细胞缺氧损伤的保护作用可能与其抑制μ-calpain的表达有关。
