目的:1,25-二羟维生素D3[1,25-(OH)2D3]对连续多次小剂量链脲菌素(the mu ltip le low dose streptozotoc in,MLDS)诱导的自身免疫性糖尿病小鼠的预防作用及其机制初探。方法:小鼠分为三组。正常对照组:连续5天腹腔注射与糖尿病组等容...目的:1,25-二羟维生素D3[1,25-(OH)2D3]对连续多次小剂量链脲菌素(the mu ltip le low dose streptozotoc in,MLDS)诱导的自身免疫性糖尿病小鼠的预防作用及其机制初探。方法:小鼠分为三组。正常对照组:连续5天腹腔注射与糖尿病组等容量柠檬酸盐缓冲液;糖尿病组:连续5天腹腔注射STZ(40mg.kg-1),以血糖水平持续高于16.7mmol/L为成模标准;预防组:先隔日腹腔注射1,25-(OH)2D3(5μg.kg-1),共15次,然后再连续5天腹腔注射STZ(40mg.kg-1)。实验结束后各组动物处死收集血清并采集胰腺检测诱导型一氧化氮合酶(iNOS)活性及血清胰岛素水平。结果:MLDS诱发的自身免疫性糖尿病模型在第四周基本建成。MLDS使小鼠血糖、血清及胰腺iNOS活性升高,血清胰岛素水平下调;预防组小鼠注射STZ前给予1,25-(OH)2D3有明显降血糖和上调血清胰岛素水平作用,同时抑制血清及胰腺iNOS活性,与糖尿病组比较有显著性差异(P<0.05)。结论:1,25-(OH)2D3可有效预防MLDS诱导的自身免疫性糖尿病的发生。该效应可能与1,25-(OH)2D3抑制iNOS活性有关。展开更多
The objective of the study was to determine the role of vitamin D3(VD3) in regulating adaptation and mechanism of rats to low-phosphorus(P) diets. Rats were assigned to 4 diets containing 0.2%, 0.4%, 0.6%,or 0.8% P co...The objective of the study was to determine the role of vitamin D3(VD3) in regulating adaptation and mechanism of rats to low-phosphorus(P) diets. Rats were assigned to 4 diets containing 0.2%, 0.4%, 0.6%,or 0.8% P consisting of 5 replicate cages with 6 rats per replicate cage and fed for 7 days. Four rats from each replicate cage were treated with ethane-1-hydroxy-1,1-diphosphonicacid, tetrasodium salt(EHDP)and 2 rats remained untreated. Twelve hours prior to preparation on d 7, two of the EHDP-treated rats received an intraperitoneal injection of VD3 [1,25-(OH)_2 D_3] at 600 ng per kg body weight, while two rats did not receive the injection. Rats that did not receive VD_3 injection had decreased(P < 0.001) P absorption, but injection of VD3 resulted in increased(P < 0.001) absorption. The effect of VD3 injection was greater(P < 0.001) for rats fed 0.2% P diet than rats fed 0.8% P diet in ileum. Sodium dependent phosphate cotransporter type IIb(Na/Pi-IIb) and 25-hydroxyvitamin D 1-α hydroxylase(CYP27 B1) mRNA level showed the same trend with P absorption. Serum concentration of VD3 and la-hydroxylase activity in rats fed 0.2% P diet were lower than those fed 0.8% P diet. The injection of VD3 increased(P < 0.001)serum concentration of VD3 and la-hydroxylase activity. Thus, VD3 increased Na/Pi-Ⅱb and CYP27 B1 mRNA level and improved serum concentration of VD3 and la-hydroxylase activity in rats fed low-P diets.展开更多
文摘目的:1,25-二羟维生素D3[1,25-(OH)2D3]对连续多次小剂量链脲菌素(the mu ltip le low dose streptozotoc in,MLDS)诱导的自身免疫性糖尿病小鼠的预防作用及其机制初探。方法:小鼠分为三组。正常对照组:连续5天腹腔注射与糖尿病组等容量柠檬酸盐缓冲液;糖尿病组:连续5天腹腔注射STZ(40mg.kg-1),以血糖水平持续高于16.7mmol/L为成模标准;预防组:先隔日腹腔注射1,25-(OH)2D3(5μg.kg-1),共15次,然后再连续5天腹腔注射STZ(40mg.kg-1)。实验结束后各组动物处死收集血清并采集胰腺检测诱导型一氧化氮合酶(iNOS)活性及血清胰岛素水平。结果:MLDS诱发的自身免疫性糖尿病模型在第四周基本建成。MLDS使小鼠血糖、血清及胰腺iNOS活性升高,血清胰岛素水平下调;预防组小鼠注射STZ前给予1,25-(OH)2D3有明显降血糖和上调血清胰岛素水平作用,同时抑制血清及胰腺iNOS活性,与糖尿病组比较有显著性差异(P<0.05)。结论:1,25-(OH)2D3可有效预防MLDS诱导的自身免疫性糖尿病的发生。该效应可能与1,25-(OH)2D3抑制iNOS活性有关。
基金supported by grants from the Nature Science Foundation (31201810, to M H Cao 31572419, to R J Fang)+1 种基金Education Department of Hunan Province(138039, to M H Cao)Innovation Team Funds Of Hunan Province (to J H He)
文摘The objective of the study was to determine the role of vitamin D3(VD3) in regulating adaptation and mechanism of rats to low-phosphorus(P) diets. Rats were assigned to 4 diets containing 0.2%, 0.4%, 0.6%,or 0.8% P consisting of 5 replicate cages with 6 rats per replicate cage and fed for 7 days. Four rats from each replicate cage were treated with ethane-1-hydroxy-1,1-diphosphonicacid, tetrasodium salt(EHDP)and 2 rats remained untreated. Twelve hours prior to preparation on d 7, two of the EHDP-treated rats received an intraperitoneal injection of VD3 [1,25-(OH)_2 D_3] at 600 ng per kg body weight, while two rats did not receive the injection. Rats that did not receive VD_3 injection had decreased(P < 0.001) P absorption, but injection of VD3 resulted in increased(P < 0.001) absorption. The effect of VD3 injection was greater(P < 0.001) for rats fed 0.2% P diet than rats fed 0.8% P diet in ileum. Sodium dependent phosphate cotransporter type IIb(Na/Pi-IIb) and 25-hydroxyvitamin D 1-α hydroxylase(CYP27 B1) mRNA level showed the same trend with P absorption. Serum concentration of VD3 and la-hydroxylase activity in rats fed 0.2% P diet were lower than those fed 0.8% P diet. The injection of VD3 increased(P < 0.001)serum concentration of VD3 and la-hydroxylase activity. Thus, VD3 increased Na/Pi-Ⅱb and CYP27 B1 mRNA level and improved serum concentration of VD3 and la-hydroxylase activity in rats fed low-P diets.
